부산·울산·경남지역 직업병 감시체계

김정일,김병권,김정원,채창호,이철호,강동묵,김지홍,김진하,김영욱,이영하,이지호,김정호,윤형렬,유철인,정백근,장태원,김운규,윤동영,강진욱,김종은,안진홍,이동준,장준호,이광영,송혜란,최영희,이용환,조병만,최홍렬,고상백,김은아,이유진,홍영습,정갑열,김정만,김준연 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.1

Objective: Occupational medicine specialists in the Busan, Ulsan and Kyung-Nam areas established an area-based occupational disease surveillance system and used this system to collected case information for the purpose of preventing occupational diseases Methods: l l hospital participated in this system. The authors selected five main diseases, which were hand-arm vibration syndrome (HAVS), work-related musculoskeletal disorder (WRMSD), occupational asthma, occupational skin disease and occupational and occupational lung cancer and established their case definitions. All cases were reported on the web, and real time analysis was conducted. Results: Between April 2001 and April 2003, 192 cases of HAVS, 118 cases of WRMSD, 33 cases of occupational asthma, 17 cases of occupational skin disease and 17 cases of occupational lung cancer (for a total of 377 cases of these five main diseases) were reported. most of the HAVS cases came from shipbuilding, and 172 of them (92.7%) were associated with grinding. Of the four main types of WRMSD, carpal tunnel syndrome (CTS) was the most prevalent with 46 cases and shipbuilding was also the main industry involved (83.9%). The ergonomic risk factors involved mainly associated with the hand. In 19 (57.6%) and 4 (12.1%) cases of occupational asthma, the agents involved. The causative agents of occupational lung cancer included Cr, welding fume, PAH, Ni, etc. 10(58.8%) of the cases were diagnosed as squamous cell carcinoma and 5(29.4%) as adenoma. Conclusion: This result showed that an area-based occupational disease surveillance system might provide an effective method of evaluating the prevalence of such diseases, however the Busan, Ulsan and Kyung-Nam provinces are too large to be treated as individual areas. Therefore, the authors suggest that each province should establish its own surveillance system.

Loss-of-function screens of druggable targetome against cancer stem–like cells

Song, Mee,Lee, Hani,Nam, Myung-Hee,Jeong, Euna,Kim, Somin,Hong, Yourae,Kim, Nayoung,Yim, Hwa Young,Yoo, Young-Ji,Kim, Jung Seok,Kim, Jin-Seok,Cho, Yong-Yeon,Mills, Gordon B.,Kim, Woo-Young,Yoon, Sukjo Federation of American Societies for Experimental 2017 The FASEB Journal Vol.31 No.2

<P>Cancer stem–like cells (CSLCs) contribute to the initiation and recurrence of tumors and to their resistance to conventional therapies. In this study, small interfering RNA (siRNA)-based screening of ∼4800 druggable genes in 3-dimensional CSLC cultures in comparison to 2-dimensional bulk cultures of U87 glioma cells revealed 3 groups of genes essential for the following: survival of the CSLC population only, bulk-cultured population only, or both populations. While diverse biologic processes were associated with siRNAs reducing the bulk-cultured population, CSLC-eliminating siRNAs were enriched in a few functional categories, such as lipid metabolism, protein metabolism, and gene expression. Interestingly, siRNAs that selectively reduced CSLC only were found to target genes for cholesterol and unsaturated fatty acid synthesis. The lipidomic profile of CSLCs revealed increased levels of monounsaturated lipids. Pharmacologic blockage of these target pathways reduced CSLCs, and this effect was eliminated by addition of downstream metabolite products. The present CSLC-sensitive target categories provide a useful resource that can be exploited for the selective elimination of CSLCs.—Song, M., Lee, H., Nam, M.-H., Jeong, E., Kim, S., Hong, Y., Kim, N., Yim, H. Y., Yoo, Y.-J., Kim, J. S., Kim, J.-S., Cho, Y.-Y., Mills, G. B., Kim, W.-Y., Yoon, S. Loss-of-function screens of druggable targetome against cancer stem–like cells.</P>

Improved photovoltaic performance of inverted polymer solar cells through a sol-gel processed Al-doped ZnO electron extraction layer.

Kim, Jun Young,Cho, Eunae,Kim, Jaehoon,Shin, Hyeonwoo,Roh, Jeongkyun,Thambidurai, Mariyappan,Kang, Chan-Mo,Song, Hyung-Jun,Kim, SeongMin,Kim, Hyeok,Lee, Changhee Optical Society of America 2015 Optics express Vol.23 No.19

<P>We demonstrate that nanocrystalline Al-doped zinc oxide (n-AZO) thin film used as an electron-extraction layer can significantly enhance the performance of inverted polymer solar cells based on the bulk heterojunction of poly[[9-(1-octylnonyl)-9H-carbazole-2,7-diyl]-2,5-thiophenediyl-2,1,3-benzothiadiazole-4,7-diyl-2,5-thiophenediyl] (PCDTBT) and [6,6]-phenyl C₇₁-butyric acid methyl ester (PC₇₀BM). A synergistic study with both simulation and experiment on n-AZO was carried out to offer a rational guidance for the efficiency improvement. As a result, An n-AZO film with an average grain size of 13 to 22 nm was prepared by a sol-gel spin-coating method, and a minimum resistivity of 2.1 ?? 10^-3 곽·cm was obtained for an Al-doping concentration of 5.83 at.%. When an n-AZO film with a 5.83 at.% Al concentration was inserted between the ITO electrode and the active layer (PCDTBT:PC₇₀BM), the power conversion efficiency increased from 3.7 to 5.6%.</P>

The Genome Assembly and Annotation of the Southern Elephant Seal Mirounga leonina

Kim, Bo-Mi,Kim, Jeong-Hoon,Kim, Jin-Hyoung,Kang, Seunghyun,Jo, Euna,Lee, Jun Hyuck 극지연구소 2021 극지연구소 초록집 Vol.- No.-

Clinical Outcomes of Non-carbapenem Treatment for Urinary Tract Infections Caused by Extended-spectrum β-lactamase-producing Escherichia coli

Kim, Eunae,Ahn, Yo Han,Lee, Jung Won,Park, Eujin Korean Society of Pediatric Nephrology 2021 Childhood kidney diseases Vol.25 No.1

Purpose: The purpose of this study was to investigate the clinical outcomes of non-carbapenem treatment for urinary tract infections (UTIs) caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) in young children. Methods: We retrospectively reviewed the medical records of children under 2 years of age who were diagnosed and treated for UTIs caused by ESBL-producing E. coli from September 2014 to March 2020. Results: Forty-three children under 2 years of age were treated with non-carbapenem antimicrobials for UTIs caused by ESBL-producing E. coli without bloodstream infections. The overall clinical and microbiological success rates for empirical antimicrobial treatment were 90.7% and 97.7%. Three of the patients (7.0%) experienced a relapse of UTI within a month. An in vitro susceptibility test showed that two patients were sensitive and one was resistant to the antimicrobial treatments. Furthermore, there were no significant differences in the time to defervescence, clinical success, microbiological success, and relapse rate between the susceptible (n=13) and non-susceptible groups (n=30). Conclusion: In this study, the overall relapse rate of patients treated with non-carbapenem antimicrobials was 7.0%. The patients showed high success rates in the clinical and microbiological responses to the non-carbapenems regardless of the results of the in vitro antimicrobial susceptibility test. These results provide evidence that non-carbapenems may be viable alternative treatments for UTIs caused by ESBL-producing E. coli.

Epidermal Phytochrome B Inhibits Hypocotyl Negative Gravitropism Non-Cell-Autonomously

Kim, Jaewook,Song, Kijong,Park, Eunae,Kim, Keunhwa,Bae, Gabyong,Choi, Giltsu American Society of Plant Biologists 2016 The Plant cell Vol.28 No.11

<P>Seedling hypocotyls display negative gravitropism in the dark but agravitropism in the light. The Arabidopsis thaliana pif quadruple mutant (pifQ), which lacks four PHYTOCHROME-INTERACTING FACTORS (PIFs), is agravitropic in the dark. Endodermis-specific expression of PIF1 rescues gravitropism in pifQ mutant seedlings. Since phytochromes induce light responses by inhibiting PIFs and the COP1-SPA ubiquitin E3 ligase complex in the nucleus, we asked whether phyB can cell autonomously inhibit hypocotyl negative gravitropism in the endodermis. We found that while epidermis-specific expression of PHYB rescues hypocotyl negative gravitropism and all other phyB mutant phenotypes, endodermis-specific expression of PHYB does not. Epidermal phyB induces the phosphorylation and degradation of endodermal PIFs in response to red light. This induces a global gene expression pattern similar to that induced by red light treatment of seedlings expressing PHYB under the control of its own endogenous promoter. Our results imply that epidermal phyB generates an unidentified mobile signal that travels to the endodermis where it promotes PIF degradation and inhibits hypocotyl negative gravitropism.</P>

A novel discovery of a gaseous sH clathrate hydrate former

Kim, Eunae,Seo, Yongwon Elsevier 2019 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.359 No.-

<P><B>Abstract</B></P> <P>No gaseous guests have ever been discovered that can form sH clathrate hydrates. Herein, we show that a large molecular fluorinated gas (octafluorocyclobutane, c-C<SUB>4</SUB>F<SUB>8</SUB>), can stabilize the crystal structure of sH clathrate hydrate by occupying large (5<SUP>12</SUP>6<SUP>8</SUP>) cages. The inclusion of c-C<SUB>4</SUB>F<SUB>8</SUB> molecules in sH clathrate hydrate in the presence of CH<SUB>4</SUB> molecules was clearly demonstrated through three-phase (clathrate hydrate (H) – liquid water (L<SUB>W</SUB>) – vapor (V)) equilibria, powder X-ray diffraction (PXRD) and <SUP>13</SUP>C NMR spectroscopy. The discovery of a gaseous sH former, c-C<SUB>4</SUB>F<SUB>8</SUB>, contributes to broadening clathrate hydrate science and engineering by expanding target components for sH clathrate hydrates, and also offers excellent potential in various applications of sH clathrate hydrates, especially in clathrate hydrate-based gas separation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We examine the enclathration of c-C<SUB>4</SUB>F<SUB>8</SUB> in clathrate hydrate lattices. </LI> <LI> c-C<SUB>4</SUB>F<SUB>8</SUB> forms sH hydrate in presence of CH<SUB>4</SUB> as a help gas. </LI> <LI> We demonstrate c-C<SUB>4</SUB>F<SUB>8</SUB> is gaseous sH hydrate former. </LI> <LI> c-C<SUB>4</SUB>F<SUB>8</SUB> is enclathrated in large (5<SUP>12</SUP>6<SUP>8</SUP>) cages of sH hydrate. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

The Pentose Phosphate Pathway as a Potential Target for Cancer Therapy

( Eunae Sandra Cho ),( Yong Hoon Cha ),( Hyun Sil Kim ),( Nam Hee Kim ),( Jong In Yook ) 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.1

During cancer progression, cancer cells are repeatedly exposed to metabolic stress conditions in a resource-limited environment which they must escape. Increasing evidence indicates the importance of nicotinamide adenine dinucleotide phosphate (NADPH) homeostasis in the survival of cancer cells under metabolic stress conditions, such as metabolic resource limitation and therapeu-tic intervention. NADPH is essential for scavenging of reactive oxygen species (ROS) mainly derived from oxidative phosphoryla-tion required for ATP generation. Thus, metabolic reprogramming of NADPH homeostasis is an important step in cancer progres-sion as well as in combinational therapeutic approaches. In mammalian, the pentose phosphate pathway (PPP) and one-carbon metabolism are major sources of NADPH production. In this review, we focus on the importance of glucose flux control towards PPP regulated by oncogenic pathways and the potential therein for metabolic targeting as a cancer therapy. We also summarize the role of Snail (Snai1), an important regulator of the epithelial mesenchymal transition (EMT), in controlling glucose flux towards PPP and thus potentiating cancer cell survival under oxidative and metabolic stress.

Congenital fetal syphilis presenting with progression of ultrasound findings: A case report

( Eunae Jo ),( Hyung Eun Choi ),( Su Kyung Jung ),( Sina Jang ),( Jeong Ok Kim ),( Jeonghoon Lee ),( Ho Yeon Kim ),( Eun Seop Song ),( Byoung Ick Lee ),( Sung Ook Hwang ),( Soo Ran Choi ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

Congenital fetal syphilis can cause by spirochetes cross the placenta from about 14 weeks of gestation. The clinical manifestations of congenital fetal syphilis are the results of active infection and inflammation and they are influenced by gestational age, stage of maternal syphilis, maternal treatment, and immunological response of the fetus. Untreated syphilis in pregnancy leads to adverse outcomes, including still birth, prematurity, low birth weight, congenital disease among newborn babies. We have a case of active fetal syphilis infection and rapid course of disease in untreated maternal primary infection. A 16-year-old woman at 28+2 weeks of gestation was referred with maternal reactive of FTA-ABS Ig M and Ig G, and fetal hepatosplenomegaly, severe ascites, and thickened placenta on ultrasound scan. The woman had a history of painless small vulvar sore about one month ago, and a report of ultrasound scan showed fetal abdomen with scanty ascites at 27+3 weeks of gestation. Thus the woman had started treatment with benzathine penicillin 2.4 million units. However, the fetus resulted in intrauterine fetal death at 28+6 weeks gestation with severe fetal hydropse, ascites, and pleural effusion. Polymerase chain reaction of amniotic fluid for Treponema pallidum was positive and FTA-ABS Ig M in cord blood was reactive. It is important in all pregnant women during the first trimester of pregnancy should be screened serologically for syphilis.

Free-Energy Landscape of a Thrombin-Binding DNA Aptamer in Aqueous Environment

Kim, Eunae,Yang, Changwon,Pak, Youngshang American Chemical Society 2012 Journal of chemical theory and computation Vol.8 No.11

<P>Thrombin-binding aptamer (TBA-15) is a single-stranded 15-mer oligonucleotide that has a wide range of biomedical applications. In the presence of metal cations of proper sizes, this aptamer displays G-quadruplexes with a single cation enclosed at its central binding site when it is completely folded. To understand how this aptamer folds into its stable three-dimensional structure in the presence of K<SUP>+</SUP> ions, we carried out free-energy calculations using the state-of-art replica exchange molecular dynamics simulation (REMD) at the all-atom level. The resulting free energy map revealed that TBA-15 follows a two-state folding behavior with a substantially large folding barrier of 6 kcal/mol at ambient temperature. Our simulation showed that the intervening TGT-loop, which is located in the middle of the TBA-15 sequence, virtually remains intact regardless of folding and unfolding states. Furthermore, in the conserved TGT-loop structure, the base-pair stacking of G8 and T9 induces the native-like base orientations of G6 and G10 pertaining to the upper G-quadrant. This stacking interaction enhances the loop stability and reduces its dynamic fluctuations. Interestingly, for the G-stem to fold into its native state, the aggregation of the G8 and T9 residues in the TGT-loop is a key step for initiating the folding event of the G-stem by capturing a bulky cation.</P>