Insights Into the BR2/PGP1-mediated Patterns for Shoot and Root Growth in Maize Early Seedling Development by Comparative Transcriptome Sequencing

Xiangge Zhang,Lanjie Zheng,Haojun Zhang,Xinrong Huang,Yinghong Liu,Junjie Zhang,Yu-Feng Hu,Hanmei Liu,Guowu Yu,Yangping Li,Yubi Huang 한국식물학회 2019 Journal of Plant Biology Vol.62 No.3

Plant shoot and root growth is regulated by multiplefactors, including the hormone auxin. A maize brachytic2(br2) mutant displays a shortened shoot and root with highauxin concentration in their apexes, where BR2 encodes anauxin transporter P-glycoprotein1 (PGP1). We employedcomparative transcriptome sequencing to explore the BR2/PGP1-mediated patterns for shoot and root growth amongbr2, wild type (WT), and hybrid F1 (br2 × WT). The resultsshow 352 genes in the br2 shoot and 234 genes in the br2root are differentially expressed compared to WT and F1. Zm00001d045203 and Zm00001d031562 were identified astwo auxin-responsive genes in both the shoot and root. TheZm00001d045203 gene is down-regulated in br2 and encodesan Aux/IAA inhibitor involved in the SCFTIR1/AFB-mediatedauxin signaling pathway. The up-regulated Zm00001d031562gene encodes a small GTPase belonging to the Rab/Yptfamily, which takes part in auxin-mediated responses inArabidopsis. Cytology analysis reveals that reduced cellelongation affects the shoot and root growth of br2. TheZm00001d043477 gene is down-regulated and encodes amember of the cellulose synthase protein family, possiblyreducing cell elongation in the br2 shoot and root. Theseresults reveal the specific regulation of auxin actions and cellresponses by BR2/PGP1.

Mechanical and Thermodynamic Properties of Unidirectional Flax Fiber Reinforced CNT Modified Epoxy Composites

Hongguang Wang,Lanjie Yang,Huajie Guo,Yagebai Zhao,Jianing Zhao 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6

In this paper, the mechanical and thermodynamic properties of diglycidyl ether of bisphenol-F (DGEBF) epoxyresin and flax fiber FRP composites, which was modified with multi-walled carbon nanotubes (MWCNTs) were studied. Themechanical property tests show significant improvements in the tensile strength (48.7 %), tensile modulus (25.2 %) at1.0 wt.% of MWCNTs. DMTA test reveals that mixture of 1.0 wt.% MWCNTs into the epoxy resin increases 10.68 K of Tg,which is in accordance to the simulated results from molecular dynamics. These improvements in mechanical andthermodynamic properties are attributed to the balanced effect of stress concentration and radiation. The mechanical andthermodynamic properties of flax fiber FRP composites are improved with the addition of MWCNTs. This study is expectedto provide an opportunity to develop high performance of natural fiber FRP composites.

C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells

Lei Yang,Lanjie Lei,Qihan Zhao,Ying Gong,Gaopeng Guan,Shaoxin Huang 대한남성과학회 2019 The World Journal of Men's Health Vol.37 No.2

Purpose: This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secre-tion in mouse Leydig cells.Materials and Methods: Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its receptor NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Leydig cells isolated from mouse testis were cultured and treated with shNPR2 lentiviruses or CNP. And then the cyclic guanosine mono-phosphate production, testosterone secretion, cell proliferation, cell cycle and cell apoptosis in mouse Leydig cells were ana-lyzed by ELISA, RT-qPCR, Cell Counting Kit-8, and flow cytometry. Moreover, the expression of NPR2, cell cycle, apoptosis proliferation and cell cycle related gene were detected by RT-qPCR and Western blot. Results: Knockdown of NPR2 by RNAi resulted in S phase cell cycle arrest, cell apoptosis, and decreased testosterone secre-tion in mouse Leydig cells.Conclusions: Our study provides more evidences to better understand the function of CNP/NPR2 pathway in male reproduc-tion, which may help us to treat male infertility.

Mechanical and Interfacial Properties of Flax Fiber-reinforced Plastic Composites Based on a Chemical Modification Method

Hongguang Wang,HAO WU,Lanjie Yang,Guanglong Yu 한국섬유공학회 2020 Fibers and polymers Vol.21 No.7

Due to growing interest in environmental protection, eco-friendly fibers with high specific strength and low costare widely used to reinforce matrix materials. Flax fiber is a natural renewable plant fiber that originates from the phloem offlax. Flax fibers not only have the characteristics of other natural fibers but also have the outstanding mechanical properties ofnatural fibers. However, due to the large amount of hydrophilic hydroxyl groups on the surface, the hydrophilic absorption offlax fibers is large, and the wet-heat durability of the composite is poor, which limits the use of flax fiber as a reinforcementmaterial in civil engineering applications. In this study, based on the research results of flax fiber, a flax fiber sheet wasgrafted with multiwalled carbon nanotubes, a silane coupling agent and nano-TiO2 particles. The effect of different treatmentmethods and process parameters on the mechanical and interfacial properties of flax fiber-reinforced plastic (FFRP)composites materials was studied. The results show that the FFRP composites grafted with multiwalled carbon nanotubeshave higher tensile strength and interlaminar shear strength than those grafted with the same content of nano-TiO2 particles. Flax fiber composites with improved interfacial properties can be obtained by grafting flax fiber sheets, but the content ofparticles on the flax fiber surface should not exceed 2.0 wt.% of nano-TiO2 and multiwalled carbon nanotubes. Under thistreatment, the FFRP composites have improved properties, such as tensile strength, interlaminar shear strength and glasstransition temperature.

Study on Microbial Community Succession and Protein Hydrolysis of Donkey Meat during Refrigerated Storage Based on Illumina NOVA Sequencing Technology

Zixiang Wei,Ruidong Chu,Lanjie Li,Jingjing Zhang,Huachen Zhang,Xiaohong Pan,Yifan Dong,Guiqin Liu 한국축산식품학회 2021 한국축산식품학회지 Vol.41 No.4

In this study, the microbial community succession and the protein hydrolysis of donkey meat during refrigerated (4℃) storage were investigated. 16S rDNA sequencing method was used to analyze the bacteria community structure and succession in the level of genome. Meanwhile, the volatile base nitrogen (TVB-N) was measured to evaluate the degradation level of protein. After sorting out the sequencing results, 1,274,604 clean data were obtained, which were clustered into 2,064 into operational taxonomic units (OTUs), annotated to 32 phyla and 527 genus. With the prolonging of storage time, the composition of microorganism changed greatly. At the same time, the diversity and richness of microorganism decreased and then increased. During the whole storage period, Proteobacteria was the dominant phyla, and the Photobacterium, Pseudompnas, and Acinetobacter were the dominant genus. According to correlation analysis, it was found that the abundance of these dominant bacteria was significantly positively correlated with the variation of TVB-N. And Pseudomonas might play an important role in the production of TVB-N during refrigerated storage of donkey meat. The predicted metabolic pathways, based on PICRUSt analysis, indicated that amino metabolism in refrigerated donkey meat was the main metabolic pathways. This study provides insight into the process involved in refrigerated donkey meat spoilage, which provides a foundation for the development of antibacterial preservative for donkey meat.

Safety Ontology Modeling and Verification on MIS of Ship-Building and Repairing Enterprise

( Yumei Wu ),( Zhen Li ),( Lanjie Zhao ),( Zhengwei Yu ),( Hong Miao ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.4

Shipbuilding and repairing enterprise has the characteristics of many hazards and accidents. Therefore, the safety management ability of shipbuilding and repairing MIS (management information system) must be effectively guaranteed. The verification on safety management is the necessary measure to ensure and improve the safety management ability of MIS. Safety verification can not only increase the safety of MIS, but also make early warning of potential risks in management to avoid the accidents. Based on the authoritative standards in the field of safety in shipbuilding and repairing enterprise, this paper applied modeling and verification method based on ontology to safety verification of MIS, extracted the concepts and associations from related safety standards to construct axiom set to support safety verification on MIS of shipbuilding and repairing enterprise. Then, this paper developed the corresponding safety ontology modeling and verification tool-SOMVT. By the application and comparison of two examples, this paper effectively verified the safety of MIS to prove the modeling method and the SOMVT can improve the safety of MIS in a much more effective and stable way to traditional manual analysis.

Different divergence events for three pairs of PEBPs in Gossypium as implied by evolutionary analysis

Youjun Lu,Wei Chen,Lanjie Zhao,Jinbo Yao,Yan Li,Weijun Yang,Ziyang Liu,Yongshan Zhang,Jie Sun 한국유전학회 2019 Genes & Genomics Vol.41 No.4

Introduction The phosphatidylethanolamine-binding protein (PEBP) gene family plays a crucial role in seed germination, reproductive transformation, and other important developmental processes in plants, but its distribution in Gossypium genomes or species, evolutionary properties, and the fates of multiple duplicated genes remain unclear. Objectives The primary objectives of this study were to elucidate the distribution and characteristics of PEBP genes in Gossypium, as well as the evolutionary pattern of duplication and deletion, and functional differentiation of PEBPs in plants. Methods Using the PEBP protein sequences in Arabidopsis thaliana as queries, blast alignment was carried out for the identification of PEBP genes in four sequenced cotton species. Using the primers designed according to the PEBP genome sequences, PEBP genes were cloned from 15 representative genomes of Gossypium genus, and the gene structure, CDS sequence, protein sequence and properties were predicted and phylogenetic analysis was performed. Taking PEBP proteins of grape as reference, grouping of orthologous gene, analysis of phylogeny and divergence of PEBPs in nine species were conducted to reconstruct the evolutionary pattern of PEBP genes in plants. Results We identified and cloned 160 PEBPs from 15 cotton species, and the phylogenetic analysis showed that the genes could be classified into the following three subfamilies: MFT-like, FT-like and TFL1-like. There were eight single orthologous group (OG) members in each diploid and 16 double OG members in each tetraploid. An analysis of the expression and selective pressure indicated that expression divergence and strong purification selection within the same OG presented in the PEBP gene family. Conclusion An evolutionary pattern of duplication and deletion of the PEBP family in the evolutionary history of Gossypium was suggested, and three pairs of genes resulted from different whole-genome duplication events.

Emerging 3D bioprinting applications in plastic surgery

Yang Pu,Ju Yikun,Hu Yue,Xie Xiaoyan,Fang Bairong,Lei Lanjie 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Plastic surgery is a discipline that uses surgical methods or tissue transplantation to repair, reconstruct and beautify the defects and deformities of human tissues and organs. Three-dimensional (3D) bioprinting has gained widespread attention because it enables fine customization of the implants in the patient's surgical area preoperatively while avoiding some of the adverse reactions and complications of traditional surgical approaches. In this paper, we review the recent research advances in the application of 3D bioprinting in plastic surgery. We first introduce the printing process and basic principles of 3D bioprinting technology, revealing the advantages and disadvantages of different bioprinting technologies. Then, we describe the currently available bioprinting materials, and dissect the rationale for special dynamic 3D bioprinting (4D bioprinting) that is achieved by varying the combination strategy of bioprinting materials. Later, we focus on the viable clinical applications and effects of 3D bioprinting in plastic surgery. Finally, we summarize and discuss the challenges and prospects for the application of 3D bioprinting in plastic surgery. We believe that this review can contribute to further development of 3D bioprinting in plastic surgery and provide lessons for related research. Graphical Abstract

Emerging Biomaterials for Tumor Immunotherapy

Minna Xiao,Qinglai Tang,Shiying Zeng,Qian Yang,Xinming Yang,Xinying Tong,Gangcai Zhu,Lanjie Lei,Shisheng Li 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background The immune system interacts with cancer cells in various intricate ways that can protect the individual from overproliferation of cancer cells; however, these interactions can also lead to malignancy. There has been a dramatic increase in the application of cancer immunotherapy in the last decade. However, low immunogenicity, poor specificity, weak presentation efficiency, and off-target side effects still limit its widespread application. Fortunately, advanced biomaterials effectively contribute immunotherapy and play an important role in cancer treatment, making it a research hotspot in the biomedical field. Main body This review discusses immunotherapies and the development of related biomaterials for application in the field. The review first summarizes the various types of tumor immunotherapy applicable in clinical practice as well as their underlying mechanisms. Further, it focuses on the types of biomaterials applied in immunotherapy and related research on metal nanomaterials, silicon nanoparticles, carbon nanotubes, polymer nanoparticles, and cell membrane nanocarriers. Moreover, we introduce the preparation and processing technologies of these biomaterials (liposomes, microspheres, microneedles, and hydrogels) and summarize their mechanisms when applied to tumor immunotherapy. Finally, we discuss future advancements and shortcomings related to the application of biomaterials in tumor immunotherapy. Conclusion Research on biomaterial-based tumor immunotherapy is booming; however, several challenges remain to be overcome to transition from experimental research to clinical application. Biomaterials have been optimized continuously and nanotechnology has achieved continuous progression, ensuring the development of more efficient biomaterials, thereby providing a platform and opportunity for breakthroughs in tumor immunotherapy.

The Positive Association between Subclinical Hypothyroidism and Newly-Diagnosed Hypertension Is More Explicit in Female Individuals Younger than 65

Xichang Wang,Haoyu Wang,Li Yan,Lihui Yang,Yuanming Xue,Jing Yang,Yongli Yao,Xulei Tang,Nanwei Tong,Guixia Wang,Jinan Zhang,Youmin Wang,Jianming Ba,Bing Chen,Jianling Du,Lanjie He,Xiaoyang Lai,Yanbo Li 대한내분비학회 2021 Endocrinology and metabolism Vol.36 No.4

Background: Subclinical hypothyroidism (SCH) is the most common thyroid dysfunction, and its relationship with blood pressure(BP) has been controversial. The aim of the study was to analyze the association between SCH and newly-diagnosed hypertension. Methods: Based on data from the Thyroid disease, Iodine nutrition and Diabetes Epidemiology (TIDE) study, 49,433 euthyroid individuals and 7,719 SCH patients aged ≥18 years were enrolled. Patients with a history of hypertension or thyroid disease were excluded. SCH was determined by manufacturer reference range. Overall hypertension and stage 1 and 2 hypertension were diagnosedaccording to the guidelines issued by the American College of Cardiology/American Heart Association in 2017. Results: The prevalence of overall hypertension (48.7%), including stage 1 (28.9%) and 2 (19.8%) hypertension, increased significantly in SCH patients compared with euthyroid subjects. With elevated serum thyroid stimulating hormone (TSH) level, the hypertension prevalence also increased significantly from the euthyroid to different SCH subgroups, which was more profound in femalesor subjects aged <65 years. The age- and sex-specific regression analysis further demonstrated the same trends in the general population and in the 1:1 propensity matched population. Similarly, several BP components (i.e., systolic, diastolic, and mean arterial BP)were positively associated with TSH elevation, and regression analysis also confirmed that all BP components were closely relatedwith SCH in female subjects aged <65 years. Conclusion: The prevalence of hypertension increases for patients with SCH. SCH tends to be associated with hypertension and BPcomponents in females younger than 65 years.