Response of Saccharomyces cerevisiae to Ethanol Stress Involves Actions of Protein Asrlp

( Jun Mei Ding ),( Xiao Wei Huang ),( Na Zhao ),( Feng Gao ),( Qian Lu ),( Ke Qin Zhang ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.12

During the fermentation process of Saccharomyces cerevisiae, yeast cells must rapidly respond to a wide variety of external stresses in order to survive the constantly changing environment, including ethanol stress. The accumulation of ethanol can severely inhibit cell growth activity and productivity. Thus, the response to changing ethanol concentrations is one of the most important stress reactions in S. cerevisiae and worthy of thorough investigation. Therefore, this study examined the relationship between ethanol tolerance in S. cerevisiae and a unique protein called alcohol sensitive RING/PHD finger 1 protein (Asr1p). A real-time PCR showed that upon exposure to 8% ethanol, the expression of Asr1 was continuously enhanced, reaching a peak 2 h after stimulation. This result was confirmed by monitoring the fluorescence levels using a strain with a green fluorescent protein tagged to the C-terminal of Asr1p. The fluorescent microscopy also revealed a change in the subcellular localization before and after stimulation. Furthermore, the disruption of the Asr1 gene resulted in hypersensitivity on the medium containing ethanol, when compared with the wild-type strain. Thus, when taken together, the present results suggest that Asr1 is involved in the response to ethanol stress in the yeast S. cerevisiae.

Clinicopathologic and Prognostic Significance of the Zinc Finger of the Cerebellum Family in Invasive Breast Cancer

Wei Han,Cong Zhang,Xiao-jiao Gao,Hua-bing Wang,Fang Chen,Fang Cao,Yong-wei Hu,Jun Ma,Xing Gu,Hou-zhong Ding 한국유방암학회 2018 Journal of breast cancer Vol.21 No.1

Purpose: Five members of the zinc finger of the cerebellum (ZIC) family—ZIC1, ZIC2, ZIC3, ZIC4, and ZIC5—have been shown to be involved in various carcinomas. Here, we aimed to explore the clinicopathologic and prognostic roles of ZIC family members in invasive breast cancer patients using immunohistochemical analysis, western blotting analysis, and real-time quantitative polymerase chain reaction (RT-qPCR). Methods: A total of 241 female invasive breast cancer patients who underwent radical mastectomy between 2009 and 2011 were enrolled. ZIC proteins in 241 pairs of breast tumors and corresponding normal tissues were investigated using immunohistochemistry and the clinicopathologic roles of proteins were analyzed using Pearson’s chi-square test. Kaplan-Meier curves and Cox regression analysis were also used to analyze the prognostic value of the ZIC proteins. In addition, 12 pairs of fresh-frozen breast tumors and matched normal tissues were used in the western blotting analysis and RT-qPCR. Results: Only ZIC1 expression in normal tissues was obviously higher than that in tumors (p<0.001). On multivariate analysis, ZIC1 expression (in overall survival analysis: hazard ratio [HR], 0.405, 95% confidence interval [CI], 0.233–0.702, p=0.001; in disease-free survival analysis: HR, 0.395, 95% CI, 0.234–0.669, p=0.001) was identified as a prognostic indicator of invasive breast cancer. Conclusion: ZIC1, but not the other proteins, was obviously decreased in breast tumors and associated with clinicopathologic factors. Thus, ZIC1 might be a novel indicator to predict the overall and disease-free survival of invasive breast cancer patients.

Optimizing Instruction Prefetching to Improve Worst-Case Performance for Real-Time Applications

Ding, Yiqiang,Yan, Jun,Zhang, Wei Korean Institute of Information Scientists and Eng 2009 Journal of Computing Science and Engineering Vol.3 No.1

While the average-case performance is important for general-purpose applications, worst-case performance is crucial for real-time systems to ensure schedulability and reliability. Recent work has shown that simple prefetching techniques such as the Next-N-Line prefetching can benefit both average-case and worst-case performance; however, the improvement on the worstcase execution time (WCET) is rather limited and inefficient. This paper presents two instruction prefetching approaches that are specially designed to enhance the worst-case performance, including the loop-based prefetching and WCET-oriented prefetching. Our experiments indicate that both instruction prefetching techniques can achieve better worst-case execution cycles than the Next-N-Line prefetching while having various impacts on the average-case performance.

Hepatic Angiomyolipoma: Contrast Patterns with SonoVue-enhanced Real-time Gray-scale Ultrasonography

Wei, Rui-Xue,Wang, Wen-Ping,Ding, Hong,Huang, Bei-Jian,Li, Chao-Lun,Fan, Pei-Li,Hou, Jun,He, Nian-An Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

This study was conducted to retrospectively evaluate the pattern of contrast enhancement with SonoVue on gray-scale ultrasonography of hepatic angiomyolipoma (HAML). Imaging features of 33 pathologically proven HAML lesions in 33 patients who underwent baseline ultrasound and contrast-enhanced ultrasonography (CEUS) were assessed retrospectively. All lesions were enhanced in the arterial phase and showed whole-tumor filling in. Thirty-two of 33 (97%) lesions showed early positive enhancement in the arterial phase. Twenty-three of these exhibited isoechoic or hyperechoic features in the portal phase. HAML demonstrate characteristic manifestations with SonoVue-enhanced real-time gray-scale ultrasonography.

New power equalization modulation strategy for CHB seven‑level inverters

Jun Gu,Run-Zhe Cai,Xiang-Yu Zhou,Chao Ding,Wei-Jian Wang 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.7

For cascaded H-bridge (CHB) multilevel converters, the traditional in-phase disposition (IPD) modulation strategy has excellent harmonic elimination characteristics, but it leads to a single state of the switching tubes and unbalanced output power of each unit. In this paper, a new power equalization modulation strategy and its optimization are proposed, which solves the problem of power equalization, while taking into account the harmonic elimination performance of the output voltage and power equalization time. The new power equalization modulation strategy makes the carrier rotate in different carrier layers in a new interval, and the voltage of each unit is readjusted using the redundant switching states. Thus, the output power equalization can be achieved in a shorter time. While maintaining the output characteristics of the existing modulation strategy, the new modulation strategy reduces the power equalization time and preserves the harmonic elimination characteristics of IPD. In addition, the optimized modulation strategy reduces the number of carriers, lowering the difficulty of digital control. Finally, simulation and experimental results are used to verify the correctness and feasibility of the new power equalization modulation strategy and its optimization.

Improving the electrical performance of HfInZnO-TFTs by introducing a thin ITO interlayer

Jun Li,Xing-Wei Ding,Jianhua Zhang,Wen-Qing Zhu,Xue-Yin Jiang,Zhi-Lin Zhang 한국물리학회 2014 Current Applied Physics Vol.14 No.8

We have fabricated hafniumeindiumezinc-oxide (HfInZnO) thin film transistors (TFT) with indiumetinoxide (ITO) interlayer. Compared with conventional HfInZnO-TFT, the electrical performance and bias stability of HfInZnO-TFTs with ITO interlayer are improved. HfInZnO-TFT with 4-nm-thick ITO interlayer shows a high mobility of 7.2 cm2/V s, a low threshold voltage of 0.13 V and a better bias stability. The performance enhancement is attributed to a decrease in interface trap state and an increase in carrier concentration. It suggests that introducing ITO interlayer at the ALD Al2O3/HfInZnO interface is an effective way to improve the electrical performance and bias stability.

Genetic Diversity of a Chinese Native Chicken Breed, Bian Chicken, Based on Twenty-nine Microsatellite Markers

Ding, Fu-Xiang,Zhang, Gen-Xi,Wang, Jin-Yu,Li, Yuan,Zhang, Li-Jun,Wei, Yue,Wang, Hui-Hua,Zhang, Li,Hou, Qi-Rui Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

The level of genetic differentiation and genetic structure in a Chinese native chicken breed, Bian chicken, and two controlled chicken populations (Jinghai chicken and Youxi chicken in China) were analysed based on 29 microsatellite markers. A total of 166 distinct alleles were observed across the 3 breeds, and 32 of these alleles (19.3%) were unique to only 1 breed. Bian chicken carried the largest number of private alleles at 15 (46.9%), followed by the Jinghai chicken with 12 private alleles (37.5%). The average polymorphism information content (0.5168) and the average expected heterozygote frequency (0.5750) of the Bian chicken were the highest, and those of the Jinghai chicken were 0.4915 and 0.5505, respectively, which were the lowest. Among 29 microsatellite loci, there were 15 highly informative loci in Bian chicken, and the other 14 were reasonably informative loci. The highly informative loci in Jinghai chicken and Youxi chicken were 17 and 14 respectively. Significant deviations from the Hardy-Weinberg equilibrium were observed at several locus-breed combinations, showing a deficit of heterozygotes in many cases. As a whole, genetic differentiation among the breeds estimated by the fixation index (Fst) were at 6.7% (p<0.001). The heterozygote deficit within population (Fis) was 22.2% (p<0.001), with the highest (0.249) in Bian chicken and lowest (0.159) in Youxi chicken. These results serve as an initial step in the plan for genetic characterization and conservation of the Chinese chicken genetic resource of Bian, as well as Jinghai and Youxi chickens.

Improved Global Maximum Power Point Tracking Method Based on Voltage Interval for PV Array under Partially Shaded Conditions

Ding, Kun,Wang, Xiang,Zhai, Quan-Xin,Xu, Jun-Wei,Zhang, Jing-Wei,Liu, Hai-Hao The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.4

The power-voltage (P-V) curve of photovoltaic (PV) arrays connected in parallel to bypass diodes would have several local maximum power points (LMPP) under partial shading conditions (PSC). Conventional maximum power point tracking (MPPT) methods fail to search for the global maximum power point (MPP) because the searched peak point may remain at the LMPP on the P-V curve under PSC. This study proposes an improved MPPT algorithm to ensure that PV arrays operate at global maximum power point (GMPP) under PSC. The proposed algorithm is based on a critical study and a series of observations of PV characteristics under PSC. Results show the regularity of voltage interval between LMPPs. The algorithm has the advantages of rapidly reaching GMPP, maintaining stability, and recovering GMPP quickly when the operating condition changes. Simulation and experimental results demonstrate the feasibility of the proposed algorithm.

A long-term tunnel settlement prediction model based on BO-GPBE with SHM data

Yang Ding,Yu-Jun Wei,Pei-Sen Xi,Peng-Peng Ang,Zhen Han 국제구조공학회 2024 Smart Structures and Systems, An International Jou Vol.33 No.1

The new metro crossing the existing metro will cause the settlement or floating of the existing structures, which will have safety problems for the operation of the existing metro and the construction of the new metro. Therefore, it is necessary to monitor and predict the settlement of the existing metro caused by the construction of the new metro in real time. Considering the complexity and uncertainty of metro settlement, a Gaussian Prior Bayesian Emulator (GPBE) probability prediction model based on Bayesian optimization (BO) is proposed, that is, BO-GPBE. Firstly, the settlement monitoring data are analyzed to get the influence of the new metro on the settlement of the existing metro. Then, five different acquisition functions, that is, expected improvement (EI), expected improvement per second (EIPS), expected improvement per second plus (EIPSP), lower confidence bound (LCB), probability of improvement (PI) are selected to construct BO model, and then BO-GPBE model is established. Finally, three years settlement monitoring data were collected by structural health monitoring (SHM) system installed on Nanjing Metro Line 10 are employed to demonstrate the effectiveness of BO-GPBE for forecasting the settlement.

Evaluation of Renal Oxygenation Level Changes after Water Loading Using Susceptibility-Weighted Imaging and T2* Mapping

Jiule Ding,Wei Xing,Dongmei Wu,Jie Chen,Liang Pan,Jun Sun,Shijun Xing,Yongming Dai 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.4

To assess the feasibility of susceptibility-weighted imaging (SWI) while monitoring changes in renal oxygenation level after water loading. Thirty-two volunteers (age, 28.0 ± 2.2 years) were enrolled in this study. SWI and multi-echo gradient echo sequence-based T2* mapping were used to cover the kidney before and after water loading. Cortical and medullary parameters were measured using small regions of interest, and their relative changes due to water loading were calculated based on baseline and post-water loading data. An intraclass correlation coefficient analysis was used to assess inter-observer reliability of each parameter. A receiver operating characteristic curve analysis was conducted to compare the performance of the two methods for detecting renal oxygenation changes due to water loading. Both medullary phase and medullary T2* values increased after water loading (p < 0.001), although poor correlations were found between the phase changes and the T2* changes (p > 0.05). Interobserver reliability was excellent for the T2* values, good for SWI cortical phase values, and moderate for the SWI medullary phase values. The area under receiver operating characteristic curve of the SWI medullary phase values was 0.85 and was not different from the medullary T2* value (0.84). Susceptibility-weighted imaging enabled monitoring changes in the oxygenation level in the medulla after water loading, and may allow comparable feasibility to detect renal oxygenation level changes due to water loading compared with that of T2* mapping.