알코올을 처리한 HepG2 세포에서 참죽나무 잎 추출물의 세포 보호 및 항산화 효과

김현정,조수연,김정봉,김헌웅,최정숙,장환희,Kim, Hyun-Jeong,Cho, Su-Yeon,Kim, Jung-Bong,Kim, Heon-Woong,Choe, Jeong-Sook,Jang, Hwan-Hee 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.4

All the parts of the Cedrela sinensis A. Juss., including the seeds, roots, and leaves, have been known to exert medicinal effects. The C. sinensis and its major compound, quercetin, were previously reported to exhibit the anti-inflammatory and anti-oxidative activities. However, the hepatoprotective effects of the C. sinensis leaves against the alcohol-induced oxidative stress in the HepG2 cells have not been studied. In this study, we investigated the antioxidant activities and analyzed the flavonoid contents of the C. sinensis-leaf extract (CE). The total flavonoid contents of the CE is 1,874.5 mg/100 g dry weight (DW), while the total quercetin 3-O-rhamnoside (quercitrin) contents, which was identified as the major flavonol in the CE, is 1,456.0 mg/100 g DW. In the ethanol-stimulated HepG2 cells, the CE effectively prevented the cytotoxic effect and increased the gene expression of the antioxidant enzymes, such as the heme oxygenase-1 (HO-1) and the glutathion peroxide (GPx). The level of the reactive oxygen species (ROS) production was significantly decreased in the CE-treated HepG2 cells. In conclusion, the C. sinensis extract suppressed the alcohol-induced oxidative stress in the HepG2 cells via the induced GPx and HO-1 gene expressions. It is expected the CE positive effects will likely be attributed to the flavonoids, like the quercetin, within the CE.

유아교사 요구에 기초한 장애유아 통합교육 연수프로그램이 교사효능감과 만족도에 미치는 효과

김현정(Kim Hyun-jeong),홍혜경(Hong Haekyung) 한국열린유아교육학회 2007 열린유아교육연구 Vol.12 No.6

본 연구는 유아교사 요구에 기초한 장애유아 통합교육 연수프로그램이 통합교육 교사효능감과 만족도를 알아보는데 있다. 연구의 대상은 연구자가 개발한 장애유아 통합교육 교사 연수프로그램에 참여한 실험집단 22명과 정부기관에서 위탁 통합교육 일반연수에 참여한 통제집단 교사 22명으로, 현재 유아교육현장에서 장애유아를 담당하고 있는 일반유아교사이다. 본 연구의 도구로는 통합교육 교사효능감을 측정하기 위하여 배재정(2005)이 번역하여 사용한 교사효능감의 도구와 김정주(2005), 조광순과 황인갑(2003), 조운주(2004)가 사용한 연수프로그램에 대한 교사의 만족도를 사용하였다. 자료분석은 실험집단과 통제집단간 통합교육 교사효능감의 차이를 비교하기 위하여 통합교육 교사효능감 사전검사 점수를 공변인으로 하고, 사후검사 점수를 종속변인으로 한 ANCOVA를 실시하였으며, 프로그램 만족도의 두 집단 간 차이를 비교하기 위해 t검증을 하였다. 장애유아 통합교육 유아교사 연수프로그램의 효과를 검증한 결과, 통합교육 교사효능감은 실험집단이 통제집단보다 매우 유의하게 높은 것으로 밝혀졌다. 교사효능감의 두 가지 하위영역인 일반적 교사효능감, 개인적 교사효능감에서 있어서도 실험집단이 통제집단보다 매우 유의하게 높은 것으로 나타났다. 또한 본 연구에 참여한 실험집단 교사들은 통제집단 교사들보다 연수에 대한 만족도에서 점수가 높은 것으로 나타났으며, 이러한 차이는 두 집단 간에 통계적으로 유의한 차이가 나타났다. This study examines the effects of a training program of inclusive education for young children with disabilities based on childcare teachers' demands on the teachers' efficacy and satisfaction. The subjects of this study were ordinary childcare teachers at early childhood education institutions executing inclusive education for ordinary and disabled young children. We applied a training program of inclusive education for young children with disabilities to the experimental group of 22 ordinary childcare teachers, and a training program of inclusive education entrusted by institutions to the control group of 22 teachers. In this study, inclusive education teachers' efficacy was measured with the teacher efficacy scale translated by Bae Jae-jeong (2005) and teachers' satisfaction was measured with the scale of satisfaction with training programs used by Kim Jeong-joo (2005), Cho Gwang-soon and Hwang In-gap (2003) and Cho Woon-joo (2004). In data analysis, the difference in inclusive education teachers' efficacy between the experimental group and the control group was analyzed through ANCOVA with the score of pretest on inclusive education teachers' efficacy as a covariate and the score of posttest as a dependent variable. In addition, the difference in satisfaction with programs between the two groups was examined by t-test. According to the results of testing the effect of the training program of inclusive education for young children with disabilities, the experimental group showed significantly higher teacher efficacy than the control group. In the two sub-areas of teacher efficiency - general teacher efficacy and personal teacher efficacy - the experimental group was significantly higher than the control group. In addition, the teachers in the experimental group showed a higher score of satisfaction than those in the control group, and the difference was statistically significant.

차잎의 초기 성장 시기 동안 lipoxygenase 활성 및 휘발성 향기성분의 변화

김현정,류성권,노진철,이상준,박승국,Kim, Hyun-Jeong,Ryu, Sung-Kwon,Roh, Jin-Chul,Lee, Sang-Jun,Park, Seung-Kook 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.1

Lipoxygenase는 녹차의 전체적인 품질에 영향을 미치는 "신선하고 풋풋한 풀 향기(green odor)" 향기특성을 가지는 $C_6-alcoho$과 $C_6-aldehyde$ 등 $C_6$화합물을 생성시키는데 관여하는 효소로서 잘 알려져 있다. 본 연구에서는 녹차잎의 초기 성장기간인 4뭘 4에서 15일까지 12일 동안에 매일 채엽한 녹차잎의 lipoxygenase 활성을 측정하고, 이에 따른 $C_6$화합물 및 terpene 등 휘발성 향기성분을 분석하여 수확 날짜에 따른 효소 활성과 녹차잎의 중요한 휘발성 향기성분의 변화를 살펴보았다. 효소활성의 측정은 linoleic acid를 기질로 한 흡광도 측정방법을 사용하였고, 휘발성 향기성분은 Solid Phase MicroExtraction (SPME) 방법으로 휘발성 향기성분을 포집한 후에 GC또는 GC-MS를 이용해 분석하였다. 실험 결과, 초기 녹차 잎 성장기간 동안 lipoxygenase 활성과 $C_6$화합물의 함량은 서로 비례하여 증가 또는 감소하였고, 이러한 변화는 당일의 온도변화와 밀접한 관계가 있는 것으로 나타났다. 즉, 온도가 증가함에 따라서 향기성분의 양도 증가하였으며, 너무 이른 시기에 채엽한 시료는 향기 성분이 매우 적은 것으로 나타났다. 본 연구로부터 "신선한 풀향기"와 "향긋한 꽃향기 (floral)" 등 녹차향 품질에 중요한 영향을 미치는 향 화합물들이 녹차의 초기 채엽기간 중에 어떻게 변화하는지에 대해서 확인 할 수 있었으며, 향기품질이 우수한 녹차를 제조하기 위해서는 생엽 성장 기간동안의 향기성분변화를 이해하는 것이 중요하다는 것을 확인하였다. Lipoxygenase is the enzyme responsible for the formation of $C_6$-alcohols and $C_6$-aldehydes ($C_6$-compounds), which are well blown contributors to various types of 'green odor' In green tea. Changes in lipoxygenase activity and volatile compounds of green tea leaves were monitored daily during early growing season. The enzyme activity was spectrophotometrically measured using linoleic acid as a substrate. The volatile compounds were extracted through Solid Phase Micro-Extraction, and were subjected to GC and GC-MS analyses. Results showed that lipoxygenase activity and levels of $C_6$-compounds concomitantly increased or decreased during the early growing season, probably caused by the fluctuation in the daily temperature; increase in temperature led to the increase in enzyme activities and $C_6$-compound levels, whereas leaves plucked too early had low volatile compound levels. In this study, optimum plucking time of tea leaves for the production of high quality green tea with a wellbalanced aroma was determined.

Metal Powder에 따른 증기화 증폭 시트의 개발을 통한 열 중량 분석 및 고출력 전자빔의 가공 특성 분석

김현정,정성택,이주형,백승엽,Kim, Hyun-Jeong,Jung, Sung-Taek,Lee, Joo-Hyung,Baek, Seung-Yub 한국금형공학회 2020 한국금형공학회지 Vol.14 No.1

An electron beam was used to mainly utilize for polishing, finishing, welding, a lithography process, etc. Due to the high technical level of difficulty of high-power density electron beam, it is difficult to secure related technologies. In this study, research was carried out to improve the machinability by developing the vaporized amplification sheets to realize the electron beam drilling technology. Their vaporized amplification sheets were analyzed by using the measurement of chemical and composition, which is such as TGA, SEM. We analyzed micro-hole processing using a microscope. Also, the thermal characteristics of vaporized amplification sheets are highly significant for applying to high-power density electron beam technique. So, we finished the vaporized amplification sheets according to the process conditions and analyzed it according to the machining conditions of the electron beam. It was confirmed that the effect on the experimental results differs depending on the influence of the metal powder contained in the developed material.

치과에서 발생하는 국소마취에 의한 신경손상

김현정,Kim, Hyun Jeong 대한치과마취과학회 2014 Journal of Dental Anesthesia and Pain Medicine Vol.14 No.2

Local anesthesia known as the safe and essential procedure to control pain in dentistry may cause sensory changes such as paresthesia or altered taste at the affected sites after even successful local anesthesia. Although the prognosis of the nerve injuries after local anesthesia is favorable, it might cause prolonged problems such as dysesthesia. The lingual nerve is a single fascicle at the level of the lingual among 1/3 of patients and more movable during regeneration compared to the inferior alveolar nerve after the injury. As a result, the lingual nerve is more vulnerable and has poorer outcomes. More vigilant clinical considerations are required to the lingual nerve injury after local anesthesia. Generally, more than 80% of cases are spontaneously resolved within 2 weeks after the local anesthesia even without any specific treatment. However, the patient having long lasting abnormal sensations more than 2 weeks needs specialists' care for further assessment. In case of dysesthesia which is a symptom of neuropathic pain, immediate referral to specialists is mandatory. The exact mechanism, how to prevent its occurrence, or specific treatments of the nerve injury related to the local anesthesia have not been elucidated. To prepare clinical or medicolegal problems, many cautious considerations are given to the patients who complain sensory changes after local anesthesia.

평면전극구조를 갖는 바이올로진 기반의 일체형 전기변색소자

김현정,이상래,최진희,나윤채,Kim, Hyun-Jeong,Lee, Sang-Rae,Choi, Jin-Hee,Nah, Yoon-Chae 한국분말야금학회 2020 한국분말재료학회지 (KPMI) Vol.27 No.1

Recently, electrochromic devices (ECDs) have gathered increasing attention owing to their high color contrast and memory effect, which make them highly applicable to smart windows, auto-dimming mirrors, sensors, etc. Traditional ECDs have a sandwich structure that contains an electrochromic layer between two ITO substrates. These sandwich-type devices are usually fabricated through the lamination of two electrodes and followed by the injection of a liquid electrolyte in the inner space. However, this process is sometimes complex and time consuming. In this study, we fabricated ECDs with a lateral electrode structure that uses only an ITO substrate and an all-in-one electrochromic gel, which is a mixture of electrolyte and electrochromic material. Furthermore, we investigated the EC properties of the lateral-type device by comparing it with a sandwich-type device. The lateral-type ECD shows strong blue absorption as the applied voltage increases and has a competitive coloration efficiency compared to the sandwich-type device.

영지버섯으로부터 homocysteine methyltransferase 를 암호화 하는 metE 유전자의 클로닝 및 E. coli 에서의 발현

김현정,박동철,이갑득,이별라,이갑랑 ( Hyun Jeong Kim,Dong Chul Park,Kap Duk Lee,Byul La Lee,Kap Rang Lee ) 한국균학회 1993 韓國菌學會誌 Vol.21 No.4

VAS(Visual Analogue Scale), TTO(Time Trade-Off), EQ-5D(EuroQol-5 Dimension) 방법을 이용한 중증위암의 효용가중치 측정

김현정,이진이,이의경,Kim, Hyun-Jeong,Rhee, Jinn-Ie,Lee, Eui-Kyung 대한약학회 2008 약학회지 Vol.52 No.3

Stomach cancer shows the highest incidence among cancer patients in Korea and it deteriorates the patients' health-related quality of life considerably. This study measured utility weights of severe stomach cancer symptoms using VAS (Visual Analogue Scale), TTO (Time-trade Off) and EQ-5D (EuroQol-5 Dimension) methods. A survey questionnaire was developed to describe the symptoms of severe stomach cancer comprehensively and concisely using VAS, TTO and EQ-5D. A face-to-face interview was conducted for 21 cancer inpatients in a hospital and 25 non-patients. The mean utility weight is 0.498 for VAS, 0.375 for EQ-5D and 0.300 for TTO. Using VAS or TTO methods, there were no statistically significant differences between patients and non-patients while there was significant difference in EQ-5D. The utility weight with VAS and EQ-5D has shown similar trend - higher score in patient group, male and aged while it has opposite trend with TTO.

벤조피렌을 투여한 생쥐의 간 조직에서의 홍삼분말의 항산화 효과

김현정,이지원,지영주,유미희,박정현,이기동,이인선,Kim, Hyun-Jeong,Lee, Ji-Won,Ji, Young-Ju,Yu, Me-Hei,Park, Jung-Hyun,Lee, Ki-Dong,Lee, In-Seon 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.5

홍삼분말이 $B(\alpha)P$의 투여에 의한 간 독성이 유발된 생쥐에서의 글루타치온 및 과산화지질 함량, 항산화효소 활성에 미치는 영향을 살펴보았다. $B(\alpha)P$ 투여에 의해 증가된 혈청내 ALT와 AST의 활성이 홍삼분말 전 처리군에서는 감소하였다. 간 조직중의 글루타치온 함량은 $B(\alpha)P$ 단독군에서는 감소되었다가 홍삼분말 투여시 유의적인 증가를 보였고, 지질과산화물 함량은 $B(\alpha)P$ 투여시 증가되었다가 홍삼분말의 투여시 유의적으로 감소되었다. 간 조직중의 SOD, catalase 그리고 GSH-Px의 활성도 $B(\alpha)P$ 투여로 유의적으로 증가되었다가, 홍삼분말의 투여로 이들 활성이 유의적으로 감소하였다. 반면, GST 활성은 $B(\alpha)P$ 단독군에서는 감소되었다가 홍삼분말 투여시 유의적인 증가를 보였다. 이상의 결과로 홍삼분말은 항산화효소의 활성에 의한 $B(\alpha)P$에 의한 간 손상에 대한 보호효과를 가지는 것으로 사료된다. The effects of red ginseng powder on hepatotoxicity in $benzo(\alpha)pyrene\;[B(\alpha)P]$-treated mice were investigated. Male ICR mice were pretreated with red ginseng powder (50 or 100 mg/kg/day, for 5 days, intraperitoneally) before treatment with $B(\alpha)P$ (0.5 mg/kg, i.p, single dose). The ability of red ginseng powder to protect against oxidative damage to the mouse liver was examined by determining the level of lipid peroxide, glutathione, and the antioxidant enzyme activities. The glutathione content depleted by $B(\alpha)P$ were significantly increased by red ginseng powder, but elevation of lipid peroxide content induced by $B(\alpha)P$ was decreased by red ginseng powder. The increased activities of superoxide dismutase, catalase and glutathione peroxidase after $B(\alpha)P$-treatment were decreased by the treatment of red ginseng Powder; however, glutathione S-transferase activity depleted by $B(\alpha)P$ were significantly increased. These results suggest that red ginseng powder can protect against $B(\alpha)P$ intoxification through its antioxidant properties.

Application of NORM to the Multiple Imputation for Multivariate Missing Data

김현정,문승호,신재경,Kim, Hyun-Jeong,Moon, Sung-Ho,Shin, Jae-Kyoung 한국데이터정보과학회 2002 한국데이터정보과학회지 Vol.13 No.2

The statistical analysis of incomplete data sometimes requires handling of incomplete observations. Towards this end, each case with some missing values generally should be deleted, namely, resulting in only use of non-missing cases. EM algorithm(Dempster et al., 1977) which involves prediction and estimation steps is a general method among others. In this article, we use the free software NORM developed for multiple imputation, which uses DA(Data Augmentation) algorithm in its imputation, and evaluate its efficiency through a numerical example.