신육성 품종인 루비에스(Ruby S; Malus pumila Mill.) 사과껍질의 항산화, 건강 기능성 및 미용식품 활성

이은호(Eun-Ho Lee),김예진(Ye-Jin Kim),권순일(Soon-Il Kwon),김정희(Jeong-Hee Kim),강인규(In-Kyu Kang),정희영(Hee-Young Jung),박경일(Kyeung-Il Park),조영제(Young-Je Cho) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.11

식육성 품종 루비에스 껍질의 water와 ethanol 추출물들의 항산화, 건강 기능성 및 미용식품 활성을 살펴보았다. 신육성 품종 루비에스 껍질로부터 water와 ethanol로 추출하였을 때 각각 5.29, 8.76 mg/g의 비교적 높은 phenolic 함량을 나타내었다. 루비에스 껍질 추출물의 antioxidant protection factor는 water와 ethanol 추출물 100 ㎍/mL phenolics 농도에서 각각 1.87, 1.77 PF를 나타내었으며, TBARS는 100 ㎍/mL phenolics 농도에서 water 추출물은 80.17%, ethanol 추출물은 38.72%의 항산화능을 나타내어, 루비에스 사과 껍질 추출물의 경우 노화방지를 위한 천연 항산화제로 활용 가능성이 매우 높은 것으로 판단하였다. 루비에스껍질 추출물의 항염증(hyaluronidase 저해) 효과는 water 및 ethanol 추출물 200 ㎍/mL phenolics 농도에서 각각 5.61, 31.87%의 저해 효과를 나타내었다. 통풍을 유발하는 xanthine oxidase 저해 효과는 ethanol 추출물에서만 100 ㎍/mL의 phenolic 농도에서 28.66%의 xanthine oxidase 저해 효과를 나타내어 대조구로 사용된 후지 품종에 비교해 상대적으로 높은 저해 효과를 나타내었다. 루비에스 껍질추출물의 α-amylase 저해 효과는 water 추출물과 ethanol 추출물 모두 비교적 낮은 저해 효과를 나타내었으나, α-glucosidase 활성저해의 경우 200 ㎍/mL의 phenolic 농도에서 water와 ethanol 추출물에서 각각 94.93%와 100.00%의 매우 높은 저해 효과를 나타내었다. 미백 효과를 측정하는 tyrosinase 저해 효과는 200 ㎍/mL의 phenolic 농도에서 ethanol 추출물에서만 17.50%의 저해 효과를 나타내었다. 주름 개선 효과를 나타내는 elastase 및 collagenase 저해 효과는 200 ㎍/mL phenolics 농도의 water 추출물에서 각각 6.70, 69.85%의 저해 효과를 나타내었고, ethanol 추출물에서 각각 41.95, 80.73%의 저해 효과를 나타내어 기능성 화장품 개발을 위한 소재로 활용 가능성이 매우 높은 것으로 판단하였다. 따라서 루비에스 껍질 추출물의 항산화, 항염증, 통풍 억제, 항당뇨, 미백 및 주름 개선 효과 및 항당뇨, 통풍 억제 효과를 확인하였고, 기존의 후지 품종과 비교해서 기능성이 더 우수함이 밝혀져 새로운 기능성 화장품 및 건강 기능성 식품 조성물로 이용이 가능할 것으로 판단되었다. In this study, the anti-oxidative, health functional, and beauty food activities of water and ethanol extracts from newly bred Ruby S apple (Malus pumila Mill.) peel. Peel of newly bred Ruby S apple was extracted using water and ethanol for extracting solvent. Water and ethanol extracts showed relatively high phenolic contents of 5.29 ㎎/g and 8.76 ㎎/g, respectively. Each water and ethanol extracts of Ruby S apple peel showed antioxidant protection factor values of 1.87 and 1.77 PF, respectively, and TBARS showed anti-oxidative effects of 80.17% and 38.76% at a phenolic concentration of 100 ㎍/mL. Therefore, extract of Ruby S apple peel can be considered as an anti-oxidative and anti-aging agent. The anti-inflammatory effect (hyaluronidase inhibition) of extract of Ruby S apple peel was 5.61% in water extract and 31.87% in ethanol extract both at a phenolic concentration of 200 ㎍/mL. Activity of xanthine oxidase, which causes gout, was inhibited by 28.66% by ethanol extract at 100 ㎍/mL, which was higher than other species used as a control, whereas there was no effect using water extract. Both water and ethanol extracts showed low α-amylase inhibitory effects, whereas α-glucosidase inhibitory activities were 94.93% and 100.00% at a phenolic concentration of 200 ㎍/mL, respectively. To measure whitening effect, tyrosinase inhibitory activity was measured, and only ethanol extract showed an inhibitory effect (17.50%). For anti-wrinkle effect, water extract showed 6.70% and 69.85% elastase and collagenase inhibitory activities, respectively, while ethanol extract showed 41.95% and 80.73% activities. These results show the high potential of Ruby S apple peel extracts as a cosmetic resource. Ruby S apple peel was identified to have various functions, including anti-oxidative, anti-inflammatory, gout preventative, whitening, anti-wrinkle, and anti-diabetic effects, which confirms that Ruby S apple peel is an outstanding species compared to other species. Therefore, Ruby S apple peel is a quality source of new functional cosmetics and can be viewed as a functional foods.

소세포폐암에서 Cisplatin 과 Etoposide 복합 화학요법 및 방사선 병합치료의 장기간 관찰결과 ( 최종보고 )

류혜영(Hyae Young Lew),최환석(Hwan Seok Choi),박지훈(Ji Hoon Park),김정희(Jeong Hee Kim),김시영(Si Young Kim),윤휘중(Hwi Joong Yoon),홍성언(Sung Eun Hong),조경삼(Kyung Sam Cho) 대한내과학회 1996 대한내과학회지 Vol.50 No.6

N/A Objectives: Small cell lung cancer is the one of solid tumors most responsive to chemotherapy and radiation therapy. Most commonly used combined therapeutic regimen was CAV(cyclophosphamide, adriamycin, vincristine), Reports of the cisplatin plus VP-16(VPP) combination as second line therapy following CAV suggested that VPP might be non- cross resistant with the CAV regimen. We evaluated the efficacy and toxicity of VPP combination therapy as a first line therapy for small cell lung cancer (SCLC) Methods: Forty-four patients with SCLC were treated with cisplatin (20mg/m2 i,v., X days) and etoposide (100mg/m2 i.v., X 3 days) every three weeks. In patients with limited disease, radiation to primary site was performed at 3rd or 4th cycle of VPP. Results: 1) Forty two patients were evaluated for response to VPP chemotherapy. The two patients were excluded because they died before evaluating response to chemotherapy. The overall response rate was 72%(CR 24%, PR 48%). The response rate of limited disease and extensive disease was 77% and 63% respectively. 2) The median overall survival time was 52 weeks. Survival rates of 1year, 3years, and 5years were 50%, 12%, and 9% respectively. The median survival time was 59weeks in limited disease and 44 weeks in extensive disease. 3) The median time to progression was 49weeks in responders. 4) Leukopenia(grade III, IV) was 7.8% and thrombocytopenia(grade III, 1V) was 2.0%. Anorexia, nausea, and alopecia was observed in most cases, but the degree was mild to moderate. 5) Toxicities to radiation therapy were radiation pneumonities in 50%, radiation esophagities in 28%. The delay of chemotherapy due to radiation toxicity occured in 72%, In the patients with limited disease receiving thoracic radiation therapy, relapse rates were 88.9%. The intrathoracic relapse occured in 35% and extrathoracic relapse occured in 65%. Conclusion - VPP regimens in small cell lung cancer were less toxic than other regimens, but it's efficacy was equiva1ent. Thereafter futher investigations are required for new chemotherapeutic agents, higher dose chmotherapy and autologous bone marrow transplantation and modifying drug to resistance to chemotherapy, Also, the investigations for methods which can increasing efficacy and decreasing toxicity of radiation therapy for limited disease are required.

인천지역 설사환자에서 분리한 Salmonella enterica serovar Enteritidis의 항생제 내성 및 다제내성 양상

황경화,오보영,김정희,김명희,제갈승,이은주,이은정,조남규,고종명,김용희,Hwang, Kyoung-Wha,Oh, Bo-Young,Kim, Jung-Hee,Kim, Myeong-Hee,JeGal, Seung,Lee, Eun-Ju,Lee, Eun-Jung,Cho, Nam-Kyu,Go, Jong-Myoung,Kim, Yong-Hee 한국미생물학회 2009 미생물학회지 Vol.45 No.2

본 연구에서는 2004년 1월부터 2008년 12월까지 인천지역 5개 종합 병 의원에 설사증상으로 입원 또는 내원한 환자의 대변에서 분리한 115주의 S. Enteritidis의 항생제 내성 및 다제내성 양상을 모니터링하였다. 그 결과, 가장 내성율이 높은 항생제는 nalidixic acid (54.8%)이었고, ampicillin (40.0%), ticarcillin (38.3%), chloramphenicol (20.9%), tetracycline (17.4%) 순이었다. 분리균의 91.3%는 1제 이상의 항생제에 내성이 있었다. 항생제 내성 유형을 살펴본 결과 nalidixic acid 단일 내성이 44.3%로 가장 높았으며, ampicillin-chloramphenicol-ticarcillin (11.3%) 및 ampicillin-ticarcillin (10.4%) 순이었다. 전체적으로 1제 내성이 51.9%, 2제 내성 18.2%, 3제 내성 14.5%, 4제 내성 5.8% 그리고 5제 이상 내성은 9.7%이었다. This study describes the antimicrobial susceptibility and multidrug resistance patterns in 115 isolates of Salmonella enterica serovar Enteritidis from diarrhea patients in Incheon from Jan. 2004 to Dec. 2008. Overall, the highest rate of resistance was found to the following antimicrobial agents: nalidixic acid 54.8%, ampicillin 40.0%, ticarcillin 38.3%, chloramphenicol 20.9%, and tetracycline 17.4%. 91% (104 isolates) were resistant to one or more antimicrobial agents tested. Among the described antimicrobial resistant patterns, the predominant patterns were only nalidixic acid (44.3%), ampicillin-chloramphenicol-ticarcillin (11.3%,) and ampicillin-ticarcillin (10.4%). Overall, the resistant rate to one drug was 51.9%, two drugs 18.2%, three drugs 14.5%, four drugs 5.8%, and five or more drugs 9.7%.

복숭아 품종 판별용 SCAR 마커를 활용한 국내 육성 품종 판별

한상은(Sang Eun Han),조강희(Kang-Hee Cho),남은영(Eun Young Nam),신일섭(Il-Sheob Shin),김정희(Chun Hee Kim),김현란(Hyun Ran Kim),김대현(Dae-Hyun Kim) 한국원예학회 2010 한국원예학회 학술발표요지 Vol.2010 No.10

Deep Learning기반의 Mass Spectrum 분석 기법

손종욱(Jong-Wuk Son),최은숙(Eun-Sook Choi),이성영(Sung Young Lee),김정희(Jung-Hee Kim),김은주(Eunjoo Kim),조국래(Kookrae Cho) 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.2

딥러닝 기법이 여러 연구 분야에서 많이 활용되고 있지만 mass 스펙트럼과 같은 고차원 데이터 분석에서는 활용도가 높지 않다. 본 논문에서는 mass 스펙트럼 과 같이 데이터의 차원수에 비해 샘플의 수가 적은 데이터에 대해서 딥러닝 기법을 적용하는 방법에 대해 기술한다. 이를 위해 TOF-SIMS 장비를 이용해서 186명의 CRC 환자 조직에서 558개의 mass 스펙트럼을 획득 하였으며 딥 러닝 기법을 적용하여 mass 스펙트럼을 이용해서 정상 조직과 대장암 조직간에 구분하는 모델을 구현하였다. Mass 스펙트럼 데이터를 딥러닝을 이용해서 분석하는 방식이 필요한 이유는 유전자데이터, 단백질 데이터를 조합하여 추가적인 분석이 가능하다는 장점이 있다.

콜린의 결핍과 서로 다른 지방이 2 - Acetylaminofluorene 을 투여한 쥐간의 Glutaghione 의존 효소에 미치는 영향

조은영,최혜미,김현아,변기원,송지현,김정희 ( Eun Young Cho,Hay Min Choi,Hyeona Kim,Ki Won Byun,Ji Hyun Song,Jun Ghee Kim ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.4

Weanling Sprague-Dawley male rats were fed the diet containing either 15% beef tallow or 15% corn oil with or without 0.075% choline chloride for 10 weeks. At 3rd and 5th week, animals were given injection of 2-acetylaminofluorene (AAF) at a dose of 50 ㎎/㎏ body weight twice a week (1st and 3rd day of the week). At 11th week, all animals were decapitated. Microsomal lipid peroxide values, microsomal enzyme activities of glucose-6-phosphatase (G6Pase), cytosolic enzyme activities of glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) were determined. Microsomal lipid peroxide values measured as malondialdehyde (MDA) were increased significantly in choline deficient corn oil group, but not significantly in any other groups. Microsomal G6Pase activities were decreased in corn oil groups compared with beef tallow groups, and choline deficient corn oil group had the lowest value. Cytosolic GSH-Px activities tended to be elevated by choline deficiency in both fat groups. Cytosolic GR activities tended to be decreased by choline deficiency in beef tallow group, but significantly increased in corn oil group. Activities of cytosolic GST were tended to be increased by choline deficiency in both fat groups. All glutathione-dependent enzyme activities were significantly higher in corn oil groups than in beef tallow groups and the highest in choline deficient corn oil group. These results suggest that polyunsaturated fatty acid (PUFA) diet and choline deficiency may increase hepatic microsomal lipid peroxidation. Although lipid peroxidation induces protective enzymes, destruction of membrane integrity is inevitable. When 2 factors were compared on lipid peroxidation, induction of protective enzymes, and destruction of membrane integrity, PUFA diet was more effective than choline deficient diet.