초음파 유도하 맘모톰 생검을 이용한 병변의 완전제거 : 6개월 추적 초음파 검사를 통해 알아본 유용성

김혜성,한부경,최연현,김지영,고영혜,양정현,남석진,김정한 대한초음파의학회 2004 ULTRASONOGRAPHY Vol.23 No.1

Use of Dexibuprofen in pregnancy and the fetal and neonatal outcomes

( Hye Yeon Boo ),( Hyun Mi Lee ),( Yeon Kyung Cho ),( Jung Yeol Han ),( June Seek Choi ) 대한산부인과학회 2021 대한산부인과학회 학술대회 Vol.107 No.-

Cell-free DNA screening in twin pregnancies

Hye Yeon Boo,You Jung Han 대한산부인과학회 2024 Obstetrics & Gynecology Science Vol.67 No.2

Cell-free DNA (cfDNA) screening for fetal aneuploidies is clinically available and exhibits better performance than conventional serum screening tests. However, data on the clinical performance of cfDNA screening in twin pregnancies are limited. In this review, we summarized the clinical performance and evaluated the feasibility of cfDNA screening in twin pregnancies based on recent studies and recommendations. The performance of cfDNA screening for trisomy 21 in twin pregnancies is similar to that in singleton pregnancies. Specifically, cfDNA screening has a higher detection rate and lower false-positive rate compared with conventional serum screening. Consequently, recent international guidelines from several academic communities have recommended that cfDNA screening for aneuploidy in twin pregnancies could be considered. Moreover, twin pregnancies can present with specific conditions, such as different zygosities and vanishing twins; therefore, individualized counseling and management are required. Further clinical studies with more twin pregnancies are required for a more accurate analysis. Cell-free DNA (cfDNA) screening for fetal aneuploidies is clinically available and exhibits better performance than conventional serum screening tests. However, data on the clinical performance of cfDNA screening in twin pregnancies are limited. In this review, we summarized the clinical performance and evaluated the feasibility of cfDNA screening in twin pregnancies based on recent studies and recommendations. The performance of cfDNA screening for trisomy 21 in twin pregnancies is similar to that in singleton pregnancies. Specifically, cfDNA screening has a higher detection rate and lower false-positive rate compared with conventional serum screening. Consequently, recent international guidelines from several academic communities have recommended that cfDNA screening for aneuploidy in twin pregnancies could be considered. Moreover, twin pregnancies can present with specific conditions, such as different zygosities and vanishing twins; therefore, individualized counseling and management are required. Further clinical studies with more twin pregnancies are required for a more accurate analysis.

Endoplasmic Reticulum Stress and Apoptosis of Somatic Cell Nuclear Transfer Porcine Embryos

Hye-Bin Park,Hwa-Yeon Lee,Seunghyung Lee,Choon-Keun Park,Boo-Keun Yang,Hee-Tae Cheong 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10

This study was conducted to investigate the endoplasmic reticulum (ER) stress and subsequent apoptosis of porcine somatic cell nuclear transfer (SCNT) embryos induced during SCNT procedures, which compared with IVF embryos. Porcine SCNT embryos were made by micromanipulation and electrofusion/activation. Control porcine embryos were prepared by in vitro fertilization (IVF). Porcine SCNT and IVF embryos were sampled at 3 h-, and 20 h-post SCNT or IVF, and at the blastocyst stage for mRNA extraction. The x-box binding protein 1 (Xbp1) mRNA, the key transcription factor in the ER stress condition was confirmed by RT-PCR analysis. The expressions of ER stress-associated genes, the C/EBP homologous protein (CHOP), binding protein (BiP), activating transcription factor 4 (ATF4), and glucose-regulated protein 94 (GRP94) were confirmed by RT-qPCR. Apoptotic gene expression was analyzed by RT-PCR. Prior to SCNT, the splicing of Xbp1 mRNA and increased expressions of ER stress-associated genes were confirmed in the somatic cells treated with tunicamycin (TM), an ER stress inducer. Expression of spliced Xbp1 (Xbp1s) mRNA in the SCNT embryos did not differ with that of IVF embryos, regardless of the embryonic stage. Expression of ER stress-associated genes in SCNT embryos was, however, significantly increased in the all embryonic stages compared to IVF embryos (p<0.05). Apoptotic gene expression was slightly high in the SCNT embryos, but not different from that of IVF embryos in each group. The result of this study indicates that excessive ER stress can be induced by SCNT procedures. Although, ER stress-derived apoptosis was not identified in this study, more research studies are needed on the relationship between SCNT and ER stress-derived apoptosis.

Phase changeable silver selenide thin films fabricated by pulse electrodeposition

Boo Hyun An,Hye Min Ji,Jun-Hua Wu,Moon Kyu Cho,Ki-Yeon Yang,이헌,김영근 한국물리학회 2009 Current Applied Physics Vol.9 No.6

We have used a simple and direct method to successfully prepare Ag2Se thin films by pulse electrodeposition from a single aqueous solution of Ag and Se ions. The composition analysis confirms the stoichiometry and the morphological characterization shows that the thin films are dense and smooth. The films have the orthorhombic structure of regular Ag2Se materials at room temperature, while the electrical measurements reveal an interesting electrical behavior, similar to that of the Ag2Se nanowire arrays. The Ag2Se thin films were successfully deposited into polymer templates to form well-defined Ag2Se nanostructure, which provides a possibility to apply the method to high density, non-volatile memories based on phase change materials.

Endoplasmic Reticulum (ER) Stress and Apoptosis in Parthenogenetic Porcine Embryos following Different Combination of Activation Methods

Hye-Bin Park,Yeo-Reum Park,Hwa-Yeon Lee,Hyo-Kyung Bae,Seunghyung Lee,Choon-Keun Park,Boo-Keun Yang,Hee-Tae Cheong 한국수정란이식학회 2017 한국동물생명공학회지 Vol.32 No.1

This study was conducted to investigate the effect of activation method on the endoplasmic reticulum (ER) stress induction, apoptosis and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by four activation methods; 1) electric stimulus (ES) (E), 2) ES+10 μM Ca-ionophore (A23187) treatment (EC), 3) ES+2 mM 6-dimethylaminopurine (6-DMAP) treatment (ED), or 4) ES+A23187 and 6-DMAP treatments (ECD). Parthenogenetic embryos were sampled to analyze x-box binding protein 1 (Xbp1) mRNA, ER stress-associated genes and apoptosis genes at 3 h after ES and the 1-cell and blastocyst stages. In the EC group, the band intensity of spliced Xbp1 (Xbp1s) mRNA was higher than those of the other groups at the 3 h and 1-cell stage, and higher than that of the E group at the blastocyst stage. Four ER stress-associated genes were expressed at the highest level in the EC group and weakly expressed in the ED group at 3 h after activation. However, most of the genes were highly expressed at the 1-cell and blastocyst stages with some variation in the EC and ECD groups. Expression of Bcl-2-associated X protein (Bax) and caspase-3 mRNA was significantly higher in the EC group than in the other groups at all development stages. The developmental rates to the blastocyst stage were higher in the ED and ECD groups than in the E and EC groups. These results suggest that the intracellular ER stress of parthenogenetic porcine embryos is affected by the activation method and subsequently lead to the apoptosis of embryos.

Proteome Profiling Unfurl Differential Expressed Proteins from Various Explants in Platycodon Grandiflorum

Hye-Rim Kim,Soo-Jeong Kwon,Swapan Kumar Roy,Seong-Woo Cho,Hag-Hyun Kim,Kab-Yeon Cho,Hee-Ock Boo,Sun-Hee Woo 한국작물학회 2015 Korean journal of crop science Vol.60 No.1

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (≥ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose- 1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

가정용 IoT 네트워크에서의 이상 징후 탐지 솔루션 제안

박연진 ( Yeon-jin Park ),오주혜 ( Ju-hye Oh ),이근호 ( Keun-ho Lee ),전유부 ( You-boo Jeon ) 한국정보처리학회 2016 한국정보처리학회 학술대회논문집 Vol.23 No.2

인터넷의 발달이 증대함에 따라 컴퓨터를 전문적으로 사용하지 않더라도 가정에서 NAS등의 서버모델을 사용하는 경우가 많아졌다. 한번 구매하면 저전력으로 손쉽게 사용할 수 있는 대용량 서버모델의 사용자 수가 점차적으로 증가하고 있다. 이와 동시에 간단한 검색만으로 구할 수 있는 웹과 네트워크에 큰 악영향을 미치는 악성도구들도 인터넷상에 퍼지고 있다. 쉽게 얻은 해킹 도구로 간소하게 설치된 가정용 서버 등을 공격하는 빈도수가 점점 늘어나고 있는 추세이다. 본 연구는 가정용 IoT 서버 및 네트워크에서 이상 징후를 탐지하는 솔루션 모델의 구축을 제안하고자 한다.

Massive MIMO 시스템에서 딥 러닝을 이용한 모델 기반 신호 검출 기법 연구

장준용(Jun-Yong Jang),신범식(Beom-Sik Shin),오지혜(Ji-Hye Oh),임승우(Seung-Woo Im),송형규(Hyoung-Kyu Song) 대한전자공학회 2021 대한전자공학회 학술대회 Vol.2021 No.6

In this paper, an additional study about the exiting MMNet for data detection using Deep Learning (DL) is provided. Existing detection methods are unable to apply massive Multiple Input Multiple Output (MIMO) systems due to high complexity. Therefore, new detection methods with moderate complexity and high error rate performance have been studied until a recently date. In order to solve above problem, DL based detection method has been studied and MMNet is successful model-driven detection method with high adaptive capability. This paper presents a new approach to the denoiser of the nonlinear process for MMNet.

Endoplasmic Reticulum (ER) Stress and Apoptosis in Parthenogenetic Porcine Embryos following Different Combination of Activation Methods

Park, Hye-Bin,Park, Yeo-Reum,Lee, Hwa-Yeon,Bae, Hyo-Kyung,Lee, Seunghyung,Park, Choon-Keun,Yang, Boo-Keun,Cheong, Hee-Tae The Korean Society of Embryo Transfer 2017 한국동물생명공학회지 Vol.32 No.1

This study was conducted to investigate the effect of activation method on the endoplasmic reticulum (ER) stress induction, apoptosis and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by four activation methods; 1) electric stimulus (ES) (E), 2) $ES+10{\mu}M$ Ca-ionophore (A23187) treatment (EC), 3) ES+2 mM 6-dimethylaminopurine (6-DMAP) treatment (ED), or 4) ES+A23187 and 6-DMAP treatments (ECD). Parthenogenetic embryos were sampled to analyze x-box binding protein 1 (Xbp1) mRNA, ER stress-associated genes and apoptosis genes at 3 h after ES and the 1-cell and blastocyst stages. In the EC group, the band intensity of spliced Xbp1 (Xbp1s) mRNA was higher than those of the other groups at the 3 h and 1-cell stage, and higher than that of the E group at the blastocyst stage. Four ER stress-associated genes were expressed at the highest level in the EC group and weakly expressed in the ED group at 3 h after activation. However, most of the genes were highly expressed at the 1-cell and blastocyst stages with some variation in the EC and ECD groups. Expression of Bcl-2-associated X protein (Bax) and caspase-3 mRNA was significantly higher in the EC group than in the other groups at all development stages. The developmental rates to the blastocyst stage were higher in the ED and ECD groups than in the E and EC groups. These results suggest that the intracellular ER stress of parthenogenetic porcine embryos is affected by the activation method and subsequently lead to the apoptosis of embryos.