http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Nai‑Yong Liu,Ze‑Ran Bao,Jing Li,Xin‑Yu Ao,Jia‑Ying Zhu,Yu‑Hui Chen 한국유전학회 2017 Genes & Genomics Vol.39 No.5
The fungal genus Trichoderma has been extensively studied due to its role in the mycoparasitism, and thus developed as biocontrol agent against various plant pathogens. Although the mycoparasitic processes of several Trichoderma species have already been well understood, the information about the mycoparasitic mechanisms of Trichoderma strains resulted from different growth conditions or interacting with different phytopathogens is still limited. In this study, we utilized transcriptome sequencing to identify the differentially expressed genes (DEGs) at 0, 24, 72 and 120 h from T. atroviride strain SS003, growing on an induced-medium with cell walls of Pinus armandii pathogen Cronartium ribicola (CRCW). In total, 86,155,316 reads were obtained with 43,077,658 clean reads. Further, 10,422 genes were identified from four transcriptomes and accounted for 93.89% of annotated genes in T. atroviride IMI 206040 genome, reflecting high-quality sequencing and assembly. In each pairwise comparison, a large number of DEGs were identified with different numbers of genes for up- and down-regulation, respectively. In the presence of CRCW, expression of two main glycoside hydrolase gene families (i.e. chitinase and glucosidase) was induced. Most of 14 secreted enzymes by quantitative real time PCR (qPCR) analysis exhibited a consistent expression pattern with that by RNA-Seq data. This comparative study leads to the identification of phase-specific genes in the interactions of T. atroviride SS003 with C. ribicola, and provides potential molecular targets for improved biocontrol strategies.
Chemosensory genes from Pachypeltis micranthus, a natural enemy of the climbing hemp vine
Nai-Yong Liu,Jia-Ying Zhu,Mei Ji,Bin Yang,Sang-Zi Ze 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
The plant bug, Pachypeltis micranthus (Hemiptera: Miridae), is a natural enemy of the invasive alien weed Mikania micrantha with a widespread distribution in South China as well as other countries. The interactions of P. micranthus and its host M. micrantha, associated with a linkage of host recognition, are of great importance for its survival and reproduction. The identification of olfactory-related genes is undoubtedly the initially key step, to uncover how P. micranthus perceives and recognizes the specific host plant M. micrantha. Here, we constructed an antennal full length cDNA library from P. micranthus antennae. By sequencing, a total of 13 transcripts encoding chemosensory-related genes were identified, comprising nine odorant-binding proteins (OBPs), three chemosensory proteins (CSPs) and one odorant receptor (OR). All identified OBPs and CSPs shared classic characteristics of conserved cysteines and a signal peptide. Expression profiles by quantitative real time PCR (qPCR) revealed that as many as 12/13 chemosensory genes were expressed predominantly in the antennae at a sex-biased pattern, strongly linking to their specific olfactory functions. Notably, expression of all these genes was also differentially detected in legs of both sexes with an exception of PmicOBP7, indicative of their functional differentiation between female and male adults. Further, molecular docking of PmicOBP6 and CSP1 to behaviorally active compounds provided the potentially key residues for ligand-binding, which deserves further studies.
Nai-Yong Liu,Guo-XingWu,Sang-Zi Ze,Bin Yang,Jia-Ying Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
The genital systems of insects are undoubtedly of great key for the success of mating and population reproduction. In the meantime,morphological and ultrastructural characteristics of the reproductive systems also provide valuable evidences for the studies of taxonomy, reproductive biology and evolutionary biology.More previously, the reproductive systemof female Nasonia vitripennis was already described. To complement the information of reproductive systems from this species, the male reproductive system was here dissected and characterized by light microscopy and transmission electron microscopy. Results showed that the system follows the pattern of most Hymenoptera species: paired testes with one follicle, deferent ducts, seminal vesicles, accessory glands and a fused ejaculatory duct ultimately connected to the external aedeagus. Histologically, the testes, seminal vesicles and accessory glands are all composed of three portions, all of which include epithelial and luminal regions. In addition, both lipidic inclusions and nuclei are presented in the three organs with variable sizes and shapes. Spermatozoa are observed in the testes and seminal vesicles, and also vary considerably in size and shape, suggesting different phases of spermatogenesis.Mitochondria are abundant in seminal vesicles and accessory glands. Notably, membranous inclusions and Golgi complexes are found only in seminal vesicles, whereas secretory granules are presented only in accessory glands, being indicative of organ or age specificity. Together, this study complements the information of the reproductive systems from N. vitripennis, and provides an extensive resource for taxonomy, reproductive biology and evolutionary biology.
Molecular and enzymatic characterization of acid phosphatase from venom of Scleroderma guani
Nai-Yong Liu,Xiao-Hong Fan,Zhi-Quan Zhang,Guo-Xing Wu,Jia-Ying Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
Acid phosphatase (ACPase) is a common component in venom of parasitoids. Although extensive researches regarding this enzyme have been conducted in many other organisms, its characteristics as a venomous enzyme are still sparsely known. In this study, we aimed to reveal the gene expression patterns, and structural and biochemical properties of an ACPase from the venom of Scleroderma guani. The cloned open reading frame of venomous ACPase gene of S. guani was 1218 bp encoding 406 deduced amino acids, shared 40% and 41% identities to ACPases from venoms of Apis mellifera and Pteromalus puparum, respectively. The structural analysis of this ACPase implied common functions and differences to the honeybee venom ACPase. qPCR analysis showed that this gene was abundantly expressed in the venom apparatus, and most highly expressed in the adult stage after one and three days emergence. Activity assay using p-nitrophenyl phosphate as the substrate revealed that the optimal pH and temperature for this venomous enzyme was 4.8 and 45 °C, respectively. NaF is an effective inhibitor for it. The results will enrich our knowledge for the ACPase as toxin, which may contribute to further uncovering its role involved in parasitism.
영동선 동백산-도계간 터널내 반사법 탄성파탐사 적용사례
김용일(Yong-Il Kim),윤영훈(Young-Hoon Yoon),조상국(Sang-Kook Cho),양종화(Jong-hwa Yang),김장수(Jang-Soo Kim),이내용(Nai-Yong Lee) 한국철도학회 2002 한국철도학회 학술발표대회논문집 Vol.- No.-
Seismic Reflection Methods(TSP, HSP) have been applied in the junction between 2nd Adit and Main Tunnel (Solan Tunnel) of Youngdiong Railroad(Mt. Dongbaek~Dokye). In this paper, methods and case study will be introduced to predict discontinuties in the tunnel before excavation by the Seismic Reflection Methods(TSP, HSP)and secure construction stability of the tunnel in blasting and excavation.
대전 동서관통도로 Front-Jacking공법 시공사례
김용일(Kim Yong-Il),황낙연(Hwang Nak-Yeon),차종휘(Cha Jong-Whi),장성욱(Jang Sung-Wook),이내용(Lee Nai-Yong) 한국철도학회 2005 한국철도학회 학술발표대회논문집 Vol.- No.-
The crossing construction under railroad have two methods which are cut and cover and trenchless method. First, cut and cover method is an extremely limited method concerning non-running time. Whereas, trenchless method is free from restriction such as train speed and running time, and has the strong points of safe and rapid construction. Front Jacking method, one of the trenchless methods, is frequently applied recently due to its stability during construction and vantage of assuring schedule reliability. The procedure is that after minimizing interlocking friction with structure and earth pressure due to jacking the small steel tube, pulling the precast box manufactured at the field in the ground using PC strand and hydraulic Jack. This method is able to be applied regardless of section size and length of box and condition of soil. And that is also pro-environmental. This paper presents the case of Daejeon E. W. perforate Road Project applied with the Front Jacking method.
Nai-Bin Chen,Bo Qiu,Jun Zhang,Meng-Yun Qiang,Yu-Jia Zhu,Bin Wang,Jin-Yu Guo,Ling-Zhi Cai,Shao-Min Huang,Meng-Zhong Liu,Qun Li,Yong-Hong Hu,Qi-Wen Li,Hui Liu 대한암학회 2020 Cancer Research and Treatment Vol.52 No.1
Purpose The purpose of this study was to compare the survival and toxicities in cervical esophageal squamous cell carcinoma (CESCC) treated by concurrent chemoradiothrapy with either three-dimensional conformal radiotherapy (3D-CRT) or intensity-modulated radiotherapy (IMRT) techniques. Materials and Methods A total of 112 consecutive CESCC patients were retrospectively reviewed. 3D-CRT and IMRT groups had been analyzed by propensity score matching method, with sex, age, Karnofsky performance status, induction chemotherapy, and tumor stage well matched. The Kaplan- Meier method and Cox proportional hazards model were used for overall survival (OS) and progression-free survival (PFS). Toxicities were compared between two groups by Fisher exact test. Results With a median follow-up time of 34.9 months, the 3-year OS (p=0.927) and PFS (p=0.859) rate was 49.6% and 45.8% in 3D-CRT group, compared with 54.4% and 42.8% in IMRT group. The rates of grade ! 3 esophagitis, grade ! 2 pneumonitis, esophageal stricture, and hemorrhage were comparable between two groups, while the rate of tracheostomy dependence was much higher in IMRT group than 3D-CRT group (14.3% vs.1.8%, p=0.032). Radiotherapy technique (hazard ratio [HR], 0.09; 95% confidence interval [CI], 0.01 to 0.79) and pretreatment hoarseness (HR, 0.12; 95% CI 0.02 to 0.70) were independently prognostic of tracheostomy dependence. Conclusion No survival benefits had been observed while comparing IMRT versus 3D-CRT in CESCC patients. IMRT with fraction dose escalation and pretreatment hoarseness were considered to be associated with a higher risk for tracheostomy dependence. Radiation dose escalation beyond 60 Gy should be taken into account carefully when using IMRT with hypofractionated regimen.