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김철근,박종군,박상대,Kim, Chul-Geun,Park, Jong-Kun,Park, Sang-Dai 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.2
CHO 세포에 있어서 강력한 poly(ADP-ribose) polymerase 저해제인 benzamide와 3-aminobenzmide는 MMS와 MNNG에 의한 DNA 단사절단을 증가시켰으며 단사절단의 재결합을 유의성 있게 억제하였다. 이들 저해제는 또한 MNNG에 의한 비주기성 DNA 합성(UDS)과 자매염색분체 교환(SCE)에 상승 효과를 나타내었다. MMS와 MNNG의 복합처리에 의한 UDS와 단사절단은 상가효과를 보이나 저해제 처리에 의해 이의 효과는 상승되었다. 이들 결과로 보아 poly(ADP-ribose)는 DNA에 단사절단이 일어날때마다 염색사구초를 안정화 시킴으로서 DNA 손상에 대한 회복에 조절작용을 나타낸다고 사료된다. Benzamide and 3-aminobenzamide, potent inhibitors of poly (ADP-ribose) polymerase, increased MMS or MNNG-induced DNA strand breaks and significantly retarded the rejoining of strand breaks in CHO cells. These inhibitors also synergistically enhanced the frequencies of unscheduled DNA synthesis (UDS) and sister chromatid exchanges (SCE) induced by MNNG. Amounts of UDS and strand breaks induced by the combined treatment with MMS and MNNG were found to be additive but these amount were enhanced in the presence of inhibitors. These results suggest that poly (ADP-ribose) functions a regulatory role in the repair of DNA damage by virtue of stabilizing chromatin structure whenever strand breaks occur in DNA.
알킬화제에 의한 DNA 단사절단 , 절제회복 및 자매염색분체 교환에 미치는 Poly ( ADP - ribose ) polymerase 저해제의 상승효과
김철근,박종군,박상대 ( Chul Geun Kim,Jong Kun Park,Sang Dai Park ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.2
Benzamide and 3-aminobenzamide, potent inhibitors of poly (ADP-ribose) polymerase, increased MMS or MNNG-induced DNA strand breaks and significantly retarded the rejoining of strand breaks in CHO cells. These inhibitors also synergistically enhanced the frequencies of unscheduled DNA synthesis (UDS) and sister chromatid exchanges (SCE) induced by MNNG. Amounts of UDS and strand breaks induced by the combined treatment with MMS and MNNG were found to be additive but these amount were enhanced in the presence of inhibitors. These results suggest that poly (ADP-ribose) functions a regulatory role in the repair of DNA damage by virtue of stabilizing chromatin structure whenever strand breaks occur in DNA.
인간 지방조직유래 줄기세포의 PLCL/피브린 복합지지체에서 연골세포로의 분화
김상희 ( Sang Hee Kim ),정영미 ( Young Mee Jung ),김상헌 ( Sang Heon Kim ),이종원 ( Jong Won Rhie ),김영하 ( Young Ha Kim ),김철근 ( Chul Geun Kim ),김수현 ( Soo Hyun Kim ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
In cartilage tissue engineering, choosing on an appropriate cell source and a scaffold for cartilaginous tissue formation seriously influences the clinical application of an engineered cartilage. The purpose of this study is to investigate chondrogenic differentiation of human adipose tissue-derived stromal cells(hASCs) by hybridizing of fibrin gels for mimicking 3-dimensional environments of native cartilage and a poly(L-lactide-co-ε-caprolactone)(PLCL) scaffold. PLCL scaffolds with 85% porosity and 300-500 ?m pore size were fabricated by a gel-pressing method. For examining chondrogenic differentiation of hASCs, cells were mixed with 0.5% fibrin solutions and subsequently they were seeded onto PLCL scaffolds. After that, cell-scaffold constructs were implanted subcutaneously in nude mice for up to 8 weeks. Specimens were harvested after 8 weeks and analyzed. From in vivo studies, the constructs used with fibrin-PLCL hybrid scaffolds showed more depositions of cartilage-specific ECM components, such as GAGs and collagen type II, as compared to the constructs used with PLCL scaffolds alone. Furthermore, the constructs seeded with hASCs that were induced to chondrogenesis in vitro prior to implantation improved cartilage matrix deposition as compare to the constructs seeded with non-induced hASCs. These results indicated that the use of the fibrin gels-PLCL hybrid scaffold could enhance chondrogenic differentiation of hASCs and cartilaginous tissue formation. Also, the induction for chondrogenesis of hASCs prior to implantation in vitro could affect cartilaginous tissue formation in vivo. In conclusion, the hybrid system which was fabricated with the inoculation of ASCs and fibrin gels onto a PLCL scaffold could be a meaningful system in cartilage tissue engineering.
자기부상열차 시험용 1/2차량 대차제작과 주행성능 실험
이남진(Lee Nam-Jin),한형석(Han Hyungsuk),이원상(Lee Wan-Sang),김철근(Kim Chul-Geun) 한국철도학회 2009 한국철도학회 학술발표대회논문집 Vol.2009 No.5월
Maglev vehicle has two kinds of suspension system such as a secondary suspension with air-spring and a primary suspension as electromagnetic suspension which composed of electromagnet, magnet driver, controller and sensors. The interaction between each dynamic component of vehicle and track effects the stability and running performance. To achieve the specified performance of vehicle, many various approaches of research were tried, then as the result of these efforts, the first commercial operating with Maglev will start soon. The bogie for revenue service from 2012 has some significant modifications compared to the previous one, and to verify the changes the half prototype vehicle was manufactured and took the running performance test. In this report, we will introduce the stage of manufacturing and report results of dynamic performance tests to verify new concept of bogie mechanism.
LQ제어 기법을 활용한 자기부상열차 부상제어기 설계에 관한 연구
이남진(Lee Nam-Jin),한형석(Han Hyungsuk),양방섭(Yang Bang-Sup),김철근(Kim Chul-Geun) 한국철도학회 2007 한국철도학회 학술발표대회논문집 Vol.- No.-
The levitation system of Maglev is composed with electro-magnet, power supplier, controller and sensor. The complex interactions between above subcomponents define the characteristics of electromagnetic suspension of the vehicle. In this study, to understand the influence of controller on the running performance of Maglev, the new controller based on LQ theory will be designed and be simulated with simplified vehicle model. Then the influence of controller on the characteristics of electromagnetic suspension will be reviewed through comparison with existing control algorithm of our prototype vehicle.
갈색 지방 조직의 선택적 절제가 가능한 형질 전환 비만 생쥐의 제조
김철근,최웅환,지정훈,김수경,김범수,김대환 漢陽大學校 自然科學硏究所 2000 自然科學論文集 Vol.19 No.-
갈색지방세포는 체내의 잉여 에너지원을 열로 발산시킴으로써 체내 양분 균형과 체중을 유지한다. 갈색지방세포의 이러한 기능을 제한적 발열작용 (regulated thermogenesis)이라 한다. 본 연구에서는, 특정 발생단계나 물리 ·생리적 조건에서 갈색지방조직의 선택적인 유전적 제거를 위해 갈색지방세포 특이적으로 발현하는 UCP-1 유전자의 특정 조절 부위에 의해 발현되는 HSV-TK 유전자를 가지는 형질 전환 생쥐를 만들었다. 이 생쥐에 ganciclovir를 처리하면 특정 시간, 조건에서 갈색지방세포를 선택적으로 제거할 수 있으므로 갈색 지방세포에 의한 체중조절의 기전을 이해할 수 있고, 체중에 영향을 미치는 다른 요인도 연구할 수 있을 것이다. Brown adipose tissue maintains nutrient balance and body weight by wasting the excess energy materials as heat. This function of the brown adipocyte is called regulated thermogenesis. In order to genetically remove the brown adipose tissue at specific developmental stages or physical and physiological conditions based on a conditional genetic tissue ablation method, we have made transgenic mouse carrying HSV-TK gene which is expressed by the brown adipocyte specific regulatory region of UCP-1 gene. By treating this transgenic mice with ganciclovir at specific times and conditions, we can monitor the effect of brown adipose tissue on the body weight regulation and this system is also expected to be used on the study of other factors affecting the body weight.