대추 아임계수 추출물의 폴리페놀 함량 및 산화방지 평가

고민정(Min-Jung Ko),권미리(Mi-Ri Kwon),정명수(Myong-Soo Chung) 한국식품과학회 2021 한국식품과학회지 Vol.53 No.3

아임계수 추출 기술을 이용하여 대추 추출물을 얻고 총 페놀 함량, DPPH 라디칼 소거능과 ABTS 라디칼 소거능을 이용하여 추출조건에 따른 페놀 성분 함량 변화 및 산화 방지효과를 확인하였다. 아임계수 추출물은 총 페놀 함량, DPPH라디칼 소거능, ABTS 라디칼 소거능의 결과 값이 모두 메탄올 및 에탄올을 이용하여 추출한 결과 보다 우수하게 나타났다. 아임계수 추출법은 메탄올 및 에탄올을 이용하여 추출하는 방법 보다 추출 시간도 20분 이내로 짧았고, 유기용매를 사용하지 않고 친환경적으로 추출할 수 있었다. 아임계수 추출물의 총 페놀 함량과 산화방지 효과의 결과는 높은 상관관계를 보였는데, 항산화 효과가 있는 페놀류가 많이 추출될수록 산화방지 효과도 우수하게 나타났다. 본 연구를 통해, 아임계수 추출 방법은 오직 물을 사용하여 유효성분을 추출하는 친환경적이고 안전한 신가공기술이며 잠재성과 우수성을 확인하였다. 아임계수 추출기술은 앞으로도 다양한 유효성분추출 및 소재 가공으로 식품 산업에 응용하여 사용될 수 있을 것이다. Subcritical water extraction (SWE) is an eco-friendly extraction method that uses only purified water as a solvent under high temperature and high pressure conditions. In this study, total phenolics, and antioxidant activity were evaluated in Ziziphus jujuba Mill extracts from subcritical water obtained by varying the extraction temperature (110-190℃) and extraction time (1-20 min). Total phenolics was maximized with extraction at 190℃ for 15 min (67.79±3.45 mg gallic acid equivalent/g jujube). The 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity (48.84±4.74%) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free radical scavenging activity (84.75±1.15%) were maximal at extraction conditions of 190℃, for 20 min. All jujube extracts prepared using SWE had higher total phenolics and antioxidant activities than extracts prepared using organic solvent extraction (60℃, 120 min), including methanol and ethanol. SWE could be an excellent alternative to organic solvents for extracting phenolics and antioxidant compounds.

Effects of hydrogen plasma treatment on SnO2:F substrates for amorphous Si thin film solar cells

Min-Seung Choi,Young-Ju Lee,Jung-Dae Kwon,Yongsoo Jeong,Ju-Yun Park,강용철,Pung Keun Song,Dong-Ho Kim 한국물리학회 2013 Current Applied Physics Vol.13 No.8

We investigated the effects of hydrogen plasma treatment on the physical and electrical properties of fluorine-doped tin oxide (FTO) films used for amorphous silicon (a-Si) thin film solar cells. A slight increase in carrier concentration by the hydrogen doping effect was observed for the FTO film exposed to the hydrogen plasma for 5 min. For further exposure to the plasma, the chemical reduction became prominent and resulted in deterioration of the electrical and optical properties of the film. XPS analysis revealed that the chemical reduction of SnO2 to Sn metallic state occurs on the surface region. It was found that the defects formed by hydrogen plasma act as recombination centers at the interface between FTO electrode and p-layer of a-Si solar cells. This phenomenon resulted in the deterioration of the cell performance. The averaged conversion efficiency (6.82%) of the cells on pristine FTO hydrogen substrate was decreased to 5.81% for the cells on FTO treated for 5 min, which is mainly attributed to the decrease in short-circuit current density.

Feasibility of Calibrating Smartphone to Access Physical Activity

이정민(Jung Min Lee),김영원(Young Won Kim),권이석(Yi Suk Kwon),Timothy R. Derrick,Gregory J. Welk 한국체육측정평가학회 2015 한국체육측정평가학회지 Vol.17 No.3

대부분의 신체활동측정을 위해 연구들은 가속도계를 이용하여 측정되어져 왔다. 하지만, 스마트폰이 대중화 되면서스마트폰에 내장된 가속도계를 이용하여 신체활동을 측정 할 수 있는 에플리케이션들이 빈도 높게 늘어나고 있다. 따라서 이 연구에서는 스마트폰에 내장되어 있는 가속도계를 이용하여 에너지 소비량을 구하는 공식을 만들고 그 공식을 현재 연구중심으로 많이 사용되는 가속계와 비교하고 교차분석을 통하여 정확성과 실용성을 검증하는 것이다. 이러한 목적을 달성하기 위하여 건강한 성인 44명을 편의추출 하여 가속계와 스마트폰을 착용시켜 69분 동안 다양한 일상생활 활동을 시켰으며 신체활동 측정의 준거검사인 간접열량계(Oxycon Mobile)가 사용되었다. 회귀분석을 통하여 만들어진 연산은 교차 타탕도 분석을 통하여 정확도를 검증하였고 검사도구의 정확성은 절대오차율(%)을 계산하고 검사도구간 상관계수 (r), Bland-Altman plot 과 equivalence testing 사용하여 검증하였다. 연구결과에 의하면 스마트폰을 통해 얻어진 절대오차율은 (42.4%)를 보였고 Freedson`s 기준치와 비교했을 때는 (38.2%)를 보였다. 그러나 스마트폰을 통해 만들어진 연산은 걷기 와 달리기의 에너지 소비량은 측정하는데 유의하다는 결과가 나왔다. 추후, 스마트폰만을 이용한 신체활동 측정이 다양하게 필요하고 그 정확성을 검증하는 연구들도 필수적으로 요구된다. The purpose of the study was to develop algorithms to predict energy expenditure (EE) and to evaluate their utility compared to existing accelerometer technology. Forty-four participants wore an Actigraph GT3X (AG3X) and the Samsung Galaxy S2 Smartphone (SP) over a 69 min period. Oxygen consumption (VO<sub>2</sub>) was simultaneously measured by a portable indirect calorimetry system. EE prediction equations for the SP were developed from the walking and running activities using standard regression methods. The equation was then cross-validated on a separate holdout sample (n=22) using equivalence testing to evaluate accuracy. The overall mean absolute percentage errors were large for both the SP algorithm (42.4%) and the Freedson`s AG3X algorithm (38.2%). However, the estimated METs from the SP was statistically equivalent to the measured METs for the two activities used in calibration: walking (90% CI: 2.85, 3.50 kcal·min-1) and running (90% CI: 7.64, 8.25 kcal·min-1). Other activities were not accurately assessed with the SP but none of the estimates from the AG3X met the statistical criteria for equivalency. The study demonstrates that data from SP can be easily calibrated to estimate EE and that accuracy is comparable to the common research-grade monitors.

난치성 백반증에 대한 흡입물집을 이용한 비배양 표피세포이식술 20례

배정민 ( Jung Min Bae ),정한미 ( Han Mi Jung ),이한나 ( Han Na Lee ),이로우 ( Ro Woo Lee ),은성혜 ( Sung Hye Eun ),권혁선 ( Hyuck Sun Kwon ),이지혜 ( Ji Hae Lee ),김경문 ( Gyong Moon Kim ) 대한피부과학회 2018 대한피부과학회지 Vol.56 No.7

Background: As nonsurgical interventions for vitiligo are not always successful, various surgical modalities have been used in patients with refractory vitiligo. Of these, non-cultured epidermal suspension transplantation (NCES) was recently introduced to treat large recipient sites using cells from small donor tissue. Objective: We assessed the effectiveness and safety of NCES as a surgical treatment for patients with refractory vitiligo. Methods: We retrospectively reviewed 20 cases in 17 patients (11 females; median age 25 years) who underwent NCES from July 2015 through March 2018. Suction blisters (20 mm in diameter) were collected from the patient’s inner thigh at a donor-to-recipient area ratio of 1:5. After the addition of 5 mL recombinant trypsin solution to the suction blisters, followed by incubation at 37°C for 60 min, epidermal cells were manually scraped off the blister surface, and epidermal cell suspension was obtained by centrifugation at 1,500 RPM for 5 min. The suspension was applied to the vitiligo regions after epidermal ablation of those regions. Phototherapy resumed 1 month later. Treatment success was defined as ≥75% repigmentation of the surgical site, and all adverse events were noted. Results: Overall, 85.0% of cases (17/20) exhibited treatment success. Adverse events included hyperpigmentation (20%) and surgical site infection (5%), but the treatment was tolerable in all cases. Conclusion: NCES is a reliable surgical option for patients with vitiligo refractory to nonsurgical treatment. Large areas of vitiligo can be treated by NCES, and use of this technique should be encouraged in Korea. (Korean J Dermatol 2018;56(7):426∼432)

자몽껍질 유래 플라바논의 최적 추출 및 기능성 소재 캡슐화

고민정(Min-Jung Ko),권혜림(Hye-Lim Kwon),정명수(Myong-Soo Chung) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.4

버려지는 자몽 껍질에 다량 함유되어있는 플라바논을 추출하여 기능성 성분을 재이용하였다. 친환경용매인 아임계수 추출기술을 이용하여 170℃, 10 min의 최적 조건에서 추출함으로써 무독성 용매로 빠르고 경제적으로 추출할 수 있었다. 자몽껍질 추출물을 β-cyclodextrin을 이용하여 처리함으로써 플라바논과 같은 비극성 물질을 캡슐화하여 소재의 가용화를 용이하게 하였다. 이것은 항산화 기능이 향상된 대체 소재의 개발 및 건강지향식품에 이용할 수 있으며, 이를 통하여 세계 기능성 식품 시장으로의 진출을 위한 발판으로도 삼을 수 있을 것으로 기대된다. The extraction of flavanones such as naringin, narirutin, naringenin, hesperidin, and hesperetin from grapefruit peels was performed using subcritical water extraction (SWE), hot water extraction, and conventional methods such as methanol and ethanol extraction. We analyzed the total flavanone content using high-performance liquid chromatography (HPLC) for each extracting method. Among the three methods, SWE was the optimal method with optimal operating conditions of 170℃ temperature and 10 min operating time. The maximum total flavanone extracted was 86.539±3.52 mg/g grapefruit peels. Moreover, we treated the extracts with 60% β-cyclodextrin and then analyzed the surface structure of the encapsulated compounds by field emission-scanning electron microscopy (FE-SEM). The results indicated that the encapsulation in β-cyclodextrin improved solubilization, and the inclusion complex could serve as food supplements.

스포츠복지문화 활성화를 위한 정책요인의 우선순위 산정

김민규 ( Kim Min-kyu ),박수정 ( Park Soo-jung ),박병권 ( Park Byung-kwon ) 인하대학교 교육연구소 2018 교육문화연구 Vol.24 No.4

본 연구는 스포츠복지문화 활성화를 위한 정책요인에 대해서 AHP를 활용하여 가중치를 도출하고, 향후 어떠한 정책요인부터 우선적으로 실현해야 하는지에 대한 제언하는데 목적이 있다. 이를 위하여 김민규와 송정란(2018)이 연구한 스포츠복지문화 활성화를 위한 정책요인들을 활용하여 우선순위 산정을 실시하였다. 이에 따른 주요 결론은 다음과 같다. 첫째, 계층 1은 정책 요인이, 둘째, 계층 2의 하위계층은 서비스전달체계 요인이, 셋째, 계층 2 중 정책 요인의 하위계층은 서비스전달체계 요인이, 넷째, 계층 2 중 인프라 요인은 콘텐츠웨어 인프라 요인이, 다섯째, 층 2 중 연구개발 요인은 전문가양성운영체계 요인이, 여섯째, 정책 요인의 계층 3 중 유관 법안 개정 요인은 관련 법안의 지원에 대한 논의 요인이, 일곱째, 정책 요인의 계층 3 중 정책개발 요인은 스포츠복지 정책사업 개발 요인이, 여덟째, 정책 요인의 계층 3 중 인식개선 요인은 지자체 및 유관기관의 복지적 관점 증진 요인이, 아홉째, 정책 요인의 계층 3 중 서비스전달체계 요인은 행정지원 부처 결정 및 역할 정립 요인이, 열 번째, 정책 요인의 계층 3 중 예산관련 요인은 예산확보방안 요인이, 열한 번째, 인프라 요인의 계층 3 중 하드웨어 인프라 요인은 기존 스포츠 시설 활용의 효율성 제고 요인이, 열두 번째, 인프라 요인의 계층 3 중 휴먼웨어 인프라 요인은 스포츠복지 강사충원 요인이, 열세 번째, 인프라 요인의 계층 3 중 콘텐츠웨어 인프라 요인은 스포츠복지 프로그램 개발 요인이, 열네 번째, 연구개발 요인의 계층 3 중 전문가 양성 운영체계 요인은 스포츠복지학의 교육과정 개발 요인이, 열다섯 번째, 연구개발 요인의 계층 3 중 기초자료 조사 요인은 스포츠와 복지 전문가의 상호교류 요인이, 열여섯 번째, 연구개발 요인의 계층 3 중 기획연구개발 요인은 스포츠복지 대체 프로그램 개발요인이 최우선 고려되어야 하는 것으로 분석되었다. 본 연구의 결과는 정책과정 전반과 학문의 유기적 결합에 도움이 될 것이다. 추후 학계와 현장의 의견 교류 및 비교분가 가능한 질적연구가 이루어져야 할 것이다. The purpose of this study is to identify the weight of the policy factors by using AHP for the purpose of activating sports welfare culture and make suggestions as to which policy factors should be implemented in the future. For this, priorities were set for the policy factors for activating sports welfare culture as studied by Kim Min-Kyu and Song Jung-Ran (2018). The key conclusions were as follows. First, for layer 1, it is the policy factors that top priority should be given to. Second, for the sublayer of layer 2, it is service delivery system. Third, for the sublayer of policy factors for layer 2, it is service delivery system. Fourth, for the infrastructure factor, it is content ware infrastructure. Fifth, for the R&D factor of layer 2, it is professionals training system. Sixth, for the related legislative amendment in layer 3 of the policy factors, it is the discussion of supports for related legislative proposals. Seventh, for the policy development factor in layer 3 of the policy factors, it is sports welfare policy project development. Eighth, for improved perception in layer 3 of the policy factors, it is the greater focus on welfare with local governments and related agencies. Ninth, for service delivery system factor in layer 3 of the policy factors, it is the determination of the government ministry and its roles for administrative supports. Tenth, for budget in layer 3 of the policy factors, it is how to get budget. Eleventh, for hardware infrastructure in layer 3 of the infrastructure factors, it is the increase of efficiency in using the existing sports facilities. Twelfth, for human ware infrastructure in layer 3 of the infrastructure factors, it is the recruit of sports welfare instructors. Thirteenth, for content ware infrastructure in layer 3 of the infrastructure factors, it is the development of sports welfare programs. Fourteenth, for the professional training and operation system in layer 3 of the R&D factors, it is the development of a curriculum for sports welfare studies. Fifteenth, for basic data research in layer 3 of the R&D factors, it is the mutual exchange between sports and welfare professionals. Sixteenth, for planning and R&D in layer 3 of the R&D factors, it is the development of alternative programs in sports welfare. The results of the study will be helpful in organically combining the policy course as a whole with the academic disciplines in general.

Effects of Mechanical Stimulation on the Proliferation of Bone Marrow-derived Human Mesenchymal Stem Cells

Choi, Kyung-Min,Seo, Young-Kwon,Yoon, Hee-Hoon,Song, Kye-Yong,Kwon, Soon-Yong,Lee, Hwa-Sung,Park, Jung-Keug Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.6

To support and enhance the in vitro growth and activity of mesenchymal stem cells (MSCs), the cell culture medium may be supplemented with various proteins and factors to mimic the physiological environment in which the cells optimally proliferate and differentiate. In this study, the effects of mechanical factors on cellular metabolic responses were investigated experimentally using a bioreactor. The effects of various chemical factors, such as growth factors, cytokines, and hormones, were also investigated. Based on previous reports demonstrating the important roles of mechanical factors in the growth and activity of MSCs, we sought to evaluate the effects of mechanical stimuli on the proliferation of bone marrow-derived MSCs using a cell training bioreactor that imposed cyclic mechanical stretch, with parameters of 240 min/day, 0.03 Hz, and $5{\sim}15%$ strain. The application of cyclic stretch $(5{\sim}15%\;strain)$ to the MSCs enhanced their proliferation during the early stage(3 days), but not the late stage (14 days), of batch culture. Mechanical stretch did not increase the release of lactate dehydrogenase (LDH) from the MSCs during culture. Appropriate levels of mechanical stretch $(5{\sim}10%\;strain)$ increased collagen synthesis, but did not alter MSC stretch was able to serve as a potent positive modulator of MSC proliferation during the initial stages of culture.

Improving the subcritical water extraction of flavonoids narirutin and hesperidin from Citrus unshiu peel by pulsed electric fields

Hui-Ju Kim,Mi-Ri Kwon,Hye-Ji Kang,Na-Yeon Kim,Hee-Jeong Hwang,Min-Jung Ko,Myong-Soo Chung 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.11

Citrus fruit is important source of flavonoids, mainly flavanones which are narirutin and hesperidin. Those citrus flavonoids have been found to have health-related properties including antioxidant, anticancer, and anti-inflammatory. The main purpose of this study was to verify that the extraction of narirutin and hesperidin from Citrus peel can be more effective by combining pulsed electric field (PEF) pre-treatment and subcritical water extraction (SWE). Citrus unshiu peels were treated with PEF under conditions of electric field strength (3 kV/cm) and times (1 and 2 min). Subsequent SWE was conducted by using a Dionex Accelerated Solvent Extractor (ASE, Model 350) at extraction temperature 170°C for 10 min. The total flavonoids content was measured by using the aluminum chloride colorimetric method and the antioxidant capacity was analyzed by the Ferric reducing antioxidant power (FRAP) assay using spectrophotometer. The concentrations of narirutin and hesperidin were increased as PEF pre-treatment time increased. The highest concentrations of narirutin and hesperidin were 13.41 mg narirutin/g dry citrus peel and 141.16 mg hesperidin/g dry citrus peel at PEF pre-treatment condition of 3 kV/cm and 2 min. The total flavonoids contents of the extracts increased 105.2% and 123.1% for citrus peel PEF treated at 1 and 2 min, respectively. In addition, compared to the untreated sample, PEF pre-treatments of 1 and 2 min increased the antioxidant capacity of the extracts 109.2% and 160.8%, respectively. Therefore, the results demonstrate the potential of PEF pre-treatment to improve the SWE of flavonoids from citrus unshiu peel.

식품중 중금속 규격 과학화를 위한 조사연구(Ⅲ) : 음료수의 중금속 향량에 대하여 Heavy metal contents beverages

김명철,김미혜,권기성,정소영,박성국,이윤동,이승훈,김은정,강민철,박효정 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 연구는 국내에 유통되고 있는 음료류중 중금속 함량을 파악하여 식품공전 제 ·개정시 과학적 기초자료로 활용하고자 수행하였다. 출 2☞7여건의 음료류에 대하여 유도결합플라스마 분광기(ICP), 원자홉광광도 계(AAS) 및 수은분석기 등을 치용하여 납, 카드윰 등 중금속함량을 측정하였으며 그 결과는 다음과 같다 [단위 : min-ma)t(irran) ; mrk3 ; f : 0.0001-0.0025(0.0003), As :불검출곰.OB45(0.0003), Pb : 불검출-0.0893(0.0041),Cd ' 불검출곤.0064(0.0011), Cfl : 불검출.(i9(0.05), Mn : 불검출글.찌(0.16), Zn : 불검출-12.85(0.뽀), Sn :불검출-45.36(1.97). 본 조사결과 우리나라에서 유통되고 있는 음료류 중 납 등 중금속 함량은 외국의 모니터랑 결과와 유사하였다. 또한 우리나라 국민이 음료류를 통한 중금속 섭취량은 FAO/UTfO에서 설정한 잠 정주간섭취처용량의 약 0.OleO.06% 이하로 매우 낮았다. 편 연구조사에서 먼어진 결과는 음료류 중 납 기준 설정을 위한 중요한 기초자료로 활용할 수 있는 것으로 사료된다. This study was conducted to estimate the cantents of heavy netals in beverages and to fIFovide a scientific basis for heav)· meta3 standardizatior of Korea Food Code. The contents of rriercur17(Hg), lead(Pb), cadmium(Cd), arsenic(As), manganese(Mn),ziac(Zn), tin(Sn) and topper(Cu) were determined in a total of 20? samples of beverages usinga mercury anal!'zer, aromic absorption spectrophotometer(AAS) and inductiuety coupled plasmaspectrometer(fcpl. The vatues of heavy metals In beverages were as fellows (min-max (mean) , mg/krl , Hg : 0.DOOI-0.0095(0.0004), As : ND-0.0245(0.0003), Pb ND-0.0893(0.0041), Cd : ND-0.0064(0.0011),Cu : ND-1.69(0.03), Mn : ND-3.94(0.16), Zn ND-12.85(0.28), Sn : ND-45.36(1,97). Our results weresimilar to thofe reported by other countries. It is thought that our results could be utilizedimportant references toT es?ablish the standard of lead in beverages.

크론병에 대한 CoPPLX의 방어효과에 대한 연구

이호학,김유림,문창기,이재일,양병윤,김종수,이정민,정재혁,안석진,박상준,김윤권,김소연,김영중,조민구,최영자,장성조 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1