A Method of Pseudo 3D Video Reconstruction Based on 2D Video Sequences

Yong Yin,Lin Qi,Sen Qi 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.9

A method of pseudo 3D video reconstruction based on 2D video sequences is proposed, which transforms the conventional 2D video into their stereo version using original image and depth-map image. The red-component map is shifted left to obtain the left-view according to the parallax information. Because the left image has holes, the depth-map preprocessing and novel hole-filling simplified algorithm are used to fill the holes effectively. The experimental results show that the proposed algorithm generates 3D visual view with the same image quality as well as faster rendering speed compared with the reference hole-filling algorithm. The method adapts to the stereo pair synthesis for 3D.

Cluster Analyses of Tropical Cyclones with Genesis in the South China Sea Based on K‑Means Method

Yafeng Yin,Yangyang Yong,Shandong Qi,Kai Yang,Yizhao Lan 한국기상학회 2023 Asia-Pacific Journal of Atmospheric Sciences Vol.59 No.4

Tropical cyclone (TC) with genesis in the South China Sea (SCS) has been a major concern because of their high landfallfrequency and associated serious hazards to the surrounding coastal areas. The classification of TCs from records of historicaltracks is an important way to obtain their characteristics and to help predict their future behavior. According to the generationlocation, intensity, direction, and track length of TC, TCs with genesis in the SCS from 1950 to 2020 are classified into fourclusters by the K-means clustering method, including northwestward track cluster A, westward track cluster C and two longnortheastward track clusters B and D. The landfall probability, peak season, climate trend, lifespan, maximum wind speed,and power dissipation index show a significant distinction for each cluster. All clusters had a landfall probability exceeding50%, with the highest probability in cluster A (90.44%), followed by cluster C, cluster B, and cluster D with the lowestprobability (54.55%). The clustering results indicate that tracks of TCs are strongly affected by the distribution pattern of theWestern Pacific Subtropical High. When the WPSH moves southward, the southwesterly anomalies provide a significantlyfavorable steering flow for TC northeastward. Conversely, the WPSH located northward in July-September, the strongsoutheasterly anomaly favoring the northwestward movement of TC. From October to November, the WPSH shrinking insize gives way to the prevailing anomalous easterlies that steer the TCs westward. Further concerning the influence of TCsin the different clusters by the WPSH movement will be helpful for prediction in terms of the occurrence, track and landfallprobability of TCs in the SCS.

Polycystic Ovarian Syndrome: Dysregulation at the Reproductive-Metabolic Interphase

Benjamin K Tsang,Qi Wang,Ji Young Kim,Kai Xue,Jia-Yin Liu,Jae Yong Han 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s

Polycystic ovarian syndrome (PCOS) is a heterogeneous syndrome associated with follicle growth arrest, dysregulated sex hormone profile, hyperthecosis and insulin resistance. Chemerin, a novel adipokine, is associated with obesity and metabolic syndrome. Although obese women and in PCOS subjects have elevated plasma chemerin levels, whether and how chemerin is involved in the regulation of follicular growth/steroidogenesis and pathogenesis of PCOS is unknown. Our objective is to better understand the complex regulatory mechanisms involved in the control of these processes and gain insights in their dysregulation in the pathogenesis of PCOS. We hypothesize that: (a) hyperandrogenism induces small and medium antral follicle growth arrest and ovarian structural changes, resulting from granulosa cell and oocyte apoptosis and theca cell survival, and (b) chemerin regulates follicular growth and steroidogenesis and contributes to the pathogenesis of PCOS. Using immature rats (day 13~15 for follicle culture and day 21~24 for granulosa cells culture) and a chronically androgenized rat model [dihydrotestosterone (DHT); 83 μg daily, day 21~105] which recapitulates the reproductive and metabolic phenotypes of human PCOS, we have examined the granulosa cell expression patterns of chemerin and its receptor CMKLR1 and their steroidogenic and follicle growth capability. DHT treatment resulted in decreased follicle numbers in preantral to preovulatory stages and absence of corpus luteum, but increased numbers of condensed atypical follicles. Atypical follicles, constituted predominantly of theca cells, exhibited high expression of calpain and down-regulation of the cytoskeletal protein substrates vimentin, fodrin and β-tubulin. Granulosa cell aromatase expression was significantly down-regulated, a response accompanied by increased activated caspase-3 content and DNA fragmentation. While PTEN levels were considerably higher in granulosa cells in the PCOS rats than controls, phospho-Akt (Ser473) content was lower. In addition, DHT also activated granulosa cell caspase-3, decreased XIAP, PARP and phospho-Akt contents and induced apoptosis in vitro, responses that could be attenuated by forced expression of XIAP. These findings are consistent with our hypothesis that dysregulated follicular growth in PCOS is associated with changes in follicular growth dynamics and follicle cell fate, a consequence of dysregulated interactions of pro-survival (p-Akt, XIAP, PARP) and proapoptotic (calpain, PTEN, caspase-3) modulators in a cell-specific manner. Chemerin and CMKLR1 were expressed in granulosa cells and negatively regulated by gonadotropin in vivo and in vitro. Serum and ovarian chemerin levels in DHT-treated rats were elevated, and associated with arrested early antral follicular growth, remodeling of the follicle wall and decreased expression of p450 side-chain cleavage enzyme (p450- scc), aromatase and hydroxysteroid dehydrogenases. Recombinant chemerin inhibited FSH - induced estradiol secretion in granulosa cells from DHT-treated rats in vitro. Chemerin also suppressed basal and FSH- and GDF9-induced follicle growth and estradiol/ progesterone production in preantral follicle cultures. Moreover, chemerin suppressed FSH-induced p450scc/aromatase expression and progesterone/estradiol secretion in immature rat granulosa cells in vitro. These studies demonstrate that chemerin is a novel negative regulator in FSH-induced follicular growth and steroidogenesis and support the notion that the dysregulation of chemerin expression and function contributes to pathogenesis of PCOS. Our observations also suggest that this chronically androgenized rat model may be useful not only for studies on the long term effects of androgen on folliculogenesis, but also on the pathophysiology of PCOS. * This work was supported by grants from the Canadian Institutes of Health Research (CIHR; MOP-119381) and the World Class University (WCU) program through the Ministry of Education, Science and Technology funded by the National Research Foundation of Korea (R31-10056).

Polycystic Ovarian Syndrome: Dysregulation at the Reproductive-Metabolic Interphase

Benjamin K Tsang,Qi Wang,Ji Young Kim,Kai Xue,Jia‐Yin Liu,Jae Yong Han 한국동물번식학회 2012 Reproductive & developmental biology Vol.36 No.2

Inter-domain Alliance Authentication Protocol Based on Blind Signature

Zhang Jie,Zhang Qi-kun,Gan Yong,Yin Yifeng,Tan Yu-an 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.12

In large distributed networks, many computers must be mutual coordination to complete some works under the certain conditions, these computers may come from different domains. For ensuring secure cross domains to access resources among these computers in different domains, this paper proposes a multi-domain union authentication protocol. The protocol adopts blind signature to achieve mutual authentication among entities in different domains. This scheme overcomes the complexity of certificate transmission and the network bottlenecks in the scheme of PKI-based. It can trace the entity identity and supports two-way entities anonymous authentication, which avoid the authority counterfeiting its member to access other domain’s resources. Analysis shows that its advantages on security and communication-consumption.

Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq

( Gui-hua Lu ),( Yin-ling Zhu ),( Ling-ru Kong ),( Jing Cheng ),( Cheng-yi Tang ),( Xiao-mei Hua ),( Fan-fan Meng ),( Yan-jun Pang ),( Rong-wu Yang ),( Jin-liang Qi ),( Yong-hua Yang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3

The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.

Isolation of Probiotic Piliated Lactobacillus rhamnosus Strains from Human Fecal Microbiota Using SpaA Antiserum-Based Colony Immunoblotting

( Zhen-quan Yang ),( Yu Xue ),( Sheng-qi Rao ),( Mi Zhang ),( Lu Gao ),( Yong-qi Yin ),( Da-wei Chen ),( Xiao-hui Zhou ),( Xin-an Jiao ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.11

Piliated Lactobacillus rhamnosus (pLR) strains possess higher adherent capacity than non-piliated strains. The objective of this study was to isolate and characterize probiotic pLR strains in human fecal samples. To this end, mouse polyclonal antiserum (anti-SpaA) against the recombinant pilus protein (SpaA) of L. rhamnosus strain GG (LGG) was prepared and tested for its reactivity and specificity. With the anti-SpaA, a method combining the de Man, Rogosa, and Sharpe (MRS) agar plating separation and colony immunoblotting (CIB) was developed to isolate pLR from 124 human fecal samples. The genetic and phenotypic characteristics of the resultant pLR isolates were compared by randomly amplified polymorphic DNA (RAPD) fingerprinting, and examination of adhesion to Caco-2 cells, hydrophobicity, autoaggregation, and in vitro gastrointestinal tolerance. Anti-SpaA specifically reacted with three pLR strains of 25 test strains, as assessed by western blotting, immunofluorescence flow cytometry, and immunoelectron microscopy (IEM) assays. The optimized MRS agar separation plus anti- SpaA-based CIB procedure could quantitatively detect 2.5 × 10³ CFU/ml of pLR colonies spiked in 10⁶ CFU/ml of background bacteria. Eight pLR strains were identified in 124 human fecal samples, and were confirmed by 16S RNA gene sequencing and IEM identification. RAPD fingerprinting of the pLR strains revealed seven different patterns, of which only two isolates from infants showed the same RAPD profiles with LGG. Strain PLR06 was obtained with high adhesion and autoaggregation activities, hydrophobicity, and gastrointestinal tolerance. Anti-SpaA-based CIB is a rapid and inexpensive method for the preliminary screening of novel adherent L. rhamnosus strains for commercial purposes.

Direct ethanol production from dextran industrial waste water by Zymomonas mobilis

Ming-xiong He,Han Qin,Xiao-bo Yin,Zhi-yong Ruan,Fu-rong Tan,Bo Wu,Zong-xia Shui,Li-chun Dai,Qi-chun Hu 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.11

The direct production of ethanol from dextran industrial waste water was investigated by using Zymomonasmobilis via batch and semi-continuous fermentation mode. In batch fermentation, pretreated waste water (unsterilizedand sterilized), pH value (3.8 and 6.0), and Mg2+(with and without) was compared with OD600, sugar and ethanol con-centration. After 24 h fermentation, sugar in the dextran waste water was almost exhausted, and the amount of ethanolaccumulated reached 24.33-29.92 g/l, which is nearly 99% of the theoretical yield of ethanol. Kinetic parameters ofZ. mobilis in batch fermentation were also investigated. The raw dextran waste water was also used in semi-continuousfermentation. After 48 h fermentation, the production of ethanol was 28.65 g/l. These results indicated that dextranwaste water may be used as a candidate substrate and Z. mobilis could convert the raw material into ethanol directly.

Effect of nutritional factors on the accretion of secondary metabolites in Malaysian ginseng adventitious root cultures

Cui Xi-Hua,Hosakatte Niranjana Murthy,Zhang Ji-De,Song Hang-Lin,Jiang Yin-Ji,Qi Wen-Wen,Li Yong Yi,백기엽,박소영 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.3

In this study, we aimed to verify the effect of nutritional factors on the accretion of secondary metabolites in the adventitious root (AR) cultures of Malaysian ginseng (Eurycoma longifolia Jack) grown in small-scale bioreactors. AR were induced from leaf explants and cultured in different types of media including Murashige and Skoog (MS) medium, Driver Kuniyuki Walnut (DKW) medium, Gamborg’s B5 medium, Woody Plant Medium (WPM), and ¾ MS medium. Among these media, the MS and Gamborg’s B5 media induced lateral root development from initial inoculum, which accounted for the increase in AR biomass accretion. By contrast, the DKW and WPM media did not induce lateral root formation from the cultured explants. The ¾ MS medium was optimal for the growth of AR and accretion of secondary metabolites, after 7 weeks of culture, the biomass of AR increased by 8.6-fold in ¾ MS medium, and the total phenolic and flavonoid contents reached 5.23 and 2 mg g−1 of tissue dry weight, respectively. Analysis of mineral elements in the spent medium revealed that ¾ MS medium was most suitable for nutrient supply to developing AR. LC–MS analysis showed the accretion of eurycomanone, a therapeutically useful metabolite, in the AR of Malaysian ginseng.