LED광원에 따른 감초 지상부의 생리활성 및 성분 평가

방극수,장영남,진종식,박상아,임재수,박정섭,김종성,이정호 한국자원식물학회 2015 한국자원식물학회지 Vol.28 No.1

Glycyrrhiza uralensis Fischer is one of the most commonly used herbs. Recently, the stem and leave of the planthave been interested in physiological activities because the aerial parts have been thrown away. Finding out cultivationmethod of Glycyrrhiza uralensis Fischer to improve chemical ingredients and biological activities has been tried these days. In this study, different wavelengths of light emitting diode (LED) were used for a cultivation of Glycyrrhiza uralensisFischer. Antioxidant activities and inhibitory effect on mutagenecity of samples were evaluated. The stem and leave cultivatedunder blue light (BL-0) showed the strongest antioxidant activities of 3.02 ± 0.13 ㎍/㎖ (EC50) and 2.18 ± 0.18 ㎍/㎖ (EC50)in DPPH and ABTS radical scavenging test, respectively. Total phenolic content of BL-0 was 2.93 ± 0.11 g/100 g, thehighest value between cultivation conditions. However, antioxidant activities of the stem and leave cultivated under redlight were the weakest between samples. All of the stem and leave used in this study showed inhibitory effect onmutagenecity of 1-nitropyrene. BL-0 showed stronger inhibitory effects on mutagenicity of Trp-P-1, Trp-P-2, and AFB1than samples cultivated under other conditions. Only on mutagenecity of 2-aminoanthracene, the stem and leave cultivatedat 1 m apart from red light (RL-1) showed the strongest inhibitory effect. These results indicate that blue LED might be themost effective condition for improvement of physiological activities for the aerial parts of Glycyrrhiza uralensis Fischer incultivation. The components were identified with GC/MS. Cytidine was detected only in RL-1 at 25 min of retention timeand 2-bromotrimethylene glycol was detected only in BL-0 at 37 min. LED 광원을 달리하여 재배한 감초지상부의 항산화, 항돌연변이원성을 평가하였다. BL-0에서 DPPH 라디칼 소거활성에서 EC50이 3.02 ± 0.13 ㎍/㎖와 ABTS 라디칼 소거활성은 EC50이 2.18 ± 0.18 ㎍/㎖와 환원력이 가장 높게 나타났다. 폴리페놀함량은 2.93 ± 0.11 g/100 g으로 가장 높게 나타났으며, RL-0에서는 항산화 활성이 가장 낮게 나타났다. 항돌연변이원성 평가에서 직접변이원인 1-NP에 대해 모든 실험군에서 돌연변이억제효과를 나타냈으며, 간접변이원인인 Trp-P-1, Trp-P-2,AFB1에 대해서는 BL-0에서 가장 높은 돌연변이 억제효과를 나타냈고, 2-AA에서는 RL-1에서 돌연변이 억제가 높게 나타났다. 청색광 LED (650 ㎚)와 적색광 LED (450 ㎚)를 이용하여 재배한 감초지상부의 성분변화를 관찰하기 위해 GC/MS를 이용해 분석한 결과 25분에서 RL-1에서만 cytidine이 검출되었고,37분에서 BL-0에서만 2-bromotrimethylene glycol이 특정적으로 검출되었다. 이는 LED광원 조건에 따라 감초 경엽의 성분이 차이가 있음을 보여준다. 이 성분들이 생리활성에 어떤 영향을 주는지는 더 검토해야할 부분이다.

植物生長調節劑가 고구마의 器內 不定胚發生에 미치는 效果

李重浩,李萬相,房極秀 圓光大學校大學院 1989 論文集 Vol.3 No.-

고구마 White star 品種의 生長點 培養에서 培養組織의 部位 및 植物生長調節劑의 種類 및 濃度가 器內 不定胚發生과 植物體 再分化에 미치는 效果를 調査하였던바 그 結果를 要約하면 다음과 같다. 純粹한 meristem dome만을 培養했을때 embryogenic callus의 유기효과는 2,4-D 0.1mg/ℓ를 添加한 MS基本培地에서 96% 誘起되었으며, embryogenic callus로 부터 植物體 再分化는 hormone free 培地에서 培養했을 때 가장 效果的이었으며, cytokinin중 kinetin이 BAP, zeatin, BA에 비하여 胚의 發育과 再分化에 促進的이었고 zeatin 添加區에서 培養한 somatic embryo는 97% 以上이 heart stage의 子葉部에서 分化되었다. This studies were carried out to examine the effect of explant sources and various concentration of growth regulators on the somatic embryogenesis and plantlets regeneration. The results obtained were summarized as follows; Embryogenic calli were obtained with the rate as high as 96 percent from shoot tip meristem devoid of leaf primordia when cultured on Murashige and Skong's medium supplemented with 0.1mg/ℓ 2,4-D. The effect of plantlets regeneration were promoted when the embryogenic call were transferred to hormone-free medium. The effect of kinetin on the development of somatic embryo was better than the BAP, BA, zeatin. Frequency of uniform somatic embryos tended to increase on MS medium supplemented with 0.5mg/ℓ abscisic acid. When the embryoginic callus was cultured on the secondary medium supplemented with zeatin, many of the embryos gave rise to the secondary embryos, as many as 97 percent of than arising from the cotyledonary primordia of the heart stage embryos.

小豆의 植物體再分化에 關한 硏究

李重浩,房極秀 圓光大學校 1988 論文集 Vol.22 No.2

小豆 幼苗의 莖頂培養에서 植物生長調節劑가 callus形成과 器官分化에 미치는 影響을 調査하고자 auxin (NAA)과 cytokinin 類 (BAP, zeatin)등을 Murashige와 Skoog의 基本培地에 添加하여 實驗을 遂行하였던 바, 다음과 같은 結果를 얻었다. 小豆의 莖頂培養에서 callus誘起가 가장 좋은 培地는 NAA 0.2㎎/ℓ와 BAP 1㎎/ℓ를 混用한 것이었으며, 植物體再分化가 가장 잘되는 培地는 NAA 0.5㎎/ℓ와 BAP 0.5㎎/ℓ를 添加한 것이었다. 胚狀體形成은 zeatin을 添加한 培地에서만 볼 수 있었고, NAA 1㎎/ℓ와 zeatin 0.1∼0.5㎎/ℓ를 添加한 培地에서 가장 잘 形成되었지만 zeatin의 添加區에서 얻어진 胚狀體는 植物體形成이 늦은 傾向이었다. The studies were carried out to examine the effects of NAA, BAP, and zeatin supplemented to Murashige and Skoog's medium on the callus formation, organogenesis and embryogenesis in the shoot apex culture of adzuki bean (Vigna angularis W. F. WIGHT). The best medium for callus formation was the modified Murashige and Skoog's medium containing 0.2㎎/ℓNAA and 1㎎/ℓBAP, and the best medium for plantlet regeneration was the modified Murashige and Skoog's medium containing 0.5㎎/ℓNAA and 0.5㎎/ℓBAP. The effect of zeatin on the embryoid formation from the adzuki bean calli was recognized. These embryoids were formed on the modified Murashige and Skoog's medium containing 1㎎/ℓNAA and 0.1-0.5㎎/ℓzeatin, but the plant formation from embryoids was late.

생장조절제가 백합의 기내증식에 미치는 영향

방극수(K . S . Bang) 원광대학교 생명자원과학연구소 1991 농대논문집 Vol.14 No.-

고구마의 기내 부정배발생에 관한 기초연구

방극수(K . S . Bang),이만상(M . S . Lee),이중호(J . H . Lee) 원광대학교 생명자원과학연구소 1988 농대논문집 Vol.11 No.-

Extraction, characterization, and catalytic potential of amorphous silica from corn cobs by sol-gel method

PalanivelVelmurugan,방극수,심재홍,이귀재,조민,임성식,서상기,조광민,오병택 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.29 No.-

A simple method based on alkaline extraction followed by acid precipitation can be used to form silicaaquagel from corn cob ash (CCA). SEM-EDS analysis confirmed the high silicon and oxygen content of theextracted silica. XRD diffraction indicated that the obtained product was amorphous silica, while FTIRdata demonstrated the presence of siloxane and silanol groups. TEM images revealed the silica to beamorphous, and have an agglomeration form with an average diameter of 50 nm. CCA-extracted silica(0.1 g/20 ml) was observed to have good catalytic potential with 100% removal of 12 10 4 Mmethylene blue.

방풍통성산의 비만에 대한 약리학적 고찰 - 일본의 연구를 중심으로 -

박상아,방극수,장영남,이정호,이종현,진종식,Park, Sang-Ah,Bang, Keuk-Soo,Chang, Young-Nam,Lee, Jeong-Ho,Lee, Jong-Hyun,Jin, Jong-Sik 대한한의학방제학회 2014 大韓韓醫學方劑學會誌 Vol.22 No.2

Objectives : Excessive nutrition intake and lack of physical activity induce obesity through accumulation of fat in human body. The obesity have been categorized as a disease and known to cause several chronic diseases and cancers. In Japan, Bangpungtongsung-san has been applied as one of the therapies of the obesity and it's pharmacological uses have been studied for a long time. The purpose of this study is reviewing researches of Bangpungtongsung-san related to the obesity. Methods : By searching with the keyword of "Bangpungtongsung-san" or related words such as "Bofutsushosan" and 'TJ-62" from PubMed and off-line libraries, many reports were gathered and reviewed from the perspective of the obesity. Results and Conclusions : It has become evident by reviewing the reports that Bangpungtongsung-san is useful as a therapy of the obesity though some case reports showed side effects. Also, the mechanisms of Bangpungtongsung-san on obesity were reviewed and suggested.

지리오가피(Acanthopanax chiisanensis) 체세포배의 건조처리를 통한 식물체 증식

이강섭,방극수,최용의,안병룡 한국식물생명공학회 2003 JOURNAL OF PLANT BIOTECHNOLOGY Vol.30 No.4

지리오가피 (Acanthopanax chiisanensis) 체세포배의 건조처리를 통한 식물체 증식

이강섭,방극수,최용의,안병룡,Lee, Kang-Seop,Bang, Keuk-Soo,Choi, Yong-Eui,Ahn, Byung-Yong 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.4

지리오가피의 체세포배로부터 식물체재생을 위한 효율적인 방법을 개발하였다. 체세포배의 형태형성에 미치는 식물생장 조절물질의 영향을 조사하고자, 접합자 배 유래의 배발생 세포를 MS배지에 현탁배양하고, 이로부터 얻어진 자엽시기의 배를 배양접시에서 건조처리 (0∼72 h)한 후, NAA, BA, GA$_3$(0∼0.5mg/L)가 첨가된 MS배지에 배양하였다. 그 결과, 체세포배를 72시간 동안 배양접시에서 건조처리 후에 0.5mg/L NAA, 0.5mg/L BA 또는 0.5mg/L NAA, 0.5mg/L BA 0.5mg/L GA$_3$가 첨가된 MS배지에 6주간 배양하였을 때, multiple shoot가 100%로서 가장 높게 유도됨을 관찰 할 수 있었다. 가장 높은 식물체의 재생율 (29.1%)은 체세포배를 72시간 건조처리 후에,0.1mg/L NAA, 0.1mg/L BA그리고 0.1mg/L GA$_3$가 첨가된 MS배지에 6주간 이식한 후, 이를 다시 1.0mg/L GA$_3$가 첨가된 1/3MS배지에 6주간 배양하였을 때 이루어졌다. 유식물체를 vermiculite와 sand가 1:1로 첨가된 plastic pot 이식하였을 때 생존율은 약 70%로서 나타났다 이상의 결과는 지리오가피의 계량생산에 활용될 수 있을 것으로 기대된다. An efficient method of plant regeneration from Acanthopanax chiisanensis somatic embryos was developed. Cotyledonary somatic embryos were obtained in liquid Murashige and Skoog (MS) medium from embryogenic cell suspension cultures. They were desiccated for 0 to 72 hr and then cultured on MS medium containing NAA, BA, GA$_3$, (0-0.5mg/L). The highest multiple shoots formation (100%) was obtained from 72 hr desiccated somatic embryos on ifs medium with 0.5mg/L NAA+0.5mg/L BA or 0.5 mg/L NAA+0.5mg/L BA+0.5mg/L GA$_3$ after 6 weeks culture. Plant conversion from multiple shoots was not high. The highest plant conversion from multiple shoots was obtained on 1/3MS medium with 1.0mg/L GA$_3$. Converted plantlets were transferred to ex vitro condition and the highest survival rate (70%) of the plantlets was obtained on plastic pots containing vermiculite and sand. These results indicate that micropropagation procedure can be applied for an efficient mass propagation of Acanthopanax chiisanensis.

멸종 위기에 처한 Bulbophyllum auricomum Lindl. orchid의 시험관 내 종자 발아를 통한 구근 절편체의 식물 재생 및 캘러스 유도에 대한 천연 추출물 및 식물 성장 조절제(PGR)의 효과

땡기아웅,방극수,윤서아,고바울,배종향 (사) 한국생물환경조절학회 2022 생물환경조절학회지 Vol.31 No.2

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L-1 of BAP and 2.0 mg·L-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L-1 of banana and 20 g·L-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L-1 of coconut water) and plant growth regulators (2.0 mg·L-1 of BAP and 1.0 mg·L-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively. Bulbophyllum auricomum Lindl.은 희귀 난초이며 매력적인 향기가 지닌 꽃이 피어난다. 본 연구는 미세 증식을 위한 조직 배양 방법을 조사하였다. 인공 자가 수분으로부터 파생된 캡슐은 MS 기본 배지에서 최상의 종자 발아를 위해 얻어진다. 식물 성장 조절제(1.0 mg/L의 BAP 및 2.0 mg/L의 NAA)는 원괴체 유래 유사구근 절편체의 캘러스 유도에 영향을 받았다. 캘러스 계대배양을 위해 10가지 처리 배지에서 다른 천연물 추출물을 테스트하였다. 이 중 150ml/L 코코넛 워터를 첨가한 MS 배지는 일반적으로 1개월 및 2개월에 생중량(1.75 ± 0.08) 및 (3.01 ± 0.20) 캘러스 증식 및 PLB 유도에 효과가 있었고, 그 다음으로 바나나 30g/L와 감자 추출물 20g/L의 MS 조합이 뒤를 이었다. 식물 생장 조절제와 천연 추출물을 함께 첨가한 MS배지와 천연 추출물만 첨가한 MS 배지를 비교 연구한 결과, 천연 추출물(코코넛 워터 150ml/L)과 식물 생장 조절제(BAP 2.0 mg/L 및 NAA 1.0 mg/L)를 혼합하여 MS 배지에 첨가한 결과 ​배양 1개월 및 2개월 후에 각각 가장 높은 새싹 재생수로 (3.37 ± 0.17) 및 (6.41 ± 0.68)을 얻었다.