보체계와 항보체 활성 다당

양한철,신광순,성하진 高麗大學校自然資源科學硏究所 1993 自然資源科學硏究 Vol.1 No.-

일 농촌지역의 우울 증상에 관한 역학연구 -위험요인 및 요인구조분석

하양숙,홍진표,김한규,이군희,한경자,조맹제,김용익,송미순,박성애,김창윤 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.2

연구목적 : 세계적으로 널리 사용되는 우울증 척도인 the Center for Epidemiologic Studies Depression Scale(CES-D)을 이용하여 지역사회 주민의 우울증상의 정도를 평가하고, 우울증상의 위험요인을 분석하고, 한국인에서 우울증상의 요인구조를 알기 위해 본 연구를 실시하였다. 방 법 : 경기도 일 농촌 지역의 30세 이상 주민에 대해 무작위 군집 집락 추출을 실시하였고, 훈련된 면담원들이 가정 방문을 통하여 면접 및 자기보고 검사인 CES-D를 실시하였다. 총 1,315명에서 응답을 하였고 응답율은 59.3%이었다. 결 과 : CES-D에 의한 우울증 점수는 0∼5점이 57%정도를 차지하였고, 평균 점수는 남자 7.8점 여자 9.5점으로 비교적 낮은 편이었다. ANCOVA 분석 결과 비결혼, 저학력, 여성이라는 위험요인은 독립적으로 우울증상을 악화시키는 요인이었으나 연령은 통계적으로 유의하지 않았다. CES-D 결과를 요인분석을 시행한 결과 4가지 요인이 추출되었는데 우울증상 및 신체증상이 한 개의 요인으로, 다른 나라에서 보고되지 않는 정서적 고통이 별도의 요인으로 추출되었다. 결 론 : 국내 역학 조사에서도 외국과 유사한 우울점수 및 위험요인이 확인되었으나 우울증의 요인에서는 특이한 요인이 추출되었다. 한국인에 독특한 우울 반응에 관한 추가 연구가 필요하다. Objectives :The rates, correlates, and factor structure of depressive symptoms in a Korean rural sample were examined using Korean version of the Center for Epidemiologic Studies Depression Scale(CES-D). Methods : Door to door visiting survey was conducted by trained interviewers. A random cluster sample of 1,315 respondents, aged 30 and over completed the CES-D scale along with additional sociodemographic questionnaire. Results : In this sample, mean CES-D score of male and female were 7.8 and 9.5, respectively which were lower than those reported previously. Symptoms of depression were most common among the less educated, non-married and female according to ANCOVA. Age was not a significant risk factor for depressive symptoms. Different factor structures emerged from this sample as compared to other studies using this approach. Somatic symptoms and affective symptoms were combined as one factor and emotional hardship emerged as a separate factor. Conclusion : Symptoms scores and risk factors were similar to the results reported in other countries but unique factor structures were found in this study. Further research is needed about the Korean depressive symptomatology and related areas.

삼색도장벗(Daedaleopsis tricolor)P3에 의한 염료의탈색

윤경하,최양순 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

The present research was undertaken to investigate the activity of ligninolytic enzymes and the decolorization capability of some dyes with Daedaleopsis tricolor P3, isolated from nature and identified. For the assay of enzyme activities, the isolate produced lignin peroxidase(LiP), veratryl alcohol oxidase(VAO), laccase and manganese dependent peroxidase(MnP). At first, laccase and VAO were secreted as early as on day 2, but laccase activity was higher than VAO, and increased gradually, peaking on day 6 (89.1nmol/mg·protein). While LiP appeared on day 5 later than MnP, and increased significantly peaking on day 7 (50.4nmol/mg·protein). When the isolate was incubated with each dye for 10 days, the decolorization rates of azo dyes, such as orange Ⅱ, orange G, tropaeolin O and congo red were 90.7%, 89.7%, 88.4% and 82.4%, respectively. In case of heterocyclic dyes, eosin Y, toludine blue, methyl blue and azur B were 97.7%, 96.4%, 98.3% and 92.7% respectively. Finally those of triphenylmethane dye such as basic fuchsin, malachite green and crystal violet were 98.2%, 93.6% and 99.0%, respectively. This results suggest that laccase and VAO of Daedaleopsis tricolor P3 should be played an important role in the decolorization of the dyes.

구름버섯(Coriolus versicolor) VK7 에 의한 Anthracene, Phenanthrene 및 Benzo(α)pyrene 의 분해

윤경하,최양순 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.1

The present research was undertaken to investigate the activity of ligninolytic enzymes and the biodegradation capability of anthracene, phenanthrene and benzo(α)pyrene with Coriolus versicolor VK7, isolated from nature and identified. For the assay of enzyme activities, the isolate did not produce lignin peroxidase (LiP) and veratryl alcohol oxidase (VAO), but laccase and manganese dependent peroxidase (MnP). While the activity for MnP was low (22.2 nmol/mgㆍprotein), its laccase activity was very high (740.0 nmol/mgㆍprotein). Moreover, laccase had appealed earlier than MnP. When the isolate was incubated with 10mg/L (final concentration) of anthracene, phenanthrene and benzo(α)pyrene, the isolate removed anthracene 74.1%, 86.1% and 100%, respectively for 7, 14 and 28 days incubation. In case of phenanthrene was 70.3%, 89.2% and 100%, respectively. Finally the results of benzo(α)pyrene was 58.3%, 64.8% and 84.8%, respectively. The resells suggest that laccase of Coriolus versicolor VK7 should play an important role in the biodegradation of these PAHs compounds.

송곳니구름버섯(Irpex zonatus) BN2에 의한 다핵방향족 탄화수소화합물의 분해

최양순,윤경하 순천향대학교 기초과학연구소 1996 순천향자연과학연구 논문집 Vol.2 No.2

우리 나라 자연 환경에서 분리 동정된 송곳니구름버섯 (Irpex zonatus) BN2 균주의 리그닌 분해효소활성과 anthracene, phenanthrene 및 benzo(a)pyrene의 분해능을 조사하였다. 송곳니구름버섯 BN2 균주는 lignin peroxidase (LiP) 와 veratryl alcohol oxidase (VAO)를 생산하지 않고 laccase와 manganese dependent peroxidase (MnP)를 생산했다. MnP는 배양 3일부터 생산되었으나 효소활성은 매우 낮았다. 반면 laccase는 배양 초부터 지속적으로 생산되었고 활성은 대단히 높았다. 균주를 PAHs 화합물에 접종하여 배양했을 때 anthracene 분해율은 배양 1주에서 74.6%, 2주에서 92.9%, 4주에서 100%의 분해율을 접종하여 나타났다. phenanthrene 의 경우 배양 1주와 2주에서 각각 74.3%와 91.6%의 분해율을 나타냈고 4주에서 100%의 분해율을 나타냈다. benzo(a)pyrene 은 각각 41.8%, 49.0% 및 82.5%의 분해율을 나타내서 다른 PAHs 화합물보다 비교적 낮은 분해율을 보였다. 본 실험 결과 송곳니구름버섯 BN2 균주에 의한 PAHs 화합물의 분해는 주로 laccase에 의하여 이루어진다고 생각된다. The present research was undertaken to investigate the activity of ligninolytic enzymes and the biodegradation capability of polycyclic aromatic hydrocarbons (PAHs) with Irpex zonatus BN2, isolated from nature and identified. For the assay of enzyme activities, the isolate did not produce lignin peroxidase (LiP) and veratryl alcohol oxidase (VAO), but laccase and manganese dependent peroxidase (MnP). While the activity for MnP was low (61.6 n㏖/㎎·protein). its laccase activity was very high (1185.9 n㏖/㎎·protein). Moreover. laccase had appeared earlier than MnP When the isolate was incubated with 10㎎/L (final concentration) of anthracene, phenanthrene and benzo(a)pyrene, the isolate removed anthracene 74.6%, 92.9%, and 100% respectively for 7,14 and 28 days incubation. In case of phenanthrene was 74.3%, 91.6% and 100% respectively. Finally the results of benzo(a)pyrene was 41.8%, 49.0% and 82.5%, respectively. The results suggest that laccase of Irpex zonatus BN2 should play an important role in the biodegradation of these PAHs compounds.

한국인 본태성 고혈압환자에서 Angiotensinogen M235T 유전자 다양성

양동호(Dong Ho Yang),홍세용(Sea Yong Hong),김홍수(Hong Soo Kim),방차옥(Cha Ok Bang),김철현(Chul Hyun Kim),최종순(Jong Soon Choi),하권수(Kwun Soo Ha) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.3

N/A Background: Potential involvement of the angio-tensinogen gene(M235T) in the pathogenesis of essen-tial hypertension has been suggested by some inves- tigators. However, an association between M235T gene polymorphism and essential hypertension has been reported by some, but not by others. Since genetic diversity exists among different ethnic population, we addressed the question of whether there is an association between M235T gene poly-morphism and essential hypertension in the Korean. Methods: 100 patients with essential hypertension and 100 control subjects were recruited from out- patients at the Department of Internal Medicine, Soon-chunhyang Hospital, Chunan City. The criteria for hypertension was defined as systolic blood pressure higher than 160mmHg and/or diastolic blood pressure higher than 95mmHg. For detection of the M235T polymorphism at the angiotensinogen locus, the primer sequences were: sense primer : 5'-TGAAGGAG- AAGGTGTCTGCGGGA-3' and antisense primer : 5'- AGGACGGTGCGGTGAGAGTG-3'. The PCR product mixture was exposed to restriction enzyme Tthlll I and then submitted to electrophoresis in polyacryl-amide gel. Differences between the molecular variants of the gene in hypertensives and normotensives were com- pared by using the X²test. p<0.05 was considered statistically significant. The odds ratio and 95% confidence interval were calculated using Woolf's method. Results: Compared with the control subjects, hypertensives had higher values of three established risk factors for hypertension : age, BMI cholesterol (total and LDL). X analysis showed no difference in the distribution of genotype or allele frequency between the hypertensives and normotensives(X²=1.14, p=0.29). The crude odds ratio was 0.73 for CC over CT(95% confidence interval 0.41-1.30). The adjusted odds ratio with age, sex, lipid profilcs and BMI was 0.68 for CC over CT(9596 confidence interval 037-1,23). Conclusion : The molecular varient M235T of the angiotensinogen gene is not associated with essential hypertension in Korean population.

베르누이 정리를 이용한 혈액 투석관내 혈류량 측정법 개발

이종훈,송순영,조현경,윤수영,양성자,허규하,김순일,김유선,박기일 대한혈관외과학회 2004 Vascular Specialist International Vol.20 No.1

Wolff-Parkinson-White증후군의 임상상 및 전기생리학적 소견

안신기,이문형,장양수,오동진,최인석,하종원,임세중,김병옥,정남식,심원흠,조승연,김성순 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.9

Selection of β-carotene enhanced transgenic soybean containing single-copy transgene and analysis of integration sites

Yang Qin,Soon-Jong Kweon,Young-Soo Chung,Sun-Hwa Ha,Kong-Sik Shin,Myung-Ho Lim,Taek-Ryoun Kwon,Soon Ki Park,Hyun-Suk Cho,Hee-Jong Woo 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

The β-carotene biofortified transgenic soybean was developed recently through Agrobacterium -mediated transformation using the recombinant PAC (Phytoene synthase-2A-Carotene desaturase) gene in Korean soybean (Glycine max L. cv. Kwangan). GM crops prior to use as food or release into the environment required risk assessments to environment and human health in Korea. Generally, transgenic plants containing a copy of T-DNA were used for stable expression of desirable trait gene in risk assessments. Also, information about integration site of T-DNA can be used to test the hypothesis that the inserted DNA does not trigger production of unintended transgenic proteins, or disrupt plant genes, which may cause the transgenic crop to be harmful. As these reasons, we selected four transgenic soybean lines expressing carotenoid biosynthesis genes with a copy of T-DNA by using Southern blot analysis, and analyzed the integration sites of their T-DNA by using flanking sequence analysis. The results showed that, T-DNA of three transgenic soybean lines (7-1-1-1, 9-1-2, 10-10-1) was inserted within intergenic region of the soybean chromosome, while T-DNA of a transgenic soybean line (10-19-1) located exon region of chromosome 13. This data of integration site and flanking sequences is useful for the biosafety assessment and for the identification of the β-carotene biofortified transgenic soybean.

Cervicovaginal Fetal Fibronectin and Ultrasonographic Examination of Cervix: Which is Better as a Predictor of Preterm Delivery in Patient with Preterm Labor and Intact Membranes?

( Soon Ha Yang ),( Chung Rae Roh ),( Jae Hyun Chung ),( Je Ho Lee ) 대한산부인과학회 1996 대한산부인과학회 학술대회 Vol.77 No.-