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      • 황 첨착 활성탄에 의한 구리제거

        김영환,박정훈,김정섭,유현철,이봉섭,곽명화,우성훈,박승조 東亞大學校 環境問題硏究所 2005 硏究報告 Vol.27 No.1

        This study was carried out to remove copper ion in case of activated carbon impregnated sulphur(ACS) dose and different pH using activated carbon impregnated sulphur that prepared from activated carbon at sulphur dioxide atmosphere. Removal copper ion concentration was increased as increasing the ACS dose when copper ion concentration was 10 mg/L. Optimum dose of ACS 13.3 g ACS/L and maximum removal efficiency was 93%. The results obtained from adsorption experiment in the condition of ACS optimum dose when the pH was 3, 4, 5 respectively was pH 5 was excellent.

      • KCI등재후보

        GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

        김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

        Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

      • KCI등재후보

        GC/NPD를 이용한 다성분 잔류농약의 동시분석

        김우성,이선화,김상엽,정동윤,김재이,이영자,이홍재,정성욱,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

        Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with fiorisil, and finally analyzed simultaneously the organophosphorus pesticides using GC/NPD, Ultra-2 and Ultra-1 fused silica capillary columns were used to separate and identify the products. Recovery of most analytes from soybean sample, taken from pesticide residues well, was greater than(80%) for all except(6) analytes. This method can simultaneously determine multiple pesticides with a high degree of accuracy and precision.

      • SCOPUSKCI등재

        대장암 주변에서 관찰되는 백색반점의 병리조직학적 특성과 임상적 의의

        김효종,조진만,장린,동석호,김윤화,장영운,박일랑,김병호,이정일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.4

        Background/Aims: To investigate the difference between colorectal adenocarcinomas with white spots (foamy cells) and those without white spots, clinically and histopathologically, were examined 37 cases of colorectal adenocarcinomas were classified in this study. Methods: Two groups: those with white spots and those without. In each case, evidence of lymph node and liver metastasis was sought. Immunoreactive staining for macrophage was performed. Results: Increased incidence of exophytic tumors was found (73%, p=0.028) in the group with white spots. The incidences of lymph node metastasis were 33% vs. 45% between the group with white spots and the group of without, but the difference was not statistically significant. Conclusions: These results suggested that foamy cells might have a beneficial role in colorectal cancer, although they were not statistically significant. Therefore, further prospective study be warranted.

      • 방사선 센서의 동작특성

        임영화,한상효,김도성,강희동 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

        A Geiger-M□ller(GM) radiation sensor is fabricated by using the vacuum system and gas feeding system. The base pressure of the vacuum system is 1X10^(-7)torr, and two MFCs(0~10sccm, ±1% accuracy of the full scale) are used to control the composition of filling gas exactly. The fabricated GM radiation sensor is simultaneously filled with the mixture of neon and clorine. The mica window(7.43mg/cm^(2)) is attached to the GM radiation sensor. The optimized GM radiation sensor has about 660V starting voltage, 125V plateua length, and 10%/100V plateau slope.

      • KCI등재
      • 장구균의 동정에 대한 PCR 방법의 유용성

        최연화,이영선,김홍빈,김치경,김봉수 대한감염학회 2001 감염 Vol.33 No.2

        Background : Enterococci are important cause of nosocomial infections. Recently, vancomycin-resistant enterococci (VRE) has been increasingly reported as significant nosocomial pathogens. Therefore, accurate identification of enterococcal species is a prerequisite step for the appropriate antibiotic treatment and epidemiologic surveillance. We wanted to know the usefulness of PCR method compared with Vitek automatic identification system. Methods : Totally 105 isolates were identified on the species level by Vitek (GPI card and software version R06.1), methyl-α -D-glucopyranoside test, and PCR methods. Results : Among 105 enterococcal isolates, 59 were identified as E. faecium, 11 E. faecalis, 6 E. gallinarum by Vitek. But 29 isolates (28%) were unidentified. Subsequently all of these isolates were analyzed by PCR, the results of which were as follows : 17 E. faecium, 5 E. casseliflavus, 7 E. gallinarum. Two isolates identified as E. gallinarum by Vitek were reidentified as E. casseliflavus by PCR and other methods far phenotypic characterization. Conclusoin : PCR method was more accurate and sensitive than Vitek for the identification of enterococci species. (Korean J Infect Dis 33:123∼127, 2001)

      • 재이식 가능한 32비트 테스크 코어 설계

        윤영상,최종화,김용대,한선경,유영갑 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.1

        본 논문에서 제시한 32비트 테스트 코어는 기존의 ARM과 같은 32비트 RISC 코어를 대신하여 JTAG 및 코어브레이커의 회로 테스트를 위하여 고안되었다. 테스트 코어는 디버깅유닛을 바꾸어가며 재이식(retargetable) 가능하게 사용될 수 있다는 장점을 갖는다. 회로 합성 결과 하드웨어는 ARM과 비교하여 33% 정도의 게이트를 가진다. 테스트 코어는 보다 높은 디버깅 성능을 위해 다중로드(multiple load; LDMA)와 다중저장(multiple store; STMA)명령을 추가하였으며 단일로드(single load; LDR) 또는 단일저장(single store; STR)명령들에 비해 64% 정도의 처리시간 단축을 나타내었다. A 32bit RISC type core is designed to replace full processors for testing JTAG and core breaker modules. It is retargetable and can be utilized to test a debugging unit. The design uses about 33% of gates with respect to a fully designed ARM7. It has instructions, multiple load or store(LDMA, STMA), for debugging purposes. Simulation result shows that they reduce the execution time about 64% with respect to processors having a single load or store(LDR, STR) instruction.

      • KCI등재

        Failure Cost와 Life Cycle Cost를 고려한 연속시스템에 대한 최적 장치 선택에 관한 연구

        진상화,김용하,송희열,여영구,김인원 한국안전학회 2004 한국안전학회지 Vol.19 No.4

        In this study, the required life cycle cost is evaluated in consideration of the equipment's availability during its life cycle. In order to meet the maximum availability required by the process, the failure cost and life cycle cost is assessed, The optimal equipment selection method is presented according to the analysis of the failure cost and life cycle cost. For the systems in which equipments are connected serially, the optimal equipments are selected by minimizing the life cycle cost and satisfying the required system availability goal. In addition, the selection methods and life cycle cost are analyzed according to the cost variation of the equipment. By using the life cycle evaluation procedure, the failure cost and maintenance cost needed during the life cycle of the equipment can be presented.

      • 인삼 Peroxidase(PgPrx3) 유전자의 분리 및 연초의 형질전환

        손화,심주선,김세영,노영덕,김무성,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

        Peroxidase는 항산화에 관련된 효소이며 식물의 환경스트레스와 성장에 관련되는 중요한 유전자이다. 본 실험에서 사용된 Peroxidase Ⅲ의 cDNA는 Panax ginseng C.A. Meyer 잎에서 추출하였으며 PgPrx3라 명명하였다. 이 gene의 ORF영역은 1,065 bP이고 355개의 아미노산을 가지고 있다. BioEdit 프로그램을 이용하여 PgPrx3와 다른 식물의 peroxidase gene과 비교한 결과 상동성을 가지고 있었으며, Spinacia oleracea(70%), Vigna angulais(71%), Nicotiana tabacum(69%) and Linum usitatissimum(65%). 그중 Vigna angularis의 상동성이 가장 높았다. 담배에 본 유전자를 형질전환시켜 유전자의 특성을 검정하기 위하여 35S/35S/AMV/peroxidas/Tnos를 벡터 pRD400에 재조합 후 아그로박테리아를 이용하여 담배에 도입시켰다. 그리고 PCR를 이용하여 유전자 PgPrx3가 담배에 도입된 것을 확인하였으며, RT-PCR로 정상적으로 PgPrx3 유전자가 전사되어 RNA를 생성하고 있음을 확인하였다. A peroxidase (E.C. 1.11.1.7) is very important enzyme as antioxidants. The function of this gene is connected with growth and environmental stress of plant. A class Ⅲ peroxidase cDNA was isolated from the leaf of Panax ginseng C.A. Meyer and named as PgPrx3 which is consisted of an ORF (open reading frame) of 1,065 bp and an amino acid of 355 residues. BioEdit software was used to compare the PgPrx3 amino acid sequence with other plants that showed homologies with Spinacia oleracea (70%), Vigna angularis (71%), Nicotiana tabacum (69%) and Linum usitatissimum (65%). The peroxidase of Vigna angulatis was the most homologous with ginseng. The chimeric PgPrx3 gene, 35S/35S/AMV/peroxidas/ Tnos, was constructed in the binary vector pRD400. Agrobacterium tumefaciens strain LBA4404 harboring disarmed Ti-plasmid was used to transform Nicotiana tabacum L. using the leaf disc. Incorporation of the chimeric gene PgPrx3 into plants were confirmed by PCR analysis of genomic DNA and RT-PCR analysis of mRNA from transgenic tobacco. The PgPrx3 gene from ginseng was stably expressed in transgenic tobacco plants.

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