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국내 약용자원식물의 지방산 조성과 ${\gamma}-linolenic$ acid 탐색
김정봉,김용환,이철희,황영수,박노동,Kim, Jung-Bong,Kim, Young-Hwan,Lee, Cheol-Hee,Hwang, Young-Soo,Park, Ro-Dong 한국약용작물학회 1995 한국약용작물학회지 Vol.3 No.2
Fatty acid composition analysis was carried out from 60 species of Korean native medicinal plant to screen ${\gamma}-linolenic$ acid(GLA) resources. Oenothera odorata, SymPhytum officinal, and Lithospermum erythrorhizon contained ${\gamma}-linolenic$ acid with 9.5%, 3.5%, 7.2% of total fatty acid content, respectively. The vegetative organs usually contained higher level of ${\alpha}-linolenic$ acid than seeds. Some plants were found to be excellent source of unsaturated fatty acid. 우리나라에서 생산되고 있는 60여종의 약용식물의 지방산 조성을 조사하여 식물체의 부위 와 종류에 따른 지방산 조성의 특성을 관찰한 결과 아래와 같은 결과얻었다. 1. 달맞이꽃종자, 컴프리잎, 지치뿌리에서 각각 총지방산함량의 9.1, 3,5, 7,2%의 GLA가 검출되었으며 달맞이 꽃종자가 컴프리잎이나 지치뿌리보다 GLA의 절대량이 많았다. 2. 들깨종자, 참깨종자, 호박종자등의 지방산이 양(量)과 불포화도면에서 좋은 자원임이 확인되었다. 3. 식물체의 잎, 줄기, 뿌리에서의 지방산조성은 종자의 경우에 비하여 18 : 3의 분포 비율이 현저히 높게 나타났으며 18 : 0의 비율은 비교적 같게 나타났다.
청갓과 적갓에 함유된 Glucosinolates의 항암 활성 및 정량 분석
김활 ( Hwal Kim ),김준열 ( Jun Yeol Kim ),김효진 ( Hyo Jin Kim ),김도경 ( Do Kyung Kim ),조혜진 ( Hye Jin Jo ),한병수 ( Byoung Su Han ),김헌웅 ( Heon Woong Kim ),김정봉 ( Jung Bong Kim ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.3
The objective of this study was to compare the anticancer activity of glucosinolates against four different cancer cells; SNU-251, SNU-354, SNU-C4, MCF-7 and to determine the amounts of glucosinolates in mustard leaves. Green and red mustard leaves were cultivated on the field of Rural Development Administration from May to July, 2010. After the leaves were harvest and lypophilized, the fine powder was extracted with boiling 70% methanol(v/v) for the measurement of anticancer activity and then applied to the cancer cells obtained from Korean Cell Line Bank(KCLB). The anticancer activity of red leaf mustard was higher than that of green mustard leaf, particularly in SNU-251 for 24 hr and in SNU-C4 for 48 hr. In both green and red mustard leaf, sinigrin was the most abundant glucosinolte and was determined as 4.71 and 3.06 ㎎/g dry wt., respectively, but glucoiberin only presented 0.14 ㎎/g dry wt. with minor amounts.
연구보문 : 수질환경 ; 국산과 수입산 천일염의 지방산 분석
김수연 ( Su Yeon Kim ),김정봉 ( Jung Bong Kim ),김헌웅 ( Heon Woong Kim ),김세나 ( Se Na Kim ),김소영 ( So Young Kim ),조영숙 ( Young Sook Cho ),김재현 ( Jae Hyun Kim ),원항연 ( Hang Yun Weon ),함경식 ( Kyung Sik Ham ) 한국환경농학회 2011 한국환경농학회지 Vol.30 No.4
BACKGROUND: Inorganic component is made up largely of salt, because the criteria are difficult to split into domestic and imported ingredients, organic examined the fatty acid composition is to see a possible use as a marker using gas chromatography-mass spectrometry (GC-MS). The 14 domestic samples and 8 imported samples were collected from China, India and other countries were investigated on item of 37 fatty acids. METHODS AND RESULTS: The major components were six species, myristic acid (C14: 0), palmitic acid (C16: 0), palmitoleic acid (C16: 1), stearic acid (C18: 0), oleic acid (C18: 2, n9), linoleic acid (C18: 2, n6) among detected twenty fatty acids including 9 unknowns. The content of palmitic acid were highest as 25.2 to 50.8% of total fatty acids contents. Domestic has seen the most amount of salt in the Taepyeong salts 250.8㎍/100g, imports 135.2㎍/100g salt in Chinese Weifang salts showed the lowest content. CONCLUSION(s): The total fatty acid content has seen the most amount of salt in 352.3 ug/100g Christmas island salts showed the lowest content of 164.0㎍/100g, as a result it is difficult to distinguish the domestic salts and imported by the composition of fatty acid.
연구보문 : 식물환경 ; 곡류 도정에 따른 지방산 조성 변화 연구
조영숙 ( Young Sook Cho ),김유나 ( Yu Na Kim ),김수연 ( Su Yeon Kim ),김정봉 ( Jung Bong Kim ),김헌웅 ( Heon Woong Kim ),김세나 ( Se Na Kim ),김소영 ( So Young Kim ),박홍주 ( Hong Ju Park ),김재현 ( Jae Hyun Kim ) 한국환경농학회 2011 한국환경농학회지 Vol.30 No.4
BACKGROUND: Cereals, especially rice is the staple food of oriental nations and because it is very important for Korean food, to determine the extent of nutrient losses due to milling, we analyzed the fatty acid using by GC-FID(Gas Chromatography-Flame Ionization Detector). Experimented rice cereals were rice, glutinous rice, Heuinchalssalbori, Seodunchalbori, Saessalbori, Keunalbori No.1, barnyard millet produced in Korea. METHODS AND RESULTS: After milling, the contents of fatty acids in the rice, glutinous rice, Heuinchalssalbori, and Seodunchalbori, Keunalbori No.1 rather decreased, but in the Saessalbori, and barnyard mille increased. Particularly, fatty acid content of the rice decreased from 24.8㎎/g to 6.4㎎/g, glutinous rice decreased from 29.4 mg/g to 11.7㎎/g after milling. There were also significant changes in the compositions of fatty acid among samples. Stearic acid (C18:0) increased from 5% to 15%, but oleic acid (C18:1) and linoleic acid (C18:2) decreased in the rice after milling. CONCLUSION(s): In the brown rice, 11 different types of fatty acids were detected, and its highest content was found in grains. However, milled grain was observed only seven fatty acids in the case of rice. This result insisted that a portion of the lipid layer was significantly lost during the milling operation in rice.
김정봉 ( Jung Bong Kim ),김민희 ( Min Hee Kim ),이영이 ( Young Yi Lee ),이호선 ( Ho Sun Lee ),이석영 ( Sok Young Lee ),김헌웅 ( Heon Woong Kim ),추상미 ( Sang Mi Chu ),백형진 ( Hyung Jin Baek ),조영숙 ( Young Sook Cho ),김재현 ( J 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.3
A methodological study was conducted to test the potential of near-infrared reflectance spectroscopy (NIRS) for the estimation of fatty acid composition and content in sesame seeds. A set of 3237 sesame lines, provided from the Rural Development Administration, were scanned by NIRS for their fatty acids. Calibration equations for the content of fatty acids were developed for the 122 lines of them by GC-FID(gas chromatography-flame ionization detector). Main fatty acids were palmitic acid(C(16:0), 8.5%), stearic acid(C(18:0), 5.7%), oleic acid(C(18:1), 39.3%), linoleic acid(C(18:2), 43.4%), and linolenic acid(C(18:3), 0.4%); the lowest and highest level were IT028899-7.2% and IT169359-10.0% in palmitic acid and IT028897-4.5%, IT028910-7.0% in stearic acid, IT170020-32.2% and IT156371-46.5% in oleic acid, IT156348-36.5 and IT169926-49.6% in linoleic acid, and IT169316-0.2% and IT156367-0.6% in linolenic acid, respectively. Determination of the fatty acid composition of seeds could be carried out successfully by using the spectra patterns of NIRS for both oleic and linoleic acids. As a result, oleic acid and linoleic acid compositions(% of total fatty acid) ranged from 25.8% to 49.2% and from 21.7% to 52.2%, respectively, in sesame seeds. Correlation(r²) between the percentage of oleic acid and linoleic acid was calculated in (-0.23).
Gas Chromatography-Mass Spectroscopy를 이용한 γ-Linolenic Acid(GLA) 생성 균주탐색 및 확인
김정봉 ( Jung Bong Kim ),김경환 ( Kyung Hwan Kim ),홍승범 ( Seung Beom Hong ),박종석 ( Jong Sug Park ),이종렬 ( Jong Yeoul Lee ),김삼선 ( Sam Sun Kim ),배신철 ( Shin Chul Bae ),조강진 ( Kang Jin Cho ),이동진 ( Dong Jin Lee ) 한국균학회 2007 韓國菌學會誌 Vol.35 No.2
Delta 15 desaturase 유전자 억제에 의해 알파리놀렌산 함량이 낮은 들깨 육성
김경환(Kyung-Hwan Kim),이경렬(Kyeong-Ryeol Lee),김정봉(Jung-Bong Kim),이명희(Myoung Hee Lee),이은경(Eungyeong Lee),김년희(Nyunhee Kim),이홍석(Hongseok Lee),김송림(Song Lim Kim),백정호(JeongHo Baek),최인찬(Inchan Choi),지현소(Hyeonso 한국육종학회 2018 한국육종학회지 Vol.50 No.4
Perilla is an oilseed crop cultivated in Korea since ancient times. Due to the high α-linolenic acid content in perilla, perilla seed oil can easily become rancid. α-Linolenic acid is synthesized by two enzymes, endoplasmic reticulum-localized Δ15 desaturase (FAD3) and chloroplast-localized Δ15 desaturase (FAD7) in vivo. In order to lower the α-linolenic acid content of the seed oil without disturbing plant growth, we tried to suppress the expression of only the FAD3 gene using RNA interference, whilst maintaining the expression of the FAD7 gene. Seventeen transgenic plants with herbicide (Basta TM ) resistance were obtained by Agrobacterium-mediated transformation using hypocotyls of perilla plants. The transgenic plants were firstly confirmed by treatment with 0.3% (v/v) Basta TM herbicide, and the expression of FAD3 was measured by Northern blot analysis. The α-linolenic acid content was 10-20%, 30-40%, and 60% in two, seven, and three of the twelve T1 transgenic perilla plants which had enough seeds to be analyzed for fatty acid composition, respectively. Analysis of the fatty acid composition of T2 progeny seeds from T1 plants with the lowest α-linolenic acid content showed that the homozygous lines had 6-10% α-linolenic acid content and the heterozygous lines had 20-26% α-linolenic acid content. It is expected that the reduction in α-linolenic acid content in perilla seed oil will prevent rancidity and can be utilized for the production of high-value functional ingredients such as high γ-linolenic acid.