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Qun-Ying Jin,Hua-Zheng Peng,Er-Pei Lin,Nan Li,Dan-Ni Huang,Yan-Li Xu,Xi-Qi Hua,Kui-Hong Wang,Tang-Jun Zhu 한국식물학회 2016 Journal of Plant Biology Vol.59 No.4
As one of the largest members of Poaceae family, bamboo is a very important agricultural plant in the world. The development of bamboo shoot is very special and particularly significant to bamboo production. Understanding the developmental differences between bamboo shoot and rhizome shoot is extremely valuable for us to further elucidate the mechanism of bamboo shoot formation since both bamboo shoot and rhizome shoot develop directly from rhizome bud underground. In this paper, miRNA chips with 413 miRNA probes were used to compare miRNA expressions between bamboo shoot and rhizome shoot. The experiment revealed 64 bamboo shoot upregulated and 56 rhizome shoot up-regulated miRNAs which were classified into four major categories according to deep sequencing based target prediction. Meristem and morphological development related miRNAs were most important in bamboo shoot, especially miR171 and miR156 members. While in rhizome shoot the mainstream of miRNA expressions was metabolism and nutrition related ones, especially miR395 members. The meristem and morphological development related miRNAs in bamboo shoot showed some embryonic characteristics and suggested the participation of several phytohormones like gibberellin, cytokinin and auxin, which were absent in those miRNAs of rhizome shoot. Further qRT-PCR detections of 21 up-regulated miRNAs in bamboo seedlings indicated that 12 ones were regulated to varying degrees by some environmental factors. Among them, rhizome shoot upregulated osa-miR395b was the most environment-sensitive miRNA, particularly to dehydration. And the bamboo shoot up-regulated osa-miR399j proved uniquely and strongly induced by phosphor. The existence of multiple regulation sites from same miRNA suggested the probability of crosstalks among meristem development, metabolism and stress response during bamboo shoot and rhizome shoot development.
Ying-Ya Cao,Zhong-Han Wang,Qian-Cheng Xu,Qun Chen,Zhen Wang,Wei-Hua Lu 대한생리학회-대한약리학회 2021 The Korean Journal of Physiology & Pharmacology Vol.25 No.4
The intestinal barrier function disrupted in sepsis, while little is known about the variation in different phases of sepsis. In this study, mouse models of sepsis were established by caecal ligation and puncture (CLP). The H&E staining of sections and serum diamine oxidase concentration were evaluated at different timepoint af-ter CLP. TUNEL assay and EdU staining were performed to evaluate the apoptosis and proliferation of intestinal epithelium. Relative protein expression was assessed by Western blotting and serum concentrations of pro-inflammatory cytokines was mea-sured by ELISA. The disruption of intestinal barrier worsened in the first 24 h after the onset of sepsis and gradually recovered over the next 24 h. The percentage of apop-totic cell increased in the first 24 h and dropped at 48 h, accompanied with the prolif-erative rate of intestinal epithelium inhibited in the first 6 h and regained in the later period. Furthermore, the activity of nuclear factor kappa B (NF-κB) presented similar trend with the intestinal barrier function, shared positive correction with apoptosis of intestinal epithelium. These findings reveal the conversion process of intestinal barrier function in sepsis and this process is closely correlated with the activity of NF-κB signaling.
Curcumin Alleviates Dystrophic Muscle Pathology in mdx Mice
Ying Pan,Chen Chen,Yue Shen,Chun-Hua Zhu,Gang Wang,Xiao-Chun Wang,Hua-Qun Chen,Min-Sheng Zhu 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.4
Abnormal activation of nuclear factor kappa B (NF-κB) probably plays an important role in the pathogenesis of Duchenne’s muscular dystrophy (DMD). In this report, we evaluated the efficacy of curcumin, a potent NF-κB inhibitor, in mdx mice, a mouse model of DMD. We found that it improved sarcolemmic integrity and enhanced muscle strength after intraperitoneal (i.p.) injection. Histological analysis revealed that the structural defects of myofibrils were reduced, and biochemical analysis showed that creatine kinase (CK) activity was decreased. We also found that levels of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and inducible nitric oxide synthase (iNOS) in the mdx mice were decreased by curcumin administration. EMSA analysis showed that NF-κB activity was also inhibited. We thus conclude that curcumin is effective in the therapy of muscular dystrophy in mdx mice, and that the mechanism may involve inhibition of NF-κB activity. Since curcumin is a nontoxic compound derived from plants, we propose that it may be useful for DMD therapy.
Yang, Xin-Ying,Cai, Sheng-Xin,Zhang, Wen-Ji,Tang, Xue-Lian,Shin, Hye-Young,Lee, Joo-Young,Gu, Qian-Qun,Park, Hyun Pharmaceutical Society of Japan 2008 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.31 No.12
<P>Semi-vioxanthin isolated from marine-derived fungus was assessed for immunoregulatory activity in mouse RAW264.7 macrophages. In the present study, the facilitative effects of semi-vioxanthin on tumor necrosis factor-α (TNF-α) and its mRNA expression and on expression of the co-stimulatory molecules, cluster of differentiation (CD) 80, CD86 and major histocompatibility complex class II (MHC II), as well as the molecular mechanism underlying the immunologic enhancement properties of semi-vioxanthin were studied. Our results clearly indicated that semi-vioxanthin treatment resulted in the degradation of IκBα, which led to the activation and nuclear translocation of the p65 subunit of nuclear factor-κB (NF-κB), as determined by immunoblotting, immunofluorescence and electrophoretic mobility shift assays (EMSA). Moreover, TNF-α production was prevented by NF-κB and mitogen-activated protein kinase (MAPK) inhibitors. Inhibition of NF-κB and extracellular signal regulated kinases (ERK1/2) activity by specific inhibitors blunted the effect of semi-vioxanthin on the up-regulation of CD80, CD86 and MHCII expression, but neither p38 MAPK nor c-Jun N-terminal kinase (JNK) inhibitor had this effect. Thus, we demonstrate that semi-vioxanthin regulates TNF-α production through NF-κB and MAPK signaling pathways. Activation of NF-κB and ERK1/2 were necessary for CD80, CD86 and MHCII expression induced by semi-vioxanthin. These data suggest that semi-vioxanthin has immunoregulatory effects.</P>
Junhui Zhang,Qun Chao,Bing Xu,Min Pan,Yuan Chen,Qiannan Wang,Ying Li 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.7
When manufacturing axial piston pumps, mass difference of piston-slipper assembly is inevitable because of manufacturing precision limits. Small mass difference may not cause problems when the pump operates at low speeds, while it cannot be ignored at high speeds. One problem related to high speed is the cylinder block tilt resulting from the inertial effect of piston-slipper assembly. Recently, the speed of electro-hydrostatic actuator (EHA) pump in aircraft can reach more than 10,000 rpm. Therefore, mass difference of pistonslipper assembly should be taken into account in future EHA pump design. The main purpose of this paper is to investigate the effect of the mass difference of piston-slipper assembly on the cylinder block tilt in a high-speed EHA pump. A detailed set of relevant equations is developed to establish the relationship between the mass difference of piston-slipper assembly and cylinder block tilting moment. It is found that a tighter control over the mass difference of piston-slipper assembly should be guaranteed when it comes to high-speed EHA pumps.
Huang Zhi-Qun,Ye Jing,Liu Jing,Sun Li-Ying,Ong Hsiao Hui,Wei Yong-Hao,Fu Shu-Cai,Hu Xiao-Xun,Xu Yu,Wang De-Yun 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.4
Purpose: The abnormal expression of tight junction (TJ) plays a vital role in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). However, there is no appropriate tool to distinguish and diagnose epithelial barrier defects in clinical practice. This study aimed to evaluate the predictive value of claudin-3 for epithelial barrier dysfunction in CRSwNP. Methods: In this study, TJ protein levels were evaluated by real-time quantitative polymerase chain reaction, immunofluorescent, and immunohistochemistry staining in control subjects and CRSwNP patients. The receiver operating characteristic (ROC) curve was created to assess the predictive value of TJ breakdown in clinical outcomes. In vitro, human nasal epithelial cells were cultured at the air-liquid interface to analyze the transepithelial electrical resistance (TER) level. Results: The expression levels of occludin, tricellulin, claudin-3, and claudin-10 were decreased (all P < 0.05), and those of claudin-1 was increased (P < 0.05) in CRSwNP patients as compared to healthy subjects. Additionally, claudin-3 and occludin levels were negatively correlated with the computed tomography score in CRSwNP (all P < 0.05), and the ROC curve indicated that the claudin-3 level had the most predictive accuracy in evaluating epithelial barrier disruption (area under the curve = 0.791, P < 0.001). Finally, the time-series analysis showed the highest correlation coefficient between TER and claudin-3 (cross-correlation function = 0.75). Conclusion: In this study, we suggest that claudin-3 could be a valuable biomarker for predicting nasal epithelial barrier defects and disease severity in CRSwNP.
Performance and applications of flow-guided sieve trays for distillation of highly viscous mixtures
Hui Liu,Qun Shen Li,Chun Ying Song,Hai Long Wu,Yi Qiu Qian 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.6
With a particular focus on the distillation of highly viscous or self-polymerized mixtures, this study reports the hydrodynamic and mass transfer performance of two flow-guided sieve trays, including their pressure drop, entrainment, weeping and tray efficiency, obtained experimentally with an air-water/oxygen system in a Φ600 mm plexiglass column. The results show that the 8 mm hole flow-guided sieve tray tested shows better characteristics than the 7 mm flow-guided hole tray in terms of pressure drop and mass transfer. Then we present practical industrial examples of applications of the flow guided trays for distillation of viscous mixtures, i.e., the separation of vinyl acetate (VAC) from a polyvinyl acetate (PVAC) polymer solution with dynamic viscosity μ=50,000 mPas, the separation of highly unsaturated C5 mixtures by extractive distillation, and the distillation of thick, condensed and highly viscous fermentation mixtures made from fermented mash. It is demonstrated that flow-guided sieve trays with relatively large holes are an excellent candidate for distillation of mixtures with suspended solids, or concentrated/self-polymerized polymer solutions.