Lactic Acid Bacteria Probiotics Inhibit Interaction Between Aflatoxin B1 and HT29 Colorectal Cells by Directly Binding the Toxin

박성후,정재은,Wonhee Cho,Minhee Lee 건강기능식품미래포럼 2023 건강기능식품미래포럼 학술지 Vol.3 No.1

In this study, we investigated the binding of 10 strains of lactic acid bacteria (LAB) to HT29 cells (a human colorectal adenocarcinoma cell line) and aflatoxin B1 (AFB1) and the possibility that HT29 cells and AFB1 compete for the binding site of LAB. All the 10 strains examined showed binding to HT29 cells and AFB1, among which Lactobacillus plantarum KCTC 3099 showed the highest binding to both HT29 cells and AFB1. When the binding between L. plantarum KCTC 3099 and HT29 cells was observed in the presence of various concentrations of AFB1, the binding was reduced in a concentration-dependent manner, indicating that HT29 cells and AFB1 compete for the same binding sites in L. plantarum KCTC 3099. The protoplast of L. plantarum KCTC 3099 or HCL-treated L. plantarum KCTC 3099 whose cell wall structure was changed by acid treatment did not show binding to them, suggesting that the cell wall was involved in the binding. In addition, of plantarum KCTC 3099 treated with lipase, pronase E, sodium, m-periodate or urea, m-periodate-treated L. plantarum KCTC 3099 showed the lowest biding to both HT cells and AFB1. m-Periodate is known to change the OH groups of carbohydrates. These results suggest that 1) HT29 cells and AFB1 bind to the same site in the cell wall of L. plantarum KCTC, 2) the binding site contains mainly polysaccharide components and importantly 3) biding of L. plantarum KCTC to AFB1 could reduce the amount of free AFB1 and thus, the opportunity for AFB1 to interact with the enterocytes. The binding of LAB to AFB1 is thought to be the mechanism of anti-toxic or anti-carcinogenic action of LAB probiotics against AFB1.

미숙아망막병증 검사시의 벨 현상과 결막손상의 상관관계

박성후,이지은,최희영,엄부섭.Sung Who Park. M.D.. Ji Eun Lee. M.D.. Hee Young Choi. M.D.. Boo Sup Oum. M.D. 대한안과학회 2007 대한안과학회지 Vol.48 No.12

Purpose: Bell`s phenomenon (BP) was known not to be common in premature babies. If manifested, it may be detected on screening examinations for retinopathy of prematurity (ROP), indicating that conjunctival injury may have occurred. We studied correlations with BP and conjunctival injuries. Methods: Ninety-two eyes of 46 premature babies, ranging from 36 to 38 weeks of gestational age, underwent screening examination for ROP. Rotation of the eyeball was evaluated with an inserted speculum. After observing temporal and nasal ora serrata with indentation, conjunctiva was examined for any possible damage. Results: BP was observed in 50 of 92 subjects (54%). Notable conjunctival hemorrhage was found in 7 of 50 eyes (14%) with BP and in 2 of 42 eyes (5%) without BP. Stronger BP is correlated with severe injury to the conjunctiva (p<0.001). Conclusions: Bell`s phenomenon (BP) may manifest in screening examinations for ROP. Manifestations of BP are related to conjunctival injury.

급성망막괴사에서 망막박리 발생의 위험인자

박성후,신민규,변익수,박현준,이지은,엄부섭,Sung Who Park,Min Kyu Shin,Ik Soo Byon,Huyn Jun Park,Ji Eun Lee,Boo Sup Oum 대한안과학회 2013 대한안과학회지 Vol.54 No.11

Purpose: Retinal detachment (RD) complicated in acute retinal necrosis (ARN) is difficult to be treated and a main cause of blindness. The factors associated with RD in ARN were investigated. Methods: Patients with ARN who were diagnosed and treated from Jan, 2008 to Dec, 2012 were reviewed retrospectively. The eyes were classified into the group I without RD, and the group II with RD. Early vitrectomy, history of ARN in the other eye, extent of necrosis, symptom duration and intravitreal injection of anti-viral drug were evaluated. Results: Of 22 eyes of 20 patients, 11 eyes were included in each group. Symptom duration of 8.0 days in the group I was shorter than 15.8 days in the group II (p = 0.005). There were no macular involvement at initial exam in the group I and 5 eyes (45%) in the group II (p = 0.017). Five eyes (45%) in the group I and 0 eye (0%) in the group II had history of ARN in the other eye (p = 0.017). Six eyes (55%) in the group I and 1 eye (9%) in the group II underwent early vitrectomy (p = 0.031). Age, baseline visual acuity, and intravitreal injection of antiviral agent were not related to RD (p = 0.294-0.699). Conclusions: Broader necrosis and longer symptoms duration were related to occurrence of RD. Correlation of Lower risk of RD with ARN history in the other eye would result from earlier diagnosis and treatment. Early vitrectomy seems to be effective to prevent RD in ARN. Korean Ophthalmol Soc 2013;54(11):1694-1699

소아에서 역기하렌즈 착용의 장기 임상 결과

박성후,정재호,이지은,최희영 부산대학교 병원 암연구소 2007 부산대병원학술지 Vol.- No.21

Purpose: This study was designed to investigate the effectiveness and safety of reverse geometric lens (Ortho-K LK lens) in children wearing over 12 months. Methods: Fifty eight eyes of 29 Children were enrolled and studied from March 2003 to April 2004. Uncorrected visual acuity, spherical equivalent, corneal thickness, astigmatism, anterior chamber depth and corneal endothelial cell count were measured before fitting , and 1,2,4,12,25,50 weaks after lens fitting. Results: Uncorrected visual acuity was increased to log MAR 0.03 at I week and sustained. Manifested refractive error was decreased during this period. There was not any significant change in corneal thickness, corneal endothelial cell count and anterior chamber depth. Conclusion: Reverse geometric lenses were found to be effective to correct visual acuity and safe over long period in children.

잠복외망막병증의 형태로 나타난 안매독

박성후,박종호,변익수,이지은,Sung Who Park,Jong Ho Park,Ik Soo Byon,Ji Eun Lee 대한안과학회 2014 대한안과학회지 Vol.55 No.5

Purpose: To report a case of ocular syphilis presenting as occult outer retinopathy. Case summary: A 42-year-old female presented with decreased visual acuity for one month in duration in the left eye. Her best-corrected visual acuity (BCVA) was 20/20 in the right eye and 20/250 in the left eye. There was no sign of inflammation in the anterior segment. Fundus examination showed no distinct abnormal findings including vitreous cell or haziness except mild diabetic retinopathy and subtle opacity of the macula. Spectral domain optical coherence tomography (OCT) showed a marked distortion of the inner segment-outer segment (IS-OS) junction in the photoreceptor layer without macular edema. Fluorescent angiography revealed diffuse subtle hyperfluorescence with microvasculitis in late phase. Full-field electroretinography (ERG) and multifocal ERG showed decreased amplitude; thus, acute zonal occult outer retinopathy (AZOOR) was considered as the diagnosis. Laboratory work-ups before steroid therapy revealed positive serology for active syphilis. One month after treatment with penicillin G (6 million international units per day for 14 days), best-corrected VA improved to 20/30, and restoration of the IS-OS junction was observed on OCT. Conclusions: Ocular syphilis may present as occult outer retinopathy resembling AZOOR, and serologic work-up is required to avoid misdiagnosis. In the present case, anatomical and functional recovery were obtained after antibiotic therapy. J Korean Ophthalmol Soc 2014;55(5):785-788

Impact of Age on Scleral Buckling Surgery for Rhegmatogenous Retinal Detachment

박성후,권한조,변익수,이지은,엄부섭 대한안과학회 2017 Korean Journal of Ophthalmology Vol.31 No.4

Purpose: The purpose of this study is to investigate new prognostic factors in associated with primary anatomicalfailure after scleral buckling (SB) for uncomplicated rhegmatogenous retinal detachment (RRD). Methods: The medical records of patients with uncomplicated RRD treated with SB were retrospectively reviewed. Eyes with known prognostic factors for RRD, such as fovea-on, proliferative vitreoretinopathy, pseudophakia,aphakia, multiple breaks, or media opacity, were excluded. Analysis was performed to find correlationsbetween anatomical success and various parameters, including age. Results: This study analyzed 127 eyes. Binary logistic regression analysis revealed that older age (≥35) was thesole independent prognostic factor (odds ratio, 3.5; p = 0.022). Older age was correlated with worse preoperativevisual acuity (p < 0.001), shorter symptom duration (p < 0.001), presence of a large tear (p < 0.001), subretinalfluid drainage (p < 0.001), postoperative macular complications (p = 0.048), and greater visual improvement(p = 0.003). Conclusions: Older age (≥35) was an independent prognostic factor for primary anatomical failure in SB for uncomplicatedRRD. The distinguished features of RRD between older and younger patients suggest that vitreousliquefaction and posterior vitreous detachment are important features associated with variation in surgicaloutcomes.

익상편 수술 후 발생한 메티실린 저항 황색 포도상구균

박성후,이민호,이지은,이종수,Sung Who Park,M,D,Min Ho Lee,M,D,Ji Eun Lee,M,D,Jong Soo Lee,M,D 대한안과학회 2007 대한안과학회지 Vol.48 No.1

Purpose: To report a case, which developed after pterygium excision, of methicillin-resistant Staphylococcus aureus (MRSA) scleritis, for which the patient was treated with antibiotics, debridement, and a scleral graft. Methods: During the application of topical and systemic steroid, because of necrotizing scleritis after pterygium excision, infectious signs were noted. Although the empirical antibiotic treatment with amikacin and ceftazidime was used topically and systemically, no improvement was observed. MRSA was identified by scleral culture, and topical and systemic vancomycin were applied. Results: By one week after beginning vancomycin therapy, the lesion had improved. Over the next seven days, scleral inflammation diminished, and infectious signs regressed. Surgery adding a scleral patch graft and sliding conjunctival flap was performed, and no complications or recurrences were found until eight months postoperatively. Conclusions: In cases of necrotizing scleritis treated with steroid, it should be carefully observed whether there is combined infectious scleritis. If infectious scleritis is resistant to empirical antibiotics, then it should be considered that MRSA can be etiological agent.

눈물길우회술에 사용된 후 제거된 죤스관 배양검사 결과

박성후,박준모,이지은,이종수,Sung Who Park,Jun Mo Park,Ji-Eun Lee,Jong Soo Lee 대한안과학회 2006 대한안과학회지 Vol.47 No.5

Purpose: We evaluated cultured specimens from removed Jones tubes and their antibiotic sensitivities. Methods: In 16 patients who had received lacrimal bypass surgery to resolve nasolacrimal duct obstruction, the Jones tubes were removed due to either recurred symptoms, signs of chronic dacryocystitis, the tubes being obstructed, or migration of the tubes. Jones tubes were cultured to identify bacteria and tested for their antibiotic sensitivity. Results: The average duration from intubation to removal was 11.5 months. Nine cases showed the tube obstruction and six cases showed recurrent inflammation as the respective causes of tube removal. The species of cultured bacteria were Staphylococcus aureus in 9 cases, Pseudomonas in 4 cases, Streptococcus in 2 cases and coagulase negative Staphylococcus in 2 cases. In 1 case, both Staphylococcus aureus and Pseudomonas were cultured. In antibiotics sensitivity tests, 8 of 9 cases of Staphylococcus aureus were resistant to penicillin. The symptoms and signs of nasolacrimal duct obstruction were improved after the tube removal. Conclusions: When treating an obstruction of the lacrimal apparatus due to chronic dacryocystitis using lacrimal bypass surgery with Jones tubes, Staphylococcus aureus and Pseudomonas aeruginosa should be suspected as the main cause of inflammation of the nasolacrimal system or nasal cavity.

전신홍반성루푸스에 동반된 캐슬만병 1예

박성후 ( Seong Hu Park ),이승근 ( Seong Geun Lee ),김상미 ( Sang Mi Kim ),김근태 ( Gun Tae Kim ),백승훈 ( Seung Hoon Baek ),박영은 ( Young Eun Park ) 대한류마티스학회 2013 대한류마티스학회지 Vol.20 No.1

SLE 환자에서 발생한 림프절 병증은 다양한 조직학적 소견을 보일 수 있으며 악성 림프종의 감별 진단을 위해 주의 깊은 평가를 요한다. SLE 환자에서 발생한 림프절병증에서 캐슬만병이 진단된 예가 많지 않고 그 경과에 대해 충분히 알려 지지 않았으므로 이에 저자들은 SLE 환자에서 발생한 유리체혈관형의 다중심성 캐슬만병 1예를 경험하였기에 경과 및 치료, 예후 등을 보고하는 바이다. Castleman`s disease is a rare atypical lymphoproliferative disorder. Although HHV-8 has been reported to be a cause of Castleman`s disease, the etiology and pathogenesis of the disease remains mostly unknown. We experienced a 51- year-old female patient who was concurrently diagnosed with Castleman`s disease and systemic lupus erythematosus. Castleman`s disease has been rarely reported in patients with systemic lupus erythematosus. Thus, we report the case and briefly discuss relevant articles.

의류관리기에서 스팀 분사 시간이 히트펌프 사이클에 미치는 영향에 대한 실험적 연구

박성후(Sung Hoo Park),이동찬(Dongchan Lee),김용찬(Youngchan Kim) 대한설비공학회 2022 대한설비공학회 학술발표대회논문집 Vol.2022 No.6

The cloth care system is a novel kind of home appliances that uses steam and heat pump to refresh (dust removal, deodorization, and wrinkle reduction) and sterilize cloth. Since the steam reduces wrinkle of clothes, it is necessary to inject steam to the inner case of the cloth care system. However, the steam injection affects the heat pump cycle to dry the clothes. Therefore, in this study, the effect of steam injection time on a heat pump cycle for a cloth care system was investigated. The steam injection time was controlled from 0 to 8 min while the heat pump was operated under the same conditions to check the effect of the steam injection time on the heat pump cycle. As a result, when the steam injection time increased by 1 min, the air temperature of evaporator inlet increased by 2 °C in average owing to the latent and sensible heat of the injected steam. The amount of heat transfer in the evaporator and condenser increased 27.5% and 34.7%, respectively, as the steam injection time increased from 0 to 8 min. In addition, the time required to reach 40 °C of inner case temperature decreased from 1494 to 199 s as the steam injection time increased from 0 to 8 min. This research is helpful to the researchers developing optimal algorithms of cloth care systems.