비타민 B1 유도체(Thiamine Di-lauryl Sulfate:TDS)의 나노입자화를 통한 고추탄저병균의 항진균 활성 증진

서용창,조정섭,정해윤,임태빈,조경숙,이태우,정명훈,이강형,김성일,윤원병,이현용 韓國藥用作物學會 2011 한국약용작물학회지 Vol.19 No.3

This study was performed to enhance antifungal activity of anthracnose in chili pepper by nanopaticles of thiamine di-lauryl sulfate (TDS) through high pressure homogenization process. Yield of TDS was 79.14% by reaction of thiamine hydrochloride and sodium lauryl sulfate. TDS nanopaticle solution was manufactured through high pressure homogenization process. The turbidity of nanoparticles solution was increased with increasing the concentration of TDS, and nanoparticles solution of 100 ppm was showed the highest turbidity with absorbance of 3.212. The size of nanoparticles solution was measured as average 258.6 nm by DLS. Nanoparticles solution of 100 ppm showed growth inhibition activity with higher than about 80% compared to the control group against Colletotrichum gloeosporioides. Finally, nanoparticles solution was increased effectively the penetration of the TDS nanopaticles on attached cell membrane of hyphae and started to destruct the cells under microscope observation. Consequently, we suggested that the TDS nanoparticle solution by high pressure homogenization process might be suitable biochemical pesticides for improving the antifungal activities against anthracnose in pepper.

비타민 B1 유도체 Thiamine Dilauryl Sulfate의 나노 입자 제조를 통한 수용액의 용해도에 따른 항진균 활성 평가

서용창,최운용,이춘근,조정섭,임태빈,정명훈,김성일,윤원병,이현용 韓國藥用作物學會 2011 한국약용작물학회지 Vol.19 No.6

Conventional Thiamine Dilauryl Sulfate (TDS) powder has a low stability. In order to solve this problem, this study was performed to improve the solubility of TDS. The process for enhance solubility of TDS was nano grinding mill and ultrasonic dispersion process. TDS paticle was manufactured to nano size through nano grinding mill process. The size of TDS nanoparticle was measured as average 220 nm by DLS. And The TDS nanoparticle in water solution manufactured through ultrasonic dispersion process. The TDS nanoparticle in water solution was showed the highest solubility with 40% ethanol. These results was increased the concentration of TDS from 200 ppm to 240 ppm in water solution. The TDS nanoparticle in water solution showed diameter of Colletotrichum gloeosporioides growth with smaller than about 1.56 cm compared to the TDS paticle in water solution at same concentration. Also, TDS nanoparticle in water solution showed growth inhibition activity as 59.2% with higher than about 10% compared to the TDS paticle water solution in same concentration. Finally, TDS nanoparticle in water solution was increased solubility through nano grinding mill and ultrasonic dispersion process. Also, the increase of concentration in TDS nanopaticle in water solution according to solubility enhancement lead to an result enhancement of antifungal activity. Consequently, we suggested that the TDS nanoparticle in water solution was more effective than TDS particle in water solution owing to the sub-cellular particle size, ability to persistence and targeting to cell membrane of Colletotrichum gloeosporioides. Furthermore we expected the applicating possibility with bio pesticide.

고로쇠 나무의 수피와 수액의 향장활성 비교

서용창,김지선,최운용,조정섭,임혜원,윤창순,마충제,이현용 韓國藥用作物學會 2011 한국약용작물학회지 Vol.19 No.4

In this study, we investigated the cosmetic application of Acer mono sap through an ultra-high pressure process. Exposing Acer mono sap to a ultra-high pressure process resulted in 90.1% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration. Acer mono sap also showed the hightest free radical scavenging activity after the ultra high pressure process. The melanogenesis inhibition rate in cloned M-3 cells was 59.0%. Tyrosinase was inhibited at a rate of 87.2% by adding 100% HPAMS. Anti-wrinkle activity was 78.1%. Acer mono sap showed enhanced storage following the ultra high pressure process. These results indicate that Acer mono sap may be a source for functional cosmetic agents capable of improving antioxidant, whitening, and antiwrinkling effects.

초고압 가공 공정을 통한 지치 추출물의 항암 활성 증진

서용창,최운용,김지선,조정섭,김영옥,김진철,이현용 한국약용작물학회 2011 한국약용작물학회지 Vol.19 No.2

This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts (1.0μg/ml) from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding 1.0μg/ml of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.

극한 저온 추출 공정을 처리한 지치의 면역활성

서용창,김지선,김영옥,김진철,이현용 한국약용작물학회 2013 한국약용작물학회지 Vol.21 No.2

This study was performed to investigate the enhancement of immunomodulatory activities of Lithospermum erythrorhizon by extreme process. The extracts are WE100 (water extract for 24 hours at 100℃), WE80 (water extract for 24 hours at 80℃), EE (70% ethyl alcohol extract for 24 hours at 80℃) and EPE (extreme process for 30 minutes at 25℃, 500 MPa after 70% ethyl alcohol extracts for 3 hours at 40, 50, 60℃). Extraction yield was increased up to 5~10% by extreme process, compare to the normal extraction such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts was showed in the range of 12.68~15.89% at 1.0mg/ml for human lung cell (HEL299). The EPE40 was showed the lowest cytotoxicity 12.68%. The EPE60 extracted by extreme process increased the growth of human B and T cells up to 12.12×104 cells/ml and 14.88×104 cells/ml, respectively and the EPE60 greatly increased the cytokine secretion of both IL-6 and TNF-α. The extracts by extreme process also exhibited higher levels of nitric oxide production from macrophages than the lipopolysaccaharides. It can be concluded that Lithospermum erythrorhizon has immune activities and The extreme process could increase higher immune activities possibly by immunomodulatory compounds.

고추냉이 수용성 추출물의 나노 입자화를 통한 식중독 미생물에 대한 항균 활성 증진

서용창,최운용,김지선,조윤윤,이춘근,안주희,신일식,이현용 韓國藥用作物學會 2010 한국약용작물학회지 Vol.18 No.6

This work was to improve antimicrobial activities of horseradish by encapsulated with edible biopolymers such as lecithin and gelatin since it has been difficult to directly use horseradish extracts into foods and food containers due to its strong and undesirable flavors. It was shown that most of the nanoparticles containing the extracts were well formed in round shape with below 400 nm diameter as well as fairly stable and less odd flavors in various pH ranges by measuring zeta potentials. The encapsulation efficiencies of nanoparticles were estimated as 66.6% and 53.4% for lecithin and gelatin, respectively. Minimal Inhibitory Concentration (MIC) of both nanoparticles against G(+), Listeria monocytogenes and G(-), Salmonella typhimurium were also measured as 79 ppm based on AIT concentrations in the extracts, whose activities were about 65% higher than the case of adding crude extract. It was also found that the nanoparticles efficiently penetrated into the cell membrane and started to destruct the cells after 6 hours cultivation under Transmision Electron Microscopy observation. These results prove that the nano-encapsulation of the horseradish extracts can be employed to directly treat into the foods and food containers for antimicrobial purposes with the aids of aerosolization system, by using small amounts of the extracts and having less flavors due to masking effects of nanoparticles.

항균 효능이 있는 비타민 B1 유도체(Thiamine Dilauryl Sulfate:TDS)의 나노 입자화를 통한 기능성 향장 활성 증진

서용창 ( Yong Chang Seo ),노라환 ( Ra Hwan No ),권희석 ( Hee-seok Kwon ),이현용 ( Hyeon Yong Lee ) 대한화장품학회 2013 대한화장품학회지 Vol.39 No.3

본 연구는 레시틴으로 나노입자화 시킨 티아민 디라우릴 설페이트의 향장활성 증진에 관한 것이다. TDS를 포집시킨 나노입자는 150 ~ 200 nm의 크기를 나타내는 구형이며, 또한 제타포텐셜을 측정하여 여러 pH 범위에서 안정한 것을 확인하였다. TDS 나노입자는 인간 섬유아세포(CCD-986sk)에 높은 농도를 처리하여도 85%의 세포생존률을 보였다. 자유라디칼소거활성 실험을 진행한 결과 나노입자화하지 않은 TDS 희석액(1.0 mg/mL)은 81.6%의 활성을 나타내었고, 나노입자화한 TDS 용액은 이보다 더 높은 88.1%의 높은 라디칼 소거활성을 보였다. TDS 나노입자는 자외선을 조사시킨 CCD-986sk에서 MMP-1의 발현을 41.4% 감소시켰다. TDS 용액과 TDS 나노입자를 가지고 salmonella typhimurium, listeria monocytogenes에 대하여 항균활성을 측정하였다. TDS 나노입자의 경우 양성대조군의 항균활성과 비슷한 결과를 나타내었다. 이러한 결과들로 TDS 나노입자가 항산화, 미백, 주름개선 효능같은 향장 소재로서의 적용이 가능할 것이라 생각된다. This study was to improve cosmetical activity of thiamine di-lauryl sulfate (TDS) by encapsulation of nanoparticle with lecithin. Results showed that most of the nanoparticles containing the TDS were well formed in round shape with below 150 ∼ 200 nm diameter as well as they were fairly stable in various pH ranges by measuring zeta potentials. The nanoparticles of TDS resulted in 85% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration (1.0 mg/mL). The nanoparticles of Acer mono sap showed highest free radical scavengering effect as 88.1% in adding sample (1.0 mg/mL), compared to TDS solution of non-encapsulation (81.6%). The nanoparticles of TDS reduced the expression of MMP-1 on UV-irradiated CCD-986sk cells down to as 41.4%. The TDS solution and nanoparticles showed significant anti-microbial activities agaionst the salmonella typhimurium and listeria monocytogenes at 5 and 6 days as compared with control. Anti-microbial activities of TDS nanoparticles were similar to positive control. These results indicated that TDS nanoparticles may be a source for functional cosmetic agents capable of improving cosmetical activity such as antioxidant, whitening, and anti-wrinkling effects and can be further developed as natural preservative in cosmetics.

레시틴으로 포집된 마치현 나노입자의 향장 활성

서용창(Yong Chang Seo),최운용(Woon Yong Choi),김지선(Ji Seon Kim),이춘근(Choon Geun Lee),송치호(Chi Ho Song),김영옥(Young Ock Kim),차선우(Seon Woo Cha),이현용(Hyeon Yong Lee) 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.2

무선통신을 위한 나무구조 벡터양자화를 이용한 동영상 부호화기법

서용창(Yong Chang Seo),송준석(Jun Seok Song),이승준(Seung Jun Lee),김철우(Chul Woo Kim),이충웅(Choong Woong Lee) 대한전자공학회 1996 대한전자공학회 학술대회 Vol.1996 No.11

순환형 감압건조에 의한 건조 김치의 품질 특성

서용창 ( Yong Chang Seo ),최영범 ( Young Beom Choi ),고정림 ( Jung Rim Ko ),이현용 ( Hyeon Yong Lee ) 한국산업식품공학회 2013 산업 식품공학 Vol.17 No.4

본 연구는 순환형 감압 건조 장치를 제작하여 이를 통해 건조한 김치의 품질 변화를 연구하였다. 종래 건조 기술인 열풍 건조 방법은 60oC 이상의 열풍에 노출시키는 방법으로 40oC 이상의 높은 온도로 높아지면 식품에 함유되어 있는 성분들이 파괴되거나 변형되어 품질이 저하되고, 외형사의 변형을 일으켜 소비자들에게 거부감을 가질 수 있다. 동결 건조 방법은 -50oC 이하의 온도와 진공 상태에서 건조하는 방법으로 열풍 건조의 문제점인 식품의 변형 및 영양소의 파괴를 막을 수 있지만, 24시간 이상의 건조 시간 및 높은 비용으로 생산 효율이 나쁘다는 문제가 있다. 이러한 문제점을 해결하기 위하여 순환형 감압 건조 장치를 제작하여 본 연구에 적용하였다. 건조 시간 별 함수율에서는 순환형 감압 건조의 건조 속도가 10시간으로 가장 짧았으며, 함수율도 시간 별로 일정하게 감소하여 가장 효율적인 건조방법으로 나타났다. drying rate 또한 순환형 감압 건조의 변화 폭이 가장 짧고 안정적으로 감소하는 것으로 나타났다. 이에 따른 건조 수율 또한 가장 높게 나타났다. pH, 적정산도 및 염도 측정은 순환형 감압 건조 김치가 일반 건조 전 김치와 비교하여 비슷한 수치를 보여 특성 변화 없이 안정적으로 건조가 되었다. 건조 김치의 색도는 순환형 감압 건조 김치의 적색도가 일반 건조 전김치와 가장 비슷한 수치를 나타내어 pH, 적정산도 및 염도의 결과와 함께 가장 안정적으로 건조된 것을 알 수 있었다. 또한 향기 성분의 분석에서도 건조 전 김치의 황화물과 ethanol 및 acetic acid 등의 성분함량과 비슷한 수치를 나타내어 순환형 감압 건조 과정에 있어 김치의 풍미를 유지하였다. 관능 평가의 경우 색과 Texture에서 감압 건조 김치가 가장 우수한 것으로 나타났다. 따라서 순환형 감압 건조 방법을 이용하여 김치 등의 식품을 건조하는 것이 가장 안정적이고 우수한 건조 방법으로 나타났다. 이러한 건조 김치의 품질 평가를 통하여 순환형 감압 건조 김치가 가장 안정적이고 효율적인 건조 방법이라는 것을 알 수 있었으며 이러한 건조 방법을 이용하여 김치를 상품화 한다면 기능성 식품으로 다양한 식품으로 활용하는데 가능성이 큰 것으로 기대된다. This study aimed to economically produce high quality of dried kimchi through a cyclic low pressure drying device in order to overcome the disadvantages of the conventional drying process. The moisture content of dried kimchi obtained from the cyclic low pressure drying process was found to be uniformly reduced according to drying time. Also, cyclic low pressure drying showed the shortest change and a stable reduction in drying rate. Further, cyclic low pressure drying did not markedly change pH, titratable acidity, or salt content. In addition cyclic low pressure drying compared with fresh kimchi showed the similar color values and maintained the flavor of the kimchi. In conclusion, cyclic low pressure drying resulted in superior rehydration and sensory scores.