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치주질환자의 면역글로블린 이종형에 따른 제한절편장 다변화 양상에 대한 PCR 기법의 개발
최점일,김성조,김인후,Choi, Jeom-Il,Kim, Sung-Jo,Kim, In-Hoo 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.2
The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
박상언 ( Sang Un Park ),최은숙 ( Eun Sook Choi ),장연실 ( Yeon Sil Jang ),홍승희 ( Seung Hee Hong ),김인후 ( In Hoo Kim ),장동경 ( Dong Kyung Chang ) 대한소화기학회 2011 대한소화기학회지 Vol.57 No.3
Background/Aims: Tetraploid cells are frequently observed in the inflamed mucosal epithelial cells of the patients with Barrett`s esophagus or chronic ulcerative colitis. Polyploidy often occurs during cell fusion, abortive cell cycle, and endoreplication. Most tetraploid cells are engaged to apoptotic pathway, but some remaining stable tetraploid cells consequently cause aneuploidization and chromosomal instability. We investigated whether tetraploid cells could acquire survival advantage and hold a dominant position for natural selection. Methods: We established tetraploid cell line (HCT116GH) from parental diploid colorectal cancer cell line (HCT116) via PEG-mediated cell fusion and compared its cell viability, cell cycle response and apoptotic fractions responded to H2O2 with diploid HCT116 and p53 suppressed HCT116/H6 cell lines. Results: Using MTT assay, plating efficiency and clonogenicity, we evaluated the survival of each cell line. Tetraploid cell line HCT116GH demonstrated an 83 fold greater resistance to 100 μM H2O2 than the parental diploid HCT116, and 6 fold greater than even the p53 negative diploid HCT116/E6. Cellular sensitivity, G2/M arrests, and apoptotic proportion were observed less in response to H2O2 in HCT116GH compared with HCT116 and HCT116/E6. HCT116GH expressed lower level of p53 and p21 than diploid HCT116. Conclusions: Stable tetraploid cell lines showed enhanced viability in comparison to parental diploid cell lines. The enhanced viability observed in tetraploidization surpassed that from downregulation of p53. Frequent appearance of tetraploid cells in stressful condition can be caused by natural selection owing to their enhanced viability and may consequently contribute to cancer cell transformation.(Korean J Gastroenterol 2011;57:150-157)
3자 물류(Third Party Logistics: TPL)를 통한 물류 아웃소싱 혁신 사례 연구: 한솔 CSN을 중심으로
황선일 ( Sun Il Hwang ),허대식 ( Dae Sik Hur ),김태현 ( Tae Hyun Kim ),김인후 ( In Hoo Kim ) 한국로지스틱스학회 2009 로지스틱스연구 Vol.17 No.1
21세기는 아웃소싱의 시대라고 말해도 과언은 아니다. 기업경쟁의 세계화로 인해서 기업들은 아웃소싱을 통해서 고정자산에 대한 투자를 줄이고, 동시에 핵심역량의 강화에 나머지 자원을 집중적으로 투자하는 선택과 집중이 경쟁의 원칙이 되고 있다. 물류전문업체의 등장과 함께 많은 기업들이 자사물류에서 3자 물류로 전환하고 있지만, 3자 물류회사들에게 통합물류 서비스를 위탁하는 예는 많지 않은 것이 현실이다. 본 연구에서는 한국의 대표적 3자 물류회사인 한솔 CSN의 사례를 통해서, 화주기업의 통합물류 아웃소싱의 배경, 장애요인, 3자 물류회사의 역할에 대해서 심도 있는 분석을 하고, 시사점을 제시하고 있다. We live in the era of outsourcing. As the world market becomes more integrated and competition has intensified, more and more firms prefer to be `asset-light` while outsourcing non-core operations to outside specialists. In particular, logistics is the most often outsourced business processes. Yet, 3rd party logistics service firms have not been penetrated to the integrated logistics market, and many firms are still reluctant to increase their reliance on 3rd party logistics service providers. In this study, we reported a case study of Hansol CSN to shed a light on this issue, and suggested several managerial implications.
건조 혈액 여과지에서의 β-Globin DNA와 RNA의 추출 및 그 안전성에 관한 검토
김덕인,김인후,김정만,한진영,오성용,최용천 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3
연구배경: Guthrie spot는 1963년부터 신생아 유전 질환의 선별검사에 사용되기 시작한 이후로 최근에는 DNA와 RNA를 이용한 분석에도 응용이 시도되고 있다. 저자들은 일반 여과지에 채취된 건조 혈액으로부터 β-globin 유전자의 DNA와 RNA를 추출하는 데에 있어서 여과지의 전처리 조건이 미치는 영향과 특히 장기 보관에 따른 RNA의 안정성을 살펴보고자 하였다. 방법: 전처리를 하지 않은 경우, 5% HCI로 전처리를 한 경우, 고압 멸균을 한 경우, 그리고 5% HCI 전처리 후 고압 멸균을 한 경우의 네가지로 나누어서 정상인의 혈액을 떨어뜨린 후 상온에서 2주 동안 보관하였다가 각각 DNA와 RNA를 추출하여 PCR과 RT-PCR을 실시하였다. 그리고 2년간 보관하였던 전처리를 하지 않은 건조 혈액 여과지에서 RNA를 추출하여 RT-PCR을 시행하고 염기서열 분석을 하여 β-globin 유전자인지를 확인하였다. 결과: 여과지의 전처리 여부에 관계없이 DNA와 RNA는 PCR에 의해 기대된 크기의 증폭 산물을 나타내었고, 2년간 장기 보관하였던 여과지에서도 RNA가 안정하게 보존되어 있어서 RT-PCR 생성물을 염기서열 분석한 결과, β-globin 유전자의 일부임을 확인할 수 있었다. 결론: 건조 혈액 여과지를 이용한 β-globin 유전자의 DNA와 RNA 분석은 검체의 채취와 보관이 용이하고 안정성이 높아서 혈색소 이상증을 포함한 유전 질환의 대규모 연구나 선별 검사에 매우 유용할 것으로 사료된다. Background: Since newborn screening efforts were facilitated by the use of Guthrie spots in 1963, the development of a routine procedure for microextraction of DNA and recently even RNA from these specimens would allow direct screening at the molecular level. The purpose of the current investigation was to optimize pretreatment condition of filter paper for improvement of microextraction and stability of β-globin DNA and RNA from dried blood specimens on Whatman filter papers. Methods: Normal whole blood was spotted on filter papers pretreated by four different ways, respectively ; untreated, 5% HCI treated, autoclaved, and 5% HCI treated and autoclaved. The samples were then stored at room temperature for two weeks until processed. DNA and RNA were extracted from each filter paper and amplified by PCR(polymerase chain reaction) and RT(reverse transcriptase)-PCR. We also extracted RNA from untreated dried blood spot stored at room temperature for two years for cDNA synthesis and did nucleotide sequencing to identify that the PCR products were part of β-globin gene. Results: DNA and RNA were stable in filter papers, and could be microextracted for PCR and RT-PCR regardless of pretreatment conditions. It also has been demonstrated that RNA could be obtained from dried blood spots stored for two years in sufficient quality and quantity for amplification and sequencing. Conclusion: The prolonged stability of β-globin DNA and RNA in these specimens will be very helpful for conducting a mass screening and research in genetic diseases including hemoglobinopathies principally because of the ease of sample collection, storage, handling, and shipment.
부산지역 C형 간염 바이러스 genotype에 관한 역학적 연구
김인후,김정만,신해림,이명기,김준연,정갑열,김인식,서병성,신우원,양학도,허윤영,송주복 동아대학교 부설 산업의학연구소 1999 산업의학연구소 논총 Vol.- No.4
The authors investigated the distribution of Hepatitis C virus (HCV) genotype in blood donors with positive for anti-HCV (n=34), health check up examinees with positive for anti-HCV (n=29), and in patients with various chronic liver diseases positive for anti-HCV (n=63) in Pusan, Korea. HCV genotype was determined by using the molecular typing method through the reverse transcription - polymerase chain reaction (RT - PCR) with four type specific primers. Among 116 anti-HCV positive study subjects, 66.4% were positive HCV RNA by RT-PCR. The major HCV genotype was type Ⅱ (31.9%) and it was followed by type Ⅲ (27.6%). Two cases were type Ⅳ (1.7%). Double infection with two different HCV genotypes (mixed type) was found in three cases (2.6%). Three cases (2.6%) were not determined by the four type specific primers, it may have different subtype. Type Ⅱ was more prevalent than type Ⅲ in the blood donors and health check-up examinees, but the reverse was true in the chronic liver diseases patients including hepatocellular carcinoma patients, Type Ⅱ was more prevalent than type Ⅲ among the anti-HCV positive subjects with risk factors such as acupuncture history, surgical operation history, and transfusion history. In contrast type Ⅲ was more prevalent than type Ⅱ among the subjects without the above risk factors. It is supposed that the pathogenicity of different kind of HCV genotype might be different. The results of this study suggest that the type Ⅱ and type Ⅲ may be the major HCV genotype in Korea. The differences of HCV genotype distribution between the study groups support that the clinical significance according to the HCV genotype may be different.