産婦의 看護要求와 看護員의 看護活動과의 比較 硏究

安惠淑 한국간호과학회 1976 Journal of Korean Academy of Nursing Vol.6 No.1

New polymorphic microsatellite markers in the Korean mi-iuy croaker, Miichthys miiuy, and their application to the genetic characterization of wild and farmed populations

안혜숙,김은미,이장욱,김대중,김이청 한국통합생물학회 2012 Animal cells and systems Vol.16 No.1

Eighteen new polymorphic microsatellite markers were developed for the Korean mi-iuy croaker (Miichthys miiuy,Perciformes, Sciaenidae), and allelic variability was compared between a wild population in Mokpo, Korea, and a hatchery population in Tongyeong, Korea. All loci were amplified readily and demonstrated allelic variability, with the number of alleles ranging from 5 to 37 in the wild population, and from 4 to 12 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, to be 0.74 and 0.78 in the hatchery population samples, and 0.79 and 0.86 in the wild samples. These results indicate lower genetic variability in the hatchery population compared with the wild population, and significant genetic differentiation between the wild population and the hatchery samples (FST0.058, PB0.001). These microsatellite loci may be valuable for future population genetic studies, monitoring changes in the genetic variation within stocks in a commercial breeding program, conservation genetics, and molecular assisted selective breeding of the mi-iuy croaker in the future.

Development and characterization of microsatellite markers for an endangered species, Epinephelus bruneus, to establish a conservation program

안혜숙,김재우,이장욱,김신권,이배익,김대중,김이청 한국통합생물학회 2012 Animal cells and systems Vol.16 No.1

Kelp grouper (Epinephelus bruneus Bloch 1793) is a commercially important fish in Korea. In recent years, the catch of kelp grouper in the coastal waters of Korea has significantly declined. Despite its importance, little is known about its genetic diversity and conservation efforts are hampered. In this study, we isolated and characterized 12microsatellite loci using an enrichment method based on magnetic/biotin capture of microsatellite sequences from a size-selected genomic library. All loci were readily amplified and contained TG/CA denucleotide repeats. To characterize each locus, 30 individuals from a natural E. bruneus population in the coastal waters of Jeju Island,Korea, were genotyped. All loci except three, KEm118, KEm154, and KEm219, were polymorphic, with an average of 8.1 alleles per locus (range 218). The mean observed and expected heterozygosities were 0.47 (range 0.191.00)and 0.61 (range 0.290.92), respectively. A significant deviation from Hardy-Weinberg equilibrium was observed at three loci (KEm134, KEm184, and KEm283). These findings will be useful for effective monitoring and management of genetic variation of kelp grouper as well as for the implementation of a fisheries conservation program.

Population genetic structure of Korean pen shell (Atrina pectinata) in Korea inferred from microsatellite marker analysis

안혜숙,이장욱,Chun Mae Dong 한국유전학회 2012 Genes & Genomics Vol.34 No.6

The Korean pen shell Atrina pectinata is a commercially valuable species in Korea. The commercial catch of this fish has decreased continuously since 1990. However, its genetic characteristics have never been studied. In order to explore the population genetic structure of this species, 125 pen shells sampled from three major habitats along the western coast of Korea were genotyped at 21 microsatellite loci. Relatively high levels of genetic variability (mean number of allelic richness (AR) = 14.74; mean hetrerozygosity (He) = 0.849) was found among localities. However, despite the potential of the Korean pen shell to exhibit genetic structure (adults are sedentary),none of the genetic tests applied in this study detected significant genetic differentiation among the samples. The lack of genetic differentiation among samples may be due to high levels of larval dispersal, through passive drift with ocean currents. Alternatively, populations may have diverged too recently for significant genetic differentiation to have become evident. Furthermore, small sample sizes and the limited number of samples may have hampered the detection of genetic structure. The results of this study therefore suggested a lack of genetic structure among the populations of pen shell of Korean waters and should be managed as a single unit. This information on the genetic characteristics of Korean pen shell populations has important implications for the sustainable exploitation of the fishing resources and the preservation of biodiversity.

Molecular identification of Korean catfish (Siluriformes) based on two genetic markers

안혜숙,김미정,이장욱,이완옥 한국유전학회 2012 Genes & Genomics Vol.34 No.6

Eleven species of catfish (Siluriformes) found in Korean freshwater are economically important resources and include five endemic species. However, there are no studies on phylogenetic analysis of all catfish species in Korea at a molecular level. The species-level analysis of catfish species is usually carried out through morphological characters and controversial due to phenotypic variation. In this study, the partial sequences of 16S rRNA and cytochrome b mitochondrial genes were analyzed for species identification and phylogenetic relationships among 11 species of catfish from 10 different rivers in Korea. The nucleotide sequences of 16S rRNA and cytochrome b consisted of 587 and 441 nucleotide base pairs, respectively. Sequence analysis of both genes revealed that the 11 species fell into three distinct groups, which were genetically distinct from each other and exhibited identical phylogenetic resolution. Sequence divergences between congeneric species averaged 1.78% and 7.39% for 16S rRNA and cytochrome b, respectively. The phylogenetic relationships forming well-differentiated clades in the NJ, ML and BI trees were identical for both fragments. This research demonstrates that partial sequences of both the genes can efficiently identify the 11 species of catfish in Korea, indicating the usefulness of mtDNA-based approach in species identification.

다문화시대를 위한 문화교육 교수학습 방법 탐구- 중학교 국어교과서의 고전 소설 ‘홍길동전’을 중심으로 -

안혜숙 강원대학교 국어교육과 2016 語文學報 Vol.36 No.-

Genetic differences between broodstock and offspring of seven-band grouper in a hatchery

안혜숙,조재권,김경민,손맹현,명정인,안철민 한국유전학회 2014 Genes & Genomics Vol.36 No.5

The seven-band grouper (Epinephelus septemfasciatus)is an important fishery resource of a target forprospective aquaculture diversification and maintenance ofstock quality is thus important. To explore the sustainabilityof fry production, genetic variations in 83 sevenbandgroupers from two broodstock and offspring populationsof a hatchery strain were analyzed using 13 polymorphicnuclear microsatellite DNA loci; 133 alleles wereidentified. Allelic variability ranged from 4 to 18 in thebroodstock and from 3 to 11 in the offspring. The averageobserved and expected heterozygosities were 0.669 and0.734 in broodstock and 0.674 and 0.649 in offspring,respectively. Although no statistically significant reductionsin heterozygosity or allelic diversity were evident inoffspring, considerable loss of rare alleles was apparent. The broodstock and offspring populations exhibited significantgenetic differences (FST = 0.033, P\0.001)indicating that genetic drift has likely promoted differentiationbetween the two populations, which may havenegative effects on sustainable fry production. Therefore,genetic variations between broodstock and offspring shouldbe monitored, and inbreeding should be controlled, toensure the success of commercial breeding programs. Ourdata provide a useful genetic basis for future planning ofsustainable culture and management of E. septemfasciatusin fisheries.

Comparative genetic diversity of wild and hatchery-produced Pacific oyster (Crassostrea gigas) populations in Korea using multiplex PCR assays with nine polymorphic microsatellite markers

안혜숙,이장욱,김우진,임현정,김은미,변순규,허영백,박정연,명정인,안철민 한국유전학회 2013 Genes & Genomics Vol.35 No.6

The Pacific oyster, Crassostrea gigas, is the most important and valuable commercial fishery species in Korea. Its farming started 20 years ago and is still rapid expansion in Korea. In this study, to maintain the genetic diversity of this valuable marine resource, possible genetic similarity and differences between the wild population and hatchery population in Tongyeong, Korea were accessed using multiplex assays with nine highly polymorphic microsatellite loci. A total of 250 different alleles were found over all loci. Despite a long history of hatchery practices, very high levels of polymorphism (mean alleles = 22.89 and mean heterozygosity = 0.92) were detected between the two populations. No statistically significant reductions were found in heterozygosity or allelic diversity in the hatchery population compared with the wild population. However, significant genetic heterogeneity was found between two populations. These results provide no evidence to show that hatchery practice of Pacific oyster in Korea has significantly affected the genetic variability of the hatchery stock. Although further studies are needed for comprehensive determinations of the hatchery and wild populations with increased number of Pacific oyster sample collections, information on the genetic variation and differentiation obtained in this study can be applied for genetic monitoring of aquaculture stocks, genetic improvement by selective breeding and designing of more efficient conservation management guidelines for these valuable genetic materials.

철분 및 Ascorbic acid의 투여가 임산부와 신생아 빈혈에 미치는 영향

안혜숙,조영제,김종덕 대한산부인과학회 1986 대한산부인과학회 학술대회 Vol.58 No.-

Population genetic structure of the tongue sole (Cynoglossus semilaevis) in Korea based on multiplex PCR assays with 12 polymorphic microsatellite markers

안혜숙,강희웅,한현섭,박중연,명정인,안철민 한국유전학회 2014 Genes & Genomics Vol.36 No.4

The tongue sole, Cynoglossus semilaevis, is animportant fishery resource in Korea. About 100 tongue solesampled from three major habitats along the western coast ofKorea were assessed using multiplex assays with 12 highlypolymorphic microsatellite loci to explore the populationgenetic structure of the species; 151 alleles and similar highlevels of gene diversity (mean number of alleles(NA) = 10.42, mean expected heterozygosity (He) = 0.78)were detected. Three populations showed significant Hardy–Weinberg equilibrium deviations at four loci. Although asignificant difference in the number of unique alleles wasobserved among populations, genetic population subdivisionwas low by F-statistics (overall FST = 0.007, p\0.05). However, this substructure was not supported by analysis ofmolecular variance or analyses of isolation by distance. Theresults suggest a lack of genetic structure among the tonguesole populations in Korean waters and that the populationsshould be managed as a single unit. The lack of geneticdifferentiation among samples may be due to high levels oflarval dispersal in ocean currents. Alternatively, the populationsmay have diverged too recently for significant geneticdifferentiation to have become evident. Given the intensityof tongue sole aquaculture activity in China, which adjoinsthe western coast of Korea, the possibility that aquaculturemay have partially contributed to the population geneticcharacteristics detected cannot be excluded. This studyprovides the basic information on nature population structureof C. semilaevis that may help to preserve and manage tonguesoles in Korea.