Probabilistic Constrained Approach for Distributed Robust Beamforming Design in Cognitive Two-way Relay Networks

( Xueyan Chen ),( Li Guo ),( Chao Dong ),( Jiaru Lin ),( Xingwang Li ),( Charles C. Cavalcante ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.1

In this paper, we propose the distributed robust beamforming design scheme in cognitive two-way amplify-and-forward (AF) relay networks with imperfect channel state information (CSI). Assuming the CSI errors follow a complex Gaussian distribution, the objective of this paper is to design the robust beamformer which minimizes the total transmit power of the collaborative relays. This design will guarantee the outage probability of signal-to- interference-plus-noise ratio (SINR) beyond a target level at each secondary user (SU), and satisfies the outage probability of interference generated on the primary user (PU) above the predetermined maximum tolerable interference power. Due to the multiple CSI uncertainties in the two-way transmission, the probabilistic constrained optimization problem is intractable and difficult to obtain a closed-form solution. To deal with this, we reformulate the problem to the standard form through a series of matrix transformations. We then accomplish the problem by using the probabilistic approach based on two sorts of Bernstein-type inequalities and the worst-case approach based on S-Procedure. The simulation results indicate that the robust beamforming designs based on the probabilistic method and the worst-case method are both robust to the CSI errors. Meanwhile, the probabilistic method can provide higher feasibility rate and consumes less power.

Lattice Boltzmann simulation of the spreading behavior of a droplet impacting on inclined solid wall

Wanyu Chen,Fan Yang,Yonghua Yan,Xueyan Guo,Ren Dai,Xiaoshu Cai 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.6

The spreading behavior of a 3D droplet impacting on inclined solid wall is presented by using a lattice Boltzmann method (LBM) with single component multiphase pseudopotential model. The time evolution of spreading factor, dimensionless spreading width, dimensionless height, dimensionless bottom area and adhesion force are obtained. Our numerical results show that when increasing the contact angle or inclination angle, the dimensionless height and the dimensionless bottom area increases but the dimensionless spreading length and spreading width decreases. In addition, the progresses of circumferential spreading in starting-up and spreading stages and the aggregation at front edge in retracting stage would be more obvious by decreasing the viscosity or increasing the Bond number.

De novo design of a novel AIE fluorescent probe tailored to autophagy visualization via pH manipulation

Huang Xueyan,Chen Fei,Ma Yeshuo,Zheng Fan,Fang Yanpeng,Feng Bin,Huang Shuai,Zeng Hongliang,Zeng Wenbin 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Macroautophagy is an essential cellular self-protection mechanism, and defective autophagy has been considered to contribute to a variety of diseases. During the process, cytoplasmic components are transported via autophagosomes to acidic lysosomes for metabolism and recycling, which represents application niches for lysosome-targeted fluorescent probes. Additionally, in view of the complexity of the autophagy pathway, it entails more stringent requirements for probes suitable for monitoring autophagy. Meanwhile, aggregation-induced emission (AIE) fluorescent probes have been impressively demonstrated in the biomedical field, which bring fascinating possibilities to the autophagy visualization.We reported a generalizable de novo design of a novel pH-sensitive AIE probe ASMP-AP tailored to lysosome targeting for the interpretation of autophagy. Firstly, the theoretical calculation was carried out followed by the investigation of optical properties. Then, the performance of ASMP-AP in visualizing autophagy was corroborated by starvation or drugs treatments. Furthermore, the capability of ASMP-AP to monitor autophagy was demonstrated in ex vivo liver tissue and zebrafish in vivo.ASMP-AP displays a large stokes shift, great cell permeability and good biocompatibility. More importantly, ASMP-AP enables a good linear response to pH, which derives from the fact that its aggregation state can be manipulated by the acidity. It was successfully applied for imaging autophagy in living cells and was proved capable of monitoring mitophagy. Moreover, this novel molecular tool was validated by ex vivo visualization of activated autophagy in drug-induced liver injury model. Interestingly, it provided a meaningful pharmacological insight that the melanin inhibitor 1-phenyl-2-thiourea (PTU)-induced autophagy was clearly presented in wild-type zebrafish.ASMP-AP offers a simple yet effective tool for studying lysosome and autophagy. This is the first instance to visualize autophagy in zebrafish using a small-molecule probe with AIE characters, accurate lysosome targeting and simultaneous pH sensitivity. Ultimately, this novel fluorescent system has great potential for in vivo translation to fuel autophagy research.

An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides

( Hanyu Zhu ),( Xueyan Sun ),( Dongmei Liu ),( Liesheng Zheng ),( Liguo Chen ),( Aimin Ma ) 한국균학회 2017 Mycobiology Vol.45 No.4

An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus―Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 μg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.

Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line

Xusheng Dong,Chen Liu,Jialin Miao,Xueyan Lin,Yun Wang,Zhong-Hua Wang,Qiuling Hou 한국축산학회 2022 한국축산학회지 Vol.64 No.5

5-Hydroxytryptamine (5-HT), a monoamine, as a local regulator in the mammary gland is achemical signal produced by the mammary epithelium cell. In cows, studies have shown that5-HT is associated with epithelial cell apoptosis during the degenerative phase of the mammarygland. However, studies in other tissues have shown that 5-HT can effectively promotecell viability. Whether 5-HT could have an effect on mammary cell viability in dairy cows isstill unknown. The purpose of this study was to determine: (1) effect of 5-HT on the viability ofbovine mammary epithelial cells and its related signaling pathways, (2) interaction betweenprolactin (PRL) and 5-HT on the cell viability. The bovine mammary alveolar cell-T (MAC-T)were cultured with different concentrations of 5-HT for 12, 24, 48 or 72 hours, and then wereassayed using cell counting kit-8, polymerase chain reaction (PCR) and immunobloting. Theresults suggested that 20 μM 5-HT treatment for 12 or 24 h promote cell viability, which wasmainly induced by the activation of 5-HT receptor (5-HTR) 1B and 4, because the increasecaused by 5-HT vanished when 5-HTR 1B and 4 was blocked by SB224289 and SB204070. And protein expression of mammalian target of rapamycin (mTOR), eukaryotic translationelongation factor 2 (eEF2), janus kinase 2 (JAK2) and signal transducer and activator of transcription5 (STAT5) were decreased after blocking 5-HT 1B and 4 receptors. When MAC-Tcells were treated with 5-HT and PRL simultaneously for 24 h, both the cell viability and thelevel of mTOR protein were significantly higher than that cultured with 5-HT or PRL alone. Inconclusion, our study suggested that 5-HT promotes the viability of MAC-T cells by 5-HTR1B and/or 4. Furthermore, there is a reciprocal relationship between PRL and 5-HT.

A Rare Case of Extensive Erythrophagocytosis by Pathological Erythroblasts in a Patient With Myelodysplastic Syndrome

Qiang Xing,Li Jia,Chen Siyu,Gou Yang,Wang Ping,Chen Xueyan 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.3

An Enzymolysis-Assisted Agrobacterium tumefaciens-Mediated Transformation Method for the Yeast-Like Cells of Tremella fuciformis

( Yuanyuan Wang ),( Danyun Xu ),( Xueyan Sun ),( Lisheng Zheng ),( Liguo Chen ),( Aimin Ma ) 한국균학회 2019 Mycobiology Vol.47 No.1

Agrobacterium tumefaciens-mediated transformation (ATMT), as a simple and versatile method, achieves successful transformation in the yeast-like cells (YLCs) of Tremella fuciformis with lower efficiency. Establishment of a more efficient transformation system of YLCs is important for functional genomics research and biotechnological application. In this study, an enzymolysis-assisted ATMT method was developed. The degradation degree of YLCs depends on the concentration and digestion time of Lywallzyme. Lower concentration (_0.1%) of Lywallzyme was capable of formation of limited wounds on the surface of YLCs and has less influence on their growth. In addition, there is no significant difference of YLCs growth among groups treated with 0.1% Lywallzyme for different time. The binary vector pGEH under the control of T. fuciformis glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter was utilized to transform the enzymolytic wounded YLCs with different concentrations and digestion time. The results of PCR, Southern blot, quantitative real-time PCR (qRT-PCR) and fluorescence microscopy revealed that the T-DNA was integrated into the YLCs genome, suggesting an efficient enzymolysis-assisted ATMT method of YLCs was established. The highest transformation frequency reached 1200 transformants per 10⁶ YLCs by 0.05% (w/v) Lywallzyme digestion for 15 min, and the transformants were genetically stable. Compared with the mechanical wounding methods, enzymolytic wounding is thought to be a tender, safer and more effective method.

A Rapid and Effective Colony PCR Procedure for Screening Transformants in Several Common Mushrooms

( Yuanyuan Wang ),( Danyun Xu ),( Dongmei Liu ),( Xueyan Sun ),( Yue Chen ),( Lisheng Zheng ),( Liguo Chen ),( Aimin Ma ) 한국균학회 2019 Mycobiology Vol.47 No.3

In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant’s tissue (1-10 lg) to 20 ll 0.25% Lywallzyme solution, and vortexing for 10 s followed by incubation at 34 ℃ for 15 min. Finally, 2 ll of the suspension was used as templates to perform PCR and single target bands were successfully amplified from respective transformants of Tremella fuciformis, Pleurotus ostreatus, and Pleurotus tuber-regium. This procedure could be widely employed for screening transformants in mushroom transformation experiments.

Joint Space-time Coding and Power Domain Non-orthogonal Multiple Access for Future Wireless System

( Jin Xu ),( Hanqing Ding ),( Zeqi Yu ),( Zhe Zhang ),( Weihua Liu ),( Xueyan Chen ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.1

According to information theory, non-orthogonal transmission can achieve the multiple-user channel capacity with an onion-peeling like successive interference cancellation (SIC) based detection followed by a capacity approaching channel code. However, in multiple antenna system, due to the unideal characteristic of the SIC detector, the residual interference propagated to the next detection stage will significantly degrade the detection performance of spatial data layers. To overcome this problem, we proposed a modified power-domain non-orthogonal multiple access (P-NOMA) scheme joint designed with space-time coding for multiple input multiple output (MIMO) NOMA system. First, with proper power allocation for each user, inter-user signals can be separated from each other for NOMA detection. Second, a well-designed quasi-orthogonal space-time block code (QO-STBC) was employed to facilitate the SIC-based MIMO detection of spatial data layers within each user. Last, we proposed an optimization algorithm to assign channel coding rates to balance the bit error rate (BER) performance of those spatial data layers for each user. Link-level performance simulation results demonstrate that the proposed time-space-power domain joint transmission scheme performs better than the traditional P-NOMA scheme. Furthermore, the proposed algorithm is of low complexity and easy to implement.

Ratiometric and discriminative visualization of autophagic processes with a novel dual-responded lysosome-specific fluorescent probe

Fan Zheng,Yeshuo Ma,Jipeng Ding,Shuai Huang,Shengwang Zhang,Xueyan Huang,Bin Feng,Hongliang Zeng,Fei Chen,Wenbin Zeng 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Autophagy is a critical self-eating pathway involved in numerous physiological and pathological processes. Lysosomal degradation of dysfunctional organelles and invading microorganisms is central to the autophagy mechanism and essential for combating disease-related conditions. Therefore, monitoring fluctuations in the lysosomal microenvironment is vital for tracking the dynamic process of autophagy. Although much effort has been put into designing probes for measuring lysosomal viscosity or pH separately, there is a need to validate the concurrent imaging of the two elements to enhance the understanding of the dynamic progression of autophagy. Methods Probe HFI was synthesized in three steps and was developed to visualize changes in viscosity and pH within lysosomes for real-time autophagy tracking. Then, the spectrometric determination was carried out. Next, the probe was applied to image autophagy in cells under nutrient-deprivation or external stress. Additionally, the performance of HFI to monitor autophagy was employed to evaluate acetaminophen-induced liver injury. Results We constructed a ratiometric dual-responsive probe, HFI, with a large Stokes shift over 200 nm, dualwavelength emission, and small background interference. The ratiometric fluorescent signal (R = I610/I460) of HFI had an excellent correlation with both viscosity and pH. More importantly, high viscosity and low pH had a synergistic promotion effect on the emission intensity of HFI, which enabled it to specially lit lysosomes without disturbing the inherent microenvironment. We then successfully used HFI to monitor intracellular autophagy induced by starvation or drugs in real-time. Interestingly, HFI also enabled us to visualize the occurrence of autophagy in the liver tissue of a DILI model, as well as the reversible effect of hepatoprotective drugs on this event. Conclusions In this study, we developed the first ratiometric dual-responsive fluorescent probe, HFI, for real-time revealing autophagic details. It could image lysosomes with minimal perturbation to their inherent pH, allowing us to track changes in lysosomal viscosity and pH in living cells. Ultimately, HFI has great potential to serve as a useful indicator for autophagic changes in viscosity and pH in complex biological samples and can also be used to assess drug safety.