자궁암세포(HeLa)에 대한 봉출과 항암제의 병용 효과

서정숙 기전여자대학 1995 論文集 Vol.15 No.-

The purpose of this research was to investigate effect of ethyl alcohol soluble fraction in water extract of Curcuma zedoaria Roscoe(CZE) on the proliferation of human cervical carcinoma cell, HeLa cells. The effects of CZE on the proliferation of HeLa cells, Balb/c 3T3 cells, mouse spleen cells and human lymphocytes were estimated by MTT colorimetric assay. CZE inhibited the proliferation of HeLa cells and Balb/c 3T3 cells. The cytotoxicity of mitomycin C and cisplatin on HeLa cells were increased by the combination of CZE. CZE did not affect on the proliferation of mouse spleen cells and human lymphocytes. These results suggest that CZE inhibit the growth of HeLa cells without the cytotoxicity on mouse spleen cells and human lymphocytes, and increase the cytotoxicity of mitomycin C and cisplatin by the combination of CZE.

녹용 에탄올 분획이 L1210 세포의 Apoptosis에 미치는 영향

서정숙 기전여자대학 2000 論文集 Vol.20 No.-

The administration of 70% ethyl alcohol fraction of Cervus Nippon (CN-E) enhanced the proliferation of thymocytes and the population of CD4^(+)CD8^(-) single-positive cells and the production of interferone gamma in thymocytes and splenocytes. CN-E enhanced the phagocytosis in peritoneal macrophages and suppressed hyphal growth in Candida albicans. To determine whether apoptosis of L1210 cells is due to the direct effect of CN-E or due to the indirect effect through induction of certain soluble factors, 1 tested the cell viability of tumor cells and apoptosis of L1210 cells treated with conditioned medium of CN-E.CN-E did not inhibit the proliferation of tumor cells. Conditioned medium (CM) from cultures of macrophages treated with CN-E induced apoptosis of L1210 cells in vitro and in vivo. CN-E did not enhance the production of NO in peritoneal macrophages. These results indicate that apoptosis of L1210 cells is due to the indirect effect through induction of certain soluble factors from cultures of macrophages treated with CN-E.

산후조리원의 산모 및 아동관리에 관한 연구

서영숙,허정경 한국영유아보육학회 2002 한국영유아보육학 Vol.0 No.29

In study, the purpose is how to manage the service for mother and baby what are Mother's needs and quality degree in the Post-partum Care Center. other purpose is social surporting to be intinate with home caring. For this we investigated the managers and staff in 84 places of the Post-partum Care Center in Seoul and interviewed mothers in 34 place of. It started from in september tenth 2001 until after 3 Weeks. In outcomes, We know careing baby in the Post-partum Care Center is less importent degree. Because, it is less thoughtful of them to care the baby and they are lacked for knowledge about concerning information. In mother-focus care center, it is most important to abserve mother's condition regularly in provided srevice context in the Post-partum Care Center. They are more concerned feeding milk in managing baby, so they use both mother's milk and powered milk. Mother believe that the sanitation check is most important. They requre to be educated about feeding milk and infecting for baby. Finally, we have to provide the professional Materity Manager and establish a low for the Post-partum Care Center's facilities and solve the problem caring the baby being readapted process. We find the way integated and systematic.

채소류중 미량원소의 함량에 관하여

서정숙 忠州工業專門大學 1977 忠州工業專門大學 論文集 Vol.10 No.1

Author investigated the analyse trace elements in vegetables and ginseng which indicated as follows: 1. Gerlio, ginger and onion has considerably large amounts of magnesium, garlic has in proper sequence as manganese, copper, aluminum, bromine and meroury. Ginger has in proper sequence as aluminum, manganese, copper, bomine and mercury, and onion has in proper sequence as copper, manganese, aluminum, bromine and mercury. 2. Carrot has considerably large amounts of magnesium and has in proper sequence as copper, manganese, aluminum, bomine and mercury. 3. Ginseng also has large amounts of magnesium and has in proper sequence as copper, manganese, aluminum, bomine and mercury.

채소류중 미량원소의 함량에 관하여

徐正淑 忠州大學校 1977 한국교통대학교 논문집 Vol.10 No.2

검수 수정 Author investigated the analyse trace elements in vegetables and ginseng which indicated as follows: 1. Garlic, ginger and onion has considerably large amounts of magnesium, garlic has in proper sequence as manganese, copper, aluminum, bromine and mercury. Ginger has in proper sequence as aluminum, manganese, copper, bromine and mercury, and onion has in proper sequence as copper, manganese, aluminum, bromine and mercury. 2. Carrot has considerably large amount of magnesium and has in proper sequnce as copper, manganese, aluminum, bromine and mercury. 3. Ginseng also has large amounts of magnesium and has in proper sequence as manganese, aluminum, copper, bromine and mercury.

단백질 급원과 수준을 달리한 식이가 흰쥐의 납축적에 미치는 영향

김옥경,서정숙,이명환 韓國營養學會 1986 Journal of Nutrition and Health Vol.19 No.4

In this experiment forty-eight Sprague Dawley male rats were chosen and used in order to measure the growth rates and to see the effects of lead accumulation in their organs resulting from variously controlled lead protein diet. Protein sources were casein and isolated soyprotein(ISP), and each source was divided into three groups:7% low protein(LP), 20% standard protein(SP)and 40% high protein(HP) groups. The six experimental groups were given lead acetate(25mg/kg B.W.) and six control groups were given sodium chloride by oral administration 6 times a week for weeks. The results from this experiment were summarized as following; 1) Food consumption, weight gain, oragan weight and food efficiency: Lead acetate administration with protein source had no effects on food consumption, weight gain and organ weight. By their different levels of protein, food consumption of LP group was less than that of SP and HP groups after 3weeks, weight gain of LP group was less than that of SP and HP groups after 1week. The organ weight in LP group was significantly lower than SP and HP groups except teeth and adrenals. Effect of lead acetate administration on food efficiency have significan1ly lower in LP-ISP diet and HP-casein diet than other groups only first week. By their different levels LP group showed significantly lower than SP group until 3weeks. 2) Hematopoietic effect: The hematopoietic effect was not influenced by lead acetate administration and protein source. But the LP group showed a significantly lower hematopoietic effect than the SP, HP groups. 3) Accumulation of lead in the liver, kidney, teeth by protein source showed no significant differences. Accumulation of lead in blood, heart' of LP group, spleen of LP and HP groups, femur of SP and HP groups fed with casein diet groups were significantly higher than fed with ISP diet groups. By their different levels LP group showed generally higher than SP and HP groups. But accumulation of lead in teeth of HP group was high also.

放射線 照射大豆의 醱酵에 의한 成分變化에 關한 硏究

허윤행,이상건,서정숙 서울保健大學 1985 論文集 Vol.5 No.1

Water glass로부터 ZSM-5의 합성

우훈희,홍지숙,서정권,이관영,이정민 한국공업화학회 2002 응용화학 Vol.6 No.2

In order to seek optimum conditions for ZSM-5 formation in the absence of organic template,the effects of both starting composition and water glass as silica source were examined. Water glass was most inexpensive among silica sources, but this was difficulty in reactant control because of contained much alkali ingredient, Considering environmental problem and economic performance,chose alkali drawing-out method to use methanol newly to remove alkali ingredient of waterglass. Characterization of ZSM-5 was carried out by means of XRD, SEM.

골수, 제대혈 및 가동화된 말초혈액에서 Megakaryocyte 집락의 형성능력 비교

홍대식,김정아,김숙자,박성규,서원석,박희숙 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.2

연구배경 : MK 집락배양은 적절한 배양조건과 배양후 MK 집락을 확인하기 어려워 다른 집락배양에 비하여 늦게 시작하였다. 하지만 이러한 문제들이 해결되면서 최근에는 체외에서 MK를 증폭하거나 TPO, IL-11등과 같은 사이토카인의 사용으로 이식 후 혈소판의 감소를 극복하려는 시도들이 활발하게 진행되고 있다. 저자들은 MegaCult^(TM)(Stem Cell Technologies Inc, Vancouver, Canada)를 이용하여 제대혈, 가동화된 말초혈액, 골수를 이용하여, 조혈원 간의 MK 집락 형성능력을 비교하였으며, 앞으로 이러한 방법을 생체 외 MK 증폭 실험 뿐만 아니라 혈소판질환의 병태생리를 연구하는 기초적인 자료로 사용하고자 한다. 방법 : 말초 조혈모세포는 정상인에게 G-CSF 300㎍을 5일간 피하 주사 한 후 혈액분반기를 이용하여 채취하였다. 골수는 동종 골수이식 시 공여자로부터 동의를 얻은 후 채취하였으며, 제대혈액은 정상 분만 후 태반과 제대로부터 얻었다. 단핵구와 CD34^(+) 세포를 분리하여 MegaCult^(TM)를 이용하여 MK 집락을 배양하고, 항 GPIIb/IIIa를 이용하여 이를 확인하고 도립현미경하에서 집락수를 관찰하였다. 결과 : 1) 단핵구를 이용한 MK 집락 비교에서 순수 MK, 혼합 MK 집락 모두 제대혈에서 가장 많은 집락 형성능을 보였고, 골수, 말초 조혈모세포 순이었다. 2) CD34^(+) 세포를 이용한 MK 집락은 각 조혈원에서 양호하게 형성됨이 관찰되었고, 단핵구를 이용한 MK 집락 배양에서처럼 제대혈, 골수, 말초혈액 순으로 제대혈액내에서 가장 많은 집락 형성을 보였다. 3) 단핵구 또는 CD34^(+) 세포를 이용한 집락 배양 시에도 MK를 포함한 전체 집락이 제대혈액에서 가장 많이 형성되었다. 결론 : 각 조혈원의 MK배양에 IL-3, IL-6, TPO가 포함된 MegaCult^(TM)를 사용하였으며 배양된 집락중 megakaryocyte를 확인하기 위하여 항GPIIb/IⅡa 항체를 이용한 면역 조직학적 염색을 시행하였다. 저자들이 시행한 MK집락 배양과 GPIIb/IⅡa를 이용한 면역조직학적 염색은 혈소판의 병태 생리를 이해하고, MK 증폭을 포함한 이식 후 혈소판 회복을 증진시키기 위한 여러 가지 실험의 기초적인 자료가 될 것이다. Background: In vitro colony assay procedures for detecting human erythroid and granulocyte-macrophage progenitors have been in widespread use for many years. However, the development of reproducible assay methods for human megakaryocyte (MK) progenitors lagged considerably behind. There are two main reasons for this: First, the growth conditions initially optimized for other blood cell lineages are not optimal for MK progenitor cell growth. Secondly, the specific recognition of MK colonies requires fixation of the cultures and immunocytochemical staining of the cells for MK-specific antigens. The Methocult^(TM) system for detcting human clonogenic MK progenitors uses a medium containing a defined serum substitude to minimize the inhibitory effects of factors like TGF-β that are found in most sera. Three categories of colonies can be identified in cultures set up with Methocult^(TM): pure MK colonies; mixed MK colonies (containing other lineages as well as MK); and non-MK colonies (containing only other lineages of cells). Methods: This assay has been used to determine the range of MK progenitors present in a series of umbilical cord blood, peripheral blood and bone marrow samples. The MK colonies are detected by staining of the cells with a primary antibody to the MK-specific antigen GPIIb/IIIa (CD41). Results: We enumerated pure CFU-MKs in G-CSF mobilized peripheral blood (MPB), BM and umbilical cord blood (UCB). CFU-MK were detected in MPB, BM, and UCB at incidences of 1.00±0.82, 6.75±2.22, 8.00±1.83 of 5×10⁴mononuclear cells and at incidences of 19.00±03.37, 40.33±17.21, 99.00±54.76 of 5×10³ CD34^(+) cells. The highest number of pure CFU-MK was seen in UCB. The other colony counts (mixed CFU-MK, non-MK CFU) were also higher in UCB than in MPB and BM. Conclusion: We speculate that UCB should be better source of CFU-MK than any other stem cell sources.

청국장 발효중 당성분 변화에 관한 연구

이상건,허윤행,서정숙 서울保健大學 1984 論文集 Vol.4 No.1

Bacillus natto에 의한 대두의 발효시간에 따른 당성분의 함량 변화는 다음과 같다. 1. 증자 대두의 주요 당은 stachyose (4.27%), raffinose (1.30%), maltose (0.56%), galactose(2.38%) 와 glucose (0.18%)이였으며 stachyose와 galactose 함량이 가장 높았다. 2. 0~24시간 발효에서 stachyose 함량이 현저하게 증가하였고, rhamnose & xylose, f-ructose와 ribose는 비로소 생성 되었으며, maltose와 galactose 함량은 계속적인 감소 현상이었다. 3. 증자 대두의 당보다 24시간 발효후의 당이 증가하였고 48시간 발효에서 전체적인 감소현상 이었으며 72시간 후의 당함량은 증가하는 경향이었다. 4. 발효 48~60 시간에서 출국이 적합하였다. Fermentation was carried out on steamed soybeans with a selected Bacillus that is Bacillus natto distributed from KFCC, while change of sugar content was follows: 1. Weight of sugars in steamed soybean were stachyose(4.27%), raffinose (1.30%), maltose (0.56%), galactose (2.38%) and glucose (0.18%), the highest ammount of sugars were stachyose and galactose. 2. During the fermentation 0-24hours, stachyose content increased of over that steamed soybean, while rhamnose xylose, fructose and ribose for the first time were formed and amounts of maltose and galactose had bean decreased continuosly. 3. Sugars contents almost increased after fermented 24hours rather than those contents of steamed soybeans, had bean increased, but after formented 48hours generally had bean disappeared and those contents with 72hours had been increased again. 4. According to fermentation during 48-60hours, conclusion of Chung kuk-jang koji was appropriate.