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중합효소 연쇄반응법 ( Polymerase Chain Reaction ) 을 이용한 Helicobacter pylori 의 검출
이준성(Joon Seong Lee),송동화(Dong Wha Song),박찬욱(Chan Wook Park),이문성(Moon Sung Lee),조성원(Sung Won Cho),심찬섭(Chan Sup Shim),진소영(So Young Jin) 대한내과학회 1995 대한내과학회지 Vol.49 No.3
N/A Objectives: Helicobacter pylri (H. pylori) infection is now known to be the major cause of chronic gastritis, and is strongly associated with peptic ulcer disease and gastric cancer. But no unanimity exists among investigators about which method represents an appropriate gold standard for diagnosing H. pylori infection. Polymerase chain reaction(PCR) has great potential for improving the ability to diagnose infectious diseases caused by fastidious or slow growing bacterias. But its clinical usefulness as a diagnostic tool for H. pylori is uncertain. Methods: We performed endoscopic biopsy of stomach (>3 pieces, at antrum) for the rapid urease test (CLO stain) and PCR in 11 patients with duodenal ulcer, 2 patients with gastric ulcer, 3 patients with combined gastric and duodenal ulcer, 2 patients with gastric cancer, and 12 patients with gastritis. In preliminary study, endoscopic instruments were tested for residual H. pylori using PCR after combined manual and machine-cleaning and ultrasonic washing with disinfection in 5 patients with positive CLO test. Gastric biopsy tissues were digested in proteinase K(F.C.: 500pl/ml) for DNA extraction, and PCR amplification was performed by using 20 base oligonucleotide primers(CAM 2, CAM 4) homologous to a portion of the 1.9-kb fragment. PCR assay amplified a 203 bp product which was analyzed by agarose gel electrophoresis. Results: 1) in preliminary study, H. pylori were detected by PCR in 4 postendoscopic samples of working channels and biopsy forceps of 2 patients, and 1 preendoscopic sample of biopsy forceps, 2) Of the 30 patients, 19(63.3%) were positive for H. pylori by PCR, 17(56.7%) were positive by CLO test and silver staining. 3) The grade of severity of gastritis on H-E stain was well correlated with the grade of H. pylori infection on Warthin-Starrry silver stain(r=0.651, p<0.01). 4) Discordant results between PCR & other diagnostic tools were 3 cases. Repeat PCR tests disclosed the same results. Conclusion: PCR test of H. pylori is the most sensitive and reproducible test and can overcome the disadvantages of other diagnostic techniques showing less sensitivity and specificity, but it must be dealt with caution of instrumental contamination.
기경도 ( Kyung Do Ki ),변재용 ( Jae Yong Byun ),김상훈 ( Sang Hoon Kim ),송동화 ( Dong Wha Song ),권기환 ( Kee Hwan Kwon ),박문서 ( Moon Suh Park ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.4
목적: 임신 시 벨마비의 발생 빈도는 비임신 시에 비해 높게 보고되고 있다. 비임신 시와 비교하여 임신 시 발생한 벨마비의 발병 시기, 임상 양상, 회복률에 대해 알아보고자 하였다. 연구 방법: 1996년부터 2006년까지 임신 중 벨마비가 발생한 43명의 환자를 대상으로 하였으며 비임신 시 벨마비가 발생한 가임기 여성 61명을 대조군으로 의무기록을 토대로 후향적 연구를 시행하였다. 결과: 임신 중 벨마비는 평균 재태연령 32주에 발생하였으며, 81.4% Objective: Although Bell`s palsy is not common in pregnancy, it is more prevalent among pregnant women than among non-pregnant women. Since the exact clinical characteristics of this condition are not fully understood, we evaluated the epidemiology, pathop
원저 : 농흉환자에서의 늑막강내 유로키나제주입 전후의 섬유소 용해에 관한 연구
김용훈 ( Yong Hoon Kim ),김종봉 ( Jong Bong Kim ),문종호 ( Jong Ho Moon ),송동화 ( Dong Wha Song ),김현태 ( Hyeon Tae Kim ),양동호 ( Dong Ho Yang ),이상무 ( Sang Moo Lee ),어수택 ( Soo Taek Uh ),박춘식 ( Choon Sik Park ) 대한결핵 및 호흡기학회 1993 Tuberculosis and Respiratory Diseases Vol.40 No.4
Interferon-γ와 Granulocyte Macrophage-Colony Stimulating Factor가 복강대식 세포의 기능에 미치는 영향
이지윤,우준희,이희발,한동철,송동화,황승덕,이상구 대한감염학회 1994 감염 Vol.26 No.1
배경: CAPD환자에게 복막염을 유발하는 요소 가운데 복강내 국소 방어기전을 담당하는 복강대식세포의 기능 저하가 중요한 역할을 하고 있다. IFN-γ와 GM-CSF는 시험관 또는 동물실험을 통해 대식세포 기능 활성화를 조절하는 것이 알려졌다. 저자들은 CAPD 환자에서 저하된 복강대식세포 기능을 IFN-γ와 GM-CSF로 증강시킬 수 있는지 알기 위하여 본 연구를 시행하였다. 방법: 11명의 CAPD 환자에서 10⁴U/ml의 IFN-γ와 40㎍/ml의 GM-CSF를, 분리된 1×10 6/ml의 복강대식 세포와 같이 배양하고 유량세포측정법(flow cytometry)을 이용하여 HLA-DR 표현율, FcγRⅢ 표현율, FITC 표지 포도구균을 이용한 탐식 기능의 배양전 결과와 배양후 결과를 비교하여 다음과 같은 성적을 얻었다. 결과 1) 복강대식 세포의 HLA-DR 표현율은 IFN-γ나 GM-CSF와 배양전후에 유의한 차이는 없었으나 GM-CSF 배양후 증가되는 경향을 보였다. 2) 복강대식 세포에서 FcγRⅢ 표현율은 IFN-γ나 GM-CSF와 배양전 후에 유의한 차이는 없었으나, IFN-γ와 배양후 증가되는 경향을 보였다. 3) FITC 표지 포도구균을 이용한 복강대식 세포의 탐식기능은 IFN-γ와 배양한 뒤 의미있게 증가되었고 (P<0.05), GM-CSF와 배양한 뒤 증가경향이 관찰되었다. (P=0.07). 결론 : CAPD 환자의 복강대식세포는 IFN-γ와 GM-CSF에 의하여 HLA-DR 표현율, FcγRⅢ 표현율과 탐식기능의 증가하는 것으로 보아 IFN-γ와 GM-CSF를 복강내로 투여하여 복강대식세표의 기능을 활성화 시킴으로써 복박염 발생빈도를 감소시킬 수 있을 것으로 생각되었다. Background ; Peritonitis remains the most common cause of CAPD drop out. Reduced activity of peritoneal macrophages (M) may be one of the risk factors for peritonitis during CAPD. Interferon-γ (IFN-γ) and Granulocyte-macrophage Colony Stimulating Factor (GM-CSF) have been shown to control M activity in animal and in vitro experiments. This study was designed to evaluate the effect of IFN-γ and GM-CSF on peritoneal M during CAPD. Methods :M were separated from overnight peritoneal effluent from eleven CAPD patients using modified Ficoll-Hypaque methods. The 1 x 10 6/ml of peritoneal M were incubated with 10⁴units/ml of IFN-γ or 40㎍/ml of GM-CSF. Peritoneal M HLA-DR expression, IgG FcⅢ receptor(FcγRⅢ) expression, and bacterial phagocytosis were measured before and after incubation with IFN-γ or GM-CSF by two color flow cytometry using monoclonal antibodies. Results : 1. HLA-DR expression did not change after IFN-γ but increased after GM-CSF, although statistically insignificant. 2. M FcγRⅢ expression did not change after GM-CSF but increased after IFN-γ, although statistically insignificant. 3. Bacterial phagocytosis utilizing FITC labeled S. aureus increased after IFN-γ (P<0.05) and increased also after GM-CSF, although statistically insignificant. Conclusion : IFN-γ and GM-CSF augmented the peritoneal macorphage activity as shown by significant increase in bacterial phagocytosis and by tendency to enhanced expression of HLA-DR, and FcγRⅢ expression.
악성 식도협착에서의 금속제 확장형 EsophaCoilTM 인공식도삽관술
박찬욱,이준성,오종훈,이문성,조성원,이권호,심찬섭,송동화,유창범 대한소화기내시경학회 1995 Clinical Endoscopy Vol.15 No.1
Endoscopic endoprosthesis is well established as a cheap, fast and durable procedure for palliation of malignant dysphagia. But the placement of conventional endoprosthesis is difficult and is associated with significant complications such as hemorrhage, perforation and dysfunction of the prosthesis. Recently, self -expand- able metal prosthesis have also been utilized for malignant esophageal stenosis, and palliation of this modality seems to be more effective than other modalities. However the main problems with these metal stents are tumor ingrowth leading to reobstruction, migration, and eophageal trauma by the distal, hard skirt of the stent. EsophaCoil stent is a simple coil with close loops made from a single flat wire of nickel titanium alloy. The radial force of this material is much stronger than stainless steel, expansion time is faster and the stent is able to dilate even extremely resistant strictures. This new metalic stent seems to have several advantages over the current commnerically available ones. We report our experiences with this EsophaCoil stent and review of literature.
박찬욱,이준성,이문성,조성원,심찬섭,주재학,이영홍,송동화,류권호 대한소화기내시경학회 1994 Clinical Endoscopy Vol.14 No.4
Trichuriasis is an intestinal infection of human beings caused by Trichuris trichiura, more commonly known as whipworm because of its whip-like appearance. It is characterized by the invasion of the colonic mucosa by the adult trichuris. It is prevalent throughout the world, especially in tropical areas. Diagnosis is made typically by the identification of characteristic barrel-shaped eggs in the feces, although adult worms may be seen rarely at sigmoidoscopy or colonoscopy. We report five cases of whipworm infection that was diagnosed on colonoscopic examination.
경피경간 담낭배액술 및 경피적 복막 배액술로 치유된 담낭천공 1예
박찬욱,이준성,이문성,조성원,심찬섭,송동화,권강호,홍수진 대한소화기내시경학회 1994 Clinical Endoscopy Vol.14 No.4
Perforation of gallbladder is a serious complication of acute cholecystitis with alarmingly high mortality rate. These high mortality and morbidity rates were caused by delay in prompt diagnosis and adequate therapy. Especially, mortality and morbidity rates rise markedly in the elderly patient with severe systemic illness. In the patients of gallbladder perforation who are poor candidate for general anesthesia and major operation, percutaneous cholecystic drainage procedure is good alternatives. We experienced a case of gallbladder perforation which was treated successfully by non-operative percutaneous transhepatic cholecystic drainage(PTCCD) in 65-year-old female. She couldn't be a candidate for cholecystectomy or operative chlecystostomy because of severe adhesion of gallbladder to adjacent organ and tissue due to previous gallbladder empyema. We decided to take non-operative percutaneous transhepatic cholecystic drainage and percutaneous peritoneal drainage of abdominal abscess. Thereafter, we examined gallbladder by percutaneous transhepatic cholecystoscopylPTCCS) and rule out gallstone and gallbladder malignancy. So, we presented the case with the brief review of the literatures.
박찬욱,문철,이동화,이준성,이문성,조성원,심찬섭,진소영,송동화,권강호,홍수진 대한소화기내시경학회 1994 Clinical Endoscopy Vol.14 No.4
Gastational choriocarcinoma is a malignant neoplasm of trophoblast. It may occur after hydatidiform mole, spontaneous abortion, normal pregnancy, and even an ectopic pregnancy. Extragenital choriocarcinoma is a rare tumor which attracts interest because of its controversial pathogenesis. It has been reported to occur within the lung, mediastinum, breast, prostate, thymus, pineal, nose, liver, bladder, and biliary tree, as well as most parts of the gastrointestinal tract. We experienced a case of primary choriocarcinoma of stomach with a metastasis to the liver of a 54-year-old man. So, we present a case with a review of literature.