Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

트레드밀 운동시 흡기근 테이핑이 폐활량에 미치는 영향

김민지,신수영,송월섭,조수진,최동락,황미진,황진규,박진현,김경,Dennis W. Fell 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 트레드밀 운동과 키네시오 테이핑의 효과에 따른 폐활량의 변화를 알아보기 위해 26명의 비흡연자가 참가하였으며 키네시오 테이핑을 적용하지 않은 그룹과 키네시오 테이핑을 적용한 두 그룹으로 무작위로 배정하고 각 군들을 주 3회 6주간의 트레드밀 훈련을 실시하였다. 실험 전과 후, 스파이로미터를 사용하여 키네시오 테이핑의 적용 따른 폐활량 변화 효과를 측정하였다. 이 연구의 결과를 종합해보면 키네시오 테이핑을 적용 그룹에서 적용하지 않은 그룹에 비해 FVC, FEV1에서 유의한 증가를 보였다. 이러한 결과는 건강한 성인에서 키네시오 테이핑을 적용한 경우 키네시오 테이핑을 적용하지 않고 트레드밀 운동을 한 경우보다 폐활량의 향상에 효과적이라고 생각되어진다. The purpose of this study was to investigate the variation of vital capacity(VC) according to the effects of kinesio taping with treadmill exercise. Twenty-six non-smokers were participated in this research and these subjects were randomly assigned into two groups. To measure the VC variation effects of kinesio taping, spirometer was used. The collected data were analyzed statistically by using a paired Mauchly test and repeated measure ANOVA. The results of this study were as follows; A group: treadmill with kinesio taping B group: treadmill without kinesio taping. Between A and B, there were significant differences. In the case of A group, there was 11.66% increase of VC, during 3 weeks experiment(p<.01). In the case of B group, there were significant differences, 3.35% increase of VC, during 3 weeks taping intervention(p<.01). After 6 weeks experiment, the improvement of VC shown a significant difference with intergroup (p<.05). From this result, it was revealed that treadmill exercise with kinesio taping was effective to improve VC to healthy adult than treadmill exercise without kinesio taping.

免疫黃金 標識法을 이용한 肺吸蟲의 體液 抗原에 관한 硏究

宋坰元,林漢鍾,金洙鎭 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.1

In order to observe the antigenic localization in the tissues of the young adult Paragonimus westermani, immunogoldlabeling method was applied using serum immunoglobulins (IgG) of rabbit immunized with antigen which was obtained from the body fluid of the adult worm. The sectioned worm tissue was embedded in Lowicryl HM 20 medium and stained with serum IgG of immunized rabbit and protein A gold complex(particle size : 12nm). It was observed by electron microscopy at each tissues of the worm. The gold particles were observed on the tegumental syncytium as well as cytoplasm of the tegumental cell, basal lamina of the tegument and interstitial matrix of the parenchyma. But the gold particles were not observed on the muscle system and mitochondria of the tegument. The gold particles were veFy specifically labeled in the secretory granules in the vitelline cells. They were also predominantly labelled on the caecum epithelial syncytium and interstitial matrix of caecal epithelium than epithelial lamela and lumen of caecum. The above findings showed that antigenic materials of body fluid of the worm specifically concentrated on the cytoplasmic matrix of the tegumental cell, basal layer matrix and secretory granules of the vitelline gland cells.

반수염석고 첨가 이산칼슘 복합 시멘트의 성질

송 진,김광만,김경남 대한치과기재학회 1996 대한치과재료학회지 Vol.23 No.1

The calcium phosphate cement(CP) and calcium sulfate hemihydrate(CS) composite cements, of which the weight % of CS were 25(CP75), 50(CP50), 75(CP25), 100(CS), were prepared and mixed with either distilled water(S solution) or sodium hydrogen phosphate solution(P solution). The setting times, temperature rises, compressive strengths, microstructures of scanning electron microscopy(SEM) view and powder X-ray diffraction(XRD) patterns of the cements were studied. The results were as follows: 1. When the composite cements mixed with S solution, the more the CS content was, the shorter the setting time and the higher the compressive strength were(p<0.05). 2. When the composite cements mixed with P solution, the more the CS content was, the longer the setting time and the lower the compressive strength were, and they showed significantly inferior physical and mechanical properties compared with the groups using S solution(p<0.05). 3. The temperature rise was about 1∼2℃ in the composite cement groups. 4. SEM photographs showed that the fully set composite cements consisted of large gypsum crystals mixed with small rod-like hydroxyapatite crystals. 5. XRD revealed that CP was mostly converted to hydroxyapatite and CS was to calcium sulfate dihydrate after reaction, respectively. The conclusion was that the composite cements mixed with S solution had superior physical and mechanical properties to the CP alone and especially the CP75-S group showed the best physical and mechanical properties in this study, so it was the CP75-S group that had the potential to be used as periodontal bone graft materials.

광조사시간에 따른 광중합형 치주팩의 물리적 성질

김경남,최용석,송진 大韓齒科器材學會 1995 대한치과재료학회지 Vol.22 No.2

Periodontal dressing materials have been used for patients' comfort, wound protection and fixation of teeth for 70 years. Recent1y light-cured type periodontal dressing material, main component of which is polyether polyol, was introduced and is easy to control the setting time and esthetic with translucent pink shade. But there are potential of toxicity and decreasing of physical properties resulting from incomplete light curing. There have been many studies on toxicity but a few on physical properties. Material used in this study was ??(Caulk, U.S.A.) and specimen of 1 ×1 ×10mm was light cured at both ends and center to be stored at 37℃, 100% relative humidity for 30min. The curing time was 10, 20, and 30sec. The physical properties such as tensile strength, elongation and elastic modulus were measured using universal testing machine(Instron 6022, U.K.) at the cross-head speed of 10mm/min. The data were analysed using ANOVA. Results were as followers: 1. The tensile strength of 10 sec. group was significantly, lower than others(p<0.05). 2. There was no significant difference in elongation among all groups(p>0.05). 3. For elastic modulus at 1% elongation, 10 sec. group was significantly lower than others(p<0.05). 4. For elastic modulus at 5% elongation, each group was significantly different from others(p<0.05). 5. The elastic moduli at 5% elongation of 10 sec. group and 30 sec. group were significantly higher than those at 1% respectively. It was concluded that 20 sec. light cure would be effective in clinical use considering that the tensile strength and elastic modulus of this group were higher than those of 10 sec.

Real-Time IR을 이용한 Polyesterimide의 경화거동 연구

홍진후,송주명,김현경,박미영,박기주 조선대학교 생산기술연구소 1999 生産技術硏究 Vol.21 No.2

Polyesterimide for enamel Wire coating was synthesized and its curing reaction was monitored by real-time FT-IR. Infrared 1019cm^(-1) band was used to monitor the curing process. Results of in-situ analysis showed that evaporation of ethylene glycol and transesterification started from 100℃. When curing temperature was higher than 200℃, ester reaction was favored over the ether reaction. It was concluded that curing temperature is the key factor to control the thermal stability of the enamel wire coating.

분화된 갑상선암 환자에서 수술 후 재발과 원격전이를 예측하는 혈청 갑상선글로불린치

김경수,류진숙,홍석준,김원배,송영기 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

연구배경 분화된 갑상선암의 재발이나 원격전이를 시사하는 혈청 갑상선글로불린치는 보고자들마다 차이가 있고, receive operating characteristics (ROC) 곡선을 이용해서 cut-off value을 구한 경우는 별로 없다. 또한 수술 후 방사성요오드 치료 직전 (Tg-RAI), T₄투여 도중(Tg-on) 및 T₄ 중단 후(Tg-off)에 측정한 갑상선글로불린의 임상적 의의에 대해서도 아직 이견이 있다. 더구나 임상에서 측정된 혈청 갑상선글로불린치의 의미를 해석할 때 보다 큰 의미를 가지는 양성 예측치와 음성 예측치를 조사한 보고는 거의 없다. 방법: 갑상선 유두암 또는 여포암으로 진단되어 갑상선 전절제술 및 고용량 방사성요오드 치료를 시행 받은 후 갑상선 자리에 잔여병소 없이 추적관찰이 이루어지고 있는 242명의 환자들 중, 항갑상선글로불린항체가 음성인 205명의 환자를 대상으로 후향적으로조사하였다. 평균 추I:기간은 5년이었다. Tg-off는 저요오드식이를 2주 이상 시행한 후 ^(131)I-전신스캔과 함께 측정되었으며, 내인성 TSH의 농도가 30 μU/mL 이상으로 상승했을 때 의미 있는 것으로 판정하였다. 재발은 경부 림프절을 포함하여 경부에 새로운 병소가 생겼을 때로, 원격전이는 종격동 림프절을 포함하여 경부 이외의 장기에 병소가 생겼을 때로 정의하였곤, 재발이나 원격전이는 각종 영상학적 또는 병리학적 검사에서 이상소견이 발견되는 경우 있는 것으로 판단하였다. 민감도와 특이도가 가장 높은 Tg의 cut-off value를 구하기 위해 ROC 곡선을 이용하였고, cut-off value에 따른 양성 예측치와 음성 예측치를 구하기 위해 2×2표를 이용하였다. 결과: 재발은 50명 (24.4%)의 환자에서 발생하였고, 원격전이는 17명(8.3%)의 환자에서 발생하였다. 재발이나 원격전이 중 어느 한 가지라도 있는 환자는 52명(25.4%)이었다. ROC 곡선 아래부분의 면적을 통해 알아본 Tg-RAI, Tg-on, Tg-off 검사의 정확도는 서로 비슷하게 높았다. 재발에 대한 혈청 갑상선글로불린치의 cut-off value는 Tg-RAl가 11.8, Tg-on이 1.4,Tg-off가 3.3ng/mL였다. 이때의 민감도는 각각 85.4,82.2, 93.3%였고, 특이도는 각각 89.2, 92.4, 88.0%였으며, 양성 예측치는 각각 71.9, 77.1, 70.0%였고, 음성 예측치는 95.0, 94.4, 97.8%였다. 원격전이에 대한혈청 갑상선글로불린치의 cut-off value는 Tg-RAl가27.4, Tg-on이 2.5, Tg-off가 7.9 ng/mL였다. 이때의민감도는 각각 86.7, 87.5, 92.3%였고, 특이도는 각각86.2, 90.8, 80.2%였으며, 양성 예측치는 각각 34.2,46.7, 25.0%였고, 음성 예측치는 각각 98.7, 98.8,99.3% 였다. 결론: 혈청 갑상선글로불린치는 분화된 갑상선암의 재발이나 원격전이를 예측하는 데 있어 민감도와 특이도가 비교적 높은 검사이나, 음성 예측치가 높은 것에 비해 양성 예측치가 낮은 단점이 있었다. 또한 Tg-on은 Tg-off에 비해 양성 예측치는 높았으나, 음성 예측치는 낮게 나타났다. 따라서 Tg-on이 cut-off value보다 높게 측정되는 경우에는 반드시 전신스캔 등 재발이나 원격전이 여부에 대한 추가적인 검사를 시행하고, Tg-on이 cut-off value보다 낮게 측정되더라도 가능하면 선별검사로서 Tg-off나 recombinant human TSH 자극 후의 혈청 갑상선글로불린치를 주기적으로 측정해야 할 것으로 사료된다. Background: Reports on serum thyroglobulin(Tg) levels being used to predict recurrence or distant metastasis during the follow-up of patients with differentiated thyrod cancer(DTC) has been inconsistent. In addition, there have been few reports that attempt to define the cut-off value of Tg for recurrence or distant metastasis obtained by a receiver operating characteristic (ROC) curve. As well, there are differences in opinions on what the value should be on the first serum Tg level measured just before radioactive iodine(RAI) ablation(Tg-RAI), during thyroxine administration (Tg-on), and after thyroxine withdrawal (Tg-off) during the follow-up. Reports on the positive predictive values(PPVs) and negative predictive values(NPYs) of these Tg values are rare. Methods: A total of 205 patients (42 males, 163 females) with DTC were studied. All patients had undergone total or near-total thyroidectomy. After surgery and RAI ablation, annual thyroxine withdrawal 131I-whole body scan (WBS) with Tg measurements was performed. The mean duration of follow-up was 5.0 (1.4~7.4) years. The most sensitive and specific Tg values)cut-off values) for tumor recurrence and/or distant metastasis were selected by using ROC curves. We also calculated the PPVs and NPVs for rcurrence and/or distant metastasis using two-by-two tables. Results: Cut-off values of Tg-RAI, Tg-on, and Tg-off for recurrence were 11.8, 1.4, and 3.3 ng/mL, respectively. For these values, the sensitivities were 85.4, 82.2, and 93.3%, with the specificities at 89.2, 92.4, and 88.0%. PPVs were 71.9, 77.1, and 77.0% while NPVs were 95.0, 94,4, and 97.8%. The cut-off values for distant metastasis were 27.4, 2.5, and 7.9ng/mL, respectively. For these cut-off values, the sensitivities were 86.7, 87.5, and 92.3%, with the specificities being 86.2, 90.8, and 80.2%. PPVs were 34.2, 46.7, and 25.0% and NPVs were 98.7, 98.8, and 99.3%. Conclusion: All three serum Tg levels were sensitive and specific markers for recurrence and distant metastasis. Their PPVs were low in contrast to the high NPVs. In comparison with Tg-on, Tg-off showed higher sensitivity and NPV as well as lower specificity and PPV. Therefore, in the case of higher Tg-on during the follow-up period, efforts to find recurrence and distant metastasis,such as 131I-WBS, should be done. In addition, regular measurement of Tg-off or Tg after stimulation with recombinant human TSH is recommended as a screening test (J Kor SOC Endocrinol 18:153-165, 2003).

폐인산석고를 혼입한 시멘트 모르타르의 내산성

안양진,윤성진,문경주,송훈,소양섭 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

The purpose of this study is to evaluate the acid resistance of cement mortar admixed with waste phosphogypsum by steam curing admixture. Waste Phosphogypsum is a by-product from the phosphoric acid process for manufacturing fertilizers. It consists mainly of CaSO₄·2H₂O and contains some impurities such as P₂O_5, F^(-) and organic substances. The waste phosphogypsums is made use of 4 forms(Dihydrate, β-Hemihydrate, Ⅲ-Anhydrite and Ⅱ-Anhydrite) which were changed to in low temperature of calcination. The mass change and reduction ratio of compressive strength of cement mortar are investigated to evaluate the acid resistance. The specimens of cement mortar were immersed in HCI(5%) and H₂SO₄(5%, 10%) during 14, 28 and 56days. As a result, mass change and reduction ratio of compressive strength of cement mortar admixed with waste phosphogypsum are lower than cement mortar contained OPC alone. Also, Ⅲ-Anhydrite is comparable to Ⅱ-Anhydrite in acid resistance. Therefore, Ⅲ-Anhydrite phosphogypsum calcined at lower temperature could be used as steam curing admixture for concrete 2th production.

비장애 아동과 전반적 발달장애 아동에서 회전 후 안구진탕 기간의 비교

김진미,송지원,홍은경,김성희,김경미 대한감각통합치료학회 2005 대한감각통합치료학회지 Vol.3 No.1

Objective : The purpose of this study was to get the mean of duration of post-rotatory nystagmus test in normal children and to differentiate the duration of post-rotatory nystagmus test between normal children and children with pervasive developmental disorder. Method : 84 subjects were between 3 and 5 years of age and consisted of 64 normal children and 20 children with the pervasive developmental disorder. Analysis of the data was done by using t-test and ANOVA. Results : The results were as follows: 1. Range of duration of post-rotatory nyatagmus test in normal children was 5-22second on left and 7-21 second on right and the mean was 12.63 second on left and 12.59 second on right. 2. Range of duration of post-rotatory nystagmus test in children with the pervasive developmental disorder was 3-11 second on both and the mean was 5.65 second on left and 5.60 second on right. 3. There was no significant difference between males and females with normal children in duration of post-rotatory nystagmus test. However, there was a significant difference of the mean duration between 3 and 5 years old normal group. 4. Children with pervasive developmental disorder significantly have relatively lower duration than the duration of post-rotatory nystagmus test of normal children. Conclusions : The results of the study showed significant difference between normal children and children with pervasive developmental disorder in duration of post-rotatory nystagmus test and suggest that they could be applied to the baseline of clinical therapy.

진단 기준에 따른 취학전 어린이의 중증 유아기 우식증 유병률 비교

임경욱,이광희,라지영,이동진,안소연,김지영,송지현,김윤희 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

연구목적은 중증 유아기 우식증에 대한 두 개의 정의를 진단 기준으로 하였을 때 각 기준에 따른 유병률이 일치하는 정도를알기 위함이었다. 연구대상은 익산시 어린이집 중에서 무작위로 추출한 7개 어린이집의 36개월 이상 71개월 이하 어린이401명 이었다. 치경과 탐침을 사용한 구강검사를 통하여 치아별 우식경험유치면 (dmfs)의 수를 조사하였으며 . 중증 유아기 우식증의 진단 기준은 (1) 상악 전치에 1개 이상의, 와동이 형성된 우식,상실(우식증에 의한), 충전 평활면이 있거나 (2) 우식,상실,충전 치면의 수가 3세는 4개 이상.4세는 5개 이상.5세는 6개 이상인 경우로 하였다. 상악 전치부 우식증에 의한 우식유병률과 우식경험유치면수(dmfs)에 의한 우식유병률은 3세에서 각각 26.7%. 28.9%, 4세에서 각각 32.4%, 30.4%, 5세에서 각각 39.4%, 41.3%, 전체에서 각각 35.4%, 35.9%으로서. 연령별로는 약간의 차이가 있었으나 전체에서는 차이가0.5%로서 거의 일치하였다. 따라서 중증 유아기 우식증의 진단은 두 가지 기준 중에서 어느 하나를 사용하더라도 비슷한 결과가 나을 것이라는 것을 확인할 수 있었다. The purpose of study was to compare the prevalences of severe early childhood cahes(S-ECC) using two different diagnostic criteria. The subjects of this study included 401 preschool children from 36 to 71 months of the randomly selected seven nurseries in Iksan city. Severe early childhood caries was defined as (1) 1 or more cavitated, missing (due to caries), or filled smooth surfaces in primary maxillary anterior teeth or (2) a decayed, missing, or filled score of ≥4 (age 3), ≥5 (age 4), or ≥6 (age 5) surfaces. The prevalence determined by maxillary anterior caries and the prevalence determined by dmfs were 26.7%, 28.9% in 3 years, 32.4%, 30.4% in 4years. 39.4%, 41.3% in 5 years, and 35.4%, 35.9% in all, respectively. The results suggest that the two diag-nostic criteria of the severe early childhood caries yield almost the same results.