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Hongguang Wang,Lanjie Yang,Huajie Guo,Yagebai Zhao,Jianing Zhao 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6
In this paper, the mechanical and thermodynamic properties of diglycidyl ether of bisphenol-F (DGEBF) epoxyresin and flax fiber FRP composites, which was modified with multi-walled carbon nanotubes (MWCNTs) were studied. Themechanical property tests show significant improvements in the tensile strength (48.7 %), tensile modulus (25.2 %) at1.0 wt.% of MWCNTs. DMTA test reveals that mixture of 1.0 wt.% MWCNTs into the epoxy resin increases 10.68 K of Tg,which is in accordance to the simulated results from molecular dynamics. These improvements in mechanical andthermodynamic properties are attributed to the balanced effect of stress concentration and radiation. The mechanical andthermodynamic properties of flax fiber FRP composites are improved with the addition of MWCNTs. This study is expectedto provide an opportunity to develop high performance of natural fiber FRP composites.
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Lei Yang,Lanjie Lei,Qihan Zhao,Ying Gong,Gaopeng Guan,Shaoxin Huang 대한남성과학회 2019 The World Journal of Men's Health Vol.37 No.2
Purpose: This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secre-tion in mouse Leydig cells.Materials and Methods: Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its receptor NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Leydig cells isolated from mouse testis were cultured and treated with shNPR2 lentiviruses or CNP. And then the cyclic guanosine mono-phosphate production, testosterone secretion, cell proliferation, cell cycle and cell apoptosis in mouse Leydig cells were ana-lyzed by ELISA, RT-qPCR, Cell Counting Kit-8, and flow cytometry. Moreover, the expression of NPR2, cell cycle, apoptosis proliferation and cell cycle related gene were detected by RT-qPCR and Western blot. Results: Knockdown of NPR2 by RNAi resulted in S phase cell cycle arrest, cell apoptosis, and decreased testosterone secre-tion in mouse Leydig cells.Conclusions: Our study provides more evidences to better understand the function of CNP/NPR2 pathway in male reproduc-tion, which may help us to treat male infertility.
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Youjun Lu,Wei Chen,Lanjie Zhao,Jinbo Yao,Yan Li,Weijun Yang,Ziyang Liu,Yongshan Zhang,Jie Sun 한국유전학회 2019 Genes & Genomics Vol.41 No.4
Introduction The phosphatidylethanolamine-binding protein (PEBP) gene family plays a crucial role in seed germination, reproductive transformation, and other important developmental processes in plants, but its distribution in Gossypium genomes or species, evolutionary properties, and the fates of multiple duplicated genes remain unclear. Objectives The primary objectives of this study were to elucidate the distribution and characteristics of PEBP genes in Gossypium, as well as the evolutionary pattern of duplication and deletion, and functional differentiation of PEBPs in plants. Methods Using the PEBP protein sequences in Arabidopsis thaliana as queries, blast alignment was carried out for the identification of PEBP genes in four sequenced cotton species. Using the primers designed according to the PEBP genome sequences, PEBP genes were cloned from 15 representative genomes of Gossypium genus, and the gene structure, CDS sequence, protein sequence and properties were predicted and phylogenetic analysis was performed. Taking PEBP proteins of grape as reference, grouping of orthologous gene, analysis of phylogeny and divergence of PEBPs in nine species were conducted to reconstruct the evolutionary pattern of PEBP genes in plants. Results We identified and cloned 160 PEBPs from 15 cotton species, and the phylogenetic analysis showed that the genes could be classified into the following three subfamilies: MFT-like, FT-like and TFL1-like. There were eight single orthologous group (OG) members in each diploid and 16 double OG members in each tetraploid. An analysis of the expression and selective pressure indicated that expression divergence and strong purification selection within the same OG presented in the PEBP gene family. Conclusion An evolutionary pattern of duplication and deletion of the PEBP family in the evolutionary history of Gossypium was suggested, and three pairs of genes resulted from different whole-genome duplication events.
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Safety Ontology Modeling and Verification on MIS of Ship-Building and Repairing Enterprise
( Yumei Wu ),( Zhen Li ),( Lanjie Zhao ),( Zhengwei Yu ),( Hong Miao ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.4
Shipbuilding and repairing enterprise has the characteristics of many hazards and accidents. Therefore, the safety management ability of shipbuilding and repairing MIS (management information system) must be effectively guaranteed. The verification on safety management is the necessary measure to ensure and improve the safety management ability of MIS. Safety verification can not only increase the safety of MIS, but also make early warning of potential risks in management to avoid the accidents. Based on the authoritative standards in the field of safety in shipbuilding and repairing enterprise, this paper applied modeling and verification method based on ontology to safety verification of MIS, extracted the concepts and associations from related safety standards to construct axiom set to support safety verification on MIS of shipbuilding and repairing enterprise. Then, this paper developed the corresponding safety ontology modeling and verification tool-SOMVT. By the application and comparison of two examples, this paper effectively verified the safety of MIS to prove the modeling method and the SOMVT can improve the safety of MIS in a much more effective and stable way to traditional manual analysis.
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