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Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo
신종봉,박남용,김용환,조호성 한국동물위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.4
In present study, we described the reliability of the dual priming oligonucleotide(DPO) multiplex polymerase chain reaction(PCR) for the detection of Mycobacterium tuberculosis complex(MTC) and non-Mycobacterium tuberculosis(NMT) in blood samples of the Korea native cattle, Hanwoo. Among 340 samples 22(6.5%) were positive in using DPO multiplex PCR, 21(6.2%) were positive in PCR. The relative agreement between 2 tests was 99.7%, and the agreement quotient(kappa), was 0.95(excellent). In these results, we demonstrated the successful application of DPO multiplex PCR for the diagnosis of bovine tuberculosis in Hanwoo. Multiplex PCR, using DPO primers, can be useful for the simple diagnosis of bovine tuberculosis in bovine blood samples.
간세포 성장인자가 HT 세포주에서 matrix metalloproteinase 의 발현에 미치는 영향
신종철(Jong Chul Shin),문희봉(Hee Bong Moon),이지현(Jee Hyun Lee),양동은(Dong Eun Yang),이귀세라(Gui Se Ra Lee),이영(Young Lee),이종승(Jong Seong Lee),김창이(Chang Yi Kim),김수평(Soo Pyung Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.12
N/A Objective : This study was performed to investigate the influence of hepatocyte growth factor (HGF) on matrix metalloproteinase (MMP), which are related in the lysis process of tissue during the invasion of trophoblasts. Method : HT cell line was treated with recombinant HGF (rHGF) of different concentration (0, 10, 50 and 100 ng/mL) and was cultured for 24 hours to check the changes in the expression of MMP-2 and MMP-9. Also, HT cell line was treated with recombinant HGF 50 ng/mL and was cultured for 24, 36, 48, and 72 hours to check the changes in the expression of MMPs according to the different time span. Total RNA were extracted from each cultured sample and RT-PCR and Western blotting were used to analyze the expression of MMP-2 and MMP-9. Results : MMP-2 mRNA expression with treated rHGF showed increase of 2, 2.5 and 2.2 times with the increase of concentration level of 10, 50 and 100 ng/mL accordingly, while MMP-2 protein expression were increased 1.4 and 1.5 times in 50 ng/mL and 100 ng/mL of rHGF respectively compared with that of normal control. MMP-9 mRNA showed no significant changes in its expression with all different levels of concentration, while MMP-9 protein showed 1.5 times increase with 10 ng/mL rHGF but 0.4 times decrease with 100 ng/mL. MMP-2 mRNA expression treated with recombinat HGF were increased 1.6 times with 24 hour culture and 2.3 times with 36 hour culture. MMP-2 protein showed 1.9 times increase only for the case of 24 hour culture. MMP-9 mRNA expression of recombinant HGF-treated groups was decreased 0.7 times compared with that of control group in 36 hours. MMP-9 protein expression were increased by 1.2, 1.6 and 1.9 times as culture time increase to 36, 48, and 72 hours accordingly, compared with that of normal control. Conclusion : This result suggests that the HGF might partially regulate the invasion of trophoblasts through MMP-2 and MMP-9.
간세포 성장인자가 HT 세포주에서 c - met 의 발현과 침투성에 미치는 영향
신종철(Jong Chul Shin),이지현(Jee Hyun Lee),양동은(Dong Eun Yang),오민정(Min Jeong Oh),문희봉(Hee Bong Moon),이영(Young Lee),김사진(Sa Jin Kim),이종승(Chong Seung Yi),김수평(Soo Pyung Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8
N/A Objective : To evaluate the invasive capacity of trophoblastic cells when treated with HGF, and see whether HGF induces c-met expression in vitro. Methods : HT cell line was treated with recombinant HGF (rHGF) at different concentrations (0, 10, 50 and 100 ng/mL) and cultured for 24 hours. To evaluate according different time of culture, HT cell line was pre-treated with 50 ng/mL rHGF and cultured for 24, 36, 48 and 72 hours. We analyzed the expression of c-met mRNA using reverse transcriptase-polymerase chain reaction and the expression of c-met protein using western blot in each samples. We also observed cellular invasion capacity through the invasion assay under a microscope and confirmed 72 kDa gelatinase and 92 kDa gelatinase expression patterns by zymography assay. Results : The expressions of c-met mRNA and protein were increased in all concentrations of rHGF, compared with that of normal control although it was not in dose-dependent fashion. In invasion assay, the number of invaded HT cells were increased in dose-dependent fashion, compared with that of normal control. In zymography ssay, the expression of 72 kDa gelatinase was increased in dose-dependent fashion, compared with the control. However, 92 kDa eglatinase was not detected in any studied group. Conclusion : These results suggests that HGF might be related to upregulation of trophoblast cell invasiveness by activation of c-met and subsequent induction of 72 kDa gelatinase.
Knowledge and Acceptability about Adult Pertussis Immunization in Korean Women of Childbearing Age
고현선,신종철,조윤*,김연희,박용규,위정하,천주영,문희봉,이영 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.4
Purpose: The adult tetanus, reduced diphtheria, and acellular pertussis (Tdap) vaccinehas been introduced in order to provide individual protection and reduce the risk of transmitting pertussis to infants. We assessed the knowledge and acceptabilityof the Tdap vaccine around pregnancy. Materials and Methods: This study was a cross-sectional survey of women of childbearing age (20‒45 years) who visited obstetrics and gynecologic units of primary, secondary, or tertiary hospitals. They were asked to fill in a questionnaire assessing their knowledge, attitudes, and acceptabilityof Tdap. Results: The questionnaire was completed by 308 women; 293 (95.1%) had not received information from doctors about Tdap, and 250 (81.2%) did not know about the need for vaccination. A significantly important factor related to subjects’ intention to be vaccinated, identified by stepwise multiple logistic regression,was the knowledge (OR 13.5, CI 3.92‒46.33) that adult Tdap is effective in preventing pertussis for infants aged 0‒6 months. Additionally, 276 (89.6%) consideredthe recommendation of obstetric doctors as the most influencing factor about Tdap vaccination. Conclusion: In Korea, most women of childbearing age seem to be neither recommended nor adequately informed about the vaccination, although our population was not a nationwide representative sample. Information given by healthcare workers may be critical for improving awareness and preventingpertussis
이동현,신종철,이지현,김수평,문희봉,김사진,이종승 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.9
Chromosomal anomaly is common in twin pregnancies and when it is complicated with discordancy of fetal chromosomal trisomy, optimal management is difficult to decide. Genetic counseling is necessary and delicate management is required to prevent complications like preterm birth of the normal fetus. Although Edward syndrome the second most common trisomy that reaches to live birth after Down syndrome, its loss rate after second trimester reaches to almost 86% resulting in fetal death in utero, and 90% of the few who survive to term die in the first year of life. When twin pregnancy is discordant for Edward syndrome, fetal death in utero of the anomalous baby occurs frequently. One fetal death in twin pregnancy, reaching the third trimester may increase the risk of mortality and morbidity to both surviving fetus and mother. We present a case of twin pregnancy discordant for trisomy 18 that close follow up result in the delivery of a full term healthy boy, although fetal death in utero occurred in trisomy fetus at 34 weeks in gestation with literatures.
전기전도도측정에 의한 유우준임상형 유방염의 진단에 관한 연구 2. 전기전도도치의 변동요인
강병규,신종봉,Kang Byong-Kyu,Shin Chong-Bong 한국임상수의학회 1985 한국임상수의학회지 Vol.2 No.1
To probe the subclinical mastitis in a herd of cows in Chonnam district, the electrical conductivity(EC) of 825 foremilk samples were measured for 2 years. Normal (n=487) and mastitic(n=110) foremilk samples were classified by the California mastitis test (CMT) and direct somatic cell count(DSCC) and investigated the relations between the changes of the EC value and the calving history, age, days of postpartum, estrus and causative organism isolated. Obtained results are summarized as follows. 1. In the normal foremilk samples, positive correlation, though not significant, was found between the EC value and calving history (r=0.573) and age (r=0.247). 2. In the normal foremilk samples, the EC values were lowered at 30~120 days of postpartum through the whole lactation period and revealed a tendencies to higher values following the day of postpartum increased untill to the drying off (r=0.823), and the days of postpartum was recognized as one of a influencing factor on the EC value(p<0.05). 3. In the mastitic foremilk samples, significant correlation between EC value and resazurin reduction test (RRT) were observed (r=0.904, p<0.05). 4. In the mastitic foremilk samples, EC values were obtained in the E. coli infection as 63.9mM-NaCl, in the Streptococcus spp. infection as 60.5mM-NaCl and in the Staphylococcus spp. infection as 57.0mM-NaCl. 5. At day 0 of estrus, the mean EC values of normal and mastitic foremilk samples were 41.2mM-NaCl and 68.3mM-NaCl respectively and the EC value of day 0 of estrus was higher than that of days before and after estrus.
분만 후 첫 번째 번식검진시 난소 및 자궁 질환에 따른 유성분 수준 비교
문진산,신종봉,손창호,주이석,강현미,김종만,Moon, Jin-San,Shin, Chong-Bong,Son, Chang-Ho,Joo, Yi-Seok,Kang, Hyun-Mi,Kim, Jong-Man 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
The relationship between level of milk composition and conditions of ovary and uterus were analyzed in Holstein cows at seven farms participating in a reproductive herd health management program. Milk data were taken from 503 early lactating cows between 30 and 60 days in milk with reproductive examination with ultrasonography from september 1999 to August 2000. Milk fat, protein and solid-not-fat concentration in the herds were $3.70{\pm}1.08%$, $2.97{\pm}0.35$, and $8.41{\pm}0.61%$, respectively. The reproductive disorder relative to normal cows had higher risk in the cows that the level of protein was lower than 2.70%. Also, the higher milk fat than 4.50% were associated with a higher risks in the uterine disease and follicular cysts. Therefore, the cows with the fat to protein ratio of > 1.30 had higher risks for reproductive disorder such as cystic ovarian diseases, inactive ovaries and endometritis. These results indicated that cows diagnosed with reproductive disorder were energy deficient prior to reproductive disorder diagnosis. Consequently, milk fat and protein analyses may be used serve as a monitoring tool for condition of ovary and uterus in early lactating cows