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Microquantitation of Van Gogh-like Protein 1 by Using Antibody-Conjugated Magnetic Beads
염수정,이신영,이사타스,이미진,박소연,정상철,김경근,김항건 한국바이오칩학회 2019 BioChip Journal Vol.13 No.2
Van Gogh-like protein 1 (VANGL1) is an integral membrane protein that has a decisive effect on invasion, migration, and metastasis of tumor cells in various cancers including colorectal cancer. Elevation of VANGL1 level in cancer tissue is often observed with progressive and metastatic colorectal cancer. Therefore, detection of VANGL1 level in patient specimens can be used as a diagnostic marker for colorectal cancer screening. In this study, we developed magnetic beads that conjugate the VANGL1 antibody, which can be used for quantitative analysis of VANGL1. The procedure for bead preparation was optimized, and detection analysis was validated by using Caco2 and HT29 colorectal cancer cell lysates. Through the procedure, VANGL1 level from cell lysates of Caco2 and HT29 cells were quantified to estimate whether the antibody-conjugated magnetic bead can be used to trace amounts of VANGL1. Results from VANGL1 level obtained by using the antibody-conjugated magnetic beads suggest that the procedure has high precision and sensitivity in analyzing VANGL1. In conclusion, the results indicate that the method is appropriate for microquantitation of VANGL1 from patient specimens.
염수정,구옥재,박정규,이병천,이왕재 사단법인 한국동물생명공학회 2013 Reproductive & developmental biology Vol.37 No.1
In order to investigate genetic stability and gene expression profile after cloning procedure, two groups of cloned pigs were used for swine leukocyte antigen (SLA) gene nucleotide alteration and microarray analyses. Each group was consist of cloned pigs derived from same cell line (n=3 and 4, respectively). Six SLA loci were analyzed for cDNA sequences and protein translations. In total, 16 SLA alleles were identified and there were no evidence of SLA nucleotide alteration. All SLA sequences and protein translations were identical among the each pig in the same group. On the other hand, microarray assay was performed for profiling gene expression of the cloned pigs. In total, 43,603 genes were analyzed and 2,150~4,300 reliably hybridized spots on the each chip were selected for further analysis. Even though the cloned pigs in the same group had identical genetic background, 18.6~47.3% of analyzed genes were differentially expressed in between each cloned pigs. Furthermore, on gene clustering analysis, some cloned pigs showed abnormal physiological phenotypes such as inflammation, cancer or cardiomyopathy. We assumed that individual environmental adaption, sociality and rank in the pen might have induced these different phenotypes. In conclusion, the results of the present study indicate that SLA locus genes appear to be stable following SCNT. However, gene expressions and phenotypes between cloned pigs derived from the same cell line were not identical even under the same rearing conditions.
보청기 귓본 인상재 이물에 의해 유발된 외이도 염증성 육아종 1예
염수정,임혜린,조형호 대한이비인후과학회 2023 대한이비인후과학회지 두경부외과학 Vol.66 No.9
Hearing aids are becoming more popular as the elderly population grows, but complicationsrelated to their use are also increasing. We present a rare case where an ear mold impressionentered the mastoid cavity during fitting, causing delayed inflammation about 10 years later. Unlike most reported cases, this patient did not experience immediate symptoms during theimpression-making process. Our case highlights the importance of thorough physical exami-nation by an otolaryngologist, including assessment of the tympanic membrane and externalauditory canal, when fitting hearing aids to prevent similar complications.
김수정,하소영,최보미,이미영,진종률,염수정,김태완,김영민,이기현 대한혈액학회 2013 Blood Research Vol.48 No.1
Background Anemia is the most common hematologic condition encountered in outpatient clinics. It is often overlooked because it is common among patients; however, anemia is one of the leading indicators of cancer. This study examined the prevalence and characteristics of cancer among anemia patients who visited an outpatient clinic. Methods The data were collected by reviewing the records of an outpatient clinic from January 2007 to December 2011. Results In total, 502 patients (52 males, 450 females) were diagnosed with anemia. Cancer prevalence among anemia patients was 5.57% (25.0%, men; 3.3%, women); further, the most frequently diagnosed cancer was colorectal cancer (22.5%), followed by advanced gastric cancer (16.1%), breast cancer (9.6%), myelodysplastic syndrome (9.6%), cervical cancer (6.4%), renal-cell carcinoma (6.4%), and thyroid cancer (6.4%). The prevalence of cancer was 4.1% in those aged 40‒49 years, 4.2% in the subjects in their fifties, 8.0% in those in their sixties, 21.6% in those in their seventies, and 55.6% in those aged over 80 years. The cancer prevalence among iron deficiency anemia (IDA) patients was 6.18% (28.8%, men; 3.5%, women). The cancer prevalence in postmenopausal and premenopausal female IDA patients was 16.0% and 1.6%, respectively. Conclusion Among anemia patients, male patients aged over 40 years and female patients aged over 60 years, along with postmenopausal female patients, were more likely to be diagnosed with cancer. Consequently, male IDA patients, and female patients aged over 60 years must be carefully evaluated for the possibility of malignancy.
김수정,하소영,최보미,이미영,진종률,염수정,김태완,김영민,이기현 대한혈액학회 2013 Blood Research Vol.48 No.1
Background Anemia is the most common hematologic condition encountered in outpatient clinics. It is often overlooked because it is common among patients; however, anemia is one of the leading indicators of cancer. This study examined the prevalence and characteristics of cancer among anemia patients who visited an outpatient clinic. Methods The data were collected by reviewing the records of an outpatient clinic from January 2007 to December 2011. Results In total, 502 patients (52 males, 450 females) were diagnosed with anemia. Cancer prevalence among anemia patients was 5.57% (25.0%, men; 3.3%, women); further, the most frequently diagnosed cancer was colorectal cancer (22.5%), followed by advanced gastric cancer (16.1%), breast cancer (9.6%), myelodysplastic syndrome (9.6%), cervical cancer (6.4%), renal-cell carcinoma (6.4%), and thyroid cancer (6.4%). The prevalence of cancer was 4.1% in those aged 40‒49 years, 4.2% in the subjects in their fifties, 8.0% in those in their sixties, 21.6% in those in their seventies, and 55.6% in those aged over 80 years. The cancer prevalence among iron deficiency anemia (IDA) patients was 6.18% (28.8%, men; 3.5%, women). The cancer prevalence in postmenopausal and premenopausal female IDA patients was 16.0% and 1.6%, respectively. Conclusion Among anemia patients, male patients aged over 40 years and female patients aged over 60 years, along with postmenopausal female patients, were more likely to be diagnosed with cancer. Consequently, male IDA patients, and female patients aged over 60 years must be carefully evaluated for the possibility of malignancy.