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석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율
임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2
This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.
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유장렬,유문식,남부현 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2
Peritonsillar abscess occurs when the bacterial infection of the palatine tonsil spreads to the potential peritonsillar space deep behind the tonsil. Recently, the incidence of peritonsillar abscess was decreased due to the advent of antibiotics but not uncommon complication of acute tonsillitis. A clinical observation was performed on 42 cases of peritonsillar abscess who admitted to the department of otolarngology in Chungnam National University from Jan. 1986 to Sep. 1990. The obtained results were as follows : 1. The most frequently involved age group was third decade. 2. Most prevalent season was spring(33%). 3. Side of abscess was left in 23 cases and right in 19 cases. 4. Among 42 cases, 19 cases were performed of aerobic culture and 14 cases were performed of anaerobic culture. In aerobic culture, 6 strains of α-hemolytic Streptococci, 4 strains of β-hemolytic Streptococci and 1 strain of Enterobacter cloaca were found. In anaerobic culture, 1 strain of Peptostreptococci and 1 strain of anaerobic Streptococci were found. 5. 39 patients were treated with incision and drainage, 16 patients of these were treated with interval tonsillectomy. 3 patients were treated with antibiotics only.
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차장현,이정완,유능수,남윤수 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B
In this paper, remote monitoring system for wind turbine is developed. The developed system consists of data acquisition for wind sensor, and monitoring for site environment. In order to accomplish effective monitoring, the system uses Datasocket, SMB, FTP, Web Server, and G Web Server. Two computer system - one is data acquisition computer using Windows-XP and the other is monitoring computer using UNIX - constraint the distribute system with individual tasks. By using this system, one can perform various monitoring and control tasks in Wind-Turbine application, efficiently.
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차장현,이정완,유능수,남윤수 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.A
In this paper, remote monitoring system for wind turbine is developed. The developed system consists of data acquisition for wind sensor, and monitoring for site environment. In order to accomplish effective monitoring, the system uses Datasocket, SMB, FTP, Web Server, and G Web Server. Two computer system - one is data acquisition computer using Windows-XP and the other is monitoring computer using UNIX - constraint the distribute system with individual tasks. By using this system, one can perform various monitoring and control tasks in Wind-Turbine application, efficiently.
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H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향
정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4
Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.
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정해룡, 유장환, 윤성현, 권수정, 우선희 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.2
Adzuki bean is sensitive to waterlogging stress. The overall study on the waterlogging stress is limited comparing to the study on the drought and any environmental stress. Therefore, the present study was conducted to investigate the changes in growth characteristics and the expression patterns of proteins at the 5-leaf stage periods of adzuki bean. The domestic cultivar, Arari (Miryang No. 8) was used to test the waterlogging stress. In the waterlogging treatment for 3 days, the plant height showed slightly decrease in the treatment at 3 days of waterlogging, but root fresh weight showed significantly changes at 3 days of the waterlogging treatment. Chlorophyll contents showed also significantly different at 3 days of waterlogging treatment compared to control the plants. The waterlogging stress gradually influenced the growth differences between the control and the treatment respectively. More than 350 pro- tein spots were identified on 2-D gels using an image analysis. Moreover, a total of 28 proteins were analyzed using LTQ-FT-ICR MS. Among these 28 proteins, a total of 18 proteins were up-regulated, and 10 proteins were down-regulated under waterlogging treatment. According to biological process, the most of the proteins were found to be involved in carbohydrate metabolism. Regarding the subcellular local- ization, most of the proteins were localized into chloroplasts in 5-leaf stage.
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모 제약회사 근로자들의 직업성 천식 유병률과 약제 분말 노출수준
오성수,최용휴,김은아,이영목,장재길,유장진,박승현,김태균,정수영,김규상,강성규 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2
목적: 제약회사에서 직업상 천식의 유병 규모를 파악하고 작업공정에 대한 작업환경 측정을 통해 제약회사의 약제 분말에 대한 노출수준을 평가하고자 하였다. 방법: 제약회사에서 약제 분말에 노출되는 근로자를 대상으로 천식을 선별하기 위하여 설문조사 및 진찰, 혈액검사, 폐기능 검사 그리고 피부단자시험을 실시하였고, 각 부서별 공정에 따라서 총분진과 항생제 또는 효소제 분말에 대한 작업환경 측정을 실시하였다. 직업상 천식이 의심되는 근로자에 대하여 기관지과민성에 대한 검사(메타콜린을 이용한 비특이 기관지유발시험)와 약제 분말 기관지유발시험을 실시하여 확진하였다. 결과: 설문조사 및 진찰에서 작업 관련 천식 증상이 있는 근로자는 11명(34.4%)이었고, 약제 분말을 이용한 피부단자시험에서 1+ 이상 반응을 보인 근로자 10명(31.2%)이었다. 비특이 기관지유발시험 결과 8명(25.0%)이 양성(PC20 ≤16 mg/ml)이었다. 약제 분말 기관지유발시험을 통해 직업상 천식으로 확진된 근로자는 5명(15.6%)이었다. 작업환경 측정에서 원료를 충진하거나 투입하는 작업 그리고 정립작업에서 약제 분말에 대한 노출이 많았다. 결론: 제약회사에서 직업상 천식은 주로 제조공정 중 약제 분말에 노출된 근로자에서 발생하여, 증상 및 직업성 천식의 유병률이 높았다. 따라서 원료를 충진 또는 투입하는 작업과 정립작업 등 제조과적에서 약제 분말 노출을 줄이기 위한 세심한 주의가 필요하여, 약제 분말을 취급하는 근로자에 대해서는 천식 증상 조사뿐 아니라 정기적으로 피부단자시험, 비특이 기관지유발시험 등을 실시하여야 할 것이다. Objectives: After the investigation of one worker with occupational asthma, we surveyed the preva- lence of occupational asthma and the exposure level of pharmaceutical dust of 32 workers in a pharmaceutical company. Methods: Thirty-two of the 90 employees participated in the survey which consisted of questionnaire, blood sampling, spirometry and skin prick tests with 8 common allergens as well as 9 antibiotics and 2 enzymes. Various indices of the working environment were also measured. Subjects who had a symptom suggestive of work-related asthma or positive skin prick test were further investigated by PC20 methacholine. Nine subjects who had a PC20 result of 16 mg/ml or less (n=7) or had work-related symptoms and positive skin prick test (n=2) were referred to undergo a specific bronchial provocation test to phar- maceutical dust in an academic allergic disease center. Results: Eleven of 32 workers (34.4%) had a work-related symptom suggestive of occupational asthma. Ten (31.2%) showed positive skin prick test. 8 (25.0%) had a PC20 result of 16 mg/ml or less (indicative of significant bronchial hyperresponsiveness), and 5 (15.6%) had a positive result on the specific bronchial provocation test. Exposure levels of stuffing, input of raw materials and screening process were relatively high. Conclusions: This survey showed that pharmaceutical workers have an increased risk of occupational asthma. Although pharmaceutical factories maintain a relatively good working environment, careful control of respiratory tract exposures, especially during stuffing, input or raw materials and screening process, is important to prevent occupational asthma. Pharmaceutical workers need to undergo regular skin prick and methacholine bronchial provocation tests, as well as asthmatic symptom survey, to ensure the early detection and prevention of occupational asthma.
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Hyun-Jung Ji,Kyu-Hyun Park,Tae-Soo Kim,Sun-Young Rha,Nae-Choon Yoo,Jun-Myung Kim,Jun-Suk Kim,Jae-Kyoung Roh,Woo-Ick Jang,Hyun-Cheol Chung 대한암학회 2002 Cancer Research and Treatment Vol.34 No.3
Purpose: Activation of telomerase is proposed to bean essential step in cancer cell immortalization andcancer progression. 3’-azido-2’,3’-dideoxythymidine (AZT),a reverse transcriptase inhibitor, was reported to beincorporated in telomeric sequences of immortalizedcells in culture and to suppress the activity of telomeraseand the cell proliferation. In this study, after induction ofcancer cell senescence with long-term treatment of AZT,we investigated the dynamics of telomerase subunits(hTERT, hTR, TEP), transcription factors (c-Myc, Mad1),telomerase activity, and finally, telomere length in ahuman breast cancer cell line.Materials and Methods: Human breast cancer cell(MDA-MB-231) was treated with AZT. Senescence wasmeasured by senescence-associated β-gal staining andapoptosis was counted by dTd enzyme assay. Telomeraseactivity (by TRAP assay), expression of telomerasesubunit genes (by RT-PCR and real-time PCR) andtelomere length (by Southern blot analysis) were measuredafter the AZT treatment.Results: We found evidences of senescence, apoptosisand growth delay after AZT treatment. In addition, AZTtreatedcancer cells showed inhibition of telomeraseactivity and shortening of telomere length in a dose- andduration-dependent way. Among the telomerase subunits,hTERT and c-Myc were the first factors to change afterAZT treatment, subsequently, followed by the changes ofhTR, Mad1 and TEP.Conclusion: The suppression of hTERT and c-Myc byAZT treatment was the initial genetic phenomenon, subsequentlyfollowed by the changes of hTR, Mad1 and TEP.(Cancer Res Treat. 2002;34:223-233)
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