Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis

Li, Juan,Bian, Wei-He,Wan, Juan,Zhou, Jing,Lin, Yan,Wang, Ji-Rong,Wang, Zhao-Xia,Shen, Qun,Wang, Ke-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Curdione, one of the major components of Curcuma zedoaria, has been reported to possess various biological activities. It thus might be a candidate anti-flammatory and cancer chemopreventive agent. However, the precise molecular mechanisms of action of curdione on cancer cells are still unclear. In this study, we investigated the effect of curdione on breast cancer. Materials and Methods: Xenograft nude mice were used to detect the effect of curdione on breast cancer in vivo; we also tested the effect of curdione on breast cancer in vitro by MTT, Flow cytometry, JC-I assay, and western blot. Results: Firstly, we found that curdione significantly suppressed tumor growth in a xenograft nude mouse breast tumor model in a dose-dependent manner. In addition, curdione treatment inhibited cell proliferation and induced cell apoptosis. Moreover, after curdione treatment, increase of impaired mitochondrial membrane potential occurred in a concentration dependent manner. Furthermore, the expression of apoptosis-related proteins including cleaved caspase-3, caspase-9 and Bax was increased in curdione treatment groups, while the expression of the anti-apoptotic Bcl-2 was decreased. Inhibitors of caspase-3 were used to confirm that curdione induced apoptosis. Conclusions: Overall, our observations first suggested that curdione inhibited the proliferation of breast cancer cells by inducing apoptosis. These results might provide some molecular basis for the anti-cancer activity of curdione.

Multi-objective Optimization for Time-Open Lambert Rendezvous Between Non-coplanar Orbits

Guan-Qun Wu,Li-Guo Tan,Xin Li,Shen-Min Song 한국항공우주학회 2020 International Journal of Aeronautical and Space Sc Vol.21 No.2

The problem of time-open Lambert multi-objective optimal rendezvous between non-coplanar orbits is studied in the paper. Based on the Lambert’s theorem, the descriptions of time-open Lambert rendezvous subjected to two-impulse and three-impulse are presented, and a universally applicable method for the solution of the transfer orbits is given. To deal with the multi-objective optimization subject to fuel consumption and flight time for rendezvous, an improved NSGA-II algorithm with a better performance is provided. Via the global optimization, Pareto set can be obtained by the improved NSGA-II for the multi-objective optimal rendezvous considering multi-constraints. Numerical simulations are conducted to validate the feasibility of the improved multi-objective optimization algorithm, and the results of the two-impulse and three-impulse multi-objective optimal rendezvous are shown.

Efficient and eco-friendly plasma preparation and reaction mechanism of low surface-energetic core-shell CaTiO3

Xiaolei Zheng,Qun Wang,Xiaoqiang Li,Changkuan Zheng,Chenjie Shen 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.5

Surface modification of powder materials has always been a key issue that must be faced to improve their applicationproperties and application value. Herein, an efficient and eco-friendly CH4/CF4 plasma method is used for surface modificationof materials. The surface modification mechanism of fluorocarbon plasma under different gas flow ratios was detailedinvestigated by combining the kinetic analysis. The findings demonstrate that fluorocarbon plasma exhibits differentmodification mechanisms under different modification conditions. The physicochemical properties of the material surface canbe easily controlled by adjusting the ratio of fluorocarbon plasma. Functional CaTiO3 particles with core-shell structure weresuccessfully prepared when the gas flow rate ratio R=3. The modified surface exhibited extreme hydrophobicity and the lowestsurface energy. This strong application of fluorocarbon plasma technology in material surface modification offers a newavenue to prepare environmentally friendly and functional powders.

Performance and applications of flow-guided sieve trays for distillation of highly viscous mixtures

Hui Liu,Qun Shen Li,Chun Ying Song,Hai Long Wu,Yi Qiu Qian 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.6

With a particular focus on the distillation of highly viscous or self-polymerized mixtures, this study reports the hydrodynamic and mass transfer performance of two flow-guided sieve trays, including their pressure drop, entrainment, weeping and tray efficiency, obtained experimentally with an air-water/oxygen system in a Φ600 mm plexiglass column. The results show that the 8 mm hole flow-guided sieve tray tested shows better characteristics than the 7 mm flow-guided hole tray in terms of pressure drop and mass transfer. Then we present practical industrial examples of applications of the flow guided trays for distillation of viscous mixtures, i.e., the separation of vinyl acetate (VAC) from a polyvinyl acetate (PVAC) polymer solution with dynamic viscosity μ=50,000 mPas, the separation of highly unsaturated C5 mixtures by extractive distillation, and the distillation of thick, condensed and highly viscous fermentation mixtures made from fermented mash. It is demonstrated that flow-guided sieve trays with relatively large holes are an excellent candidate for distillation of mixtures with suspended solids, or concentrated/self-polymerized polymer solutions.

Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)

He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4

Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.

Cinobufacin Suppresses Cell Proliferation via miR-494 in BGC-823 Gastric Cancer Cells

Zhou, Rong-Ping,Chen, Gang,Shen, Zhi-Li,Pan, Li-Qun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Cinobufacin is used clinically to treat patients with many solid malignant tumors. However, the mechanisms underlying action remain to be detailed. Our study focused on miRNAs involved in cinobufacin inhibition of GC cell proliferation. miRNA microarray analysis and real time PCR identified miR-494 as a significant cinobufacin-associated miRNA. In vivo, ectopic expression of miR-494 inhibited the proliferation and induced apoptosis of BGC-823 cells on CCK-8 and flow cytometry analysis. Further study verified BAG-1 (anti-apoptosis gene) to bea target of miR-494 by luciferase reporter assay and Western blotting. In summary, our study demonstrated that cinobufacin may inhibit the proliferation and promote the apoptosis of BGC-823 cells. Cinobufacin-associated miR-494 may indirectly be involved in cell proliferation and apoptosis by targeting BAG-1, pointing to use as a potential molecular target of cinobufacin in gastric cancer therapy.

Methylated Alteration of SHP1 Complements Mutation of JAK2 Tyrosine Kinase in Patients with Myeloproliferative Neoplasm

Yang, Jun-Jun,Chen, Hui,Zheng, Xiao-Qun,Li, Hai-Ying,Wu, Jian-Bo,Tang, Li-Yuan,Gao, Shen-Meng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

SHP1 negatively regulates the Janus kinase 2/signal transducer and activator of transcription (JAK2/STAT) signaling pathway, which is constitutively activated in myeloproliferative neoplasms (MPNs) and leukemia. Promoter hypermethylation resulting in epigenetic inactivation of SHP1 has been reported in myelomas, leukemias and other cancers. However, whether SHP1 hypermethylation occurs in MPNs, especially in Chinese patients, has remained unclear. Here, we report that aberrant hypermethylation of SHP1 was observed in several leukemic cell lines and bone marrow mononuclear cells from MPN patients. About 51 of 118 (43.2%) MPN patients including 23 of 50 (46%) polycythaemia vera patients, 20 of 50 (40%) essential thrombocythaemia and 8 of 18 (44.4%) idiopathic myelofibrosis showed hypermethylation by methylation-specific polymerase chain reaction. However, SHP1 methylation was not measured in 20 healthy volunteers. Hypermethylation of SHP1 was found in MPN patients with both positive (34/81, 42%) and negative (17/37, 45.9%) JAK2V617F mutation. The levels of SHP1 mRNA were significantly lower in hypermethylated samples than unmethylated samples, suggesting SHP1 may be epigenetically inactivated in MPN patients. Furthermore, treatment with 5-aza-2'-deoxycytidine (AZA) in K562 cells showing hypermethylation of SHP1 led to progressive demethylation of SHP1, with consequently increased reexpression of SHP1. Meanwhile, phosphorylated JAK2 and STAT3 were progressively reduced. Finally, AZA increased the expression of SHP1 in primary MPN cells with hypermethylation of SHP1. Therefore, our data suggest that epigenetic inactivation of SHP1 contributes to the constitutive activation of JAK2/STAT signaling. Restoration of SHP1 expression by AZA may contribute to clinical treatment for MPN patients.

Phase II Study on Voriconazole for Treatment of Chinese Patients with Malignant Hematological Disorders and Invasive Aspergillosis

Zhang, Xue-Zhong,Huang, Xin-En,Xu, Yan-Li,Zhang, Xiu-Qun,Su, Ai-ling,Shen, Zheng-Shan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To investigate the efficacy and safety of voriconazole in treating Chinese patients with hematological malignancies and invasive aspergillosis. Methods: From March 2007 to April 2012, patients with diagnoses confirmed by CT, GM test and/or PCR assays, were recruited into this study. Aspergillosis of all patients were treated with voriconazole 6 mg/kg intravenous infusion (iv) every 12 h for 1 day, followed by 4 mg/kg IV every 12 h for 10-15 days; Then, switch to oral administration that was 200mg every 12h for 4-12 weeks. Efficacy and safety were evaluated according to Practice Guideline of Infectious Diseases Society of America. Results: The overall response rate of 38 patients after voriconazole treatment was 81.6%. The median time to pyretolysis was 4.5 days. Treatment related side effects were mild and found in only 15.8% of cases. No treatment related deaths occurred. Conclusions: Voriconazole can considered to be a safe and effective front-line therapy to treat patients with hematological malignancies and invasive aspergillosis. Alternatively it could be used as a remedial treatment when other antifungal therapies are ineffective.

Influence of Curcumin on HOTAIR-Mediated Migration of Human Renal Cell Carcinoma Cells

Pei, Chang-Song,Wu, Hong-Yan,Fan, Fan-Tian,Wu, Yi,Shen, Cun-Si,Pan, Li-Qun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

Background: This study investigated the influence of curcumin on HOX transcript antisense RNA (HOTAIR)-mediated migration of cultured renal cell carcinoma (RCC) cells. Materials and Methods: Five RCC cell lines (769-P, 769-P-vector, 769-P-HOTAIR, 786-0, and Kert-3 ) were maintained in vitro. The expression of HOTAIR mRNA was determined by quantitative real-time PCR and cell migration was measured by transwell migration assay. The effects of different concentrations of curcumin (0 to $80{\mu}mol/L$) on cell proliferation was determined by the CCK-8 assay and influence of non-toxic levels (0 to $10{\mu}M$) on the migration of RCC cells was also determined. Results: Comparison of the 5 cell lines indicated a correlation between HOTAIR mRNA expression and cell migration. In particular, the migration of 769-P-HOTAIR cells was significantly higher than that of 769-P-vector cells. Curcumin at $2.5-10{\mu}M$ had no evident toxicity against RCC cells, but inhibited cell migration in a concentration-dependent manner. Conclusions: HOTAIR expression is correlated with the migration of RCC cells, and HOTAIR may be involved in the curcumin-induced inhibition of RCC metastasis.