Numerical Implementation for a 2-D Thermal Inhomogeneity Through the Dynamical Probe Method

Global Science Press 2010 Journal of computational mathematics Vol.28 No.1

The characteristic of Cu2ZnSnS4 thin film solar cells prepared by sputtering CuSn and CuZn alloy targets

Yilei Lu,Shurong Wang,Xun Ma,Xin Xu,Shuai Yang,Yaobin Li,Zhen Tang 한국물리학회 2018 Current Applied Physics Vol.18 No.12

Recent study shows that the main reason for limiting CZTS device performance lies in the low open circuit voltage, and crucial factor that could affect the Voc is secondary phases like ZnS existing in absorber layer and its interfaces. In this work, the Cu2ZnSnS4 thin film solar cells were prepared by sputtering CuSn and CuZn alloy targets. Through tuning the Zn/Sn ratios of the CZTS thin films, the crystal structure, morphology, chemical composition and phase purity of CZTS thin films were characterized by X-Ray Diffraction (XRD), scanning electron microscopy (SEM) equipped with an energy dispersive spectrometer (EDS) and Raman spectroscopy. The statistics data show that the CZTS solar cell with a ratio of Zn/Sn=1.2 have the best power convention efficiency of 5.07%. After HCl etching process, the CZTS thin film solar cell with the highest efficiency 5.41% was obtained, which demonstrated that CZTS film solar cells with high efficiency could be developed by sputtering CuSn and CuZn alloy targets.

A Primary Study for Bioprinting of Human Liver

( Yilei Mao ),( Huayu Yang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

This study is aim to construct human liver tissue structure using 3D biological printing technology. We used the characteristics of stem cell differentiation and cell 3D printing technology to investigate the progress of stem cell differentiation, 3D printing process and training components of the environment. The biological properties of cell-cell and cell matrix in natural liver tissue were successful reproduction with realizing human liver cells mature and maintaining long-term function. In addition to this, bile duct network was widely distributed in the cellular structure, improving the three-dimensional structure of the material exchange ability, and ultimately the formation of long-term survival in vitro with drug metabolizing function of human liver tissue. The liver function of 3D printing human liver tissue in vivo was evaluated by using Fah-/- model mice with hereditary tyrosine degeneration. The results showed that the 3D printing liver tissue significantly prolonged the survival time and improve the liver function in mice. It can be concluded that the human liver tissue model based on 3D printing technology has the primary liver function of liver, which provides a new tool for drug research and disease mechanism research.

Engineering a High-Affinity PD-1 Peptide for Optimized Immune Cell-Mediated Tumor Therapy

Yilei Chen,Hongxing Huang,Yin Liu,Zhanghao Wang,Lili Wang,Quanxiao Wang,Yan Zhang,Hua Wang 대한암학회 2022 Cancer Research and Treatment Vol.54 No.2

Purpose The purpose of this study was to optimize a peptide (nABP284) that binds to programmed cell death protein 1 (PD-1) by a computer-based protocol in order to increase its affinity. Then, this study aimed to determine the inhibitory effects of this peptide on cancer immune escape by coculturing improving cytokine-induced killer (ICIK) cells with cancer cells. Materials and Methods nABP284 that binds to PD-1 was identified by phage display technology in our previous study. AutoDock and PyMOL were used to optimize the sequence of nABP284 to design a new peptide (nABPD1). Immunofluorescence was used to demonstrate that the peptides bound to PD-1. Surface plasmon resonance was used to measure the binding affinity of the peptides. The blocking effect of the peptides on PD-1 was evaluated by a neutralization experiment with human recombinant programmed death-ligand 1 (PD-L1) protein. The inhibition of activated lymphocytes by cancer cells was simulated by coculturing of human acute T lymphocytic leukemia cells (Jurkat T cells) with human tongue squamous cell carcinoma cells (Cal27 cells). The anticancer activities were determined by coculturing ICIK cells with Cal27 cells in vitro. Results A high-affinity peptide (nABPD1, KD=11.9 nM) for PD-1 was obtained by optimizing the nABP284 peptide (KD=11.8 μM). nABPD1 showed better efficacy than nABP284 in terms of increasing the secretion of interkeulin-2 by Jurkat T cells and enhancing the in vitro antitumor activity of ICIK cells. Conclusion nABPD1 possesses higher affinity for PD-1 than nABP284, which significantly enhances its ability to block the PD-1/PD-L1 interaction and to increase ICIK cell-mediated antitumor activity by armoring ICIK cells. PurposeThe purpose of this study was to optimize a peptide (nABP284) that binds to programmed cell death protein 1 (PD-1) by a computer-based protocol in order to increase its affinity. Then, this study aimed to determine the inhibitory effects of this peptide on cancer immune escape by coculturing improving cytokine-induced killer (ICIK) cells with cancer cells.Materials and MethodsnABP284 that binds to PD-1 was identified by phage display technology in our previous study. AutoDock and PyMOL were used to optimize the sequence of nABP284 to design a new peptide (nABPD1). Immunofluorescence was used to demonstrate that the peptides bound to PD-1. Surface plasmon resonance was used to measure the binding affinity of the peptides. The blocking effect of the peptides on PD-1 was evaluated by a neutralization experiment with human recombinant programmed death-ligand 1 (PD-L1) protein. The inhibition of activated lymphocytes by cancer cells was simulated by coculturing of human acute T lymphocytic leukemia cells (Jurkat T cells) with human tongue squamous cell carcinoma cells (Cal27 cells). The anticancer activities were determined by coculturing ICIK cells with Cal27 cells <i>in vitro</i>.ResultsA high-affinity peptide (nABPD1, K_D=11.9 nM) for PD-1 was obtained by optimizing the nABP284 peptide (K_D=11.8 μM). nABPD1 showed better efficacy than nABP284 in terms of increasing the secretion of interkeulin-2 by Jurkat T cells and enhancing the <i>in vitro</i> antitumor activity of ICIK cells.ConclusionnABPD1 possesses higher affinity for PD-1 than nABP284, which significantly enhances its ability to block the PD-1/PD-L1 interaction and to increase ICIK cell-mediated antitumor activity by armoring ICIK cells.

A novel energy regeneration system for emulsion pump tests

Li Yilei,Zhu Zhencai,Chen Guoan,Cao Guohua 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.4

A novel energy regeneration system based on cylinders and a rectifier valve for emulsion pump tests is presented and studied. The overall structure and working principles of this system are introduced. Both simulation and experiments are carried out to investigate the energy regeneration feasibility and capability of this novel system. The simulation and experimental results validate that this system is able to save energy and satisfy the test requirement. The energy recovery coefficient and overall energy regeneration coefficient of the test bench are 0.785 and 0.214, respectively. Measures to improve these two coefficients are also given accordingly after analysis of power loss. This novel system brings a new method of energy regeneration for emulsion pump tests.

인구규모로 본 베이징 도시화 진행과정 실태

장예뢰(Zhang, yilei),김한수(Kim, Han-soo) 한국주거학회 2022 한국주거학회 학술대회논문집 Vol.34 No.1

인구규모로 본 베이징 도시화 진행과정 실태

장예뢰(Zhang, yilei),김한수(Kim, Han-soo) 한국주거학회 2022 한국주거학회 학술대회논문집 Vol.33 No.2

영적 멘토링과 일의 의미: 소명의식의 매개효과

박준형,Yilei Chen 리더십학회 2023 리더십연구 Vol.14 No.4

일 의미감의 중요성을 고려할 때, 조직은 어떻게 구성원의 일 의미감을 강화할 수 있는지를 깊이이해해야 한다. 본 연구에서 일 의미감을 높이기 위해, 일의 영적 차원, 특히 영적 멘토링에 집중하였다. 영적 멘토링의 영향력을 이해하는 것이 중요함에도, 이 분야의 연구는 아직 제한적이다. 따라서, 본 연구에서 영적 멘토링이 멘티의 일 의미감을 어떻게 증가시키는 지에 대한 메커니즘을 이해하고자 하였다. 표본 데이터는 중국의 다양한 조직에서 근무하는 직원들로부터 수집되었고, 152명의 참가자로부터의 얻은 데이터를 바탕으로, 구조방정식 모형을 사용하여 분석하였다. 분석 결과, 멘티의 소명의식이영적 멘토링과 일 의미감 사이의 관계를 매개한다는 것을 확인하였다. 본 연구를 통해 일터에서의 멘토링과 영성을 연결시켜 새로운 관점을 제시함으로써 관련 문헌에 중요한 기여를 하였다. 또한 멘터의영적 멘토링과 멘티들이 느끼는 일의 의미 사이의 관계를 조사함으로써, 멘토링 분야를 영적인 영역을넓혔고, 영적 멘토링과 멘티의 일의 의미 사이의 연결 메커니즘을 밝혔다. 이를 바탕으로 이 연구에서의 의미, 제한점 그리고, 실무를 위한 제언을 함께 논의하였다. Given the significance of work meaningfulness, it is crucial for organizations to understand how to foster and enhance it. This paper focuses on one particular avenue to bolster work meaningfulness: the spiritual dimension of work, specifically spiritual mentoring. Despite the importance of understanding the effects of spiritual mentoring on work meaningfulness, research in this area remains limited. Thus, we attempt to provide an understanding of the spiritual mentoring process that increases the work meaningfulness of protégés. Our sample data was collected from employees across a diverse range of organizations in China. We received complete responses from 152 participants, and the data was analyzed using the structural equation modeling method. Our results confirmed that a protégé's sense of calling mediated the relationship between the mentor’s spiritual mentoring and work meaningfulness. We make several important contributions to the relevant literature by introducing a new perspective, drawing a connection between mentoring and spirituality within the work environment. We investigate the relationship between spiritual mentoring and the meaningfulness of work for protégés, thus broadening the scope of mentoring literature to include spiritual mentoring. Additionally, this study delves into the mechanism linking spiritual mentoring and the work meaningfulness of protégés.

Structure evolution in carbon molecular sieve membranes derived from binaphthol-6FDA polyimide and their gas separation performance

Guoxiong Deng,Yilei Wang,Xueping Zong,Jiangzhou Luo,Xuezhen Wang,Chunxue Zhang,Song Xue 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.94 No.-

Here we reported a causal relationship between the molecular structure of binaphthol-based polyimideprecursor and the pore-size distribution of the derived carbon membrane. The binaphthol-basedpolyamide acid is synthesized from 2,20-diol-1,10-binaphthyl-6,60-diamine and 4,40-(hexafluoroisopro-pylidene)diphthalic anhydride (6FDA). Then, an azeotropic imidiaztion method was used to synthesizethe polyimide with naphthol groups (XS1). When the imidization is carried out by using acetic anhydride,the polyimide with acetyl groups (XS4) is achieved. The CMS membranes prepared by pyrolyzing XS1 andXS4 at 500, 550, and 600 ℃ are named using the temperature as the suffix, such as XS1-500. Their poreevolution has been investigated using TGA, FTIR, XRD, and Raman measurements. The trimodal pore-sizedistribution is in the carbon molecular sieve (CMS) membranes derived from XS4 and the CMS onesderived from XS1 exhibit a bimodal pore structure. Among them, XS4-500 exhibits the highest gaspermeabilities of 3332 barrer for CO2, 773 barrer for O2, and 119 barrer for N2. XS1-500 only affords theCO2, O2, and N2 permeabilities of 1086, 230, and 30.2 barrer. The esterification of naphthol not justdisturbs the hydrogen bonds between polyimide chains but also affects the pore generation of thederived CMS membranes. Our work provides an effective way to enhance the gas permeability of a CMSmembrane derived from the binaphthol-based polyimide.

CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming

Xiangyu Lu,Yilei Wu,Rui Cao,Xiaojiong Yu,Jun Gong 한국통합생물학회 2022 Animal cells and systems Vol.26 No.4

Pancreatic stellate cells (PSCs) are the primary cell components of pancreatic cancer (PC) and are involved in tumor growth, metastasis and resistance. However, the role and the mechanism of PSCs in gemcitabine (GEM) resistance to PC still need more investigation. We found that CXCL12 mRNA and secreted CXCL12 protein were higher in PSCs after GEM treatment. The conditioned medium (CM) from GEM-treated PSCs reduced the GEM sensitivity of PC cells. Blocking of CXCL12 in CM by anti-CXCL12 antibody partly restored the GEM sensitivity of PC cells. Blocking of CXCL12 decreased glucose consumption, lactate production, ECAR, and glycolysis-related gene expression in PC cells. The PI3K/AKT/mTOR pathway was activated by the binding of CXCL12 and CXCR4. Moreover, CXCR4 mRNA and protein expressions in PC cells were increased after GEM treatment. Our results indicated the cross-talk between PSCs and PC cells during GEM chemotherapy. CXCL12 secreted by PSCs reduces GEM sensitivity of PC cells by binding to CXCR4 and activating PI3K/AKT/mTOR-glycolysis pathway in PC. Our findings would lay the foundation for solving GEM resistance in PC.