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RISS 인기검색어
- 검색키워드
- 저자 : 정행순 ( Haeng Soon Jeong ) (검색결과 4 건)
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추출법에 따른 큰실말(Cladosiphon novae-caledoniae kylin) 부산물식이섬유의 항산화 효과 및 HT-29 대장암 세포에 대한 항암 작용
정행순 ( Haeng Soon Jeong ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2014 공업화학 Vol.25 No.4
In this study, the dietary fiber contents of Mozuku (Cladosiphon novae-caledoniae kylin) residue and the extraction condition(HCl, H2SO4, NaOH, Na2CO3, Na2EDTA) of the dietary fiber was investigated. We examined that the contents of the totalpolyphenols and flavonoids in the dietary fiber from Mozuku residue, and the potent anti-cancer effect was also tested throughthe growth inhibition in human colon cancer cells (HT-29) in vitro. It was effective to extract soluble dietary fiber with 1.5%Na2EDTA and 0.05 N HCl in Mozuku residue. The extraction time and temperature affected the yields of soluble dietaryfiber. The contents of the total polyphenols and flavonoids in the dietary fiber from Mozuku residue were the highest in 1%NaOH extract (Total polyphenols 34.4 ± 0.055 mg gallic acid/g dry basis, total flavonoids 34.7 ± 0.023 mg naringin/g extractdry basis). In DPPH radical scavenging activity, 1% NaOH extract showed the most potent antioxidant activity. In the resultof viability in human colon cancer cells, growth inhibition was observed in D.W., 0.05 N HCl, and 0.5% Na2CO3 extractsin a dose-dependent manner. These results demonstrated that soluble dietary fiber from Mozuku residue significant antioxidantactivity and anticancer in human colon cancer.
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고구마 괴근과 잎자루 식이섬유의 항산화 효과 및 인간 대장암세포에 대한 항암작용
정행순 ( Haeng Soon Jeong ),주현 ( Hyun Joo ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2013 공업화학 Vol.24 No.5
Contents of the total polyphenols and flavonoids in the dietary fiber from tubers and stalks of domestic sweet potatoes were investigated. In addition, their antioxidant activity as well as the potent anti-cancer effects through the growth inhibition in human colon cancer cells (HT-29) in vitro were tested. The total flavonoids as naringin equivalents in dietary fiber from tubers and stalks of sweet potatoes were 0.5 ± 0.001 naringin/g extract and 2.0 ± 0.008 mg naringin/g extract dry basis, respectively. The amounts of the total polyphenols as gallic acid equivalents were 2.8 ± 0.01 mg gallic acid/g dry basis and 6.3 ± 0.03 mg gallic acid/g dry basis, respectively. 1,2-Diphenyi-1-picrylhydrazyl (DPPH) radical-scavenging activity of the dietary fiber from stalks was 2.4 times higher than that of the dietary fiber from tubers. Interestingly, a strong growth inhibition on HT-29 cells was observed in both dietary fibers originated from stalks and tubers of sweet potato in a dose-dependent manner. In addition, we found that the dietary fiber from tubers and stalks of sweet potato increased the gene expression of tumor suppressor p53. The great potential value in the prevention of various diseases including cancer the potential value could be confirmed through effects of the dietary fiber from tubers and stalks of sweet potato on antioxidant activity and anticancer in human colon cancer.
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자외선 조사에 의한 지질 고생산성 Chlorella vulgaris 변이주 분리
정행순,최민경,최태오,이재화,Jeong, Haeng Soon,Choi, Min Kyung,Choi, Tae-O,Lee, Jae-Hwa 한국해양바이오학회 2014 한국해양바이오학회지 Vol.6 No.1
Chlorella vulgaris, a genus of single-cell green algae, is considered to be a very essential resource for the higher value-added business including functional food and biodiesel, due to its high contents of protein, carbohydrate and lipid. In this study, ultraviolet rays were irradiated in order to induce the mutation of C. vulgaris. After inducing the mutation, UV1-20 mutant, high in lipid was selected and its cell growth rate, dry weight, pigment content and lipid content were measured. The growth rate of the UV1-20 mutant was increased almost 1.5 times than the wild type, but pigment contents of chlorophyll and carotinoid were decreased. In addition, the lipid content of UV1-20 was increased 1.8 times than the wild type. Therefore, C. vulgaris mutant, isolated in this study, is considered to have sufficient potential to be used as a material for the higher value-added business.
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연구 논문 : 슬러지 냉동처리에 의한 갈조로부터 바이오가스 생산
김지윤 ( Ji Youn Kim ),정행순 ( Haeng Soon Jeong ),우대식 ( Dae Sik Woo ),김상민 ( Sang Min Kim ),김인수 ( In Soo Kim ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2012 공업화학 Vol.23 No.6
본 연구에서는 해조류 다시마를 바이오매스로 이용하는 혐기성 소화로부터 바이오가스를 생산하였다. 최적의 혐기성 배양조건을 확립하기 위해 슬러지는 -70 ℃에서 20 min 동안 냉동 전처리를 하였다. 대조군과 비교했을 때 수소와 메탄가스는 각각 2.7배와 3.4배 증가한 667.28 mL/L와 3420.24 mL/L를 생산하였다. 냉동 전처리한 슬러지의 초기 pH 최적 조건은 7.0이었고, 다시마 바이오매스의 초기 pH 최적 조건은 8.0이었으며 알칼리 조건에서 보다 산성 조건에서 바이오가스 생산량이 크게 감소하는 것을 확인할 수 있었다. 슬러지의 냉동 전처리와 바이오매스 및 슬러지의 최적 pH 조건에서 최대 643.73 mL/L의 수소와 4291.6 mL/L의 메탄가스를 생산하였다. 이는 대조군과 비교했을 때 각각 2.6배와 4.3배 증가한 생산량이었다. 또한 최적 조건의 5-L 회분식 혐기성 배양에서 바이오가스의 생산량을 측정한 결과 기질에 포함된 다시마에 의해 생산될 수 있는 최대 생산량은 수소 1605.03 mL/L와 메탄가스 4593.71 mL/L로 확인되었다. In the present study, biogas was produced from the anaerobic digestion of marine macroalgae (Laminaria japonica) biomass. The optimal anaerobic condition for producing the sludge was the freeze treatment at -70 ℃ for 20 min. Total amounts of hydrogen and methane gas produced were 667.28 mL/L and 3420.24 mL/L, respectively, which were 2.7 and 3.4 times greater than that in the control group. Freeze treatment of sludge produced the maximum biogas under an initial optimum pH of 7.0 and the maximum biomass at an initial optimum pH of 8.0. We confirmed that biogas production was greatly reduced under acidic conditions compared to that under alkaline conditions. Sludge was freeze treated, and the biomass and sludge production was optimal the total amounts of hydrogen and methane gas produced were 643.73 mL/L and 4291.6 mL/L, respectively, which were 2.6 and 4.3 times greater than in the control group. Also the results showed that under optimal conditions in a 5-L bioreactor, a maximum of 1605.03 mL/L of hydrogen and 4593.71 mL/L of methane gas could be produced by the substrate contained in the marine macroalgae biomass.
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