Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

[논문]실시간 정밀 3차원 지형측량기법을 이용한 저수량산출시스템 개발

송석진,장용구,김상석,강인준 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

현재 고정밀도 저수량 측량에 사용되는 측량장비에는 수평위치결정방법으로 실시간 DGPS측량장비가 많이 활용되고 있고, 수심측량에는 디지털 음향측심기를 많이 사용하고 있다. 본 연구에서는 현재, 수심측량 결과처리 시 문제가 되고 있는 소프트웨어의 가격부담을 줄이고 수심측량과 내업처리에 의한 저수량산출을 통합처리 할 수 있는 국내 자체개발의 저가형 외 · 내업 일체형 저수량산출시스댐을 개발하였다.

산업장 근로자의 표준체중치

정종학,강복수,김석범,사공준,이정길,한구웅 大韓産業醫學會 1989 대한직업환경의학회지 Vol.1 No.1

산업장 근로자의 건강증진과 노동력보존에 기여할 목적으로 경상북도 구미시에 소재하는 구미공단내의 20-29세 산업장 근로자 10,407명(남자 6,201명. 여자 4,206명)을 대상으로 1985년의 정기 신체 검사 자료로 부터 성별. 연령별 평균신장, 평균체중 및 신장과 체중간의 상관관계와 회귀방정식을 구하고 이들을 이용하여 신장별 표준체중과 이상체중의 범위를 구했으며 신장과 체중으로 BMI를 산출하였다. 20대 근로자의 평균신장은 남자가 168.2±5.61cm였고 여자는 155.9±5.26cm였고 평균체중은 남자가 61.4±6.56kg, 여자는 52.4±6.00kg이었다. 신장과 체중간의 상관계수는 남자의 경우 +0.541이었고 회귀방정식은 Y(체중)=0.637Ⅹ(신장)-44.975였으며 여자는 상관계수가 +0.559였고 회귀방정식은 Y(체중)=0.637 Ⅹ(신장)-46.898 이었다. 신장별 표준체중은 신장이 155cm일때에 남녀 각각 53.0kg, 51.8kg 이었으며 165 cm 일때는 59.3kg, 58.2kg이었고 175cm일때에 65.6kg, 64.6kg이었고, 정상체중의 범위는 신장이 155cm일때에 남자는 47.5-58.5kg, 여자는 46.9-56.8kg, 165cm일때는 남자는 53.8-64.8kg, 여자는 53.2-63.2kg, 175cm일때에 남자는 60.1-72.1kg, 여자는 59.6-69.6kg으로 평가되었으며, 비만은 신장이 155cm 일때에 남자는 64.1kg이상, 여자는 61.8kg이상, 165cm일때는 남자는 70.3kg이상, 여자는 68.2kg이상, 175cm일때에 남자는 76.7kg이상, 여자는 74.5kg 이상으로 평가되었다. 성별. 연령별 BMI는 남자가 21.7±1.95였고 여자는 21.6±2.05였고 Garrow의 BMI 분류방식에 따른 desirable range에 속하는 근로자는 남자가 75.9%였고, 여자는 71.3%였다. To contribute to promotion of health and preservation of labor power of Korean laborers, the body height and body weight were measured for 10,407 workers(6,201 male, 4,206 female) in the age group of 20-29, engaged in manufactures in the Gumi industrial complex, Gumi city, Kyungpook province. The above data were extracted from the 1985 periodic examination chart for calculation of the mean body weight, mean body height, correlation coefficient and regression equation between weight and height, standard body weight, body mass index(BMI) and distribution of laborers within Garrow's classification of BMI by age and sex group. Mean body height of 20-29 age group was 168.2±5.61 cm for male and 155.9±5.26 cm for female. Mean body weight of 20-29 age group was 61.4±6.56 kg for male and 52.4±6.00 kg for female. Correlation coefficient and regression equation of 20-29 age group were +0.541 and Y(Wt)=0.632X(Ht)-44.975 for male and +0.559 and Y(Wt)=0.637X(Ht)-46.898 for female. Standard body weight of 20-29 age group was 53.0 kg at 155 cm, 59.3 kg at 165 cm, 65.6 kg at 175 cm for male and 51.8 kg at 155 cm, 58.2 kg at 165 cm at 64.6 kg at 175 cm for female. Range of normal body weight of 20-29 age group was 47.5-58.5 kg at 155 cm, 53.8-64.8 kg at 165 cm, 60.1-72.1 kg at 175 cm for male and 46.9-56.8 kg at 155cm, 53.2-63.2 kg at 165 cm, 59.6-69.6 kg at 175 cm for female. Range of obesity of 20-29 age group was 64.1 kg and over at 155 cm, 70.3 kg and over at 165 cm, 76.7 kg and over at 175 cm for male and 61.8 kg and over at 155 cm, 68.2 kg and over at 165 cm, 74.5 kg and over at 175 cm for female. Body mass index(kg/m²) of 20-29 age group was 21.7±1.95 for male and 21.6±2.05 for female, 75.9% of male laborers and 71.3% of famale counterparts fall in the desirable range of BMI by Garrow's classification.

고빌리루빈혈증을 동반한 자가면역성 간염 1례

서영범,김성욱,장재식,강혁주,이중현,윤병구,김욱년,이광헌,이구,유석동,양창헌,이정호,이영현,이창우,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

자가면역성 간염은 대개 만성 경과를 가지며, 혈중 자가면역항체와 혈청 글로불린치의 상승, 그리고 조직학적으로 괴사 염증성 변화를 특징으로 하는 질환으로 아직 정확한 병인이 밝혀져 있지 않은 상태이다. 발병연령은 대개 젊은 영자(15-25세)에서 호발한다. 이 질환은 급성 간염의 임상경과를 보일 수 있으나, 심한 급성 간염이나 전격성 간염으로도 나타날 수 있는데, 이 경우 아주 나쁜 예후를 보인다고 한다. 치료는 자가면역성 간염 임상 경과의 다양성이나 병인, 병리기전의 불확실성에도 불구하고 대개 steroid 치료에 반응하는 것으로 알려져 있으며 대개 80%의 관해율을 나타내며 궁극적으로 간경변으로의 진행을 막을 수 있는 것으로 보인다. 저자들은 58세 남자에서 발생한 급성의 경과를 가지고 심한 황달을 동반한 자가면역성 간염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Autoimmune hepatitis (AIH) is a chronic necroinflammatory liver disorder of unknown cause associated with circulating autoantibodies and a high serum globulin level. The age of onset of AIH show a peak between the age of 15 and 25 years. AIH can develop and be manifested as acute hepatitis, but severe form of acute hepatitis or fulminant hepatic failure has a poor prognosis. Although AIH is likely to progress from chronic active hepatitis to cirrhosis, steroid therapy can control the disease activity, prolong survival, improve the quality of life , and defer liver transplantation. In the present report we describe a 58-year-old man who admitted because of progressive jaundice and fatigue. He was diagnosed with AIH from laboratory test result showing positivity for antinuclear antibodies, anti-smooth muscle antibodies, and negativity for hepatitis viral markers and from liver biopsy. Steroid therapy, oral administration of prednisolone, was effective in improving the liver function test. Following liver biopsy 6 months after onset shows markedly improved necroinflammatory activity.

HCC : Prognostic Significance of p53, mTOR, c-Met, IGF1R and HSP70 Overexpression after Hepatic Resection of Hepatocellular Carcinoma

( Gu Hyum Kang ),( Byun Seok Lee ),( Dae Young Kang ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: With new discoveries in cancer biology, the pathological and biological prognostic factors of hepatocellular carcinoma (HCC) have been studied extensively. Molecular biomarkers not only might predict prognosis for patients with HCC but also may provide targets for potential therapeutic agents. However, simultaneous study about variable molecular biomarker is rare in South Korea. The aim of the present study was to investigate the clinicopathological significance of the expression of p53, mTOR, c-Met, IGF1R and HSP70 in surgically resected HCC. Methods: From the database of the department of pathology of Chungnam National University Hospital, 83 cases of radically resected HCCs from January 1999 to December 2011 were recruited for this study. Clinical information was reviewed respectively through electric medical records.We analyzed immunohistochemical expression of p53, mTOR, c-Met, and IGF1RB, HSP70 with patient clinicopathologic feature such as sex, age, liver cirrhosis, tumor size, Edmondson grade, microvascular invasion, portal vein invasion, hepatic artery invasion, and survival. Results: p53 was expressed higher in undifferentiated histological tumor grade (Edmondson G3/G4 vs. G1/G2), cirrhosis, portal vein invasion and male. High-expression of c-MET correlated with cirrhosis and high-expression of mTOR correlated with Edmondson grade, cirrhosis and high-expression of IGF1R correlated with Edmondson grade, cirrhosis. Multivariate analysis identified significant relationship between high expression of p53 and Edmondson grade, portal vein invasion. Additionally, high expression of mTOR related to Edmondson grade, cirrhosis and high expression of HSP70 related to portal vein invasion. The Kaplan Meier survival for patient with high vs low Edmondson grade (66.5% vs. 93.3%, P value=0.012) as well as p53 expression (63.0% vs. 85.6%, P value=0.058) was statistically significant. Conclusions: Correlation between molecular biomarker and Histological grade possibly account for pathogenesis of hapatocelluar carcinoma. Histological grade correlated with survival. Molecular markers such as p53, mTOR and IGF1R predict malignant behavior of HCCs. p53 and mTOR were independent prognostic indicators. Our result show p53 is most potent molecular predictor for carcinogenesis and malignancy from other markers. Further study is needed regarding HSP70 as a prognostic indicator.

Original Article : Prognostic Significance of p53, mTOR, c-Met, IGF-1R, and HSP70 Overexpression after the Resection of Hepatocellular Carcinoma

( Gu Hyum Kang ),( Byung Seok Lee ),( Eaum Seok Lee ),( Seok Hyun Kim ),( Heon Young Lee ),( Dae Young Kang ) The Editorial Office of Gut and Liver 2014 Gut and Liver Vol.8 No.1

Background/Aims: The current study examines the expression of molecular biomarkers in hepatocellular carcinoma (HCC) and whether these findings correlate with the clinicopathologic features of the disease and patient survival. Methods: We analyzed the immunohistochemical expression of p53, mammalian target of rapamycin (mTOR), c-Met, and insulin-like growth factor 1 receptor (IGF-1R) heat shock protein 70 (HSP70) with the clinicopathologic features of 83 HCCs. Results: p53 expression was higher in the male patients with undifferentiated histological tumor grades, cirrhosis, and portal vein invasion. High 48 c-Met expression correlated with cirrhosis, and high mTOR expression correlated with the tumor grade and cirrhosis. High IGF-1R expression correlated with the tumor grade and cirrhosis. A multivariate analysis identified a significant relationship between the high expression of p53, tumor grade, and portal vein invasion. In addition, a high expression of mTOR was related to tumor grade and cirrhosis, and a high expression of HSP70 was related to portal vein invasion in a multivariate analysis. The Kaplan-Meier survival curve for patients with high versus low Edmondson grades and p53 expression was statistically significant. Conclusions: p53, mTOR, and IGF-1R expression correlated with the Edmondson tumor grade in a univariate analysis, while p53 and mTOR correlated with the Edmondson tumor grade in a multivariate analysis. In addition, the tumor grade was found to predict survival. p53 was primarily related to the clinicopathologic features compared to other markers, and it is a poor prognostic factor of survival. (Gut Liver 2014,8:79-87)

The polypoid ganglioneuroma associated with hyperplastic polyposis

( Gu Hyum Kang ),( Byung Seok Lee ),( Dae Young Kang ),( Hoon Choi ) 대한내과학회 2016 The Korean Journal of Internal Medicine Vol.31 No.4

Topological and Statistical Analysis for the High-Voltage Transmission Networks in the Korean Power Grid

Seok-Gu Kang,Sung-Guk Yoon 한국통신학회 2017 韓國通信學會論文誌 Vol.42 No.4

A case of primary adenosquamous carcinoma of the liver

Gu Hyum Kang,Byung Seok Lee,Dae Young Kang 한국간담췌외과학회 2013 한국간담췌외과학회지 Vol.17 No.1

Adenosquamous carcinoma (ASC) is a rare tumor of the liver, which carries a poor prognosis. It has been reported mostly in the form of case studies. Recently, we experienced a case of primary ASC of the liver that has been preoperatively considered as cholangiocelluar carcinoma of the liver. Microscopically, the tumor was composed of adenocarcinoma, squamous cell carcinoma, as well as transitional area, including the transformation of adenocarcinoma to squamous cell carcinoma. The patient remained well with no evidence of post-resection complication or recurrence for 15 month.