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        Streptomyces peucetius subsp . caesius 돌연변이주에 의한 doxorubicin 생산의 최적배양조건

        김승욱,송수문,문순옥 ( Seung Wook Kim,Soo Moon Song,Soon Ok Moon ) 한국공업화학회 1997 공업화학 Vol.8 No.4

        본 연구에서는 Streptomyces peucetius subsp. caesius 돌연변이주에 의한 doxorubicin의 생산에 있어서 배양조건 및 배지의 성분을 확립하여 doxorubicin의 생산성을 높이는데 목적이 있다. Doxorubicin 생산을 위한 최적 배지조성은 4% maltose, 0.5% HEPES, 0.02% K₂HPO₄, 0.01% MgSO₄로 나타났고, 가장 적합한 종균 접종량과 시기는 10%(v/v), 72시간이었다. Doxorubicin생산에 적합한 소포제를 찾기위해 여러 종류의 소포제를 배지에 첨가한 결과 가장 적합한 소포제는 KG(10% K+10% G)이었으며 최적농도는 0.01%이었다. 교반식반응기에서 배양할 경우 적합한 통기량은 1.5v/v min으로 최대 29mg/l의 doxorubicin을 생산하였고, 1.0v/v min의 경우에도 플라스크 배양보다 15% 증가된 23mg/l의 doxorubicin을 생산하였다. The production of doxorubicin by a mutant of Streptomyces peucetius subsp. cazesius was studied. The optimal culture conditions, such as inoculum size and medium composition were established to improve the productivity of doxorubicin. The optimal medium composition was found to be 4% maltose, 0.5% HEPES, 0.02% K₂HPO₄, 0.01% MgSO₄. As an antiform agent, 0.01% KG(10% Adekanol+10% Silicone) was suitable one among various agents. Culture was carried out in 2.5 L jar-fermenter with different aeration rates of 1.5, 1.0, and 1.5 v/v min. The maximum production of doxorubicin(29 mg/l) was obtained at 1.5 v/v min of aeration rate, and even at 1.0 v/v min, the production of doxorubicin was increased up to 15% compared with that of shake-flask culture.

      • KCI등재

        U-Healthcare를 위한 Visualization Engine 개발

        김승욱,김민철,Kim, Seung-Wook,Kim, Min-Cheol 한국정보통신학회 2008 한국정보통신학회논문지 Vol.12 No.10

        최근 웰빙, 건강 등을 추구하는 보건의료분야에 대한 관심이 증가하면서, 언제, 어디서나 자신의 건강에 대한 상태를 모니터링 할 수 있도록 가능하게 하는 u-Healthcare 서비스가 주목받고 있다. 기존의 의료정보시스템은 측정값의 저장과 단순 디스플레이만이 지원되는 구조로 구성되어 있으나, 이러한 의료정보시스템에서 사용자가 편리하고 효율적인 상태확인이나 진단을 하기 위해서는 원하는 의료정보를 빠르고 정확하게 제공해주도록 원활한 상호 작용을 해야 할 필요성이 있다. 이와 관련하여 본 연구결과는 u-Healthcare의 다양한 서비스에서 보다 사용자 친화적(user-friendly)이면서도 더 나은 정보변별력을 제공하는 Visualization Engine에 대한 개발적 내용을 제시하였다. As the interest on healthcare area in pursuit of well-being, health etc is increasing, u-Healthcare service that can monitor the condition of oneself's health anytime & anywhere is getting an attention. The existing health information system is consist of structure supporting the storage of estimation value and just simple display. But this needs to get a harmonious interaction to provide the health information exactly and speedily for efficient condition confirmation and diagnosis in health information system, In relation to this, this study proposed the developmental contents about visualization engine providing the beuer information discrimination and more user-friendly in various service of u-Healthcare.

      • KCI등재

        다층 PVD 코팅을 이용한 SKD 61다이캐스팅 강의 표면 특성 비교 분석

        김승욱,Kim, Seung Wook 한국반도체디스플레이기술학회 2021 반도체디스플레이기술학회지 Vol.20 No.2

        The properties of materials which are widely used in industry fields like automobile, shipbuilding, casting, and electronics are strongly needed to have higher surface hardness, lower surface roughness, and higher compressive residual stress. As mentioned above, for the purpose of satisfying three factors, a variety of researches with respect to surface improvement have been actively studied and applied to every industry. SKD61 which is mostly used for die casting process of cold chamber method must meet a countless number of problems which are thermal, mechanical and chemical from highly specific working environment at high temperature over 600℃. Above all, in case of plunger sleeves used for die casting process, thermal fatigue has a bad effect on the surface of an inlet where molten metal is repeatedly injected. On account of it, plunger sleeves cause manufacturers to deteriorate quality of products. Therefore, in this paper, to improve the surface of an inlet of plunger sleeve, multilayer PVD coating using Ti, Cr and Mo is suggested. Furthermore, The surface characteristics such as surface roughness(Rsa, Rsq), surface hardness(HRB, HRC) and residual stress using XRD(X-ray diffractometer) of coated samples and specimens are studied and discussed.

      • KCI등재

        스핀코터 회전속도에 따른 탄탈륨 박막두께의 선형모델에 관한 연구

        김승욱,Kim, Seung Wook 한국반도체디스플레이기술학회 2020 반도체디스플레이기술학회지 Vol.19 No.1

        Recently, the decrease in thin film thickness has been actively studied by changing several physical elements such as the increase in revolution velocity of lower substrate equipped with AC or DC motor. In this paper, we propose a novel spin coater control system that changes AC or DC motor and common use software with limitation of velocity and position control into step motor and LABVIEW software based on GUI to control revolution velocity and position more precisely. By determining six input values of rotation velocity 1, 5, 10, 25, 50, 100 PPS, we fabricated six samples using coating target, TA(tantalum) on silicon substrate and measured their thin film thickness by SEM. Hence, this research can be applied to inferring thin film thickness of tantalum regarding any value of revolution velocity without additional experiments and for linear reference model via property analysis of thin film thickness using other thin-film materials.

      • SCOPUSKCI등재

        열분해-가스크로마토그래피/질량분석법에 의한 가황고무중의 유기첨가제의 직접분석

        김승욱,허귀석,이계호,Kim, Seung Wook,Heo, Gwi Suk,Lee, Gae Ho 대한화학회 1997 대한화학회지 Vol.41 No.10

        Analysis of additives in cured rubber is often a difficult task for analytical chemists because of a wide variety of complex components. Conventional analyses of additives and rubbers have been done in multistep, off-line processes with large sample size and extensive sample preparations. The coumarone-indene resin, resorcinol-formaldehyde resin, and prevulcanization inhibitor have been characterized by their pyrolysis pathways and mass spectra of characteristic pyrolyzates. Pyrolysis Gas Chromatography/Mass Spectrometry (Py-GC/MS) was used in the identification of additives without any sample pretreatment. This result shows that several organic additives in cured rubber can be directly analyzed. 고무가황체는 고분자인 천연고무 및 합성고무에 여러 가지 유기첨가제를 배합하여 만든다. 이와 같은 배합고무의 분석은 여러 가지 다른 성질을 갖는 다양한 첨가제들로 인하여 많은 전처리 과정을 거쳐야 하므로 그 분석이 용이하지 않다. 이를 극복하기 위하여 열분해 가스크로마토그래피질량분석법(Pyrolysis Gas Chromatography-Mass Spectrometry)을 이용하여 고무가황체 중의 유기 고무약품들의 직접분석을 시도하였다. 점착제인 쿠마론-인덴수지(coumarone-indene resin $C-90^{TM}$), 접착제인 알킬페놀 레조시놀 포름알데히드 수지, 가류지연제 등의 고무첨가제의 열분해과정 및 열분해체들의 질량스펙트럼들을 확인하였고 이를 통하여 복잡한 구성 성분으로 이루어진 가황고무 중에서 이들 배합제의 직접분석이 가능하였다.

      • KCI등재후보
      • KCI등재후보

        『銀行週報』와 上海 金融業의 公論 형성

        김승욱(Kim Seung-wook) 가천대학교 아시아문화연구소 2009 아시아문화연구 Vol.17 No.-

        The Bankers Weekly was a banking business magazine that was published from 1917 to 1950 in Shanghai. In this study, the early magazine issues from 1917 to 1925 were analyzed. In various aspects ranging from the participating personnel to the media characteristics and the article contents, the magazine displayed a clearly distinguished trait, which was very rare among business magazines at that time. In this regard, the magazine stimulates researchers' interest. This study focused on the magazine's role from the terms of institution and public opinion. At that time, the financial business in Shanghai did not have a unified institution. In such a situation, financial organizations and entities separately formed their own business and transaction regulations that were applied in their divided scope. Needless to say, this reflected the disunified aspect of modern and contemporary China and Shanghai. However, such disunification also indicated that experiments were actively under progress. In this regard, how a unified institution was built through the experiments of institution becomes an intriguing question. Hereby, this study aims to illuminate what role the magazine played in forming the public opinion that led to the institution reform at that time. The Bankers Weekly, from the beginning, set its direction to reform the institution and promote public debates for it. As the magazine began as a medium sharing knowledge of finance which took infa medium and academic study as its two axes, sharing knowledge was closely related to critical views about the existing financial institution. Besides, they intended to set up The Bankers Weely as an oganization that formed the public opinion based on such critical knowledge. The personnel participating in The Bankers Weekly had quite a solid sharing of unified goal. This shared goal clearly distinguished themselves from the existing financial circles, and the perception that businesses in the industry needed cooperation to overcome their feeble foundation. Moreover, their experience of cooperation and collaborative struggle due to the Order of Suspension of Specie Payment made their joint effort even stronger. It is rare that a group that publicizes a magazine is clearly described, but The Bankers Weekly draws much attention in that its staff clearly had capability and direction to form public opinion. In analyzing the contents of the articles in The Bankers Weekly, a case was found where it had actively played the media's role as a space for public opinion. In this magazine, introductions to financial institutions in the West and China, and reports on them were posted. While they were critically discussed, the subjects of reforms about clearinghouses and replacing liang with yuan emerged. Various elements chaotically coexisted in the Shanghai financial business by forming contradictory regulations and institutions. To combat this chaos, The Bankers Weekly, accumulated relevant knowledge, identified concrete reform issues through analyses, and led to a public opinion to finally draw out a stream toward a unified institution. In terms of this, it can't be denied that this magazine played a significant role in forming public opinion in the financial circles in Shanghai.

      • KCI등재
      • KCI등재

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