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박남용,배성열,기혜영,정치영,오기석,이철호,Park Nam-Yong,Bae Seong-Yeol,Kee Hye-Young,Chung Chi-Young,Oh Ki-Seok,Lee Cheol-Ho 대한수의사회 1991 대한수의사회지 Vol.27 No.8
Pathological investigations including light and electron microscopy were carried out on the two dead of the four and five-month-old diseased Korean cattle calves in order to find out the nature of a domestically unknown disease prevailing at a cattle farm
박남용,정치영,이창영,기혜영,배성열,이봉주,하용공,윤석민,정병탁,김동성,Park, Nam-yong,Chung, Chi-young,Ri, Chang-yeong,Kee, Hye-young,Bae, Seong-yeol,Lee, Bong-ju,Ha, Yong-kong,Yoon, Seog-min,Jung, Byung-tack,Kim, Dong-sung 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.4
Encephalomyocarditis virus infection in pigs, characterized by severe reproductive failure in sows and sudden death in less than 7 day-old-piglets, a period of several months from October to December of 1989, in Korea was occurred. The most outstanding gross lesions at necropsy were found in the heart which were consisted of pale, yellow brown necrotic foci of varying size in myocardium. In some cases, pulmonary edema and liver congestion were observed. Histopathology of the heart revealed the interstitial myocarditis, endocarditis and epicarditis with lymphocytic, plasmacytic and macrophage cell infiltration and, in some cases, calcification of the necrotic myocardial muscle fibers. In some cases of brain, the perivascular cuffing and glial nodules were observed. In the liver and the lacrimal gland varying degrees of multifocal necrosis were seen. The virus was isolated from the heart and the brain in a stillborn piglet and a mummified fetus. This outbreak represents the first case of encephalomyocarditis virus infection of pigs in Korea.
국내에서 발생한 돼지 뇌심근염 바이러스 감염증의 병리학적 관찰
박남용,이창영,정치영,기혜영,배성열,Park, Nam-yong,Ri, Chang-yeong,Chung, Chi-young,Kee, Hye-young,Bae, Seong-yeol 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.1
Pathological studies by light and electron microscope were carried out on the twenty piglets naturally affected by encephalomyocarditis virus infection. Gross findings included pale or yellow, small necrotic foci on myocardium, together with pulmonary edema and liver congestion in some cases. On light microscopy, nonsuppurative interstitial endocarcitis, epicarditis and myocarditis, myocardial infarction, and dystrophic calcification or fibtosis were observed in heart. Perivascular cuffings, gliosis and nonsuppurative meningitis were appeared in brain. Focal or diffuse necrosis with mononuclear cell infiltration in lacrimal gland and multifocal necrosis in liver were observed in some cases. Congestion and edema of lung, hyperemia, hemorrhage and deletion of lymphocytes of lymph nodes and spleen were recognized. On electron microscopy, severe swelling and vacuolization of mitochondria and sarcoplasmic reticulum, large intracellular vacuolation and edema, separation and fragmentation of myofibrils were observed. Virus particles were seen in the sarcoplasm of degenerated cardiac muscle cell.
2000년-2009년 광주 광역시 지역의 급성설사환자의 분변에서 분리한 살모넬라 균주에서의 항생제 내성률 조사
김태순,김민지,김선희,서진종,기혜영,정재근,김은선,문용운,하동룡,김민경,임숙경,남향미,Kim, Tae Sun,Kim, Min Ji,Kim, Sun Hee,Seo, Jin-Jong,Kee, Hye Young,Chung, Jae Keun,Kim, Eun Sun,Moon, Yong Woon,Ha, Dong Ryong,Kim, Min Kyeong,Lim, Suk The Microbiological Society of Korea 2013 미생물학회지 Vol.49 No.2
지난 2000년부터 2009년까지 10년간 광주광역시 지역의 급성설사환자로부터 분리한 총 596개의 살모넬라균주에 대하여 총 16종의 항생제에 대한 감수성 검사를 실시한 결과, 내성빈도가 가장 높았던 약제는 ampicillin (43%), tetracycline (35.9%), nalidixic acid (31.5%), 그리고 chloramphenicol (26.2%) 순으로 나타났다. 혈청형 간에 항생제 내성률에 차이가 있었는데 S. Enteritidis와 S. Typhimurium이 각각 가장 높은 내성률을 보인 약제는 ampicillin (51.1%)과 tetracycline (77.9%)이었다. 총 89가지 내성패턴이 관찰되었으며, 26% (155/596)의 살모넬라 분리주는 검사한 16가지 약제에 모두 감수성을 보였다. 약 21% (127/596) 및 15% (87/596)의 분리주는 각각 한 개 및 두 개의 약제에 내성을 나타냈다. 그 외의 살모넬라 분리주(227/596, 38%)는 세가지 이상의 약제에 내성을 나타냈다(다제내성균). 다제내성균이 가장 높은 비율로 나타난 혈청형은 S. Paratyphi B (76.5%), S. Typhimurium (58.2%), 그리고 S. Enteritidis (40.2%)의 순이었다. 가장 흔한 다제내성패턴은 ampicillin-chloramphenicol-nalidixic acid-ticarcillin (36/227, 15.8%)이었으며 이러한 패턴을 보였던 다제내성균의 대부분(35/36, 97.2%)이 S. Enteritidis이었다. Antibiotic susceptibility was examined for 596 Salmonella isolates from patients with acute gastroenteritis during 2000-2009 in Gwangju area in South Korea. Of 16 antibiotics tested, ampicillin resistance (43%) was the most commonly observed resistance among the 596 Salmonella sp. isolates, followed by tetracycline (35.9%), nalidixic acid (31.5%), and chloramphenicol (26.2%). Antibiotic resistance varied among serotypes: The highest resistance of S. Enteritidis and S. Typhimurium was to ampicillin (51.1%) and tetracycline (77.9%), respectively. A total of 89 resistance patterns were observed, and 26% (155/596) of Salmonella isolates were susceptible to all antibiotics tested in this study. About 21% (127/596) and 15% (87/596) of the isolates were resistant to one and two antibiotics, respectively. The rest of Salmonella isolates (227/596, 38%) were resistant to three or more antibiotic agents. The highest multi-drug resistance (MDR) was observed in serotype S. Paratyphi B (76.5%), followed by S. Typhimurium (58.2%), and S. Enteritidis (40.2%). The most common resistance pattern of MDR isolates was ampicillin-chloramphenicol-nalidixic acid-ticarcillin (36/227, 15.8%), most of which (35/36, 97.2%) were S. Enteritidis.
하용공,윤석민,정병탁,박남용,이봉주,정치영,기혜영,배성열,Ha, Yong-kong,Yoon, Seok-min,Jung, Byung-tack,Park, Nam-yong,Lee, Bong-ju,Chung, Chi-young,Kee, Hye-young,Bae, Seong-yeol 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.4
Encephalomyocarditis(EMC) virus was isolated from the mummified and stillborn pigs at a swine farm in Chonnam Province, experienced with EMC infection over the period Oct.~Dec. of 1989. In addition some cultural, serological properties of the isolates and experimental infections in the piglets were studied. The results obtained were as follows; 1. Two EMC virus strains with HA titers and CPE similar to EMC-ATCC were established in a baby hamster kidney (BHK)-21 cell line by inoculating homogenates of brain and heart of the 19 mummified or stillborn pigs and designated $K_3$ and $K_{11}$. 2. At the second BHK-21 cell line passage of the initial isolates CPE appeared after incubation for 16~18 hours, while at the fourth and fifth passage the highest titer of HA was recorded, titer of HA using rat and guinea pig erythrocytes. 3. One pig inoculated with the isolate $K_3$ showed dyspnea as clinical signs and died at the 10 days after inoculation at necropsy white necrotic foci were observed from the dead animal heart. 4. Although all the rest surviving pigs showed increases in antibody titer and body temperature of $40^{\circ}C$ above for the initial 2~4 days followed by the return to normal, there were no gross lesions when the animals were sacrificed at the 2 weeks after inoculation.
임소영(Soyoung Lim),문수진(Sujin Mun),이다빈(Davin Lee),양용식(Yongshik Yang),김여경(Yeo Kyung Kim),김혜진(Hye Jin Kim),정혜진(Hye Jin Jeong),김진영(Jinyeong Kim),박수정(Su Jung Park),기혜영(Hye Young Kee),이향희(Hyang Hee Lee),박정희(J 한국농약과학회 2024 농약과학회지 Vol.28 No.1
본 연구는 광주지역 유통 농산물을 대상으로 시험법 및 중점검사항목 개정 전, 후의 잔류농약 모니터링 결과를 비교하고 부적합 농산물로 인한 잠재적인 건강 위해성을 평가하기 위하여 수행되었다. 2020년부터 2022년까지 광주지역에 유통된 농산물 총 12,817건을 대상으로 식품의약품안전처의 다종농약다성분 분석법에 따라 전처리한 후 LC-MS/MS와 GC-MS/MS를 이용하여 311종(2020년 1월 ~ 2021년 9월)과 340종(2021년 10월 ~ 2022년 12월)의 농약을 분석하였다. 잔류농약 검출률 및 최대 잔류허용기준(MRL)을 초과한 부적합률은 2020년 45.8% (1.0%), 2021년 47.1% (1.4%), 2022년 54.2% (2.8%)로 매년 증가하였다. 시험법 및 검사항목 개정 후의 잔류농약 검출률 및 부적합률은 45.7% (1.2%)에서 53.9% (2.6%)로 증가하였다. 부적합 농산물은 주로 엽채류와 엽경채류였으며, 3년 동안 부적합 빈도가 가장 높은 품목은 쑥갓이었다. 최근 3년간 다항목 동시 검출률은 지속적으로 증가한 것으로 확인되었다(51.2%, 51.6%, 64.0%). 연도별 잔류허용기준을 가장 많이 초과한 농약성분은 diazinon (10건), fluopyram (12건), dinotefuran (14건)이었다. 부적합 농산물에 대한 위해성 평가 결과를 근거로 판단하였을때 모든 부적합 농산물에서 위해지수가 100% 이하로 나타나 안전한 수준으로 확인되었다. The purpose of this study was to compare the results of analysis of residual pesticides according to changes in the test method and principal items for residual pesticides and to evaluate potential risks to human health from contaminated agricultural products in Gwangju. GC-MS/MS and LC-MS/MS were performed on 12,817 samples collected from 2020 to 2022 and analysis for 311 (from Jan 2020 to Sept 2021) and 340 (from Oct 2021 to Dec 2022) pesticides was performed using multi-residue methods included in the Korean Food Code. The detection rates and violation rates that exceeded the maximum residue limit (MRL) showed a steady increase to 45.8% (1.0%) in 2020, 47.1% (1.4%) in 2021, and 54.2% (2.8%) in 2022. Following the revision of the test method and principal items, the detection rates and violation rates increased from 45.7% (1.2%) to 53.9% (2.6%). The contaminated agricultural products mainly included leafy vegetables and stalk and stem vegetables, and crown daisy was the most contaminated agricultural product over a period of three years. A continual increase in the rates of multi-residue pesticides detected has been observed over the past three years (51.2%, 51.6%, and 64.0%). Diazinon (10), Fluopyram (12), and Dinotefuran (14) were the pesticides detected most frequently by year. The results of risk assessment showed that the hazard index was below 100% for the contaminated agricultural products, which was confirmed as a safe level.
광주지역 HIV/AIDS, 매독 혈청 양성률 및 임질 양성률 분포조사(2002-2006)
기혜영,최병선,서진종,김선희,김민지,김은선,박종태,정재근 대한감염학회 2009 감염과 화학요법 Vol.41 No.4
Background : The epidemic of human immunodeficiency virus (HIV) or sexually transmitted infections (STIs) is an important public health issue in the worldwide. Therefore, we investigated the trends of the seroprevalence of HIV and, syphilis and the gonorrhea positive rates in Gwangju from 2002 to 2006. Materials and Methods : The results of laboratory tests for HIV, syphilis and gonorrhea were collected from five public health centers and 19 hospitals in Gwangju. The number of tested patients from 2002 to 2006 was 348,202 cases for HIV, 80,115 cases for syphilis and, 103,548 cases for gonorrhea. Results : HIV/AIDS infections have rapidly increased at public health centers and hospitals. Especially, most of newly HIV-infected people were patients on ambulatory care/hospitalization, and they were also found via preoperation tests and according to the clinical symptoms. The gonorrhea positive rates have gradually decreased by year while the syphilis infection rates have slightly increased from 2002 to 2006. Conclusions : We have to determine the reasons why HIV and syphilis infections, but not gonorrhea, have increases simultaneously by the year. These results should be helpful to create a more effective STD prevention policy and the treatment guidelines for the people in Gwangju in the near future.