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Preparation of La-Ni Alloy Membrane for Separation of H<sub>2</sub>-N<sub>2</sub> Gas Mixture
백옥진,강태범,이현경 한국공업화학회 2001 한국공업화학회 연구논문 초록집 Vol.2001 No.-
La-Ni계 합금을 제조하고 flash-evaporation 방법에 의해서 porous stainless steel판에 La-Ni 합금을 엷게 코팅하여 La-Ni alloy membrane을 제조하였다. 제조된 막을 이용해서 기체 분리압력을 2atm~6atm으로 변화시키고, 분리온도를 30℃~100℃ 로 하여 H<sub>2</sub>-N<sub>2</sub> 혼합기체를 분리하고 gas chromatography에 의해서 분리 후의 기체의 조성을 분석하였다.
곡류 중 T-2 및 HT-2 독소 동시 정량분석의 유효성 검증 및 실태조사
백옥진,강태범 한국식품저장유통학회 2015 한국식품저장유통학회지 Vol.22 No.4
The aim of this study was to develop an analytical method for determination of T-2 toxin and HT-2 toxin level in cereals and to survey their levels using LC-MS/MS. The T-2 and HT-2 toxins were simultaneously analyzed by electrospray ionization with a positive ion mode and multiple reaction monitoring (MRM) after filteration and immuno-affinity column clean-up. A matrix-matched standard calibration used for quantification and recoveries of T-2 and HT-3 toxins were in the range of 100.6±7.2% and 96.8±9.4%, respectively. Limits of detection and quantification of T-2 and HT-2 toxins were estimated to be 0.5 and 1.5 μg/kg, respectively. Each repeatability (RSRr) of T-2 and HT-2 toxins was determined to be 0.9~6.0%, and 4.9~6.1%, respectively. Total 115 samples cereals were collected from 9 types of cereals for analysis. The positive percentages of T-2 and HT-2 toxins obtained from collected samples were found to be 72% and 80%, respectively. The contamination level of T-2 toxin and HT-2 toxin in cereals were 37.1 μg/kg, and 5.4 μg/kg, respectively. Therefore, this study suggests that the developed method could be an useful analytical method to determine the T-2 and HT-2 toxin level in cereals and the present data could be used as a reference to estimate the risk assessment.
LC-MS/MS를 이용한 돼지고기 중 총아플라톡신 및 오크라톡신 A 동시분석법 확립
백옥진,박송이,박기훈,김신희,서세정,윤혜정 한국식품위생안전성학회 2016 한국식품위생안전성학회지 Vol.31 No.3
Aflatoxins and ochratoxin A (AFTs and OTA) are secondary fungal metabolites produced by several moulds, mainly by Aspergillus flavus by Aspergillus ochraceus and Penicillium verrucosum, and these toxins can be transferred to animals and humans through the ingestion of contaminated feed and food. This study was to develop the analytical method for determination the levels of AFTs (B1, B2, G1 and G2) and OTA in pork. The AFTs and OTA were analyzed simultaneously by electrospray ionization in positive ion mode and mass reaction monitoring (MRM) after solid phase extract (SPE) columns clean-up. Performance characteristics, such as accuracy, precision, linear range, limit of detection (LOD) and quantification (LOQ), were also determined. Matrix-matched standard calibration was used for quantification, obtaining the recoveries in the range of 67.3~108.2% with the relative standard deviations of < 20%. Limits of detection and quantification were also estimated, obtaining the limits of quantification ranged in 0.7~1.3 μg/kg. The results of the inter-day study, which was performed with pork samples for 3 days, showed an accuracy of 92.0~109.9%. The precisions (expressed as relative standard deviation values) for the inter day variation were 2.6~17.8%. The method developed in this study was able to carry out the analysis with the satisfactory intensity and accuracy.
백옥진 ( Ock Jin Paek ),김형수 ( Hyung Soo Kim ),서정혁 ( Jung Hyuck Suh ),강영운 ( Young Woon Kang ),이준구 ( Joon Goo Lee ),봉영훈 ( Young Hoon Bong ),최훈 ( Hoon Choi ),곽지연 ( Ji Yeon Kwak ),이승일 ( Seung Il Lee ),박기훈 ( 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-
Alternaria toxins haver been found to be natural food contaminants in grains, sunflowers seeds, and some visibly decayed fruits in many countries. Their natural occurrence in cereals has been reported in different countries. Several studies have reported the relevance of the this genus in crop. However, there are no studies on the method validation of alternaria toxin in cereals in our contry. A simple and sensitive analytical method based on HPLC with PDA and mobile phases using 0.1% phosphoric acid and acetonitrile was developed for simultaneous determination of alternaria toxins. A method validation for the determination of alternaria toxins in cereals was validated. In short, the method is as follows : A test portion of a sample is extracted with a mixture of methanol/water(90/10, v/v). This raw extract is then diluted, filtered, and applied to an SPE column. After washing and elution with acetonitrile, the elute is evaporated to dryness. After toxins in the dry residue in mobile phase are injected into a high performance liquid chromatography, and detected and quantified by PDA. For alternaria toxins in the cereals, Recovery test, calibration curves (Linearity), LOD and LOQ were successfully confirmed and Reproducibility relative standard deviations(RSDR) and Repeatability relative standard deviations(RSDr) for cereals samples were below 15% for the spiked cereals at 100 ug/kg. Since all these parameters lie well within the acceptable range set forth in EU mycotoxin method validation legislation. This method is The proposal method is sensitive, repeatable and rapid enough to apply to officially routine inspection of agricultural products including cereals.
곡류 중 트리코테센 A형 곰팡이독소의 결정론적 위해평가
백옥진 ( Ockjin Paek ),윤석일 ( Seok Il Yun ) 한국산업식품공학회 2015 산업 식품공학 Vol.19 No.4
본 연구는 백미 등 9품목에서 트리코테센류 A형 곰팡이 독소인 T-2와 HT-2 독소의 함량을 구하고 전체인구집단의 곡류 섭취로 인한 트리코테센류 A형 곰팡이독소인 T-2와 HT-2 독소의 노출 수준에 따른 위해성을 평가하고자 하였다. 우리나라 전체 인구집단의 트리코테센류 A형 T-2와 HT-2 독소의 노출수준을 산출하기 위해 결정론적 위해평가를 수행하였다. 산출된 노출량은 JECFA에서 제시한 TDI (100 ng/kg b.w./day) 대비 위해도(%)를 구하여 노출수준에 대한 위해정도를 파악하였다. 9품목 곡류 115건에 대한 트리코테센류 A형 곰팡이독소인 T-2와 HT-2 독소의 함량은 LC-MS/MS를 이용하여 구한 결과 트리코테센류 A형 곰팡 이독소인 T-2와 HT-2 독소의 합으로 평균 7.80 μg/kg (0.00-7.80) 수준으로 검출되었다. 곡류섭취로 인한 전연령의 평균섭취량을 고려하였을 때 트리코테센류 A형 곰팡이 독소인 T-2와 HT-2 독소 섭취량은 0.57 ng/kg b.w./day (LB), 1.39 ng/kg b.w./day (MB), 2.2 ng/kg b.w./day (UB)으로 TDI 대비 0.57% (LB), 1.39% (MB), 2.2% (UB) 수준 이었다. 극단(P95) 섭취량을 고려하였을 때 트리코테센류 A형 곰팡이독소인 T-2와 HT-2 독소 섭취량은 11.06 ng/kg b.w./day (LB), 13.95 ng/kg b.w./day (MB), 16.87 ng/kg b.w./ day (UB)으로 TDI 대비 11.06% (LB), 13.95% (MB), 16.87% (UB) 수준이었다. 따라서, 우리나라 국민들의 곡류섭취로 인한 트리코테센류 A형 곰팡이독소인 T-2와 HT-2 독소 노출수준은 위해 우려가 낮은 수준으로 평가되었다. This research was conducted to estimate the dietary exposure of trichothescene type A mycotoxins (T-2 and HT-2 toxin) and to assess the related risk for the Korean populations from the intake of cereals. The deterministic approach was used to estimate the dietary exposure. The amounts of trichothescene type A mycotoxins in 115 samples of cereals were determined by LC-MS/MS. The levels of trichothescene type A mycotoxins (T-2 and HT-2 toxin) in cereals ranged from: N.D.-37.10 ug/kg for T-2 toxin and N.D.-5.43 μg/kg for HT-2 toxin. The mean and 95th percentile for exposure to the dietary sum of trichothescene type A mycotoxins were 0.57 ng/kg b.w/day (LB), 1.39 ng/kg b.w/day (MB), 2.20 ng/kg b.w/day (UB), and 11.06 ng/kg b.w/day (LB), 13.95 ng/kg b.w/day (MB), 16.87 ng/kg b.w/day (UB) corresponding to 0.57 to 2.20% of the Total Dietary Intake (TDI) for the mean exposure and 11.06 to 16.87% of TDI for the 95th percentile exposure. Therefore, the overall exposure level of trichothescene type A mycotoxins for Koreans from cereal intake is unlikely to cause concerns to human health.
백옥진(Ockjin Paek),서정혁(Junghyuk Suh),박희라(Heera Park),오금순(Keumsoon Oh),홍세령(Selyung Hong),이현경(Hyunkyung Lee),김미혜(Meehye Kim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.3
Polychlorinated biphenyls (PCBs) have been studied during the past few decades because of their potential impacts on the environment and human health. PCBs are toxic environmental pollutants and persistent organic pollutants (POPs). This study was carried out to assess the dietary exposure and risk to PCBs for the general Korean population through food intake. Various samples (n=389) covering 28 kinds of food were collected from 5 cities in Korea. The PCB content was not detected-182.4 ㎍/㎏ (mean of 5.4 ㎍/㎏) in the food. The mean dietary exposure of PCBs for the general population was 9.54 ng/㎏ bw/day with an intake of 0.19% of tolerable daily intake (TDI) (5 ㎍/㎏ bw/day). Therefore, the level of overall dietary exposure to PCBs for the Korean population through food intake is below the recommended TDI levels.