Meprobamate Chlorpromazine 및 電擊療法이 白鼠의 Estrous Cycle에 미치는 影響

羅鳳鎭,金恩洙,朱鍾球,金光日 大韓神經精神醫學會 1966 신경정신의학 Vol.5 No.1

The cyclical activity of the Sprague-Dawley strain rat was observed under the various conditions of administration of meprobamate, chlorpromazine and EST, using the vaginal smear technique. The experimental groups were as followings; GroupⅠ Normal control rats GroupⅡ Rats administered with meprobamate in doses of 60㎎/㎏/day GroupⅢ Rats administered with meprobamate in doses of 400㎎/㎏/day GroupⅣ Rats administered with meprobamate in doses of 400㎎/㎏/day and chlorpromazine, 20㎎/㎏/day GroupⅤ Rats administered with meprobamate in doses of 60㎎/㎏/day and chlorpromazine, 20㎎/㎏/day GroupⅥ Rats administered with meprobamate in doses of 60㎎/㎏/day and chlorpromazine, 5㎎/㎏/day GroupⅦ Rats administered with meprobamate in doses of 60-400㎎/㎏/day combined with EST. In Group Ⅱ and Ⅲ, the average length of the estrous cycle was 9.64 and 10.02 days, which revealed the significant prolongation in comparison with 4.9 days in the normals. Group Ⅳ and Ⅴ showed complete cessation of estrous state of the cycle. Group Ⅵ had irregular cyclical activity, showing 14.4 days interval of the cycle. Group Ⅶ had complete cessation of estrous stage of the cycle immediately after EST. It seems significant that meprobamate has an effect on the cyclical activity of the estrous cycle, which is increased if combined with chlorpromazine or EST.

A rare case of extensive papillary muscle calcification in senile cardiac calcification syndrome

김은진;송봉근;강민호;손형래;홍수민;박동원;허승혜;김계연;최석구 인제대학교 2011 仁濟醫學 Vol.32 No.-

광범위 유두근 석회화는 흔하지 앉으며, 원인과 임상적 중요성에 관한 문헌만 드물게 찾아볼 수 있다. 반면에 작은 석회 침착은 노인에서 흔하며 심장 첨부에 주로 나타난다. 유두근 석회화는 관상동맥질환, 확장성 심근병증, 승모관 질환, 고칼슘혈증, 그리고 말기신부전에서 인산칼슘의 중가와 연관성이 있다. 저자는 심장초옴파와 MDCT를 통해 진단된 전외측 유두근의 광범위 석회화의 드문 사례가 있어 보고한다.

생물막공법을 이용한 슬러지 저감에 관한 연구

정호진,구봉헌 대한상하수도학회 2003 상하수도학회지 Vol.17 No.2

Sludge reduction by biofilm processes in sewage treatment plants was studied. This resulted in a sharp reduction in the amount of sludge and improved water quality. This process maintains stable treatment independent of variations in temperature and pollution loading. Laboratory experiments and pilot plants tests were processed with raw waste water through the improved contact oxidation biofilm by biological endogenous phase. The obtained results were satisfactory. When the amount of sludge production, treatment efficiency, and economical efficiency are considered, 2.5 hours were the most efficient and economical. Also, stable and excellent treatment efficiency was maintained in spite of temperature change. The amount of sludge was sharply reduced by controling biological endogenous phase.

소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

담배 박각시나방 Manduca sexta의 계획된 세포죽음동안의 Proteasome의 변화

이구봉,우기민,조만희,이상한,장예진,김창세 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

모든 진핵세포의 주요 단백질 분해요소인 proteasome은 변성되거나 상해된 불필요한 단백질을 제거할 뿐만 아니라, 세포주기를 비롯한 다양한 대사과정들의 주요 효소들을 필요 시 적절히 분해함으로써 항상성을 유지시키는 필수적인 효소로 잘 알려져 있다. 또한 proteasome은 특정 포유동물 조직의 계획된 세포죽음(programmed cell death 또는 apoptosis)와 밀접하게 관련되어 있다고 보고된 바, 본 연구에서는 담배 박각시나방(Manduca sexta)의 변태과정에서 일어나는 마디간 근육(intersegmental muscle)의 급격한 파괴과정동안의 proteasome의 변화양상을 발현 및 단백질 분해활성을 중점으로 조사하였다. 정상근육과 파괴직전의 근육으로부터 proteasome을 분석한 결과 단백질 분해활성이 최소한 6배 이상 증가하였으며, proteasome의 주요 구성성분들의 발현 변화를 각각의 항체(antibody)를 이용하여 조사한 결과 세포죽음 직전에 급격히 증가함을 보였다. 이러한 구성성분들의 발현증가가 proteasome 복합체 형성을 통해 활성의 증가를 일으키는지 조사한 결과, 세포내에서 실질적으로 단백질 분해활성을 담당한다고 여겨지는 26S proteasome(2,000 kDa)의 위치에서 커다란 활성의 변화와 구성성분의 증가를 볼 수 있었다. 따라서, 담배 박각시나방의 계획된 세포죽음 현상은 특정 호르몬의 신호에 의해 proteasome의 발현이 급격히 증가하여 26S proteasome의 복합체 형태로 세포 내 대부분의 단백질들을 분해함으로써 30시간이내 근육조직을 완전히 소멸시키는 것을 알 수 있었다. 한편, proteasome을 구성하는 특정 구성성분의 과다 발현이 세포의 단백질 분해현상에 어떠한 영향을 미치는 지 조사하기 위하여 쥐의 fibroblast cell에 26S proteasome의 ATPase 구성성분인 m56 유전자를 삽입한 후 세포의 추출물로부터 proteasome의 양과 활성의 변화를 관찰하였다. Proteasome을 초원심분리로 추출하여 관찰한 결과, 담배 박각시나방의 경우와는 달리 유전자 삽입 세포는 모든 실험에서 정상 세포와 전혀 차이를 보이지 않았다. 이 결과로부터 세포의 생명 유지에 필수적이고 매우 다양한 기능을 가진 proteasome은 40여개의 구성성분들의 발현이 매우 정교하게 조절되고 있으며, 특정 세포죽음 자극(apoptotic signal)이 왔을 때 일련의 구성 성분들의 발현과 26S proteasome 복합체 형성이 증가함으로써 그 기능을 수행하는 것을 알 수 있으며, 보다 자세한 연구는 비정상적 단백질 분해나 세포죽음을 동반하는 여러 질환들의 원인과 현상을 규명할 수 있으며, 또한 그 활성을 조절함으로써 궁극적인 치료의 방향도 제시 가능할 것으로 기대된다.

C3H/Hej 마우스에서 모발성장에 대한 생약 추출물의 효과

구성근, 주진호, 허진주, 미가, 강봉수, 김준형, 남상윤, 윤영원, 이범준, 김종수 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.3

본문참고

경쟁불안, 스포츠자신감 및 자기효능감이 귀인형태에 미치는 영향

최영옥,구봉진 한국체육대학교 체육과학연구소 2000 韓國體育大學校附屬 體育科學硏究所論文集 Vol.19 No.1

The present study was designed to examine the effect of competitive anxiety, sport-confidence, and self-efficacy on attributional patterns across male and female table tennis competitors in athletic settings. 82 table tennis competitors reported their competitive anxiety, sport-confidence, self-efficacy and attributional patterns on Competitive State Anxiety-2, Sport Confidence Inventory, Self-Efficacy Inventory and Attributional Patterns Inventory. The implications of the study are as follows: 1.State sport confidence had positive impact on ability attribution in winners, and trait sport confidence had negative effect on ability attribution in losers. 2.Outcome self-efficacy had positive impact on effort attribution in winners, and trait sport confidence, performance self-efficacy had negative effect on effort attribution in losers. 3.Cognitive state anxiety had positive impact on luck attribution in winners, and trait sport confidence had negative effect on luck attribution in losers. But somatic state anxiety had positive influence on luck attribution in losers. 4.Trait sport confidence, outcome self-efficacy had positive impact on task difficulty attribution in winners, and state sport confidence negative effect on task difficulty attribution in losers.

도시노인의 신체적 여가활동참여와 사회심리적 건강과의 관계

최영옥,구봉진,임나리 한국체육대학교 체육과학연구소 2000 韓國體育大學校附屬 體育科學硏究所論文集 Vol.19 No.1

This paper is to clarify the relationship between participation in physical leisure activities, past exercise experience and social psychological health in the urban aged. For this purpose, through multi-stage random sampling, a total of 328 (176 males; 152 females) were selected among the old in their 65 to 75 in Seoul. As for the measuring instrument, SAS (Self-rating Anxiety Scale), developed by Zung(1971), and the questionnaires, translated by Jang Dong-san et al. (1988), were used for the measurement of anxiety; SDS (Self-rating Depression Scale), developed by Zung and Durham (1965) and translated by Jang Dong-san (1988), was used for the measurement of melancholy; the questionnaire, translated and standardized by Baek He-won (1993) from SES (Self-Esteem scale) of Rosenberg (1965), was used for the measurement of self-esteem; and LSI-W Life Satisfaction Index-Well-Being), standardized by Bigot(1974), was used for life satisfaction. Through analysis of the relationship between participation in physical leisure activities, past exercise experience and social psychological health in the urban aged, the conclusion was drawn as follows: There are differences in social psychological health according to physical leisure activities. Both males and females showed statistically significant differences in self-esteem, life satisfaction, melancholy and anxiety according to participation in physical leisure activities. That is, as compared with the group of non-participation in exercises, the groups of regular participation and participation in exercises related with sports showed higher self-esteem and life satisfaction, and lower melancholy and anxiety

마우스에서 자외선조사에 의한 피부주름형성에 대한 생약추출물의 억제효과

구성근, 배부희, 허진주, 미가, 강봉수, 김준형, 남상윤, 윤영원, 김종수, 이범준 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.3

본문참고

대학생들의 자살관련행동에 관한 연구

노명선,전홍진,이해우,이효정,한성구,함봉진 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.1

Objectives : This study evaluated the prevalence of suicide-related behaviors (suicidal ideation, plan and attempt) and the status of depression, anxiety and function in college students. Methods : A cross-sectional study was completed to a sample of 880 students with the self-administered form of Korean version of the Mini International Neuropsychiathc Interview (K-MINI), Beck Depression Inventory (BDI) and Beck Anxiety Inventory (BAI). Results : Estimated lifetime prevalence of suicidal ideation was 39.2%, that of suicidal plan was 4.7% and that of suicidal attempt was 3.0%. Students with any suicide-related behaviors showed higher lifetime prevalence of major and minor depression, higher BDI and BAI scores, and poor intemersonal relationship, adaptation to college life and physical health compared to the students with no suicide-related behaviors. The average points of grades during their college life were not significantly different according to kinds of suicide-related behaviors they expehenced (F=0.39, P=0.82). 96% of suicidal attempters did not use mental health services. Conclusion : Results suggest that a high proportion of college students experience suicide-related behaviors. The Stu-dents who had experienced suicide-related behaviors showed higher depression and anxiety and poorer function compared to students with no suicide-related behaviors. Most of suicidal attempters, however, did not use mental health service Establishing campus mental health system is necessary to early detect mental health problems and to prevent suicide of college students.