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알킬화제에 의한 DNA 단사절단 , 절제회복 및 자매염색분체 교환에 미치는 Poly ( ADP - ribose ) polymerase 저해제의 상승효과
김철근,박종군,박상대 ( Chul Geun Kim,Jong Kun Park,Sang Dai Park ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.2
Benzamide and 3-aminobenzamide, potent inhibitors of poly (ADP-ribose) polymerase, increased MMS or MNNG-induced DNA strand breaks and significantly retarded the rejoining of strand breaks in CHO cells. These inhibitors also synergistically enhanced the frequencies of unscheduled DNA synthesis (UDS) and sister chromatid exchanges (SCE) induced by MNNG. Amounts of UDS and strand breaks induced by the combined treatment with MMS and MNNG were found to be additive but these amount were enhanced in the presence of inhibitors. These results suggest that poly (ADP-ribose) functions a regulatory role in the repair of DNA damage by virtue of stabilizing chromatin structure whenever strand breaks occur in DNA.
김철근,Hyen Seok Heo,Ju Hyun Kim,Young Jin Lee,Sung-Hyun Kim,조윤신 한국분자세포생물학회 2005 Molecules and cells Vol.20 No.1
Murine erythroleukemia (MEL) cells are widely used to study erythroid differentiation thanks to their ability to terminally differentiate in vitro in response to chemical induction. At the molecular level, not much is known of their terminal differentiation apart from activation of adult-type globin gene expression. We examined changes in gene expression during the terminal differentiation of these cells using microarray-based technology. We identified 180 genes whose expression changed significantly during differentiation. The microarray data were analyzed by hierarchical and k-means clustering and confirmed by semi-quantitative RT-PCR. We identified several genes including H1f0, Bnip3, Mgl2, ST7L, and Cbll1 that could be useful markers for erythropoiesis. These genetic markers should be a valuable resource both as potential regulators in functional studies of erythroid differentiation, and as straightforward cell type markers.
김철근,박종군,박상대,Kim, Chul-Geun,Park, Jong-Kun,Park, Sang-Dai 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.2
CHO 세포에 있어서 강력한 poly(ADP-ribose) polymerase 저해제인 benzamide와 3-aminobenzmide는 MMS와 MNNG에 의한 DNA 단사절단을 증가시켰으며 단사절단의 재결합을 유의성 있게 억제하였다. 이들 저해제는 또한 MNNG에 의한 비주기성 DNA 합성(UDS)과 자매염색분체 교환(SCE)에 상승 효과를 나타내었다. MMS와 MNNG의 복합처리에 의한 UDS와 단사절단은 상가효과를 보이나 저해제 처리에 의해 이의 효과는 상승되었다. 이들 결과로 보아 poly(ADP-ribose)는 DNA에 단사절단이 일어날때마다 염색사구초를 안정화 시킴으로서 DNA 손상에 대한 회복에 조절작용을 나타낸다고 사료된다. Benzamide and 3-aminobenzamide, potent inhibitors of poly (ADP-ribose) polymerase, increased MMS or MNNG-induced DNA strand breaks and significantly retarded the rejoining of strand breaks in CHO cells. These inhibitors also synergistically enhanced the frequencies of unscheduled DNA synthesis (UDS) and sister chromatid exchanges (SCE) induced by MNNG. Amounts of UDS and strand breaks induced by the combined treatment with MMS and MNNG were found to be additive but these amount were enhanced in the presence of inhibitors. These results suggest that poly (ADP-ribose) functions a regulatory role in the repair of DNA damage by virtue of stabilizing chromatin structure whenever strand breaks occur in DNA.
인간 지방조직유래 줄기세포의 PLCL/피브린 복합지지체에서 연골세포로의 분화
김상희 ( Sang Hee Kim ),정영미 ( Young Mee Jung ),김상헌 ( Sang Heon Kim ),이종원 ( Jong Won Rhie ),김영하 ( Young Ha Kim ),김철근 ( Chul Geun Kim ),김수현 ( Soo Hyun Kim ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
In cartilage tissue engineering, choosing on an appropriate cell source and a scaffold for cartilaginous tissue formation seriously influences the clinical application of an engineered cartilage. The purpose of this study is to investigate chondrogenic differentiation of human adipose tissue-derived stromal cells(hASCs) by hybridizing of fibrin gels for mimicking 3-dimensional environments of native cartilage and a poly(L-lactide-co-ε-caprolactone)(PLCL) scaffold. PLCL scaffolds with 85% porosity and 300-500 ?m pore size were fabricated by a gel-pressing method. For examining chondrogenic differentiation of hASCs, cells were mixed with 0.5% fibrin solutions and subsequently they were seeded onto PLCL scaffolds. After that, cell-scaffold constructs were implanted subcutaneously in nude mice for up to 8 weeks. Specimens were harvested after 8 weeks and analyzed. From in vivo studies, the constructs used with fibrin-PLCL hybrid scaffolds showed more depositions of cartilage-specific ECM components, such as GAGs and collagen type II, as compared to the constructs used with PLCL scaffolds alone. Furthermore, the constructs seeded with hASCs that were induced to chondrogenesis in vitro prior to implantation improved cartilage matrix deposition as compare to the constructs seeded with non-induced hASCs. These results indicated that the use of the fibrin gels-PLCL hybrid scaffold could enhance chondrogenic differentiation of hASCs and cartilaginous tissue formation. Also, the induction for chondrogenesis of hASCs prior to implantation in vitro could affect cartilaginous tissue formation in vivo. In conclusion, the hybrid system which was fabricated with the inoculation of ASCs and fibrin gels onto a PLCL scaffold could be a meaningful system in cartilage tissue engineering.