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Jeong, Haeng-Soon,Jeong, In-Chel,Kim, Andre,Kang, Shin-Won,Kang, Ho Sung,Kim, Yung-Jin,Lee, Suk-Hee,Park, Jang-Su 부산대학교 유전공학연구소 2002 분자생물학 연구보 Vol.18 No.-
Eukaryotic replication protein A (RPA) is a single-stranded(ss) DNA binding protein with multiple functions in DNA replication, repair, and genetic recombination. The 70-kDa subunit of eukaryotic RPA contains a conserved four cysteine-type zinc-finger motif that has been implicated in the regulation of DNA replication and repair. Recently, we described a novel function for the zine-finger motif in the regulation of human RPA's ssDNA binding activity through reduction-oxidation (redox). Here, we show that yeast RPA's ssDNA binding activity is regulated by redox potential through its RPA32 and/or RPA14 subunits. Yeast RPA requires a reducing agent, such as dithiothreitol (DTT), for its ssDNA binding activity. Also, under non-reducing conditions, its DNA binding activity decreases 20 fold. In contrast, the RPA70 subunit does not require DTT for its DNA binding activity and is not affected by the redox condition. These results suggest that all three subunits are required for the regulation of RPA's DNA binding activity through redox potential.
Jeong, Haeng-Soon,Jeong, In-Chel,Kim, Andre,Kang, Shin-Won,Kang, Ho-Sung,Kim, Yung-Jin,Lee, Suk-Hee,Park, Jang-Su Korean Society for Biochemistry and Molecular Biol 2002 Journal of biochemistry and molecular biology Vol.35 No.2
Eukaryotic replication protein A (RPA) is a single-stranded(ss) DNA binding protein with multiple functions in DNA replication, repair, and genetic recombination. The 70-kDa subunit of eukaryotic RPA contains a conserved four cysteine-type zinc-finger motif that has been implicated in the regulation of DNA replication and repair. Recently, we described a novel function for the zinc-finger motif in the regulation of human RPA's ssDNA binding activity through reduction-oxidation (redox). Here, we show that yeast RPA's ssDNA binding activity is regulated by redox potential through its RPA32 and/or RPA14 subunits. Yeast RPA requires a reducing agent, such as dithiothreitol (DTT), for its ssDNA binding activity. Also, under non-reducing conditions, its DNA binding activity decreases 20 fold. In contrast, the RPA 70 subunit does not require DTT for its DNA binding activity and is not affected by the redox condition. These results suggest that all three subunits are required for the regulation of RPA's DNA binding activity through redox potential.
고구마 괴근과 잎자루 식이섬유의 항산화 효과 및 인간 대장암세포에 대한 항암작용
정행순 ( Haeng Soon Jeong ),주현 ( Hyun Joo ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2013 공업화학 Vol.24 No.5
Contents of the total polyphenols and flavonoids in the dietary fiber from tubers and stalks of domestic sweet potatoes were investigated. In addition, their antioxidant activity as well as the potent anti-cancer effects through the growth inhibition in human colon cancer cells (HT-29) in vitro were tested. The total flavonoids as naringin equivalents in dietary fiber from tubers and stalks of sweet potatoes were 0.5 ± 0.001 naringin/g extract and 2.0 ± 0.008 mg naringin/g extract dry basis, respectively. The amounts of the total polyphenols as gallic acid equivalents were 2.8 ± 0.01 mg gallic acid/g dry basis and 6.3 ± 0.03 mg gallic acid/g dry basis, respectively. 1,2-Diphenyi-1-picrylhydrazyl (DPPH) radical-scavenging activity of the dietary fiber from stalks was 2.4 times higher than that of the dietary fiber from tubers. Interestingly, a strong growth inhibition on HT-29 cells was observed in both dietary fibers originated from stalks and tubers of sweet potato in a dose-dependent manner. In addition, we found that the dietary fiber from tubers and stalks of sweet potato increased the gene expression of tumor suppressor p53. The great potential value in the prevention of various diseases including cancer the potential value could be confirmed through effects of the dietary fiber from tubers and stalks of sweet potato on antioxidant activity and anticancer in human colon cancer.
추출법에 따른 큰실말(Cladosiphon novae-caledoniae kylin) 부산물식이섬유의 항산화 효과 및 HT-29 대장암 세포에 대한 항암 작용
정행순 ( Haeng Soon Jeong ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2014 공업화학 Vol.25 No.4
In this study, the dietary fiber contents of Mozuku (Cladosiphon novae-caledoniae kylin) residue and the extraction condition(HCl, H2SO4, NaOH, Na2CO3, Na2EDTA) of the dietary fiber was investigated. We examined that the contents of the totalpolyphenols and flavonoids in the dietary fiber from Mozuku residue, and the potent anti-cancer effect was also tested throughthe growth inhibition in human colon cancer cells (HT-29) in vitro. It was effective to extract soluble dietary fiber with 1.5%Na2EDTA and 0.05 N HCl in Mozuku residue. The extraction time and temperature affected the yields of soluble dietaryfiber. The contents of the total polyphenols and flavonoids in the dietary fiber from Mozuku residue were the highest in 1%NaOH extract (Total polyphenols 34.4 ± 0.055 mg gallic acid/g dry basis, total flavonoids 34.7 ± 0.023 mg naringin/g extractdry basis). In DPPH radical scavenging activity, 1% NaOH extract showed the most potent antioxidant activity. In the resultof viability in human colon cancer cells, growth inhibition was observed in D.W., 0.05 N HCl, and 0.5% Na2CO3 extractsin a dose-dependent manner. These results demonstrated that soluble dietary fiber from Mozuku residue significant antioxidantactivity and anticancer in human colon cancer.
Park, Sung‐,Chul,Kim, Yun‐,Hee,Kim, Sun Ha,Jeong, Yu Jeong,Kim, Cha Young,Lee, Joon Seol,Bae, Ji‐,Yeong,Ahn, Mi‐,Jeong,Jeong, Jae Cheol,Lee, Haeng‐,Soon,Kwak, Sang‐ Blackwell Publishing Ltd 2015 Physiologia plantarum Vol.153 No.4
<P>The R2R3-type protein IbMYB1 is a key regulator of anthocyanin biosynthesis in the storage roots of sweet potato [Ipomoea batatas (L.) Lam]. Previously, we demonstrated that IbMYB1 expression stimulated anthocyanin pigmentation in tobacco leaves and Arabidopsis. Here, we generated dual-pigmented transgenic sweet potato plants that accumulated high levels of both anthocyanins and carotenoids in a single sweet potato storage root. An orange-fleshed cultivar with high carotenoid levels was transformed with the IbMYB1 gene under the control of either the storage root-specific sporamin 1 (SPO1) promoter or the oxidative stress-inducible peroxidase anionic 2 (SWPA2) promoter. The SPO1-MYB transgenic lines exhibited higher anthocyanin levels in storage roots than empty vector control (EV) or SWPA2-MYB plants, but carotenoid content was unchanged. SWPA2-MYB transgenic lines exhibited higher levels of both anthocyanin and carotenoids than EV plants. Analysis of hydrolyzed anthocyanin extracts indicated that cyanidin and peonidin predominated in both overexpression lines. Quantitative reverse transcription-polymerase chain reaction analysis demonstrated that IbMYB1 expression in both IbMYB1 transgenic lines strongly induced the upregulation of several genes in the anthocyanin biosynthetic pathway, whereas the expression of carotenoid biosynthetic pathway genes varied between transgenic lines. Increased anthocyanin levels in transgenic plants also promoted the elevation of proanthocyanidin and total phenolic levels in fresh storage roots. Consequently, all IbMYB1 transgenic plants displayed much higher antioxidant activities than EV plants. In field cultivations, storage root yields varied between the transgenic lines. Taken together, our results indicate that overexpression of IbMYB1 is a highly promising strategy for the generation of transgenic plants with enhanced antioxidant capacity.</P>
정재심,최정화,이순행,김양수 대한기초간호자연과학회 2003 Journal of korean biological nursing science Vol.5 No.2
The effect of hand hygiene was measured by hand culture before and after hand hygiene for 86 nurses, doctors, and nurses aide/housekeepers in Surgical Intensive Care Unit. The subjects were asked to press their dominant hand in hand-shaped Mannitol salt agar immediately after patient contact and then washed their hand by preferred hand hygiene agents [soap and water, waterless alcohol gel, or 4% chlorhexidine gluconate detergent(CHG)], and cultured one hand again. Amount of isolated microorganism was calculated by counting the number of divided areas(1 x 1cm) which is culture positive in hand culture plate. The amount of microorganisms were significantly reduced from 58.1(±38.59) to 27.4(±30.4) cells after hand hygiene. The staff nurse's hand hygiene was more effective compared to medical doctors and nurses aide/housekeepers. Methicillin-resistant Staphylococcus aureus(MRSA) was isolated in 41(47.1%) subjects ; but only removed 100% in 28(32.2%) subjects. When the amount of hand microorganisms was compared by subject's preferred hand hygiene agents, it was decreased in order of 4% CHG, waterless alcohol solution, soap and water, and water. The hand hygiene practice was inadequate to reduce hand microorganisms and significantly different by occupations. Further research and development of hand hygiene improvement program which emphasize the quality of hand hygiene is recommended.