Cyanoacrylate가 成犬 齒周瓣膜의 治癒에 미치는 影響

申載熙,卞種秀,朴準奉 慶北大學校 齒科大學 1986 慶北齒大論文集 Vol.3 No.1

butyl cyanoacrylate가 一般的인 縫合絲에 比해 齒 剝離搔爬術後 齒周辯膜의 治癒에 미치는 影響을 糾明하고자 5마리의 雜種犬 ( 6∼8kg )을 利用, 이들의 上下顎 左右 犬齒에 該當하는 頰側 附着齒 에 齒 剝離搔爬術을 施行하고 上記 2가지 方法을 區分하여 樂劑의 塗布 및 縫合을 施行한 後 定한 날에 實驗動物을 儀牲시켜 組織學的 檢鏡을 통해 이를 觀察하여 다음과 같이 結果를 얻었다. 組織學的 所見에서 初期에는 實驗部가 對照部에 比해 下部組織에 淨腫性 纖維素綱과 炎症細胞浸潤이 微弱하였고, 裂溝上皮細胞의 被服과 纖維性 骨膜의 形成이 迅速하였으며 纖維性 結締組織에 의한 齒周 膜과 齒面과의 癒合도 빨리 일어났다. releasing incision 部位에서도 實驗部가 炎症細胞浸潤이 보다 적었고, 肉茅組織의 形成이 迅速히 일어났으며 그 量도 적었다. 그러나 組織學的 觀察의 全 過程을 통해 3週에서는 治癒程度에 있어서 兩者間의 差異가 없었고, 4週에서도 兩者間의 治癒程道에 대한 差異를 觀察할 수 없었다. This is study was made in order to determine the histological changes associated with healing following the use of cyanoacrylates and silk sutures in canine areas where periodontal flap procedures had been carried out. In this experiment 5 adult mongrel dogs were used. The cyanoacrylate on the right side and black silk suture on the left buccal side of maxilla and mandibular canine area, were applied for the fixation of the flap after the periodontal flap surgery. On the experimental days, the experimental areas were examined clinically and sacrificed. Thereafter histological secimens were made following the useal methods and examined with light microscope. The results were as follows : In the histologic observations, the experimental areas as compared with the controls ; There was weak inflammatory cell infiltration and less edematous fibrin mesh formation at the substructure of the flap. The sulcus epithelial cell covering and formation of the fibrous periosteum was made rapidly. The union of periodontal flap and tooth surface by fibrous connective tissue was also made rapidly at the experimental areas. At the areas of the releasing incision less inflammatory cell infiltration and rapidity and small amount of granulation tissue formation were found. But through the entire procedure of the histologic observations, there was little difference in the degree of healing between the two in the 3rd week and it was also impossible to find the difference of healing between the two in the 4 th week.

Arbekacin의 methicillin 내성 Staphylococcus aureus와 Pseudomonas aeruginosa에 대한 항균력

정윤섭,이경원,신정원,신희봉,임종백 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.3

배경 : MRSA와 P. aeruginosao는 흔한 원내 감염균이고 aminoglycoside를 포함한 여러 항균제에 내성인 균주가 많다. Arbekacin은 aminoglycoside제로서 일본에서는 MRSA 감염을 치료를 위해 사용되고 있다. 이 연구에서는 MRSA가 확산되기 시작하던 시기와 근래에 분리된 MRSA에 대한, 그리고 내성양상에 따라서 선택된 P. aeruginosa에 대한 arbekacin의 항균력을 비교하고자 하였다. 방법 : 균주는 세브란스병원에서 분리하였다. P. aeruginosa 균주는 amikacin, ceftazidimie, imipenem 및 ciprofloxacin에 대해 감수성 혹은 내성인 것을 선택하였다. 감수성은 NCCLS 디스크 확산법 및 한천희석법으로 시험하였다. 결과 : Arbekacin≤14㎍/mL는 모든 MRSA 균주를 억제하였다. 1980년과 1996년에 분리한 MRSA에 대한 arbekacin의 MIC_(90)은 2㎍/mL로 같았고, MSSA에 대해서는 0.5 ㎍/mL이었다. MRSA가 높은 내성율을 보인 amicoglycoside부터 나열하면 kanamycin, gentamicin, tobramycin, amikacin, isepamicin의 순이었다. Amikacin 감수성인 P. aeruginosa에 대한 arbekacin의 MIC??은 amikacin 보다 약간 높았으나, ceftazidime, impenem 및 ciprofloxacin에 감수성 혹은 내성인 균주에 대해서 보다는 약간 낮았다. Arbekacin 감수성 균주의 비율은 다른 항균제에 대한 감수성이나 균주의 비율은 다른 항균제에 대한 감수성이나 내성인 균주 군에 따라서 약 1/3 내지 2/3이었다. 결론 : 본 시험관내 감수성 성적으로 볼 때, arbekacin은 MRSA 감염의 치료와, 일부 MRSA와 P. aeruginosa의 혼합감염 치료에 유효할 것으로 사료되었다. Background : Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa are very prevalent nosocomial pathogens, often resistant to multiple drugs including various aminoglycosides. Arbekacin is an aminoglycoside used for the treatment of MRSA infections in Japan. The aim of study was to compare the activities of arbekacin against the MRSAs isolated currently and at the start of the spread, and against the selected isolates of P. aeruginosa susceptible or resistant to amikacin, ceftazidime, impenem and ciprofloxacin. Methods : The strains were isolated from Severance Hospital. The NCCLS disk diffusion and agar dilution tests were used and strains inhibited by 4 ㎍/mL of arbekacin was interpreted as susceptible and 16 ㎍/mL resistant. Results : Arbekacm ≤4 ㎍/mL inhibited all of the MRSAs tested. The MiC_(90)s for MRS4s isolated in 1980 and 1996 were equal; 2 ㎍/mL, while that for MSSA was 0.5 ㎍/mL. All of the MRSA were susceptible. The resistance rates. in descending order, were to kanamycin, gentamicin, tobramj-cin, amikacin and isepamicin. MIC,, of arbekacin for amikacin-susceptible P. aerugznosa m-as slightly higher than that of amikacin, but slightly lower than those for the strains both susceptible and resistant to ceftazidxme, imipenem and ciprofloxacin. Depending on the susceptibility groups of strains, approximate15 1/3 to 2/3 were susceptible to arbekacin. Conclusion : Arbekacin may be useful for the treatment of single MRSA infection and for some of the mixed infections of MRSA and P. aerugznosa.

Comparison of the Gut Microbiota of Centenarians in Longevity Villages of South Korea with Those of Other Age Groups

( Bong-soo Kim ),( Chong Won Choi ),( Hyoseung Shin ),( Seon-pil Jin ),( Jung-soo Bae ),( Mira Han ),( Eun Young Seo ),( Jongsik Chun ),( Jin Ho Chung ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3

Several studies have attempted to identify factors associated with longevity and maintenance of health in centenarians. In this study, we analyzed and compared the gut microbiota of centenarians in longevity villages with the elderly and adults in the same region and urbanized towns. Fecal samples were collected from centenarians, elderly, and young adults in longevity villages, and the gut microbiota sequences of elderly and young adults in urbanized towns of Korea were obtained from public databases. The relative abundance of Firmicutes was found to be considerably higher in subjects from longevity villages than those from urbanized towns, whereas Bacteroidetes was lower. Age-related rearrangement of gut microbiota was observed in centenarians, such as reduced proportions of Faecalibacterium and Prevotella, and increased proportion of Escherichia, along with higher abundances of Akkermansia, Clostridium, Collinsella, and uncultured Christensenellaceae. Gut microbiota of centenarians in rehabilitation hospitals were also different to those residing at home. These differences could be due to differences in diet patterns and living environments. In addition, phosphatidylinositol signaling system, glycosphingolipid biosynthesis, and various types of N-glycan biosynthesis were predicted to be higher in the gut microbiota of centenarians (corrected p < 0.05). These three metabolic pathways of gut microbiota can be associated with the immune status and healthy gut environment of centenarians. Although further studies are necessary to validate the function of microbiota between groups, this study provides valuable information on centenarians’ gut microbiota.

Cefminox의 Enterobacteriaceae 균종, Haemophilus influenzae 및 Methicillin 감수성 Staphylococcus aureus에 대한 시험관내 항균력

정윤섭,이경원,신희봉,정재림 대한감염학회 1998 감염 Vol.30 No.5

목적: 근래 분리되는 Enterobacteriaceae 중에는 ESBL 생성균이 드물지 않으며, H. influenzae 중에는 β-lactamase 생성균이 많고, MSSA는 대부분이 penicillin G에 내성이다. Cefminox는 흔히 사용되는 cephamycin이지만 근래 분리되는 이들 균종에 대한 항균력이 시험된 바 없으므로 그 항균력을 시험하고 다른 항균제의 항균력과 비교하고자 하였다. 방법: 시험세균은 1997-1998년에 세브란스병원 환자에서 분리하였다. 항균제 감수성은 NCCLS 한천희석법으로 시험하였다. 방법: E. coli는 모든 균주가, K. pneumoniae는 90%가 cefminox, ceftetan 및 amikacin에 감수성이었다. E. cloacae, S. marcescens는 모든 균주가, C. freundii는 86%가 amikacin에 감수성이었다. P. mirabilis, P. vulgaris 및 M. morganii는 모든 균주가 cefminox, cefotetan cefotaxime, cefazidime 및 aztreonam에 감수성이었고, Providencia spp.는 모두가 ceftazidime 과 aztreonam에 감수성이었다. H. influenzae는 모두 균주가 cefminox, cefotaxime 및 levofloxacin에 감수성이었고, MSSA는 모두가 cefoxitin이나 cotrimoxazole에 감수성이었다. 결론: 10% 이하의 균주가 내성을 보인 Enterobacteriaceae 균종수로 볼 때 cefminox와 aztreonam의 항균력이 가장 강하며, 특히 ESBL 생성 세균의 증가에 따라서 cephamycin계 항균제의 상대적인 유용성은 더 커졌으며, E. colacae, S. marcescens 및 C. freundii는 β-lactam 항균제에 대해서 보다 amikacin이나 levofloxacin에 대한 감수성율이 더높으며, H. influenzae 중에는 β-lactamase 생성균이 흔해서, ampicicillin/sulbactam, 제3세대 cephalosporin 또는 levofloxacin에 대한 감수성율이 ampicillin에 대해서 보다 현저히 높으며, MSSA는 대부분이 penicillinase를 생성하지만 β-lactamase에 안정한 penicillin이나 cephalosporin 뿐 아니라 levofloxacin이나 clindamycin에도 대부분의 균주가 감수성이라는 결론을 얻었다. Background: Recent clinical isolates of bacteria often produce various β-lactamases, for example, extended spectrum β-lactamase (ESBL) by some species of Enterobacteriaceae, TEM β-lactamase by Haemophilus influenzae, and penicillinase by methicillin-susceptible Staphylococcus aureus (MSSA). Cefminox, a commonly used cephamycin, is stable to various β-lactamases, but its activity against recent clinical isolates has not been evaluated. The aim of this study was to determine the activities of cefminox against recent clinical isolates of Enterobacteriaceae, H. influenzae, and MSSA. Methods: The organisms, isolated from Severence Hospital patients during 1997 to 1998, were kept frozen until the test. Antimicrobial susceptibility was determined by the NCCLS agar dilution method. Results: All 30 isolates of Escherichia coli and 90% of 30 Klebsiella pneumoniae isolates were susceptible to cefminox, cefotetan and amikacin. All of the Enterobacter cloacae and Serratia marcescens, and 86% of Citrobacter freundii isolates were susceptible to amikacin All of the 15 isolates each of Proteus mirabilis, P. vulgaris and Morganella morganii were susceptible to cefminox, cefotetan, cefotaxime, ceftazidime, and aztreonam, while all of the Probidencia spp. were susceptible to ceftazidime and aztreonam. All of the 29 H. influenzae isolates were susceptible to cefminox, cefotaxime, and levofloxacin, while all MSSA isolates were susceptible to cefoxitin and cotrimoxazole. Couclusion: Cefminox is more active than the other β-lactams against Enterobacteriaceae including, ESBL-producing E. coli, and K. pneumoniae.

Expression system for production of bioactive compounds, recombinant human adiponectin, in the silk glands of transgenic silkworms

Shin, Seulmee,Kim, Bong-Yoon,Jeon, Hyung-Yook,Lee, Aeri,Lee, Sungwon,Sung, Seung-Hyun,Park, Chan-Su,Lee, Chong-Kil,Kong, Hyunseok,Song, Youngcheon,Kim, Kyungjae 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

Adiponectin is an adipocyte hormone involved in glucose and lipid metabolism. The aim of this study was to develop a human adiponectin expression system in transgenic silkworm using a human adiponectin expression vector. The silk gland of the silkworm is a highly specialized organ that has the wonderful ability to synthesize and secrete silk protein. To express human adiponectin in the silk gland of transgenic silkworm, targeting vectors pB-A3-adiponectin-IRES-RFP and pB-Ser1-adiponectin-IRES-RFP were constructed and then introduced into the silkworm pupa. The transgenic silkworms were verified by PCR and then generated. The level of adiponectin in the transgenic silkworm was 6-10 ng/50 mg of freeze-dried powder, and western blotting using an antibody against human adiponectin demonstrated a specific band with a molecular weight of 30 kDa in the silkworm. These results showed that human adiponectin introduced into the silkworm genome was expressed successfully on a large-scale.

Bandgap modulations in monolayer MoS₂ by substrate-induced strain

Bong Gyu SHIN,Gang Hee HAN,Seok Joon YUN,Hye Min OH,Jung Jun BAE,Young Jae SONG,Chong-Yun PARK,Young Hee LEE 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Self-Assembled Nanostructure of Block Copolymers on Random Copolymer Brush

Bong Hoon Kim,Chong Min Koo,Seong Jun Jeong,Dong Ok Shin,Sang Ouk Kim 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.10

The Synthesis of Random Brush for Nanostructure of Block Copolymer

Kim, Bong Hoon,Koo, Chong Min,Shin, Dong Ok,Jeong, Seong Jun,Kim, Sang Ouk WILEY-VCH Verlag 2007 Macromolecular symposia Vol.249 No.1

<P>Polymer brush has been synthesized via conventional free radical polymerization to use a surface active material on the silicon substrate. The synthesized polymer brushes were successfully grafted onto the silicon oxide substrate and the hydrophilic silicon oxide substrate has been changed into the more hydrophobic one. The synthesized poly(styrene-ran-methyl methacrylate) brush grafted silicon substrate could provide poly(styrene-block-methyl methacrylate) with neutral surface.</P>

Anomalous Rapid Defect Annihilation in Self-Assembled Nanopatterns by Defect Melting

Kim, Bong Hoon,Park, So Jung,Jin, Hyeong Min,Kim, Ju Young,Son, Seung-Woo,Kim, Myung-Hyun,Koo, Chong Min,Shin, Jonghwa,Kim, Jaeup U.,Kim, Sang Ouk American Chemical Society 2015 NANO LETTERS Vol.15 No.2

<P>Molecular self-assembly commonly suffers from dense structural defect formation. Spontaneous defect annihilation in block copolymer (BCP) self-assembly is particularly retarded due to significant energy barrier for polymer chain diffusion and structural reorganization. Here we present localized defect melting induced by blending short neutral random copolymer chain as an unusual method to promote the defect annihilation in BCP self-assembled nanopatterns. Chemically neutral short random copolymer chains blended with BCPs are specifically localized and induce local disordered states at structural defect sites in the self-assembled nanopatterns. Such localized “defect melting” relieves the energy penalty for polymer diffusion and morphology reorganization such that spontaneous defect annihilation by mutual coupling is anomalously accelerated upon thermal annealing. Interestingly, neutral random copolymer chain blending also causes morphology-healing self-assembly behavior that can generate large-area highly ordered 10 nm scale nanopattern even upon poorly defined defective prepatterns. Underlying mechanisms of the unusual experimental findings are thoroughly investigated by three-dimensional self-consistent field theory calculation.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2015/nalefd.2015.15.issue-2/nl5042935/production/images/medium/nl-2014-042935_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl5042935'>ACS Electronic Supporting Info</A></P>

Prevalence, Antibiotic Resistance, and Virulence Features of Shiga Toxin-producing Escherichia coli Isolates from the Retail Meats and Meat by-products in Korea

June Bong Lee,Dalmuri Han,Jeong Hoon Park,Hyung Tae Lee,Hyun Seok Joh,Yeon Soo Chung,Suk Shin,Yong Ho Park,Chong Hae Hong,Jang Won Yoon 대한수의학회 2015 대한수의학회 학술대회발표집 Vol.2015 No.-