http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이계호,고정삼,이강흡,Lee, Ke-Ho,Koh, Jeong-Sam,Lee, Kang-Hup 한국응용생명화학회 1976 Applied Biological Chemistry (Appl Biol Chem) Vol.19 No.3
전보(前報)에서 검토한 효소생산 최적조건에서 생성하는 cellulase의 특성(特性)에 대한 실험(實驗)을 행(行)하여 다음의 결과(結果)를 얻었다. 1. 조정제시(粗精製時)에 $(NH_4)_2SO_4$가 0.6포화도일 때 그리고 ethanol 농도(濃度)가 $80{\sim}90%$일 때 회수성(回收性)이 가장 좋았다. 2. 두 균주가 생산한 cellulase의 효소작용 최적 조건은 다음과 같다. Asp. niger-SM6 T. viride-SM10 최적(最適) pH 3.5 4.0 pH 안정성(安定性) $3.0{\sim}6.0$ $3.0{\sim}6.0$ 최적온도(最適溫度) $60^{\circ}C$ $60^{\circ}C$ 열안정성(熱安定性) $60^{\circ}C$이하(以下) $50^{\circ}C$이하(以下) 최적작용시간(最適作用時間) 30분(分) 60분(分) 최적기질농도(最適基質濃度) 3% 3% 3. CMCase에 대한 Km치(値)는 Aspergillus niger-SM6이 0.8%, Trichoderma viride-SM10이 1.01%였다. 4. Aspergillus niger-SM6은 xylanase 및 pectinase도 높은 활성(活性)을 가지고 있었다. In order to investigate the properties of enzymes from two strains of mold, reported in the previous paper, (1) studies have been made concerning the characteristics of cellulase of Aspergillus niger-SM6 and Trichoderma viride-SM10, and summarized as follows. 1. In the semi-purification the recovery of ${\beta}-glucosidase$ was the highest when 80-90% ethanol was used and 0.8 saturation of $(NH_4)_2SO_4$. 2. The characteristics of the semi-purified enzyme were as follows. Aspergillus niger-SM6 Trichoderma viride-SM10 Optimum pH 3.5 4.0 pH stability 3.0-6.0 3.0-6.0 Optimum temperature $60^{\circ}C$ $60^{\circ}C$ Heat stability below $60^{\circ}C$ below $50^{\circ}C$ Optimum reaction time 30 min. 60 min. Optimum CMC concentration 3% 3% 3. The Km values of CMCase were 0.8% and 1.01 for Aspergillus niger-SM6 and Trichoderma viride-SM10, respectively. 4. In the strain of Aspergillus niger-SM6, there were high activity of xylanase and pectinase.
내열성세균에 관한 연구 2 (제 2 ) 기성세균의 내열성에 관하여
이계호,장건형 한국미생물학회 1966 미생물학회지 Vol.4 No.2
The purpose of this paper is to study on the thermal death time curve and F-values, and morphological and physiological characteristics observed for the identification. The three strains of thermal resistancing anaerobic bacteria isolated from unheated various cans and swelled cans and the different soils collected from the wide area in Korea. The results obtained in the light of the manual of Bergeg's for the identification of the anareobic bacteria have been shown that the three strains of anaerobic bacteria are pertained to Cl. sporogenes B-41 Cl. butyricum B-72 & Cl.. botulinum Type E B-163. The optimum temperature, pH and thermal resistance, thermal death point of the anaerobic have been measured.