Serial values for hematologic and biochemical analysis after myocardial infarction in rats

( Mi Jin Lee ),( Hyun Jin Tae ),( Ying Hua Li ),( Do Hyeon Yu ),( In Ae Han ),( Seok Won Lee ),( Dong Choon Ahn ),( In Shik Kim ),( Jin Ho Park ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction(MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hema-tologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and bioche-mical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery(LMDA) of the left coronary artery was ligated during the progression(day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group(n=24, thoracotomy without LMDA ligation) and the experimental(MI) group(n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell(RBCs), hemoglobin and hematocrit(HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, choles-terol, total creatine kinase(CK) and isoenzyme MB, and lactate dehydrogenase(LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Serial values for hematologic and biochemical analysis after myocardial infarction in rats

Lee, Mi-Jin,Tae, Hyun-Jin,Li, Ying-Hua,Yu, Do-Hyeon,Han, In-Ae,Lee, Seok-Won,Ahn, Dong-Choon,Kim, In-Shik,Park, Jin-Ho The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction (MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hematologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and biochemical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery (LMDA) of the left coronary artery was ligated during the progression (day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group (n=24, thoracotomy without LMDA ligation) and the experimental (MI) group (n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell (RBCs), hemoglobin and hematocrit (HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, cholesterol, total creatine kinase (CK) and isoenzyme MB, and lactate dehydrogenase (LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

심근경색의 위험인자로서 Thrombomodulin유전자변이의 의의

박현영,김영미,권혁문,지선하,조승연,정남식,심원흠,장양수 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.6

미세각막절개 백내장 수술 후 낭내 삽입한 일체형 비구면 인공수정체의 임상 결과

김윤전,전미현,고동아,김재용,김명준,차흥원,Yoon Jeon Kim,Mi Hyun Cheon,Dong Ah Ko,Jae Yong Kim,Myoung Joon Kim,Hung Won Tchah 대한안과학회 2013 대한안과학회지 Vol.54 No.4

Purpose: To evaluate the stability and optical performance of the newly developed single-piece aspheric intraocular lens (IOL) by comparing the clinical outcome of the aspheric IOL with the new optic profile design (HOYA iSert, HOYA iMics) and the aspheric IOL (Akreos MI60), which has been proven effective and safe. Methods: iSert, iMics, and MI60 were inserted into 55 eyes, 60 eyes, and 50 eyes, respectively, after microincision phacoemulsification cataract surgery. Best corrected visual acuity (BCVA), refraction in spherical equivalent, anterior chamber depth (ACD), total higher order aberration (HOA), contrast sensitivity, and surgically induced astigmatism (SIA) were measured and each IOL was evaluated on the functional stability, anterior-posterior stability, centration in the capsular bag, and quality of vision. Results: No statistical differences in preoperative and postoperative BCVA among the 3 IOL groups were observed, however, MI60 showed significant myopic shift postoperatively. Anterior-posterior stability assessed with postoperative change in refractive error and ACD was slightly lower in the MI60 group. In terms of vision quality, while total aberration, total HOA, coma aberration, and contrast sensitivity for the 3 IOLs were not different significantly, spherical aberration of the MI60 group was higher than the other groups at 6 months postoperative. SIA was significantly increased in eyes implanted with iSert than in eyes with iMics or MI60 at 1 month postoperatively, however, the differences were no longer evident after 3 months postoperatively. Conclusions: The new aspheric IOLs, iSert and iMics, showed good stability and visual outcome equal to MI60 at the 6-month postoperative follow-up.

갈등사회와 사회과학 방법론 : Gilbert Abcarian Monte Palmer의 Society in Conflict, An Introducation to Social Science를 중심으로

원미경 이화여자대학교 법정대학 비서학과 1985 비서학연구 Vol.- No.5

한지 쌈지 극미량 고체상 추출법을 이용한 레몬과 오렌지 향유 성분 분석

원미미,이동선 서울여자대학교 2010 자연과학연구논문집 Vol.22 No.-

In this study, headspace mulberry paper bag micro solid phase extraction (HS-MPB-μ-SPE) combined to gas chromatography/mass spectrometry has been applied for the analysis of volatile aromas of liquid essential oils from lemon and orange. The technique uses an adsorbent of Tenax TA contained in a mulberry paper bag, minimal amount of organic solvent and very small amount (10 μL) of sample size. Linearity for the calibration curve was excellent, the LOD value was 10.3 ng/mL. Precision and recovery were generally good. In addition, this method allowed combining extraction, enrichment, and clean-up in a single step. Analysis of aroma components in lemon and orange essential oils were performed successfully using this method.

십대/여성 정책의 도전과 과제 : 적극적 조치, 실질적 평등의 관점을 중심으로

원미혜 이화여자대학교 한국여성연구원 2007 여성학논집 Vol.24 No.2

본 논문은 이제까지 진행되어 온 십대여성 관련 정책이 ‘청소년 성보호’라는 특정한 이슈로만 다루어져 왔을 뿐, 중요한 정책 대상으로 접근되지 않았다는 문제의식에서 출발하여, 십대/여성 정책의 필요성, 목표와 내용 그리고 방향성을 타진해 보고자 했다. 먼저, 적극적 조치의 관점에서 볼 때, 십대/여성은 성별직종분리, 여성차별적인 사회통념을 변화시키기 위한 효과적인 전략 범주라는 점에서 성 주류화를 위한 ‘특별한 고려’의 대상으로 상정될 필요가 있다 한편, 십대/여성 정책은 시민권을 갖지 못한 ‘십대’라는 시기의 차별을 문제 삼는다는 점에서 열악한 인권을 우선적으로 고려하는 복지적 접근을 요구한다. 그러나 그것이 잔여주의적이고, 성인중심적인 ‘보호’ 정책으로 한정될 때, 새로운 도전은 불가능하다. 성매매, 가출, 임신, 약물 등 문제가 되는 개별 사안에 치우치기보다는 일관적이며 통합적인 관점이 필요하다. 기존의 ‘소극적ㆍ방어적 접근’에서 ‘적극적ㆍ긍정적 접근’으로의 전환이 요구되는 것이다. 따라서 십대/여성 정책은 ‘성(평등) 민감성’, ‘다른 사회적 정체성에 대한 고려’, ‘십대/여성의 참여와 공유’라는 통합된 관점이 정책 개발단계에서 시행, 평가에 이르기까지 일관적으로 녹아있어야 할 것이다. 한편 십대들의 ‘목소리’를 억누르는 서열과 위계질서를 성찰하고 다양한 차이들을 고려할 때, 십대/여성 정책은 평등사회를 위한 새로운 도전이 될 것이다. This thesis is based on the problem that issues revolving around teenage women have been dealt with only within the limited context of "protecting adolescent sexuality." This study is thus designed not only to argue the necessity of specific policies targeting teenage women but to discuss policy missions, direction, contents, and challenges. Taking the affirmative action perspective, teenage women as a target of the policy need to be redefined as the object of "differential consideration," in light of changing the generally accepted notion rooted in sexism and occupational gender segregation. Meanwhile, we also need to recognize the fact that the teenage women policy should be engaged with a social welfare perspective on human rights, noting the fact that teenage women have been discriminated against based on their age, being denied their citizenship as members of a society. However, it is crucial to take into account that this policy should be challenged due to the form it has maintatined: protective, adult-centric, and residual. The experiences and issues that teenage women have are intrinsically linked instead of being fragmented. Thus there needs to be an integrated viewpoint which adopts the notion that issues such as adolescent prostitution, teenage runaway, pregnancy, and substance abuse are interlocking and interrelated. To achieve substantial equality, we should allow a "positive/progressive approach" to emerge instead of a "negative/regressive approach." Accordingly, teenage women policies are required to fully incorporate relevant perspectives including gender (equality) awareness, differential consideration of alternative social identities, and participation and sharing of teenage women, throughout the process of making and implementing the policy. In addition, recognizing the limits of the policy which has mainly focused on the wellbeing of adults, not teenage women, the policy on teenage women should alternatively stand for the "ethics of relationships" between adults and teenagers. By reflecting on various differences among teenage women as well as reconsidering the hierarchy which makes the voice of teenage women unheard, only then can the policy take a new level of challenging the present condition of teenage women and their human rights as the underprivileged.

標識放鳥에 依한 韓國産 철새 集團의 季節的 分布와 그의 生態(Ⅰ)

元炳旿,禹漢貞,咸奎晃,田美子,尹茂夫 慶熙大學校 1967 論文集 Vol.5 No.-

實驗材料로 토기, 마우스 및 가물치를 使用하여서 致死至近量의 物理的 環境要因 (溫度, 重力, 電擊, 電離放射線 및 非電離放射線), 化學的 環境要因(無機이온) 및 生物學的 環境要因(媒質의 渗透壓)等에 依한 處理가 血液蛋白質의 電氣泳動像을 비롯하여 赤血球, 白血球, 헤마토크리트比, 血色素量, 赤血球脆弱性, 肝臟器官의 組織像 그리고 肝臟 및 腎臟器官重量等에 미치는 效果를 硏究調査하였다. 前記 致死至近量의 環境要因의 處理로 一般的으로 蛋白質代謝, 血液像, 肝臟器官의 組織像 및 肝臟器官重量等에 顯著한 變化를 招來함을 알 수 있었다. 總血淸蛋白質量의 顯著한 變化 및 알부민―굴로부린比의 變化等은 血液內의 渗透效果의 變化를 招來함을 示唆하여 준다. 血淸電氣泳動像의 變化는 前記 環境要因에 依한 處理로 因하여 蛋白質代謝의 異常 卽 肝臟機能의 異常을 招來함을 알 수 있다. 以上의 結果로 미루어 보아 致死至近量이 內的 및 外的의 物理的, 化學的및 生物學的 環境要因에 依한 處理로 因하여 血液蛋白質, 血液像 및 肝臟器官 等의 異常은 生體에 큰 損傷을 가져오며 아울러 生體의 生理的平衡에 有意性的인 變化를 招來한다고 思料된다. Seasonal distribution and ecology of migrant bird populations were studied by mist-netting and banding primarily in the ares of Kyunggido, Korea during 1963-1966. 1. From 6 June to 25 June 1963, 99 birds of 3 species and 3 July 1964 to 31 December 1966, a total of 123, 242 birds of 124 species were banded in Korea. 196 (144 Returns) recoveries of 22 species have been reported in Korea (outside of banding sites) and 7 recoveries of 5 species from abroad. 2. At Taenung, N.E. Seoul, Korea, 11623 birds of the Pied Wagtail and 9,013 birds of the House Swallow were banded in pear orchards. a. Both Wagtail and Swallow are summer resident Wagtail arrives Korea in the beginning of March and Swallows in early April and these gregarious species gather at night into large flocks to roost while juveniles maintain their post-breeding roosts in pear orchard from June to October until the time of Autumn migration. b. Both Wagtaill and Swallow maintain their roosts in the same site but they exhibit different roosting behavior. c. The time at which the Wagtails and Swallows arrive and leave the roost in relation to sunset varies with length of day, weather, however, light intensity mat remain the same. d. The Wagtail gathers around the feeding ground more than 20 kilometers from the roosting site. e. Some Wagtails and Swallows banded the previous year returned and roosted in the orchard. The fact that they repeatedly roost in the same areas suggest that after breeding some birds remain in the Orchard and some of them migrate southward. 3. During July 1964-October 1966, 78, 170 birds of 12 Emberiza species were banded primarily in Kyunggi-do. Emberiza rutila, Emberiza spodocephala, Emberiza tristrami and Emberiza aureola ornata are the dominant fall and spring migrants. In fall they prefer soy-bean, corn and especially millet fields while in spring they are mostly seen on wheat and barley fields. Emberiza rustica is the most abundant species during late fall and winter and they prefer open fields with bushes. Emberiza rutila migrates through Korea in May and from the beginning of August until the and of October. The sex ratio is 100 females to 155 males (11674 ♀, 17761 ♂). More males were caught than females each month except in September when more females were caught. Emberiza spodocephala migrates through Korea from the middle of April through the middle of May and from the meddle of September through October. Emberiza tristrami migrates southward through Korea the first half of May and during October. The sex ratio is 100 female to 140 males(392 ♀, 551 ♂). Emberiza aureola oruata megrates through Korea during May nd from early August until the end of October. Emberiza rustica migrates southward but some of them winter in Korea. The wintering period is from the early October until the end of April. The sex ratio is 100 females to 191 mules (13,450 ♀, 25,687 ♂). Emberiza yessoensis continentals migrates to Korea around the middle of October. Some of them wander in Korea while some of them migrate southward. The wandering period is from the middle of October to the meddle of February. Emberiza cioidos is a permanent resident and breeds in great number in Korea. However, they migrate southward in large flocks during winter and northward during spring. The sex ratio is 100 females to 159 males (497 ♀, 792 ♂). Emberiza e. elegans is a resident and breeds in fairly small number in Korea. but they migrate southward in large flocks around the end of October and northward around April. The sex ratio is 100 females to 280 males(462 ♀, 962 ♂). Emberiza f. fucata is a summer resident, arrives to Korea the middle of April and most of them migrate southward in September. Emberiza chrysophtys migrates southward through Korea in May and from September to October. Emberiza pusilla is a not uncommon transient during spring and autumn and some of it wanders during winter in Korea. Emberiza leucocephala leucocephala Known as a straggler based on the sex specimens but a rare winter visitor. It migrates southward through Korea and some of it wanders around the end of January to the middle of March. 4. Noteworthy records of 21 species observed by the authors and some new species in Korea are given, each with notes on banding and collection records-See text for each species. Observations were made on the feeding habits of nestlings of ten species, Lanius tigrinus, Butorides striatus amurensis, Fhnberiza f. fucata, Motazilla alba leucopsis, Oriolus chinensis diffusns. Accipiter nisus nisosimilis, Mierascelis amaurotis henssent. Garrulus glandarins brandtii, Accipiter solecnsis and Cyanopica eyamts Koreensis. The investigation was made in Kwangnung experimental forest, Kyunggido and the nearby open field by using collar method. Accipiter nisus nisosimiles, Microscelis amaurotis hensoni, Garrulus glandarius brandtii and Cyanopiea cyanus Koreenses are permanent residents and the other six species are common summer residents. The following is the food that these nestlings consumed. 1. Lanius tigrinus The food they consumed was animal matter Composes of; insect larvae-41.5%, insect adults-49.4%, spiders-7.69%, frogs-1.53%. The cicada, Gampsocleis ussuricnsis made up 33.8% of the insect larvae. The cricket, Platyplenra kacmpferi made up 35.4% of the adult insects. Only one species of spiders, Clubiona jucunda was seen, 7.69% of the food. 2. Butorides striatus amurensis The food was animal matters composed of; small freshwater fishes-18.57%, Ranidae-45.71%, others-5.71%. Twenty percent of the small freshwater fishes was Zacco platypus while Hemibarbus logirostris made up 14.28%. Among Amphibians, Rana n. nigromaculata included 22.8%. 3. Emberiza f. fucata The food was amimal matter; insect larvae-63.3%, adult insects-25.64%, others-12.78%, Since 48.1% of the food items was larvae of Pieris rapae, it is most useful for agriculture. 4. Motacilla alba larcopsis The food during their nestling period was animal matter compsed of; insect larvae-30.5%, adult insects-55.4%, spiders-13.9%. The commonest insect larvae were Odonata indet.-22.2%. The commonest adult insects were Syrphidae indet.-16.6%. Of the spiders Lyecsa sp. made up 12.1% and Lycosa astrigera-2.8%. 5. Oriolus chinensis diffusus The food was animal matter composed of; insect larvae-62.36%, adult insects-20.17%, others017.42%. It should be noted that from the initial time of its feeding until leaving it consumed Dendrolimus spectabilis, a noxious forest insect which made up 45.08% of the diet. Thus it is very useful birds eliminating noxious forest insects. 6. Accipiter nisus nisosimilis The food was animal matter composed of; small passerine birds-87.5%, Ranidac-12.05%. It consumed such forest birds as Paridae. 7. Microscelis amauretis hensani The food was primarily insect adults-79.65%, Mollusca-12.15%, vegetable matter-4.05%, insect larvae-2.70%. Araneina-1.35%. Homoptera was 43.35% of adult insects. 8. Garrulus glaudarins brandtii The food during its nestling pcriod was solely animal matter; insect larvae-38.80%, adult insects-28.90%, Araneina-24.87%, adult Amphibia-15.49%. Right afrer hatehing and until leaving its nest, Dendrolimus spectabilis-35.08%, Arancina-24.87%, Clubiona jueunda-6.43% were fed. Therefor 73.48% of the total food was noxious forestry insects. 9. Accipiter soloensis The food during the whole feeding period was primarily Rana n. nigroma-culata 89.09%, but small quantity of Platypleura Kaempferi 8.26%, was also fed. 10. Cyanopica cyanus koreensis The food was composed of; insect larvae-6.11%, adult insects-60.55%, Hyla arborea japonica 12.22%, vegetable matter-1.11%. Gampsocleis ussuriensis-23.39%, Platypleura knempferi-15.55%, and Hyla arborea japoniea-12.22% were the prefered food supplied during the whole feeding period.

SPME와 GC/MS를 이용한 녹나무 향기성분 특성분석

원미미,차은주,윤옥경,김남선,김건,이동선 서울여자대학교 자연과학연구소 2007 자연과학연구논문집 Vol.19 No.-

Recently, land managers have come to realize that, outside Asia, camphor trees grow like street-tree or weeds, infesting forests and displacing native trees. This is because camphor trees are prolific seed producers that do not have serious predators or diseases outside their native range. The camphor tree (Cinnamomum camphora), belongs to lauraceae, is the aromatic evergreen tree from which camphor was derived. Headspace solid-phase microextraction (HS-SPME) and GC-MS were applied for the analysis of volatile fragrances emanated from camphor tree. Divinylbenzene / Carboxen / PDMS fiber was used for HS-SPME adsorption materials. (+)-Camphor (31.00%) and cineol (21.89%) were found as the major volatile aroma components from Chinese camphor tree, whereas (+)-camphor (62.06%) and β-caryophyllene (10.01%) from Japanese camphor tree. Camphor tree is a promising garden tree for well-being.