Identification and expression profiling of six chemosensory protein genes in the beet armyworm, Spodoptera exigua

Jia-Ying Zhu,Sang-Zi Ze,Bin Yang 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.1

Chemosensory proteins (CSPs) are believed to be important in the detection of semiochemicals, and have been reported to be involved in development, molting, leg regeneration and phase transition. In the current study, six CSP genes were identified in the beet armyworm Spodoptera exigua, namely SexiCSP1-6, from the full length cDNA library.Multiple alignment and phylogenetic analysis indicated that theywere very diverse as seen in CSPs of other insect species. Expression patterns determined by quantitative real-time PCR (qPCR) revealed that they were highly expressed in antenna. SexiCSP1-3, SexiCSP5 and SexiCSP6 were male antenna-biased, while SexiCSP4 was female-biased in the antenna. In addition to antenna, they were expressed at high levels in legs and wings, suggesting potential roles in contact chemoreception and non-olfactory roles. Compared with the expression in antenna, legs and wings, only SexiCSP1 showed relatively high expression in heads, thoraxes and abdomen. These results extended olfactory gene resource in the beet armyworm and provided basis for further gaining a better understanding of CSPs roles in this pest.

Upregulation of coleoptericin transcription in Tenebrio molitor parasitized by Scleroderma guani

Jia-Ying Zhu,Guo-Xing Wub,Zhong Zhang 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.3

Three antibacterial peptide genes, coleoptericins A, B and C, were identified from the yellow meal worm beetle, Tenebriomolitor. Their cDNA sequences encoded 13.01, 13.13 and 14.16 kDa peptides, respectively. Their deduced amino acid sequences showed 16–34% identity with other coleopteran coleoptericins, containing over 20% glycine. These genes were essentially expressed in fat body and hemocytes, and at late-instar larval and pupal stages. Their transcriptswere upregulated by bacterial challenge and Scleroderma guani parasitization. It suggests that triggered coleoptericin transcription is applied to prevent host potential infection during parasitization.

The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in <i>Arabidopsis</i>

Zhu, Jia-Ying,Li, Yuyao,Cao, Dong-Mei,Yang, Hongjuan,Oh, Eunkyoo,Bi, Yang,Zhu, Shengwei,Wang, Zhi-Yong Elsevier 2017 Molecular cell Vol.66 No.5

<P><B>Summary</B></P> <P>The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In <I>Arabidopsis</I>, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated brassinosteroid (BR) signaling. Here we identify KIB1 as an F-box E3 ubiquitin ligase that promotes the degradation of BIN2 while blocking its substrate access. Loss-of-function mutations of KIB1 and its homologs abolished BR-induced BIN2 degradation and caused severe BR-insensitive phenotypes. KIB1 directly interacted with BIN2 in a BR-dependent manner and promoted BIN2 ubiquitination in vitro. Expression of an F-box-truncated KIB1 caused BIN2 accumulation but dephosphorylation of its substrate BZR1 and activation of BR responses because KIB1 blocked BIN2 binding to BZR1. Our study demonstrates that KIB1 plays an essential role in BR signaling by inhibiting BIN2 through dual mechanisms of blocking substrate access and promoting degradation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> KIB1 is an essential positive regulator in brassinosteroid signaling </LI> <LI> KIB1 mediates BR-induced ubiquitination and degradation of GSK3 kinase BIN2 </LI> <LI> KIB1 binding to BIN2 prevents BIN2-substrate interaction and promotes BIN2 degradation </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

New hydroperoxylated and 20,24-epoxylated dammarane triterpenes from the rot roots of Panax notoginseng

Jia-Huan Shang,Wen-Jie Sun,Hong-Tao Zhu,Dong Wang,Chong-Ren Yang,Ying-Jun Zhang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3

Background: Root rot is a serious destructive disease of Panax notoginseng, a famous cultivated araliaceousherb called Sanqi or Tianqi in Southwest China. Methods: The chemical substances of Sanqi rot roots were explored by chromatographic techniques. MS,1D/2D-NMR, and single crystal X-ray diffraction were applied to determine the structures. Murinemacrophage RAW264.7 and five human cancer cell lines were used separately for evaluating the antiinflammatoryand cytotoxic activities. Results and Conclusion: Thirty dammarane-type triterpenes and saponins were isolated from the rot rootsof P. notoginseng. Among them, seven triterpenes, namely, 20(S)-dammar-25-ene-24(S)-hydroperoxyl-3b,6a,12b,20-tetrol (1), 20(S)-dammar-3-oxo-23-ene-25-hydroperoxyl-6a,12b,20-triol (2), 20(S)-dammar-12-oxo-23-ene-25-hydroperoxyl-3b,6a,20-triol (3), 20(S)-dammar-3-oxo-23-ene-25-hydroperoxyl-12b,20-diol (4), 20(S),24(R)-epoxy-3,4-seco-dammar-25-hydroxy-12-one-3-oic acid (5), 20(S),24(R)-epoxy-3,4-seco-dammar-25-hydroxy-12-one-3-oic acid methyl ester (6), and 6a-hydroxy-22,23,24,25,26,27-hexanordammar-3,12,20-trione (7), are new compounds. In addition, 12 known ones (12e16 and 19e25)were reported in Sanqi for the first time. The new Compound 1 showed comparable antiinflammatoryactivity on inhibition of NO production to the positive control, whereas the known compounds 9, 12, 13,and 16 displayed moderate cytotoxicities against five human cancer cell lines. The results will providescientific basis for understanding the chemical constituents of Sanqi rot roots and new candidates forsearching antiinflammatory and antitumor agents.

Sensing Performance of Efficient Cyclostationary Detector with Multiple Antennas in Multipath Fading and Lognormal Shadowing Environments

Zhu, Ying,Liu, Jia,Feng, Zhiyong,Zhang, Ping The Korea Institute of Information and Commucation 2014 Journal of communications and networks Vol.16 No.2

Spectrum sensing is a key technical challenge for cognitive radio (CR). It is well known that multicycle cyclostationarity (MC) detection is a powerful method for spectrum sensing. However, a conventional MC detector is difficult to implement because of its high computational complexity. This paper considers reducing computational complexity by simplifying the test statistic of a conventional MC detector. On the basis of this simplification process, an improved MC detector is proposed. Compared with the conventional detector, the proposed detector has low-computational complexity and high-accuracy sensing performance. Subsequently, the sensing performance is further investigated for the cases of Rayleigh, Nakagami-m, Rician, and Rayleigh fading and lognormal shadowing channels. Furthermore, square-law combining (SLC) is introduced to improve the detection capability in fading and shadowing environments. The corresponding closed-form expressions of average detection probability are derived for each case by the moment generation function (MGF) and contour integral approaches. Finally, illustrative and analytical results show the efficiency and reliability of the proposed detector and the improvement in sensing performance by SLC in multipath fading and lognormal shadowing environments.

Analysis of Improved Cyclostationary Spectrum Sensing with SLC Diversity over Composite Multipath Fading-Lognormal Shadowing Channels

( Ying Zhu ),( Jia Liu ),( Zhiyong Feng ),( Ping Zhang ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.3

Spectrum sensing is a key technical challenge for cognitive radio (CR). It is well known that multi-cycle cyclostationarity (MC) detector is a powerful method for spectrum sensing. However, conventional MC detector is difficult to implement due to its high computational complexity. This paper pays attention to the fact that the computation complexity can be reduced by simplifying the test statistic of conventional MC detector. Based on this simplification process, an improved MC detector is proposed. Compared with the conventional one, the proposed detector has the low-computational complexity and sufficient-accuracy on sensing performance. Subsequently, the sensing performances are further investigated for the cases of Rayleigh, Nakagami-m, Rician, composite Rayleigh fading-lognormal shadowing and composite Nakagami fading-lognormal shadowing channels, respectively. Furthermore, the square-law combining (SLC) is introduced to improve the detection capability over fading-shadowing channels. The corresponding closed-form expressions of average detection probability are derived for each case by the moment generation function (MGF) approach. Finally, illustrative and analytical results show that the efficiency and reliability of proposed detector and the improvement on sensing performance by SLC over composite fading-shadowing channels.

Optimal Energy-Efficient Power Allocation and Outage Performance Analysis for Cognitive Multi-Antenna Relay Network Using Physical-Layer Network Coding

( Jia Liu ),( Ying Zhu ),( Guixia Kang ),( Yifan Zhang ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.12

In this paper, we investigate power allocation scheme and outage performance for a physical-layer network coding (PNC) relay based secondary user (SU) communication in cognitive multi-antenna relay networks (CMRNs), in which two secondary transceivers exchange their information via a multi-antenna relay using PNC protocol. We propose an optimal energy-efficient power allocation (OE-PA) scheme to minimize total energy consumption per bit under the sum rate constraint and interference power threshold (IPT) constraints. A closed-form solution for optimal allocation of transmit power among the SU nodes, as well as the outage probability of the cognitive relay system, are then derived analytically and confirmed by numerical results. Numerical simulations demonstrate the PNC protocol has superiority in energy efficiency performance over conventional direct transmission protocol and Four-Time-Slot (4TS) Decode-and-Forward (DF) relay protocol, and the proposed system has the optimal outage performance when the relay is located at the center of two secondary transceivers.

Sensing Performance of Efficient Cyclostationary Detector with Multiple Antennas in Multipath Fading and Lognormal Shadowing Environments

Ying Zhu,Jia Liu,Zhiyong Feng,Ping Zhang 한국통신학회 2014 Journal of communications and networks Vol.16 No.2

Spectrum sensing is a key technical challenge for cognitiveradio (CR). It is well known that multicycle cyclostationarity(MC) detection is a powerful method for spectrum sensing. However, a conventional MC detector is difficult to implementbecause of its high computational complexity. This paper considersreducing computational complexity by simplifying the teststatistic of a conventional MC detector. On the basis of this simplificationprocess, an improved MC detector is proposed. Comparedwith the conventional detector, the proposed detector haslow-computational complexity and high-accuracy sensing performance. Subsequently, the sensing performance is further investigatedfor the cases of Rayleigh, Nakagami-m, Rician, and Rayleighfading and lognormal shadowing channels. Furthermore, squarelawcombining (SLC) is introduced to improve the detection capabilityin fading and shadowing environments. The correspondingclosed-form expressions of average detection probability are derivedfor each case by the moment generation function (MGF) andcontour integral approaches. Finally, illustrative and analytical resultsshow the efficiency and reliability of the proposed detectorand the improvement in sensing performance by SLC in multipathfading and lognormal shadowing environments.

Risk Factors for Early Recurrence of HBV-related Hepatocellular Carcinoma Meeting Milan Criteria after Curative Resection

Zhu, Wen-Jiang,Huang, Chu-Ying,Li, Chuan,Peng, Wei,Wen, Tian-Fu,Yan, Lv-Nan,Li, Bo,Wang, Wen-Tao,Xu, Ming-Qing,Yang, Jia-Yin,Jiang, Li Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: The prognosis of patients with hepatocellular carcinoma (HCC) after curative resection varies greatly. Few studies had investigated the risk factors for early recurrence (recurrence-free time ${\leq}$ 1 year) of hepatitis B virus (HBV)-related HCCs meeting Milan criteria. Methods: A retrospective analysis was performed on the 224 patients with HCC meeting Milan criteria who underwent curative liver resection in our center between February 2007 and March 2012. The overall survival (OS) rate, recurrence-free survival (RFS) rate and risk factors for early recurrence were analyzed. Results: After a median follow-up of 33.3 months, HCC reoccurred in 105 of 224 patients and 32 died during the period. The 1-, 3- and 5-year OS rates were 97.3%, 81.6% and 75.6% respectively, and the 1-, 3- and 5-year RFS rates were 73.2%, 53.7% and 41.6%. Cox regression showed alpha-fetoprotein (AFP) > 800 ng/ml (HR 2.538, 95% CI 1.464-4.401, P=0.001), multiple tumors (HR 2.286, 95% CI 1.123-4.246, P=0.009) and microvascular invasion (HR 2.518, 95% CI 1.475-4.298, P=0.001) to be associated with early recurrence (recurrence-free time ${\leq}$ 1-year) of HCC meeting Milan criteria. Conclusions: AFP > 800 ng/ml, multiple tumors and microvascular invasion are independent risk factors affecting early postoperative recurrence of HCC. In addition resection appears capable of replacing liver transplantation in some situations with safety and a better outcome.

Optimal Power Allocation and Outage Analysis for Cognitive MIMO Full Duplex Relay Network Based on Orthogonal Space-Time Block Codes

( Jia Liu ),( Guixia Kang ),( Ying Zhu ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.3

This paper investigates the power allocation and outage performance of MIMO full-duplex relaying (MFDR), based on orthogonal space-time block codes (OSTBC), in cognitive radio systems. OSTBC transmission is used as a simple means to achieve multi-antenna diversity gain. Cognitive MFDR systems not only have the advantage of increasing spectral efficiency through spectrum sharing, but they can also extend coverage through the use of relays. In cognitive MFDR systems, the primary user experiences interference from the secondary source and relay simultaneously, owing to full duplexing. It is therefore necessary to optimize the transmission powers at the secondary source and relay. In this paper, we propose an optimal power allocation (OPA) scheme based on minimizing the outage probability in cognitive MFDR systems. We also analyse the outage probability of the secondary user in noise-limited and interference-limited environments in Nakagami-m fading channels. Simulation results show that the proposed schemes achieve performance improvements in terms of reducing outage probability.