http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
토양전염성 맥류바이러스 발생지역 및 맥류품종의 저항성 조사
이귀재,김형무,이왕휴 한국자원식물학회 2001 한국자원식물학회지 Vol.14 No.3
Soil-borne barley viruses were investigated in 42 regions during 1999-2003, in Korea. BaYMV, BaMMV and SbWMV symptoms were found in 37 regions. Crops resistance to mosaic viruses were investigated by growing them on 10 regions infected with soil-borne viruses. It was found that 10 unhulled barley, 6-beer bar]ey, 16 rye and 2 wheat species showed resistance to virus. Most of 15 Japanese cultivars showed infection symptoms but many of them showed relatively higher resistance in Ikasan, Youngkwang and Yesan areas region. Mixed virus infection was investigated and it was found that inter-regional species except in 10 areas, were mix infected with BaYMV and BaMMV. But in Youngkwang area all of crop species except Secheon-6 were infected only with BaYMV. Japanese cultivars were mixed infected with BaMMV and BaYMV except at Yesan that were infected only with BaYMV. 1999년부터 2001년까지 전국42개 지역에서 토양전염성 맥류바이러스를 조사한 결과 강원도 영월, 전라북도 무주, 충청남도 공주, 보령, 서천 포장을 제외한 37개 포장에서 BaYMV, BaMMV 및 SbWMV가 발생하고 있었다. 또한 토양전염성 맥류바이러스 상습 발병지 10개 장소에 겉보리 10품종, 쌀보리 16품종, 맥주보리 6품종, 밀 2품종을 파종하고 저항성 조사를 실시한 결과 겉보리는 조강보리, 쌀보리는 내한쌀보리 및 논산과1-6품종이 비교적 여러지역에서 저항성 높은 품종으로 나타났다. 그리고 일본에서 분양 받은 15품종도 대부분이병성으로 나타났으나 특이하게 익산, 영광 및 예산지역에서 저항성 품종이 많았다. 지역간 품종에 따른 바이러스 혼합 감염을 조사한 결과 조사 10개 지역 모두 BaYMV, 및 BaMMV가 혼합 감염되어 있었고, BbWMV는 예산, 진주, 나주 및 익산지역에 혼합감염되어 있었다. 그러나 영광지역에서는 사천6호 품종을 제외하곤 BaYMV만이 발생하였다. 일본 분양 품종은 예산에서만 BaYMV가 감염되었고 나머지 지역에서 모두 BaMMV 및 BaYMV에 혼합 감염되 어 있었다.
RP-PCR을 이용한 보리누른모자이크바이러스 (BaYMV)와 보리마일드모자이크바이러스(BaMMV)의 외피단백질 유전자 검정 및 해석
이귀재 한국식물병리학회 1998 Plant Pathology Journal Vol.14 No.4
Using the reverse transcription polymerase chain reaction (RT-PCR), a rapid and sensitive assay method for the detection and identification of barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) was adapted. Two units of primers from each virus were selected and used for the determination of two different viruses. PCR fragments of BaYMV (ca. 0.9kb) and BaMMV (ca. 0.8kb) were obtained from the designed method for the assay of BaYMV and BaMMV coat protein. PT-PCR fragments were cloned using vector pT7 Blue and the sequences of the selected clones were analyzed. coat protein of BaYMV and that of BaMMV consisted of 297 amino acids (891 nucleotides) and 251 amino acids (753 nucleotides), respectively. The snalysis of coat protein genes from these two viruses showed that 45.6% of nucleotides sequence ad 34.9% of amino acid in BaYMV were homologous to those in BaMMV.
감나무 가지마름病에서 分離한 Pestalotia kaki의 2 系統
李貴宰,田吉亨,金炯武,蘇仁永,崔定植 전북대학교 농업과학기술연구소 1994 農大論文集 Vol.25 No.1
A black spot and die-back symptoms were severely occurred on the branch of young persimmon tree at Chonju and Haenam between 1991 and 1992. The Pestalotia kaki isolated from symptoms were investigated in cultural character and pathogenicity. Pestalotia kaki was isolated from the black spot and die-back symptoms. Pestalotia kaki was classified into the strain I and Ⅱ by cultural character. The color of mycelium on the PDA, the strain l was white and turned yellowish in the center, and the strain Ⅱ was white. Conidia formed concentric Bones in the strain Ⅱ, and scattered in the strain Ⅰ . Pathogenicity of the strain I and Ⅱ was simillar.
유귀재,이성동,인만진,Woo Ik Hwang,Kwang Seung Lee,Eun Sil Lee,Dong Chung Kim,채희정 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.4
Two types of sequential extraction process (SEP) for the recovery of bioactive components from Korean red ginseng were examined. As a SEP (normal SEP, nSEP), Korean red ginseng was sequentially treated using hot water and n-hexane for the successive extraction of water-soluble and lipid-soluble components. Also by changing the sequential order of solvents, an alternative SEP (reverse SEP, rSEP) in which n-hexane extraction was followed by hot water extraction, was proposed. Regardless of the sequential order of solvents, the recovery yield of acidic polysaccharide (AP) and crude saponin (CS) showed no significant change. On the other hand, in the rSEP, the lipid-soluble fraction was obtained from red ginseng with an enhanced recovery yield, four times higher than that in nSEP. Additionally, from dose-response assays to assess the effects of lipid-soluble components on the proliferation of human hepatoma (HepG2) and breast (MCF-7) cancer cell lines, it was found that hexane extract of red ginseng (HER) in rSEP has higher efficacy than the hexane extract of red ginseng marc (HERM) obtained in nSEP. This strongly suggested that rSEP would be a more attractive industrial process in terms of the efficacy of lipid-soluble extract as well as the recovery yield.