폼페병의 진단 및 치료효과 확인을 위한 초고성능 액체크로마토그래피-탠덤질량분석기를 이용한 소변 포도당 사당류(Glc4) 측정법의 평가

남영원,이경훈,전선희,박경운,송상훈,박형두,송정한 대한진단검사의학회 2015 Laboratory Medicine Online Vol.5 No.4

We evaluated the urinary glucose tetrasaccharide (Glc4) assay using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The calibration curve was linear over a range of 5-500 μmol/L. Performance parameters such as intra- and inter-day imprecision CVs were 6.52-14.6% and 11.5-13.2%, respectively. The mean concentrations of urinary Glc4 in 27 normal controls and 3 pseudodeficiency patients were 1.5 and 12.1 mmol/mol creatinine, respectively. Urinary Glc4 concentration in a patient with Pompe disease was 171.3 mmol/mol creatinine, which decreased to 130.9 mmol/mol following enzyme replacement therapy. Based on our results, we suggest that the urinary Glc4 assay using UPLC-MS/MS can be a reliable diagnostic tool for identification of patients with Pompe disease. 본 연구에서는 초고성능 액체크로마토그래피-탠덤질량분석기를(UPLC-MS/MS) 이용한 소변 포도당 사당류(Glc4) 측정법에 대해 평가하였다. 직선성 평가에서 5-500 μmol/L의 범위에서 직선성을 보였고, 일내정밀도와 일간정밀도 변이계수는 각각 6.52-14.6%, 11.5-13.2%를 보였다. 27명의 정상대조군 및 3명의 가성결핍 환자들의 Glc4 평균값은 각각 1.5, 12.1 mmol/mol 크레아티닌이었다. 폼페병 환자의 진단 당시 Glc4 측정값은 171.3 mmol/mol 크레아티닌이었으며 효소대체치료를 받은 후에 130.9 mmol/mol 크레아티닌으로 감소하였다. 따라서 UPLC MS/MS를 이용한 소변 Glc4 측정은 폼페병의 진단에 보조적인 생물학적 표지자로 활용될 수 있을 것이다.

Evaluation of Matrix Effect in Body Fluid Chemistry on Roche Cobas 8000 c702 System

서종도,이지교,김승환,남영원,이준희,이경훈,송정한,송상훈 대한진단검사의학회 2021 Laboratory Medicine Online Vol.11 No.4

Background: Analysis of body fluids aids in diagnosis and monitoring disease. However, only a few testing platforms and reagents have been validated for a range of body fluids or analytes. In this study, we evaluated a testing system, which has been approved for blood samples, in analyzing body fluid specimens upon matrix mixing. Methods: Serum and body fluid samples, including cerebrospinal fluid (CSF), ascites, pleural fluid, amniotic fluid, and synovial fluid, were mixed, then the matrix effect and linearity for major analytes, namely amylase, chloride, glucose, LDH, and protein were evaluated (N = 30 serum-body fluid pairs) on the Cobas 8000 c702. The obtained data was compared with that of open reagents evaluated on the Architect c16000. Results: For all analyte-body fluid pairs, the mean percent recovery ranged from 98.4% to 101.7%, and this was within the acceptable range for matrix effect. In the linearity test, maximum non-linearity for each analyte-body fluid pair ranged from -5.0% to +4.2%. In interference test, proteins showed positive hemolytic, icteric, and lipemic interference in CSF and hemolytic interference in amniotic fluid. There was no significant interference in the other analyte-body fluid pairs. Results were highly correlated between the Cobas 8000 c702 and the Architect c16000 system. Conclusions: Our findings revealed that the matrix effect of major analytes in body fluid specimens can be excluded and they also validated the linearity of the analytes in the body fluid specimens. Therefore, reagents specified for blood samples can be readily adopted for the analysis of body fluids.

전치부 개교합을 가진 골격성 제 Ⅲ급 부정교합 환자에 있어서 하악지 시상분할 골절단술후 술후 안정성에 관한 평가

임재석,권종진,장현석,손형민,남영원,최철민 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.6

The purpose of this study is to assess the postoperative stability and the tendency of relapse after both sagittal split ramus osteotomy in skeletal class III with anterior open bite. This retrospective study was based on the examination of 15 patients, 6 males and 9 females, with a mean age 23.1 years. The patients were divided into two groups based on the amount of preoperative overbite(group I:<1mm, group II:≥1mm). Serial lateral cephalometric radiographs were taken within 2 weeks preoperatively, within 1 week postoperatively and at a follow up period that ranged from 7 to 24 months postoperatively. All cephalometric radiographs were traced and digitized using the Quick ceph image Pro for analysis. And then, horizontal and vertical changes of reference points(B point, Pogonion, L1) were assessed by the linear measurements in millimeters on both axes. The results were as follows : 1 Mean horizontal relapses were 0.67±0.93mm (11%) at B point (P<.05) and 0.81±1.01mm (13.2 %) at Pogonion (P<.01). 2, Mean vertical relapses were 0.63±1.62mm (33.3%) at B point and 0.53±1.95mm (31.4%) at Pogonion (P>.05) 3. The mean angular change of occlusal plane to SN was 0.24±1.19 (P>.05) and that of mandibular plane to SN was 1.03±1.85 (P<.05). 4. There was correlation between the extent of preoperative overbite and the postsurgical relapse at B point and Pogonion. 5. There was no correlation between the amount of surgically produced horizontal movement and the postoperative horizontal relapse at B point.

임상화학 분야 정량검사 결과의 단위 및 자릿수 보고 국내현황 고찰

조주영,정태동,문수영,정재우,남영원,이상국,임진숙,조은정,최현정,한민제,김솔잎 대한진단검사의학회 2022 Laboratory Medicine Online Vol.12 No.4

Background: The units and unit sizes of quantitative test results vary between laboratories and have not yet been standardized or harmonized in most cases. This study aimed to investigate the current status of units and unit sizes of clinical chemistry in Korea and propose standardized ones. Methods: The Korean Association of External Quality Assessment Service and College of American Pathologists survey data and textbooks (Tietz 6th ed. and Henry 24th ed.) were reviewed. Members of the Korean Society of Clinical Chemistry were surveyed on 99 items using an online sur vey platform, and a total of 99 institutions responded. For some items, simulations were conducted for standardized unit size proposals. Results: The units were consistent in all four references for a total of 54 items (54.5%), whereas, the unit sizes were consistent for 28 items (28.3%). In the questionnaire, 93 items (93.9%) accounted for more than 80% of institutions utilizing the most frequently used unit but only 30 items (30.3%) for unit sizes. As a result of the simulation for the number of digits proposal, it was found that the number of digits currently in use was acceptable for all three participating laboratories. Conclusions: To the best of our knowledge, this is the first study to investigate the current situation and simulation of the units and unit sizes in clinical chemistry in Korea through a literature review. These data are expected to be used as a basis for the setting of units and unit sizes for reporting quantitative test results in the future.

증례 : Morgagni 열공 Hernia 의 1 임사 예

이성재 ( S. J. Lee ),김종승 ( C. S. Kim ),남영원 ( Y. Y. Nam ) 대한내과학회 1968 대한내과학회지 Vol.11 No.6

The right pleural cavity and lung are involvd by direcet extension from the liver in 10 to 20 percent of patients with liver abscess, pulmonary amebiasis is diagnosed by typical chocolate like sputum X-Ray findings and history of having had amebic dysenter

악골의 낭종에 관한 임상적 연구

임재석(Jae Suk Rim),장현석(Hyon Seok Jang),손형민(Hyoung Min Son),남영원(Young won Nam) 대한구강악안면외과학회 2000 대한구강악안면외과학회지 Vol.26 No.3

The purpose of this study is to find the histopathological pattern of cysts. We reviewed the hospital chart, out-patient chart, roentgenogram, histopathologic report and operation report of 152 patients who had been diagnosed as cyst and treated at the department of Oral and Maxillofacial Surgery of Korea university hospital between Jan. 1, 1995 and Dec. 31, 1998. And then we studied clinically with regard to pathological classification, age and sex distribution, anatomical distribution and so on. The results were as follows: 1. In pathologic classification, radicular cyst (97cases, 64%), dentigerous cyst (35cases, 23%), odontogenic keratocyst (8cases, 5.3%) were dominant among cases of cyst. 2. The pattern of age distribution in cases of radicular cyst, dentigerous cyst and odontogenic keratocyst was similar to that found in previous studies. The peak incidence was in the second decade (27%) and third decade (29%) in overall cases. 3. The male-to-female ratio was 1.9:1. 4. Radicular cyst occured most frequently in the maxillary incisor teeth area, dentigerous cyst in mandibular wisdom teeth area, and odontogenic keratocyst in mandibular molar area.