Expression of yeast Hem1 gene controlled by Arabidopsis HemA1 promoter improves salt tolerance in Arabidopsis plants

( Zhi Ping Zhang ),( Quan Hong Yao ),( Liang Ju Wang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.5

5-Aminolevulinate (ALA) is well-known as an essential biosynthetic precursor of all tetrapyrrole compounds, which has been suggested to improve plant salt tolerance by exogenous application. In this work, the gene encoding aminolevulinate synthase (ALA-S) in yeast (Saccharomyces cerevisiae Hem1) was introduced into the genome of Arabidopsis controlled by the Arabidopsis thaliana HemA1 gene promoter. All transgenic lines were able to transcribe the YHem1 gene, especially under light condition. The chimeric protein (YHem1-EGFP) was found co-localizing with the mitochondria in onion epidermal cells. The transgenic Arabidopsis plants could synthesize more endogenous ALA with higher levels of metabolites including chlorophyll and heme. When the T2 homozygous seeds were cultured under NaCl stress, their germination and seedling growth were much better than the wild type. Therefore, introduction of ALA-S gene led to higher level of ALA metabolism with more salt tolerance in higher plants. [BMB reports 2010; 43(5): 330-336]

Expression profiles of microRNAs in skeletal muscle of sheep by deep sequencing

Zhi-Jin Liu,Cun-Yuan Li,Xiao-Yue Li,Yang Yao,Wei Ni,Xiang-Yu Zhang,Yang Cao,Wureli Hazi,Dawei Wang,Renzhe Quan,Shuting Yu,Yuyu Wu,Songmin Niu,Yulong Cui,Yaseen Khan,Shengwei Hu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.6

Objective: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative real-time polymerase chain reaction. To further investigate the function of miRNAs, candidate targeted genes were enriched for analysis using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment. Results: The results showed total of 1,086 known miRNAs and 40 new candidate miRNAs were detected in prenatal and postnatal skeletal muscle of sheep. In addition, 345 miRNAs (151 up-regulated, 94 down-regulated) were differentially expressed. Moreover, miRanda software was performed to predict targeted genes of miRNAs, resulting in a total of 2,833 predicted targets, especially miR-381 which targeted multiple muscle-related mRNAs. Furthermore, GO and KEGG pathway analysis confirmed that targeted genes of miRNAs were involved in development of skeletal muscles. Conclusion: This study supplements the miRNA database of sheep, which provides valuable information for further study of the biological function of miRNAs in sheep skeletal muscle.

Psychosocial Response and Symptom Burden for Male Smokers with Lung Cancer

Niu, Zhi-Min,Liang, Chun-Shui,Yu, Min,Wang, Yong-Sheng,Yu, Hai-Xia,Zhang, Qiong-Wen,Wei, Yu-Quan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Purpose: Cigarette smoking causes many kinds of cancer, and it is more closely related with lung cancer, rather than other cancers. Smoking is the leading cause of lung cancer and ninety percent of the smokers are male in China, but there is little published data concerning the psychological responses in the male smokers with lung cancer and its influence on the symptom burden. The aim of the study was to verify the hypothesis that male smokers with lung cancer have more positive attitude and less symptom burden, comparing to male non-smokers. Methods: A total of 194 men with cancer in West China Hospital, Sichuan, China, were assessed by self-administered questionnaire. Psychological response was measured by the Chinese version of Mini-Mental Adjustment to Cancer scale (Mini-MAC), and symptom burden was measured by the physical symptom distress scale from the Rotterdam Symptom Checklist (RSCL). Results: We found that smokers with lung cancer got higher scores in positive attitude and a smaller symptom burden than non-smokers. Patients with education lower than high school got higher scores of positive attitude compared to college graduate patients (p=0.038). Smokers with lung cancer who knew the potential carcinogenicity of cigarette showed less negative emotions (p=0.011). The psychological response was not affected by age, clinical stage, cell type, smoking duration and amount. Conclusions: Male smokers with lung cancer have a more positive attitude and fewer symptoms, comparing to male non-smokers. Appropriate psychological intervention for non-smokers with lung cancer deserves more attention.

Dynamic Control Allocation Algorithm for a Class of Distributed Control Systems

Dengfeng Zhang,Shen-Peng Zhang,Zhi-Quan Wang,Baochun Lu 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.2

The control allocation algorithm is developed for a class of distributed control systems with hierarchical structure and similar models of the subsystems, to solve the real-time cooperative control taking the dynamics and possible variations of subsystems into consideration. The augmented state-space model of the subsystems is first established for the control allocation. Based on robust control theory, the constrained and convex optimization is then developed with LMIs form to design the gain matrix of the dynamic control allocation. Furthermore, the closed-loop stability of the distributed control system is analyzed on the basis of stable upper-level main control law. Finally, simulations on the four-corner leveling control system of an advanced hydraulic press demonstrate the effectiveness of the proposed control allocation algorithm.

Analysis of Germplasm in American Black Walnut by RAPD Markers

Xiang Shen,Zhi quan. Mao,Ji xiang. Zhang,Lei Wang,Jing Zhang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Increased accumulation of anthocyanins in transgenic potato tubers by overexpressing the 3GT gene

Qing Wei,Qing Yang,Quan-Yi Wang,Zhi-Hang Feng,Bing Wang,Yun-Feng Zhang 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.1

In order to explore a biotechnological method for improving potato tuber color and creating plants with increased anthocyanin contents, a potato UDP-glucose: flavonoid-3-O-glucosyltransferase (3GT) gene was inserted behind the GBSSI promoter of pBin19, and this construct was introduced into Solanum tuberosum L. cultivar De´sire´e plants by Agrobacterium-mediated transformation. Six independent transgenic lines overexpressing the 3GT gene were identified by PCR and Southern blot analysis from 18 kanamycin-resistant plants. Due to the expression of 3GT gene, the tuber color and the anthocyanin content were enhanced noticeably in the transgenic plants compared to the wild-type control plants. This result suggests that the 3GT gene can potentially be used to improve potato color and enhance levels of antioxidants in the diet.

Time Trends of Esophageal Cancer Mortality in Linzhou City During the Period 1988-2010 and a Bayesian Approach Projection for 2020

Liu, Shu-Zheng,Zhang, Fang,Quan, Pei-Liang,Lu, Jian-Bang,Liu, Zhi-Cai,Sun, Xi-Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

In recent decades, decreasing trends in esophageal cancer mortality have been observed across China. We here describe esophageal cancer mortality trends in Linzhou city, a high-incidence region of esophageal cancer in China, during 1988-2010 and make a esophageal cancer mortality projection in the period 2011-2020 using a Bayesian approach. Age standardized mortality rates were estimated by direct standardization to the World population structure in 1985. A Bayesian age-period-cohort (BAPC) analysis was carried out in order to investigate the effect of the age, period and birth cohort on esophageal cancer mortality in Linzhou during 1988-2010 and to estimate future trends for the period 2011-2020. Age-adjusted rates for men and women decreased from 1988 to 2005 and changed little thereafter. Risk increased from 30 years of age until the very elderly. Period effects showed little variation in risk throughout 1988-2010. In contrast, a cohort effect showed risk decreased greatly in later cohorts. Forecasting, based on BAPC modeling, resulted in a increasing burden of mortality and a decreasing age standardized mortality rate of esophageal cancer in Linzhou city. The decrease of esophageal cancer mortality risk since the 1930 cohort could be attributable to the improvements of socialeconomic environment and lifestyle. The standardized mortality rates of esophageal cancer should decrease continually. The effect of aging on the population could explain the increase in esophageal mortality projected for 2020.

Endophytic Fungal strains Specifically Modified the Biochemical Status of Grape Cells

Li-Hua Huang,Ming-Zhi Yang,Xiu-Jin Ao,An-Yun Ren,Ming-Quan Yuan,Han-Bo Zhang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.4

Previously, specific interactions in morphologywere observed between grape cells and endophytic fungalstrains during a dual culture experiment. However, thebiochemical impacts of these fungal endophytes on grapecells is also expected due to their potential application in grapequality management. After assessed multiple physiochemicaltraits to those grape cells which have co-cultured with differentendophytic fungal strains in this study, and found the presenceof fungal endophytes obviously triggered ROS stress responsesin grape cells, and the biochemical status in grape cells weredifferentially modified by different fungal strains. In thosetested endophytic fungal strains, RH37 (Epicoccum sp.),RH6 (Alternaria sp.), RH32 (Alternaria sp.) and RH34(Trichothecium sp.) conferred greater metabolic impacts ongrape cells. And soluble protein (SPr), total flavonoids (TF),total phenols (TPh) and malondialdehyde (MDA) on the otherhand, were sensitive biochemical parameters which can beinfluenced in greater ranges than other detected parameters. Most interestedly, fungal endophytes shaped metabolitespatterns in grape cells during the dual culture appeared fungalgenus/species/strain-specificity. The work confirmed thesignificance of fungal endophytes in grape metabolic regulationand elucidated the possibility to purposely manage grapequality using tool of fungal endophytes.

Molecular and enzymatic characterization of acid phosphatase from venom of Scleroderma guani

Nai-Yong Liu,Xiao-Hong Fan,Zhi-Quan Zhang,Guo-Xing Wu,Jia-Ying Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

Acid phosphatase (ACPase) is a common component in venom of parasitoids. Although extensive researches regarding this enzyme have been conducted in many other organisms, its characteristics as a venomous enzyme are still sparsely known. In this study, we aimed to reveal the gene expression patterns, and structural and biochemical properties of an ACPase from the venom of Scleroderma guani. The cloned open reading frame of venomous ACPase gene of S. guani was 1218 bp encoding 406 deduced amino acids, shared 40% and 41% identities to ACPases from venoms of Apis mellifera and Pteromalus puparum, respectively. The structural analysis of this ACPase implied common functions and differences to the honeybee venom ACPase. qPCR analysis showed that this gene was abundantly expressed in the venom apparatus, and most highly expressed in the adult stage after one and three days emergence. Activity assay using p-nitrophenyl phosphate as the substrate revealed that the optimal pH and temperature for this venomous enzyme was 4.8 and 45 °C, respectively. NaF is an effective inhibitor for it. The results will enrich our knowledge for the ACPase as toxin, which may contribute to further uncovering its role involved in parasitism.

Design and Implementation on Spatial Science and Technology Information Database of CSI

Chen, Xiu Wan,Deng, Zheng Quan,Lu, Zhi Gao,Ma, Jia,Lin, Jia Yuan,Zhang, Wen Jiang,Luo, Tianfu,Liu, Baofu 대한원격탐사학회 2000 International Symposium on Remote Sensing Vol.16 No.1

Remote Sensing technology, which is characterized by producing imagery an multi-platform, different temporal and spatial resolution, has greatly improved mankind's capability of acquisition, processing and application of spatial information. The increase of spatial data sources and the development, applications and industrialization of spatial information technology are urging the need of spatial data sharing and exchanging. Based an a brief introduction an the China Spatial Information Network (CSI) and its database system, the CSI Spatial Science and Technology Information Database (SSTID) management system was designed and implemented in this paper.