금은화에서 분리한 Kaempferol-3-O-glucoside의 글루타메이트로 유도시킨 산화적 스트레스에 대한 HT22 신경세포 보호활성

마충제 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.4

Background: In this study, we aim to test the neuroprotective activity of kaempferol-3-O-glucoside in HT22 cells originating from mice hippocam against glutamate induced oxidative stress and to elucidate the mechanisms of action of this neuroprotective activity. Methods and Results: Pretreatment of HT22 cells with kaempferol-3-O-glucoside increased cell viability after exposure to glutamate induced oxidative stress. Kaempferol-3-O-glucoside decreased reactive oxygen species and intracellular Ca2+ concentration, which increased with glutamate induced oxidative stress. Moreover, kaempferol-3-O-glucoside maintained the mitochondrial membrane potential at control levels, increased glutathione reductase and glutathione peroxidase levels, and synthesized high levels of the endogenous antioxidant glutathione. Conclusions: Overall, these results suggest that kaempferol-3-O-glucoside isolated from Lonicera japonica exerted a potent neuroprotective activity in glutamate injured HT22 cells through the antioxidative pathway and can be potentially used for the treatment or prevention of Alzheimer's disease.

금은화에서 분리한 luteolin의 신경세포보호 활성

마충제,김은서 한국생약학회 2022 생약학회지 Vol.53 No.1

In the previous study, we reported that luteolin isolated from Lonicera japonica methanolic extract had potent neuroprotective activities in neuronal cell death injured by excessive glutamate. In this study, we tried to confirm the neuroprotective activities of luteolin in glutamate injured HT22 cells and establish mechanisms of neuroprotective action of luteolin. We used HT22 cell death injured by glutamate as a bioassay system. Luteolin decreased reactive oxygen species increased by excessive glutamate treatment in HT22 cells. Also, Ca2+ concentration was decreased by luteolin treatment. Luteolin made mitochondrial membrane potential maintain to normal condition. It also increased not only glutathione reductase but also peroxidase to the control level. And it increased amount of glutathione, an endogenous antioxidant. These results suggested that luteolin isolated from L. japonica showed potent neuroprotective activity through the anti-oxidative pathway.

금전초에서 분리한 cynaroside의 신경세포보호 활성

마충제,류가희 한국생약학회 2023 생약학회지 Vol.54 No.1

In the previous study, we reported that cynaroside isolated from Lysimachia christinae methanolic extract had potent neuroprotective activities in neuronal cell death injured by excessive glutamate. In this study, we tried to confirm the neuroprotective activities of cynaroside in glutamate injured HT22 cells and establish mechanisms of neuroprotective action of cynaroside. We employed HT22 cells damaged by glutamate-induced cell death as a bioassay system. Cynaroside decreased reactive oxygen species increased by excessive glutamate treatment in HT22 cells. Also, Ca2+ concentration was decreased by cynaroside treatment. Cynaroside restored mitochondrial membrane potential to normal condition. It also increased not only glutathione reductase but also peroxidase to the control level. And it increased amount of glutathione, an endogenous antioxidant. These results suggested that cynaroside isolated from L. christinae showed potent neuroprotective activity through the anti-oxidative pathway.

증숙에 따른 소시호탕의 활성 변화

마충제,원진배,양혜진,이보형,윤보라,마진열 한국생약학회 2011 생약학회지 Vol.42 No.3

Sosihotang is a herbal medicine traditionally used to treat chronic liver disease. This study was performed to investigate the effect of steaming Sosihotang at a 121℃ on its major compounds and neuroprotective activity. We evaluated quantitative changes of 6 major compounds in Soshihotang, saikosaponin A, homogentisic acid, baicalin, ginsenoside Rg_3,glycyrrhizin and 6-gingerol using HPLC-DAD. After steaming produce, saikosaponin A, homogentisic acid, baicalin, glycyrrhizin and 6-gingerol in Sosihotang were decreased and ginsenoside Rg_3 was increased. The neuroprotective activity of steamed Sosihotang was determined by MTT assay, comparing to Sosihotang. The steamed sosihotang showed a higher neuroprotective activity than Sosihotang. In conclusion, steam produce could improve neuroprotective activity of Soshihotang.

오약순기산 발효물의 성분 분석 및 뇌세포 보호 활성

마충제,원진배,마진열,양혜진 한국생약학회 2011 생약학회지 Vol.42 No.1

Korean traditional prescription, Oyaksungisan was usually used to treat rheumatoid arthritis, paralysis. In this study,we compared the contents of five major components, ephedrine HCl, ferulic acid, hesperidin, 6-gingerol, glycyrrhizin and various unknown compounds in Ohyaksungisan (OY) with fermented Ohyaksungisan (FOY) by established HPLC-DAD method. The neuroprotective activity against glutamate-induced cytotoxicity in HT22 cell of OY and FOY was investigated by MTT assay. And anti-oxidative activity of OY and FOY was measured by DPPH radical, hydrogen peroxide and hydroxyl radical scavenging activity. After fermentation, contents of five major components and some unknown compounds (1), (2), (3) and (4)were increased in FOY. Also, FOY showed more potent neuroprotective activity. Furthermore, anti-oxidative activity of FOY was increased. In conclusion, the fermentation of OY could change contents various compounds and improved its neuroprotective activity.

여드름 원인균 Propionibacterium acnes에 대한 생약 추출물의 항균활성 측정

마충제,원진배,안주희 한국생약학회 2011 생약학회지 Vol.42 No.1

Acne, one of the most common skin disease, is a chronic inflammatory disease and is caused by uncertain and multiple factors. Propionibacterium acnes belongs to the human cutaneous normal flora and is a major etiologic agent of acne vulgaris. In this study, we evaluated the antibacterial effect of 19 medicinal plants. Antibacterial activity of extracts prepared from the 19 medicinal plants was investigated against bacteria related to acnes, Propionibacterium acnes. Among them, some medicinal plants inhibited the growth of the P. acnes. Minimum inhibitory concentration (MIC) of Polygonum aviculare (Herb) was 0.2 mg/ml against P. acnes. MIC of Dianthus chinensis (Aerial Part), Forsythia viridissima (Fruit), Lygodium japonicum (Spore) and Sophora flavescens (Root) were 0.4 mg/ml. Based on these results, Polygonum aviculare (Herb), Dianthus chinensis (Aerial Part), Forsythia viridissima (Fruit), Lygodium japonicum (Spore) and Sophora flavescens (Root) may be considered as a candidate for a good medicine for acne.

EBM 기반 구축을 위한 팔물탕 문헌 연구 분석

마충제,이남헌,마진열,하혜경,유영법,신현규,Ma, Choong-Je,Lee, Nam-Hun,Ma, Jin-Yeul,Ha, Hye-Kyung,Yu, Young-Beob,Shin, Hyun-Kyoo 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2

Objective : The purpose of this report was to provide the information activity and safety of Palmul-tang by analyzing domestic/international papers and theses about Palmul-tang, Methods: Domestic/international papers and theses related to Palmul-tang were reviewed and analyzed, These papers were then classified by year, experimental method and subject, Results: The following results were obtained in this study. 1. The study of Palmul-tang started from 1985 and was continuously increased. The study was mainly forcused on experimental model rather than clinical study. 2. As these studies were classified by subject, papers related to immune intensification were most abundant by 20 papers, Besides there were several papers related to cardiovascular activity, reproductive activity, anti-apoptotic activity and cerebral hemodynamics. 3. Among the papers related to immune intensification. the studies on recovery of fatigue were mostabundant by 10 papers and the studies of on immune cell and cytokine express were six. In addition to. several studies were associated with anti-cancer activity and anti-allergic activity. Recovery of fatigue was determined by measurement of fatigue markers in a living body such as lactate. CPK, pyruvate and triglyceride and assessment of exercise capability of animals such as swimming test. slopped plate test. Rota-rod test, and activity cage test after Palmul-tang treatment. 4. According to experimental data. it is supported that Palmul-tang has been used as Qi and Blood intensifier with immune intensification and recovery of fatigue. 5. The paper related to safety of Palmul-tang was only one paper which is studied on acute toxicity of Palmul-tang with experiment with ICR mouse. There was no study on evaluating safety by observing liver and kidney functions after Palmul-tang treatment Conclusion: Palmul-tang is being used in various ways associating with immune intensification. cardiovascular activity and reproductive activity. However. studies on efficacy and mechanism of Palmul-tang should be conducted at the molecular biology level and studies on safety of Palmul-tang need to be completed at the clinical level.

금전초에서 분리한 androst-16-ene-3,6-diol 의 신경세포보호 활성

마충제 한국생약학회 2024 생약학회지 Vol.55 No.1

In our prior research, we documented the robust neuroprotective activity of androst-16-ene-3,6-diol, which was extracted from the methanolic extract of Lysimachia christinae. This compound exhibited significant neuroprotection against glutamate-induced neurotoxicity in HT22 cells, which were subjected to injury caused by an excess of glutamate. In this study, our objective was to validate the neuroprotective effects of androst-16-ene-3,6-diol in HT22 cells subjected to glutamate-induced injury. Additionally, we aimed to elucidate the mechanisms underlying the neuroprotective activity of androst-16-ene-3,6-diol. Androst-16-ene-3,6-diol mitigated the elevated levels of reactive oxygen species induced by excessive glutamate treatment in HT22 cells. Furthermore, the treatment with androst-16-ene-3,6-diol led to a reduction in Ca2+ concentration. The compound also restored the mitochondrial membrane potential to its normal condition. Additionally, androst-16-ene-3,6-diol not only elevated the levels of glutathione reductase and peroxidase to those of the control but also increased the amount of glutathione, an endogenous antioxidant. These results suggested that androst-16-ene-3,6-diol isolated from L. christinae showed potent neuroprotective activity through the anti-oxidative pathway.

황금 배양 세포로부터 Phospholipase A2의 분리

마충제,김대경 한국생약학회 2009 생약학회지 Vol.40 No.1

It was previously reported that yeast elicitor transiently increased oleanolic acid and ursolic acid in Scutellaria baicalensis suspension cultures and also doubled phospholipase A2 (PLA2) activity. Thus, PLA2 was purified from the soluble fractions of S. baicalensis suspension cultures and the characters of the purified PLA2 were identified. The PLA2 was purified about 160 times compared with the starting soluble-protein extract from S. baicalensis suspension culture cells. The purified protein showed a molecular mass of about 43 kDa by SDS-PAGE. The purified plant PLA2 had a neutral pH optimum (pH 7.0) and required Ca 2+ for activity. The PLA2 activity was inhibited by mammalian PLA2 inhibitors such as 5,8,11,14-eicosatetraynoic acid (ETYA) and arachidonyl trifluoromethyl ketone (AACOCF3).

글루타메이트에 의해 산화적 스트레스를 받은 HT22 세포에서 핑거루트의 신경세포 보호활성

김은서,마충제,Kim, Eun Seo,Ma, Choong Je 한국생약학회 2022 생약학회지 Vol.53 No.2

Excessive glutamate causes oxidative stress in neuronal cells, which can cause degenerative neurological disorders. We tried to find medicinal plant showed neuroprotective activity by using glutamate-injured HT22 cell as a model system. In this study, we found that Boesenbergia rotunda methanol extract showed neuroprotective activity against glutamate induced neurotoxicity in mouse hippocampal HT22 cells. B. rotunda methanol extract suppressed the formation of reactive oxygen species and decreased intracellular Ca²⁺concentration. Also, B. rotunda made mitochondrial membrane potential maintain to normal levels. In addition, B. rotunda increased total glutathione amount and activated antioxidative enzyme such as glutathione reductase and glutathione peroxidase compared to glutamate-treated groups. These results suggested that B. rotunda decreased neuronal cell death damaged by high concentrations of glutamate treatment, via antioxidative mechanism and might be one of candidate of development of new drug to treat neurodegenerative disease such as Alzheimer's disease.