http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : ( Je Duck Suh ) (검색결과 23 건)
- 무료
- 기관 내 무료
- 유료
-
서제덕(Je-Duck Suh),명훈(Hoon Myung),강나라(Nara Kang),정필훈(Pill-Hoon Choung) 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.5
Purpose: To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression in MG-63 cells and then to find the mechanism b which this regulation occurs. Materials and methods: MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea (76.1 ㎍/㎖), actinomycin D (2.5 ㎍/㎖), cycloheximide (10 ㎍/㎖) were added 1 hour after treatment of 10 nM IGF-I. Results: 1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time- and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide. Conclusion: IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling.
-
-
서경덕(Suh, Kyung-Duck),김문기(Kim, Mun-Ki),천제호(Chun, Je-Ho) 한국해안해양공학회 2011 한국해안해양공학회 논문집 Vol.23 No.4
방파제 등 항만 외곽시설의 설계에 있어서 파랑은 구조물의 단면을 결정하는 가장 중요한 요소이다. 본 연구에서는 Holland(1980)의 파라미터 모델을 이용하여 태풍시의 해상풍을 구축하고, WAM 모형을 이용하여 태풍에 의한 파랑을 수치 계산하였다. 남해안의 부산, 목포, 여수, 서귀포 지역에 영향을 미친 태풍을 선정하고 가상의 태풍을 추가하여 training set을 구성하고, 남향 계열의 7개 파향에 대해서 경험모의기법을 적용하여 재현기간별 설계 파고를 산정하였다. 또한, 이를 POT 방법과 한국해양연구원(2005)의 결과와 비교, 분석하였다. Estimation of wave height is the most important factor in the design of coastal structures such as breakwaters. In the present study, typhoon wind distribution was constructed by applying the parametric model of Holland (1980), and numerical simulations on the typhoon-generated waves were carried out using the WAM. The typhoons which affected the southern coast of the Korean Peninsula and several hypothetical typhoons were selected to construct the training sets. Design wave heights were estimated using the empirical simulation technique for various return periods and wave directions. The estimated design wave heights were compared with those by the peaks-over-threshold method and the results of KORDI(2005).
-
수치 민감도 해석을 통한 파랑중 FPSO운동 시뮬레이션
김제인(Je-in Kim),박일룡(Il-Ryong Park),서성부(Sung-Bu Suh),강용덕(Yong-Duck Kang),홍사영(Sa-Young Hong),남보우(Bo-Woo Nam) 한국해양공학회 2018 韓國海洋工學會誌 Vol.32 No.3
This paper presents a numerical sensitivity analysis for the simulation of the motion performance of an offshore structure in waves using computational fluid dynamics (CFD). Starting with 2D wave simulations with varying numerical parameters such as grid spacing and CFL value, proper numerical conditions were found for accurate wave propagation that avoids numerical diffusion problems. These results were mapped on 2D error distributions of wave amplitude and wave length against the numbers of grids per wave length and per wave height under a given CFL condition. Finally, the 2D numerical sensitivity result was validated through CFD simulation of the motion of a FPSO in waves showing good accuracy in motion RAOs compared with existing potential flow solutions.
-
-
천제호(Chun, Je-Ho),안경모(Ahn, Kyung-Mo),서경덕(Suh, Kyung-Duck),윤종태(Yoon, Jong-Tae) 한국해안해양공학회 2011 한국해안해양공학회 논문집 Vol.23 No.2
본 연구에서는 천 등(2009)의 동적결합형 POM-WAM 모형을 천해역 해빈류 현상 중 하나인 해향저류(undertow) 문제에 적용하였다. 수치모형의 해향저류 계산 결과를 향상시키기 위해 surface roller에 대한 방정식을 풀고, 이를 해향저류 계산에 포함시켰다. 수치모형을 Okayasu and Katayama(1992) 및 Cox and Kobayashi(1997)의 수리모형 실험에 적용하고, 계산 결과를 수리모형 실험 결과 및 Tajima and Madsen(2006)의 수치계산 결과와 함께 비교하였다. 그 결과, 본 수치모형의 계산결과와 실험결과가 잘 일치하는 것으로 나타났다. 이를 통해, 본 수치모형이 쇄파대를 포함한 천해역에 적용 가능함을 확인하였다. In the present study, the dynamically coupled POM-WAM of Chun et al.(2009) was applied to the numerical simulation of undertow, one of the nearshore currents. To improve the accuracy of the numerical model results in surf zone, the transport equation of the surface roller was solved, and its effects were incorporated into the present numerical model. The numerical model has been applied to two hydraulic experiments of Okayasu and Katayama(1992) and Cox and Kobayashi(1997). The numerical results were compared with the hydraulic experimental results to give a good concurrence. It is concluded that the present numerical model can be applied to the shallow water region including surf zone.
-
-
Han, Sang-Ik,Suh, Deuk-Yong,Ha, Tae-Joung,Lee, Myong-Hee,Seo, Woo-Duck,Oh, Seong-Hwan,Jang, Ki-Chang,Lee, Je-Bong,Park, Keum-Yong,Kang, Hang-Won 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4
The azuki bean in Korea consists of seven domestic varieties which have been developed and registered for the public during last 25 years. Here, we present a simple but reliable method to screen and identify Korean azuki bean varieties. A method based on simple sequence repeat (SSR) markers is widely used for prominent gene identification and variety discrimination. In molecular biology, real-time polymerase chain reaction (PCR) is a laboratory technique based on the polymerase chain reaction that is used to amplify and simultaneously quantify a targeted DNA molecule. It enables easy detection of a specific sequence in a DNA sample without performing electrophoresis and further processes. For separation of seven Korean azuki bean varieties, 110 unique azuki bean SSR markers from an (AG)n-enriched library were selected, synthesized and used for polymerase chain reaction (PCR). Data were taken through acrylamide gel electrophoresis and automated multi-capillary electrophoresis system for selection of specific markers and then changed into proper formats for data mining analysis. Ten primer pairs that showed high polymorphism were chosen for the indepth study. These ten primers were re-amplified with real-time PCR and checked the cycle threshold (Ct) and temperature (Tm) for comparison of amplification sequence in seven varieties. Consequently, a total of 20 alleles and 6 SSR primers were detected from the standard PCR amplification. Within these 6 primers, 7 alleles of 3 SSR primers were isolated for variety identification. From real-time PCR results, 3 SSR primers were selected as efficient markers for discrimination of seven Korean azuki bean varieties. The approach described here could be applied in monitoring our varieties and can be adapted in the azuki bean breeding program.
-
한상익,Deuk-Yong Suh,하태정,이명희,Woo-Duck Seo,오성환,장기창,Je-Bong Lee,박금룡,강항원 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4
The azuki bean in Korea consists of seven domestic varieties which have been developed and registered for the public during last 25 years. Here, we present a simple but reliable method to screen and identify Korean azuki bean varieties. A method based on simple sequence repeat (SSR) markers is widely used for prominent gene identification and variety discrimination. In molecular biology, real-time polymerase chain reaction (PCR) is a laboratory technique based on the polymerase chain reaction that is used to amplify and simultaneously quantify a targeted DNA molecule. It enables easy detection of a specific sequence in a DNA sample without performing electrophoresis and further processes. For separation of seven Korean azuki bean varieties, 110 unique azuki bean SSR markers from an (AG)n-enriched library were selected, synthesized and used for polymerase chain reaction (PCR). Data were taken through acrylamide gel electrophoresis and automated multi-capillary electrophoresis system for selection of specific markers and then changed into proper formats for data mining analysis. Ten primer pairs that showed high polymorphism were chosen for the indepth study. These ten primers were re-amplified with real-time PCR and checked the cycle threshold (Ct) and temperature (Tm) for comparison of amplification sequence in seven varieties. Consequently, a total of 20 alleles and 6 SSR primers were detected from the standard PCR amplification. Within these 6 primers, 7 alleles of 3 SSR primers were isolated for variety identification. From real-time PCR results, 3 SSR primers were selected as efficient markers for discrimination of seven Korean azuki bean varieties. The approach described here could be applied in monitoring our varieties and can be adapted in the azuki bean breeding program. The azuki bean in Korea consists of seven domestic varieties which have been developed and registered for the public during last 25 years. Here, we present a simple but reliable method to screen and identify Korean azuki bean varieties. A method based on simple sequence repeat (SSR) markers is widely used for prominent gene identification and variety discrimination. In molecular biology, real-time polymerase chain reaction (PCR) is a laboratory technique based on the polymerase chain reaction that is used to amplify and simultaneously quantify a targeted DNA molecule. It enables easy detection of a specific sequence in a DNA sample without performing electrophoresis and further processes. For separation of seven Korean azuki bean varieties, 110 unique azuki bean SSR markers from an (AG)n-enriched library were selected, synthesized and used for polymerase chain reaction (PCR). Data were taken through acrylamide gel electrophoresis and automated multi-capillary electrophoresis system for selection of specific markers and then changed into proper formats for data mining analysis. Ten primer pairs that showed high polymorphism were chosen for the indepth study. These ten primers were re-amplified with real-time PCR and checked the cycle threshold (Ct) and temperature (Tm) for comparison of amplification sequence in seven varieties. Consequently, a total of 20 alleles and 6 SSR primers were detected from the standard PCR amplification. Within these 6 primers, 7 alleles of 3 SSR primers were isolated for variety identification. From real-time PCR results, 3 SSR primers were selected as efficient markers for discrimination of seven Korean azuki bean varieties. The approach described here could be applied in monitoring our varieties and can be adapted in the azuki bean breeding program.
-
Yong-Wan Kim,Min-Je Suh,Jin-Sik Bae,Su Mi Bae,Joo Hee Yoon,Soo Young Hur,Jae Hoon Kim,Duck Young Ro,Joon Mo Lee,Sung Eun Namkoong,Chong Kook Kim,Woong Shick Ahn 대한암학회 2003 Cancer Research and Treatment Vol.35 No.4
Purpose: This study utilized both mRNA differentialdisplay and the Gene Ontology (GO) analysis to characterizethe multiple interactions of a number of geneswith gene expression profiles involved in the HPV-16-induced cervical carcinogenesis.Materials and Methods: mRNA differential displays,with HPV-16 positive cervical cancer cell line (SiHa), andnormal human keratinocyte cell line (HaCaT) as a control,were used. Each human gene has several biologicalfunctions in the Gene Ontology; therefore, several functionsof each gene were chosen to establish a powerfulcervical carcinogenesis pathway. The specific functionsassigned to these genes were then correlated with thegene expression patterns.Results: The results showed that 157 genes were upordown-regulated at least 2-fold and organized intoreciprocally dependent sub-function sets, depending ontheir cervical cancer pathway, suggesting the potentiallysignificant genes of unknown function affected by theHPV-16-derived pathway. The GO analysis suggested thatthe cervical cancer cells underwent repression of thecancer-specific cell adhesive properties. Also, genesbelonging to DNA metabolism, such as DNA repair andreplication, were strongly down-regulated, whereas significantincreases were shown in the protein degradationand synthesis.Conclusion: The GO analysis can overcome the complexityof the gene expression profile of the HPV-16-associated pathway, identify several cancer-specific cellularprocesses and genes of unknown function. It couldalso become a major competing platform for the genomewidecharacterization of carcinogenesis. (Cancer Res Treat. 2003;35:304-313)
ScienceON
스콜라연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
