Risk Factors for Early Recurrence of HBV-related Hepatocellular Carcinoma Meeting Milan Criteria after Curative Resection

Zhu, Wen-Jiang,Huang, Chu-Ying,Li, Chuan,Peng, Wei,Wen, Tian-Fu,Yan, Lv-Nan,Li, Bo,Wang, Wen-Tao,Xu, Ming-Qing,Yang, Jia-Yin,Jiang, Li Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: The prognosis of patients with hepatocellular carcinoma (HCC) after curative resection varies greatly. Few studies had investigated the risk factors for early recurrence (recurrence-free time ${\leq}$ 1 year) of hepatitis B virus (HBV)-related HCCs meeting Milan criteria. Methods: A retrospective analysis was performed on the 224 patients with HCC meeting Milan criteria who underwent curative liver resection in our center between February 2007 and March 2012. The overall survival (OS) rate, recurrence-free survival (RFS) rate and risk factors for early recurrence were analyzed. Results: After a median follow-up of 33.3 months, HCC reoccurred in 105 of 224 patients and 32 died during the period. The 1-, 3- and 5-year OS rates were 97.3%, 81.6% and 75.6% respectively, and the 1-, 3- and 5-year RFS rates were 73.2%, 53.7% and 41.6%. Cox regression showed alpha-fetoprotein (AFP) > 800 ng/ml (HR 2.538, 95% CI 1.464-4.401, P=0.001), multiple tumors (HR 2.286, 95% CI 1.123-4.246, P=0.009) and microvascular invasion (HR 2.518, 95% CI 1.475-4.298, P=0.001) to be associated with early recurrence (recurrence-free time ${\leq}$ 1-year) of HCC meeting Milan criteria. Conclusions: AFP > 800 ng/ml, multiple tumors and microvascular invasion are independent risk factors affecting early postoperative recurrence of HCC. In addition resection appears capable of replacing liver transplantation in some situations with safety and a better outcome.

CFD based simulations of flutter characteristics of ideal thin plates with and without central slot

Zhu, Zhi-Wen,Chen, Zheng-Qing,Gu, Ming Techno-Press 2009 Wind and Structures, An International Journal (WAS Vol.12 No.1

In this paper, the airflow around an ideal thin plate (hereafter referred to as ITP) with various ratios of central slot is simulated by using the finite-difference-method (FDM)-based Arbitrary-Lagrangian-Eulerian descriptions for the rigid oscillating body. The numerical procedure employs the second-order projection scheme to decouple the governing equations, and the multigrid algorithm with three levels to improve the computational efficiency in evaluating of the pressure equation. The present CFD method is validated through comparing the computed flutter derivatives of the ITP without slot to Theodorsen analytical solutions. Then, the unsteady aerodynamics of the ITP with and without central slot is investigated. It is found that even a smaller ratio of central slot of the ITP has notable effects on pressure distributions of the downstream section, and the pressure distributions on the downstream section will further be significantly affected by the slot ratio and the reduced wind speeds. Continuous increase of $A_2^*$ with the increase of central slot may be the key feature of the slotted ITP. Finally, flutter analyses based on the flutter derivatives of the slotted ITP are performed, and moreover, flutter instabilities of a scaled sectional model of a twin-deck bridge with various ratios of deck slot are investigated. The results confirm that the central slot is effective to improve bridge flutter stabilities, and that the flutter critical wind speeds increase with the increase of slot ratio.

Epithelial-mesenchymal Transition and Its Role in the Pathogenesis of Colorectal Cancer

Zhu, Qing-Chao,Gao, Ren-Yuan,Wu, Wen,Qin, Huan-Long Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Epithelial-to-mesenchymal transition (EMT) is a collection of events that allows the conversion of adherent epithelial cells, tightly bound to each other within an organized tissue, into independent fibroblastic cells possessing migratory properties and the ability to invade the extracellular matrix. EMT contributes to the complex architecture of the embryo by permitting the progression of embryogenesis from a simple single-cell layer epithelium to a complex three-dimensional organism composed of both epithelial and mesenchymal cells. However, in most tissues EMT is a developmentally restricted process and fully differentiated epithelia typically maintain their epithelial phenotype. Recently, elements of EMT, specially the loss of epithelial markers and the gain of mesenchymal markers, have been observed in pathological states, including epithelial cancers. Increasing evidence has confirmed its presence in human colon during colorectal carcinogenesis. In general, chronic inflammation is considered to be one of the causes of many human cancers including colorectal cancer(CRC). Accordingly, epidemiologic and clinical studies indicate that patients affected by ulcerative colitis and Crohn's disease, the two major forms of inflammatory bowel disease, have an increased risk of developing CRC. A large body of evidence supports roles for the SMAD/STAT3 signaling pathway, the NF-kB pathway, the Ras-mitogenactivated protein kinase/Snail/Slug and microRNAs in the development of colorectal cancers via epithelial-tomesenchymal transition. Thus, EMT appears to be closely involved in the pathogenesis of colorectal cancer, and analysis refered to it can yield novel targets for therapy.

Selection and optimization of nutritional risk screening tools for esophageal cancer patients in China

Wen Dong,Xiguang Liu,Shunfang Zhu,Di Lu,Kaican Cai,Ruijun Cai,Qing Li,Jingjing Zeng,Mei Li 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.1

BACKGROUND/OBJECTIVES: Malnutrition has multiple impacts on surgical success, postoperative complications, duration of hospital stay, and costs, particularly for cancer patients. There are various nutrition risk screening tools available for clinical use. Herein, we aim to determine the most appropriate nutritional risk screening system for esophageal cancer (EC) patients in China. SUBJECTS/METHODS: In total, 138 EC patients were enrolled in this study and evaluated by experienced nurses using three different nutritional screening tools, the Nutrition Risk Screening 2002 tool (NRS2002), the Patient-generated Subjective Globe Assessment (PG-SGA), and the Nutrition Risk Index (NRI).We compared sensitivity, specificity, positive and negative likelihood ratios, and Youden index generated by each of the three screening tools. Finally, cut-off points for all three tools were re-defined to optimize and validate the best nutritional risk screening tool for assessing EC patients. RESULTS: Our data suggested that all three screening tools were 100% sensitive for EC patients, while the specificities were 44.4%, 2.96%, and 59.26% for NRS 2002, PG-SGA, and NRI, respectively. NRI had a higher positive likelihood ratio as well as a higher area under the receiver operating characteristic curve compared to those of NRS 2002 and PG-SGA; although, all three tools had null negative likelihood ratios. After adjusting the cut-off points, the specificity and accuracy for all tools were significantly improved, however, the NRI remained the most appropriate nutritional risk screening system for EC patients. CONCLUSIONS: The NRI is the most suitable (highest sensitivity and accuracy) nutritional risk screening tool for EC patients. The performance of the NRI can be significantly improved if the cut-off point is modified according to the results obtained using MedCalc software.

A Color-Reaction-Based Biochip Detection Assay for RIF and INH Resistance of Clinical Mycobacterial Specimens

( Wen Fei Xue ),( Jing Fu Peng ),( Xiao Li Yu ),( Shu Lin Zhang ),( Boping Zhou ),( Dan Qing Jiang ),( Jian Bo Chen ),( Bing Bing Ding ),( Bin Zhu ),( Yao Li ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1

The widespread occurrence of drug-resistant Mycobacterium tuberculosis places importance on the detection of TB (tuberculosis) drug susceptibility. Conventional drug susceptibility testing (DST) is a lengthy process. We developed a rapid enzymatic color-reaction-based biochip assay. The process included asymmetric multiplex PCR/templex PCR, biochip hybridization, and an enzymatic color reaction, with specific software for data operating. Templex PCR (tem- PCR) was applied to avoid interference between different primers in conventional multiplex- PCR. We applied this assay to 276 clinical specimens (including 27 sputum, 4 alveolar lavage fluid, 2 pleural effusion, and 243 culture isolate specimens; 40 of the 276 were non-tuberculosis mycobacteria specimens and 236 were M. tuberculosis specimens). The testing process took 4.5 h. A sensitivity of 50 copies per PCR was achieved, while the sensitivity was 500 copies per PCR when tem-PCR was used. Allele sequences could be detected in mixed samples at aproportion of 10%. Detection results showed a concordance rate of 97.46% (230/236) in rifampicin resistance detection (sensitivity 95.40%, specificity 98.66%) and 96.19% (227/236) in isoniazid (sensitivity 93.59%, specificity 97.47%) detection with those of DST assay. Concordance rates of testing results for sputum, alveolar lavage fluid, and pleural effusion specimens were 100%. The assay provides a potential choice for TB diagnosis and treatment.

Synthesis and In Vitro Cytotoxicity of Cis-[Pt(NH3)(NH2OH)Cl2]

Qing-Song Ye,Xi-Zhu Chen,Yao Yu,Qiao-Wen Chang,Shu-Qian Hou,Wei-Ping Liu 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.6

A novel mixed NH3/NH2OH platinum(II) complex cis-[Pt(NH3)(NH2OH)Cl2] was synthesized and characterized by elemental analysis, FAB-MS, FT-IR and 1H NMR spectroscopy. This complex was determined to have a good water-solubility and satisfactory stability. The pertinent complex was evaluated for its in vitro cytotoxicity against 3AO, HCT-116, LNcap, A549/ATCC and SGC-7901 human carcinoma cell lines. It shows appreciable cytotoxic activity that is comparable with cisplatin and is much more active than carboplatin.

Comparison of the Effects of Propofol and Midazolam on Inflammation and Oxidase Stress in Children with Congenital Heart Disease Undergoing Cardiac Surgery

Wen-fang Xia,Qing-shan Zhou,Yu Liu,Qi-zhu Tang,Han-dong Zou 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.2

Purpose: To investigate and compare the effects of propofol and midazolam on inflammation and oxidase stress in children with congenital heart disease undergoing cardiac surgery. Materials and Methods: Thirty-two ASA class I-II children with congenital heart disease undergoing cardiac surgery were randomly divided into two groups: propofol combined with low dose fentanyl (PF group, n = 16) and midazolam combined with low dose fentanyl (MF group, n = 16). Tracheal extubation time and length of Intensive Care Unit (ICU) stay were recorded. Blood samples were taken before operation (T_0), at 2 h after release of the aorta cross-clamp (T_3) and at 24 h after operation (T_4) to measure interleukin 6 (IL-6), IL-8, superoxide dismutase (SOD) and malondialdehyde (MDA) levels. Myocardium samples were collected at 10-20 min after aorta cross-clamp (T_1) and at 10-20 min after the release of the aorta cross-clamp (T_2) to detect heme oxygenase-1 (HO-1) expression. Results: Tracheal extubation time and length of ICU stay in PF group were significantly shorter than those of the MF group (p < 0.05, respectively). After cardiopulmonary bypass, IL-6, IL-8 and MDA levels were significantly increased, and the SOD level was significantly reduced in both two groups, but PF group exhibited lower IL-6, IL-8 and MDA levels and higher SOD levels than the MF group (p < 0.05, respectively). The HO-1 expression in the PF group was significantly higher than that in MF group at the corresponding time points (p < 0.05, respectively). Conclusion: Propofol is superior to midazolam in reducing inflammation and oxidase stress and in improving post-operation recovery in children with congenital heart disease undergoing cardiac surgery.

XPD Lys751Gln and Asp312Asn Polymorphisms and Susceptibility to Skin Cancer: A Meta-Analysis of 17 Case-control Studies

Zhu, Hai-Li,Bao, Ji-Ming,Lin, Pei-Xin,Li, Wen-Xia,Zou, Zhen-Ning,Huang, Ye-En,Chen, Qing,Shen, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: Numerous studies have explored the influence of XPD Lys751Gln and/or Asp312Asn polymorphisms on skin cancer susceptibility. However, the results remain inconclusive. To derive a more precise estimation, we conducted a comprehensive search to identify all available published studies and performed a meta-analysis. Materials and Methods: Electronic literature searches of the PubMed, CBM and CNKI databases were performed up to March 2014. Odds ratios (ORs) with 95% confidence intervals (CIs) were applied to assess the strength of associations. Results: Seventeen case-control studies were included with a total sample size of 6, 113 cases and 11, 074 controls for the XPD Lys751Gln polymorphism, and 10 studies (3, 840cases and 7, 637 controls) for the XPD Asp312Asn polymorphism were pooled for analysis. Overall, no significant associations were found between the XPD Lys751Gln polymorphism and skin cancer risk in any genetic model. On stratified analysis by tumor type, XPD Lys751Gln polymorphism was not associated with increased risk of non-melanoma skin cancer, but was significantly related with increased risk of cutaneous melanoma (Gln/Gln vs Lys/Lys: OR=1.15, 95%CI=1.02-1.29, p=0.023; dominant model: OR=1.09, 95%CI=1.01-1.18, p=0.036). For the XPD Asp312Asn polymorphism, no significant association with skin cancer risk was observed in overall or subgroup analyses. Conclusions: The present meta-analysis suggests that the XPD Lys751Gln polymorphism may contribute to the risk of cutaneous melanoma from currently available evidence. Further investigations are needed to obtain more insight into possible roles of these two polymorphisms in skin carcinogenesis.

CFD based simulations of flutter characteristics of ideal thin plates with and without central slot

Zhi-wen Zhu,Zheng-qing Chen,Ming Gu 한국풍공학회 2009 Wind and Structures, An International Journal (WAS Vol.12 No.1

In this paper, the airflow around an ideal thin plate (hereafter referred to as ITP) with various ratios of central slot is simulated by using the finite-difference-method (FDM)-based Arbitrary-Lagrangian-Eulerian descriptions for the rigid oscillating body. The numerical procedure employs the second-order projection scheme to decouple the governing equations, and the multigrid algorithm with three levels to improve the computational efficiency in evaluating of the pressure equation. The present CFD method is validated through comparing the computed flutter derivatives of the ITP without slot to Theodorsen analytical solutions. Then, the unsteady aerodynamics of the ITP with and without central slot is investigated. It is found that even a smaller ratio of central slot of the ITP has notable effects on pressure distributions of the downstream section, and the pressure distributions on the downstream section will further be significantly affected by the slot ratio and the reduced wind speeds. Continuous increase of with the increase of central slot may be the key feature of the slotted ITP. Finally, flutter analyses based on the flutter derivatives of the slotted ITP are performed, and moreover, flutter instabilities of a scaled sectional model of a twin-deck bridge with various ratios of deck slot are investigated. The results confirm that the central slot is effective to improve bridge flutter stabilities, and that the flutter critical wind speeds increase with the increase of slot ratio.

A Comparative Study of Camouflage Printing Color Matching Based on Monitor and Paper Card

Yu-wen Wang,Qing-zhu Yi,Yi Ding,Guang-xin Wu,Jing-bin Zhang,Ni Wang 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4

To improve the accuracy of color reproduction for camouflage printing, a new color matching method, namedmonitor-paper-fabric, was proposed by importing color management into textiles, which matched the printing pastesaccording to the color of the paper card printed by the ink-jet printer after color management. Not like the traditional colormatching method, it matched the printing pastes according to the color from computer monitor after color correction. Twocolor matching methods were analyzed by comparing the color difference. It was found that the “monitor-paper-fabric” colormatching method could be considered as a convenient and feasible color matching method. Most color differences betweenthe monitor and the fabric in the camouflage pattern were reduced to lower than 4, except for color blocks 5 and 6. Inaddition, the color differences of six color blocks between the paper and the fabric were all less than 3.5, and were lower thanthose between the monitor and the fabric. The color consistency between the paper and the fabric was better than thatbetween the monitor and the fabric.