뇌동맥류 환자의 임상분석

강삼석,박중욱,류재광,신동철,김봉환 全南大醫大 1983 전남의대학술지 Vol.20 No.1

반응표면분석법에 의한 동충하초 첨가 증편 제조의 최적화

박금순,윤광섭,황성희,조현정,김정숙 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.4

To optimize the preparation process of Jeung-Pyun, an experiment was derived, using a central composite design, to find the optimal mixing conditions. The addition of Paecilomyces japonica powder, the volume of Tak-Ju and the second fermentation time were independent variables, with the pH, volume, color, sensory and texture properties of the Jeung-Pyun selected as response variables. As the Paecilomyces japonica powder and fermentation time were increased, the b values and volume after the second fermentation also increased. The color of the Jeung-Pyun became deeper with increases in the Paecilomyces japonica powder and fermentation time, and was deepest on the addition of 30% Tak-Ju. The pH increased with increasing fermentation time of the Jeung-Pyun, and on the addition of 3% Paecilomyces japonica rapidly increased more than with the other groups. The optimal mixing conditions for the best quality Jeung-Pyun, with restricted conditions above a b value of 3, a color intensity above 3, and a volume of 40㎖ and a pH of 4.02~4.04 after the second fermentation, were 4.0%, 20% and 45 mins for the Paecilomyces japonica powder, Tak-Ju and second fermentation time, respectively.

대장암환자에서 자연살해세포의 변화

김광호,심강섭,박응범,강은숙,홍기숙 대한대장항문학회 1998 Annals of Coloproctolgy Vol.14 No.3

Polytertrafluoroethylene의 직경에 따른 신생내피의 조직학적 변화에 대한 실험적 연구

박해일,이광석 고려대학교 의과대학 1998 고려대 의대 잡지 Vol.35 No.2

In synthetic arterial grafts the regeneration and proliferation on the inner surface of graft is a part of the normal healing process. However, there are controversies in the source of these cells and the pattern of proliferation. Many investigators have suggested that endothelium might be derived from arterial sources at the ends of grafts, capillaries growing through the graft matrix from the outside, and endothelial cells can circulate in the blood and settle onto the luminal surface. The purpose of this study was to investigate the patency rates and the histologic findings of neoendothelialization according to the diameter of polytetrafluoroethylene(PTFE) which is the most widely used for synthetic graft. Ten mongrel dogs, weighing 20 to 25kg, were used for this study. Under the operating microscope, all grafts were implanted in both femoral arteries of dogs by interrupted end-to-end microanastomosis. They were divided into two groups according to the diameter of implanted PTFE. Each group comprised five dogs. The lengths of implanted PTFE were 20mm in both groups. All implanted grafts were 25㎛ in fibril length and 0.39mm in wall thickness. The internal diameter of graft in group Ⅰ was 3mm and that of graft in group Ⅱ was 5mm. Two grafts of one dog per group were harvested at 1, 2, 4, 8, and 12 weeks after implantation respectively. All PTFE grafts were observed for patency rates and the histologic findings with light microscope and scanning electron microscope. The results obtained were as follows : 1. The patency rate was higher when the diameter of implanted PTFE was larger than that of recipient vessel, and the patency rate in grafts without infection was higer than that in grafts with infection. 2. Light microscopically, in group Ⅰ, a few cells of neointima is noted at the end of the inner surface of PTFE at 1 week after implantation and the histologic findings could not be observed due to occlusion of grafts at 2, 4, 8, and 12 weeks after implantation. In group Ⅱ, the neointimal lining is advanced across the anastomosis at 1 week after implantation and the histologic findings could not be observed due to occlusion of grafts at 2 and 8 weeks after implantation. At 4 weeks after implantation, neointimal lining was more advanced to the midportion of implanted PTFE. At 12 weeks after implantation, the entire inner surface of implanted PTFE was lined completely by neointima and subintimal tissue. 3. Scanning electron microscopically, in group Ⅰ, the inner surface of implanted PTFE was lined by amorphous fibrin layer at anastomosis site at 1 week after implantationin and the histologic findings could not be observed due to occlusion of grafts at 2, 4, 8, and 12 weeks after implantation. The histologic findings of group Ⅱ were similar to those of group Ⅰ at 1 week after implantation and could not be observed due to occlusion of graft at 2 weeks after implantation. At 4 weeks after implantation, flat layer like normal endothelium was noted at anastomosis site. At 8 weeks after implantation, flat neointima was noted in the midportion of implanted PTFE. However, the inner surface of implanted PTFE was still noted at 12 weeks after implantation. 4. Neoendothelium was noted only in the patent cases with neointimal covering of the inner surface of PTFE. In conclusion, at 1 week after implantation neoendothelium started to grow from both anastomoses and at 8 weeks neoendothelium was formed in the entire inner surface of implanted PTFE. Because PTFE is not elastic like normal vessel, PTFE with larger diameter than that of recipient vessel is recommended as the grafts fot implantation.

다운증아 어머니의 기대성향이 비계화 교수와 문제해결학습에 미치는 효과

여광응,윤숙경,박현옥 한국특수교육문제연구소 2003 특수교육저널 : 이론과 실천 Vol.4 No.1

이 연구는 어머니-다운증아의 상호작용을 통한 문제해결과정에서 어머니의 기대성향이 어머니 교수중재의 양과 수준에 미치는 영향과 다운증아의 적목피라미드 문제해결과제에 미치는 효과를 밝히는데 두고 다운증아 어머니의 기대성향에 따른 어머니의 비계화 교수와 아동의 문제해결학습간의 관계를 탐색하고자 하는 것으로,만 6세인 다운증아 남아 3 명과 여아 3명인 전체 6 명 그리고 그 어머니들을 대상으로 하였다. 어머니-아동의 문제해결상호작용과정을 비디오로 촬영하여,어머니의 교수중재는 중재 수준,중재 양,유관 및 비유관 여부를 중심으로 그리고 아동의 문제해결은 성패를 중삼으로 양적 · 질적 분석을 병행 하였다. 연구결과 어머니의 기대성향이 긍정적일수록 아동의 문제해결 성패에 기반하여 중재 양과 중재 수준을 조절하는 유관 중재를 많이 사용하고 문제해결학습에서 부정적 기대성향의 다운증 자녀보다 더 긍정적 학습결과를 보인다. 또한 어머니의 비계화 교수 변인과 자녀의 문제해갤학습 변인사이에는 상호 관련작 양방향성 효과가 있다. The purpose of this study was to examine the relationships between maternal expectancy and maternal use of scaffolded instruction and problem-solving skills of children with Down syndrome. For this purpose, three boys and three girls with Down syndrome whose age were six years old and their mothers participated in this study. The mother-child interaction regarding problem-solving tasks was videotaped and analyzed. The qualitative and quantitative analyses were conducted to find out the condition and quantity of scaffolded instruction used by mothers, and degree of their contingency of the tasks. In addition, it was analyzed whether these children resolved given problems successfully or not. As a result, it was found that the mothers who had more positive expectancy relied more on contingent intervention that regulated the quality and quantity of instructions based on the success or failure of their child' s problem solving tasks, and that their children produced better learning outcome than the children of the mothers who had more negative expectancy. In addition, two-way interrelationships between the scaffolded instruction variable of the mothers and their children ’ s problem-solving learning variable were found.

성균관대학교 물리학과 방음실의 설계 및 제작

朴興秀,尹錫旺,李聖壽,朴光俊,吳世珍 성균관대학교 기초과학연구소 1986 論文集 Vol.37 No.1

An anechoic chamber was constructed to make to condition of acoustic free field at the Department of Physics, Sung Kyun Kwan University. In this paper the criteria for the design and construction of the anechoic chamber are described. 48cm long wedges of density 15.3kg/㎥ glasswool attached to the 40×20×20㎤ base were used for the lining of anechoic chamber with the cutoff frequency of 125Hz. The interior space of the chamber is 3.56m long, 3.38m wide and 2.37m high.

도재브라켓의 제거방법에 따른 법랑질표면의 주사전자현미경학적 관찰

박미숙,윤영주,김광원 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.5

도재브라켓의 제거방법에 따라 법랑질표면에 미치는 영향을 비교 평가하기 위하여 80개의 발거된 소구치를 대상으로 통법에 의해 도재브라켓을 부착시키고 일주일 후 각각 40개의 기계적 방법에 의한 제거군과 전기열전도 방법에 의한 제거군으로 구분하여 도재브라켓의 제거를 유도한 후, 그 탈락양상 및 도재브라켓과 법랑질 표면의 주사전자현미경 관찰을 시행하였으며, 전기열전도 방법에 의한 제거군을 다시 각각 10개씩 고속 tungsten carbide bur에 의한 연마군, 저속 sof-lex disc에 의한 연마군, 고속 resin polishing bur에 의한 연마군, 그리고 초음파 치속제거기에 의한 연마군으로 분류하여 잔여레진의 연마를 시행한 후, 잔여레진의 연마를 시행한 후, 잔여레진의 평가에 의해 다음과 같은 결론을 얻었다. 1) 결찰와이어 절단용 겸자를 이용한 도재브라켓의 기계적 제거시, 0.69의 평균 잔여레진 부착지수를 보임으로써 법랑질과 레진 경계부에서 파절이 가장 빈발한 양상을 보였다. 2) 전기열전도 방법을 이용한 도재브라켓의 제거시 2.19의 평균 잔여레진 부착지수를 보임으로써 브라켓과 레진 경계부에서의 파절이 가장 빈발한 양상을 보였다. 3) 기계식 방법으로 도재브라켓의 제거시, 법랑질표면의 주사현미경 관찰소견은 실험군의 7.5%에서 법랑질표면의 탈락과 패임 등의 손상을 보였다. 4) 잔여레진의 제거의 고속 resin polishing bur 사용군에서 가장 적은 잔여레진막을 보였다. The purposes of this study were to evaluate and compare the frequency of ceramic bracket fracture, frequency of enamel fracture, bond fracture site, adhesive remnant index after mechanical and electrothermal debracketing, to evaluate effectiveness of high and low speed rotary instrument and ultrasonic instrument during residual adhesive remnants removal, and to measure resin film surface(percentage) using by image analyser(Leco 300). Bond fracture site, bracket fracture, and enamel surface damage were examined by scanning electron microscope. The following results were obtained: 1. In the mechanical debracketing group, the bond failed predominantly at enamel-adhesive interface with the bulk of adhesive remaining on bracket base. 2. In the electrothermal debracketing group, the bond failed predominantly at adhesive-bracket interface with the bulk of adhesive remaining on enamel surface. 3. The most effectiveness of residual resin removal was obtained by means of the resin polishing bur and the order of scratch formation was the precedure using tungsten carbide bur, ultrasonic scaler, sof-lex disc, and polishing bur. 4. The order of the resin film surface percentage was ultrasonic scaler, tungsten carbide bur, sof-lex disc, and resin polishing bur.

圓光醫大 附屬 第2病院 神經精神科 患者에 對한 統計的 考察(Ⅱ)

박민철,장광철,김상원,김한주,유미경,김훈,유용진,김현정,박남진,오상우,이귀행,노승호,백영석 圓光大學校 醫科大學 神經精神科學敎室 1992 圓光精神醫學 Vol.8 No.1

본 연구는 1986년 7월 11일부터 1991년 7월 10일까지 원광의대 부속 제2병원 신경정신과에서 의래 및 입원치료를 받았던 6,072명의 환자를 대상으로 병원 역학조사를 실시하고 그 결과를 1차조사 (박민철 등1986)와 비교했다. 1.전체 환자 중 남자가 54.4%로 여자보다 많았고, 이리와 이리 인접지역이 84.0%로 대부분의 지역을 나타났다. 의료보장상태는 보험환자가 57.1%, 의료보호환자21.8%, 일반환자 21.1%였으며 연령별로는 20대 24.6%, 30대 22.8%로 20대 30대가 절반정도를 차지했다. 2.외래환자에서는 신경중성장애가 46.0%로 가장 많고 정동장애, 경련성장애 순이며, 입원환자에서는 정신분열증이 30.5%로 가장 많고 기질성정신장애, 정동장애의 순이었다. 3.남자는 신경증성쟝애(23.4%), 정신분열증, 기질성정신장애 순이고 여자는 신경증성장애(39.9%), 정동장애, 정신분열증의 순이었다. 남자가 여자보다 많은 진단은 알코올정신장애(94.6%), 기질성정신장애, 소아정신장애 순이고, 여자가 남자보다 많은 진단은 정동장애(64.5%), 신경증성장애였다. 4.진단별 연령분포에서 신경증성장에는 30대(25.3%), 20대, 40대 순이고, 정신분열증은 20대(40.5%), 30대, 40대 순이며, 정동장애는 20대, 30대, 50대 순이나 비교적 비슷한 융이었다. 5.진단별 발병연령에서 신경증성장에는 20대, 30대가 절반이고,정신분열증은 20대(51.3%), 10대, 30대 순이며 정동장애는 20대(30.7%), 30대, 50대 순이었다. 진단별 평균 발병연령은 신경증성장애 32.7세, 정동장애 36.1세, 정신분열증 23.6세였다. 6.진단별 이병기간은 2년이상이 신경증성장에 28.3%, 정신분열증 68.5% 정동장애 43.9%인데 전체적으로 41.0%였다. 진단별 평균 이병기간은 정신분열증 73.2개월, 정동장애 45.5개월, 신경증성장애 29.5개월이었다. We examined epidemiologic study of 6,072 patients who visited Wonkwang Neuropsychiatric hospital since July 11 of 1986 until July 10 of 1991 and compared this results with previous study(Park et al 1986). The study revealed as follows:1. Of total patients males visited this hosptial more than females as 57.1%, Most of them(84.0%) resided Iri and neighbouring Iri. In the point of insurance, insured patients, medicaid patients and uninsured patients were 57.1%, 21.8% and 21.1% respectively. On the distribution of age, the twenties, the thirties were 24.6%, 22.8% respectively and twenties and thirties were about half of total patients. 2. Of outpatients neurotic disorder was the most as 46.0%, secondly mood disorder and thirdly convulsive disorder, but of inpatients schizophrenia was the most as 30.5%, secondly organic mental disorder and thirdly mood disorder. 3. Of males neurotic disorder was the most as 23.4%, secondly schizophrenia, and thirdly organic mental disorder, but of females neurotic disorder was the most as 39.9%, secondly mood disorder and thirdly schizophrenia. alcoholic mental disorder(94.6%), organic mental disorder, child mental disorder were mostly found in males,but mood disorder(64.5%) and neurotic disorder were mostly found in females. 4. On the distribution of age neurotic disorder was found the most in the thirties(25.3%), next in the twenties and in the forties, and schizophrenia was found the most in the twenties(40.5%), next in the thirties and in the forties, but mood disorder was found the most in the twenties, next in the thirties and in the forties but revealed similar rates relatively. 5. On the distribution of age of onset neurotic disorder developed nearly half during the twrnties and the thirties, schizophrenia developed mostly during the twenties (51.3%), secondly the teens and thirdly the thirties, but mood disorder developed firstly the twenties(30.7%), secondly the thirties and thirdly the fifties. on the average age of onset of illness neurotic disorder, mood disorder and schizophrenia were 32.7 years, 36.1 years and 23.6 years respectively. 6. On the duration of illness more than two years was 28.3% in neurotic disorder, 68.5% in schizophrenia and 3.9% in mood disorder and on the average duration of illness schizophrenia, mood disorder and neurotic disorder were 73.2 months, 45.5 months and 29.5 months respectively.

남자 펜싱선수의 연령에 따른 운동반응연구

박인기,우운수,현광석 忠南大學校體育科學硏究所 1997 體育科學硏究誌 Vol.15 No.1

The purpose of this study was to identify the difference between the Whole Body Reaction Time at offensing movement and that at defencing movement among three age groups of male fencing players. The subjects of this study were twenty-eight male fencing players in Taejon City(10 middle school players, 10 high school players and 8 collegiate players). Base upon the results of this study, the following conclusions were obtained. 1. Whole Body Reaction Time at offensing and defencing movement showed fast record in the order of high school group, college group and middle school, but there was no significant difference. 2. Whole Body Reaction Time at offensing movement showed fast record in the order of college group, high school group and middle school. And there was significant difference between college·high school group and middle school group in the comparison of offensing WBRT. 3. Whole Body Reaction Time at defencing movement showed fast record in the order of college group, high school group and middle school. And there was significant difference between college·high school group and middle school group in the comparison of defencing WBRT. 4. WBRT at offensing movement was faster than that of defensing movement. But there was no significant difference between offense movement and defense movement in the comparison of WBRT.

단기배양을 통한 말초혈액 CD34 양성세포의 체외증폭

박상준,김철희,배광봉,김현수,김종숙,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Background: It is suggested that clinical practice in the areas of bone marrow transplantation and gene therapy might rely on the ex vivo expansion of hematopoietic stem/progenitor cells. However, the condition for ex vivo expansion of hematopoietic progenitor cells is not well established. The authors pursued a series of experiments to define the proper conditions for the expansion of hematopoietic cells in the short-term liquid suspension culture of mobilized peripheral blood CD34+ cells. Methods: 1.0ml cultures were initiated with 9×10^3 PB CD34+ cells, which were isolated from PB mononuclear cells (MNCs) by high-gradient cell sorting, in 12 well plates with the various combinations of hematopoietic growth factors(HGF). The following recombinant human HGFs were used: stem cell factor(SCF) 100ng/ml, granulocyte colony-stimulating factor(G-CSF) 100ng/ml, GM-CSF(granulocyte, macrophage colony-stimulating factor) 100ng/ml, interleukin-1 beta(IL-1B) 1ng/ml, interleukin-3(IL-3) 20ng/ml, interleukin-6 (IL-6) 100ng/ml. At the end of culture, colony-forming cells were evaluated by semisolid clonogenic assay. Results: 1) Using the high-gradient magnetic sorting system, CD34^+ cells were isolated with a yield of 40 3% 2) In 7 day culture of PB CD34^+ cells(9×10^3 cells), nucleated cells expanded mean 10×10^3(range, 9 to 20×10^3) with the addition of SCF alone, 35×10^3(range, 10 to 60×10^3) with SCF plus G-CSF plus GM-CSF, and 130×10^3(range, 40 to 300×10^3) with the combination of SCF, G-CSF, IL-1, IL-3, IL-6, GM-CSF. In 14 day culture, nucleated cells expanded 10×10^3 to 1,860×10^3 with combination of human hematopoietic growth factors. 3) In 10 day culture without medium change of PB CD34^+ cells, CFU-GM numbers expanded 16. 5 fold(range, 7 to 59 fold) with the addition of SCF plus G-CSF plus Il-1 plus IL-3, 31.3 fold(range, 20.5 to 101.1 fold) with the combination of SCF, G-CSF, IL-1, IL-3, GM-CSF. In 14 day culture with or without medium change of PB CD34^+ cells was inferior to 10 day culture for CFU-GM expansion. 4) There was no significant difference for CFU-GM expansion between five growth factors(SCF,G-CSF,IL-1,IL-3,GM-CSF) and six growth factors(five growth factors plus IL-6). Conclusion: The authors could confirm that short-term suspension culture of peripheral blood CD34+ cells could expand hematopoietic progenitor cells. Ten-day culture with medium change of CD34+ cells with the addition of five growth factors, i.e. SCF, G-CSF, IL-1B, IL-3, and GM-CSF, might be the most efficient in this system.