Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

豚舍上部空間을 利用한 家兎飼育이 豚의 增體에 미치는 影響 : 1976-1979 第Ⅰ報-Ⅳ報 綜說 豚舍上部空間을 利用한 家兎飼育이 養豚 및 養兎兼業에 미치는 效果 1976-1979, Ⅰ-Ⅳ Abstract The effects of rabbit raising on the upper space of pig barn on the pig and rabbit production

鄭鉉丞,姜桂洪 진주산업대학교 1979 論文集 Vol.17 No.-

These experiments were carried out to determine the effects of liberal feeding of rabbit feces, urine and residual forage on the growth feed efficiency and production economy of pigs. This report was based on the results from 5 experiments from 1976 to 1979. The result obtained were summarized as follows: 1. The liberal feeding of rabbit feces, urine and residual forage to the weaned pigs and growing-finishing pigs resulted in the slight improvement in daily gain and feed efficiency and the decrease in feed cost per ㎏ of body gain. 2. The rabbit feces and residual forage contaminated with the rabbit urine were found to be palatable to pigs through the experiments. 3. The health of pigs consuming the rabbit waste was found normal clinically. 4. The Urea-N concentration of blood plasma was considerably in creased in the pigs consuming the rabbit waste. 5. The dressing percentage and the yield of pork cuts were not significantly changed by the feeding of rabbit waste and residual forage. From the above results it was concluded that the rabbit waste and residual forage be fed liberally by pigs with establishing the rabbit hatches over the pig barn and yard and this housing and feeding systems be beneficial to both rabbit and pigs production economy.

중학교 학생의 학교학습과 학원학습 문화에 관한 연구

변영계,김현주 釜山大學校 敎育硏究所 2001 敎育硏究 Vol.11 No.1

The purpose of this study is to describe and analyze the culture of students' learning in middle school. It has been regarded as one of the most distinctive educational phenomena in Korean students' culture composing school culture. Based on the ethnographic data collected, the study administered answer the following questions : 1. What activities do students do for learning in living and lessons of middle school, and what attitude and recognition do they have about learning? 2. What activities do students do for learning in living and lessons of 'HAKWON (cram school)', and what attitude and recognition do they have about learning? The major research method of this study is ethnographic interview. In addition, the researcher used the description survey, the document research, and a record of learning diary. The results of this study are as follows. First, students have the most important meaning on preparation for an examination in school learning. They devide 'the subjects with sample questions' from 'the subjects without sample questions' when they prepare for an examination. Besides, According to terms of examination, students concentrate on learning differently. Second, students want 'teaching by explanation'. It is the teaching pattern that teachers mainly explain the contents of textbooks. They want to understand perfectly and systematically the contents of textbooks. They dislike "only making progress". Third, the major purpose of 'cram school' is to make students advance academic achivement of students and it is related to strategy for existence of 1 cram school'. These results show that school on the public education cannot entirely accomplish educational function. A given condition of education and assessment system will have to be improved in order that school can stand on the center of education.

동양달팽이의 눈에 대한 해부학적 및 미세구조적 연구

정계헌,이현 한국패류학회 1994 The Korean Journal of Malacology Vol.10 No.1

고정화 및 저장 온도에 따른 Photobacterium phosphoreum의 Bioluminescence 안정성의 변화

김현숙,정계훈,전억한 경희대학교 생명자원과학연구원 1998 硏究論文集 Vol.19 No.-

P phosphoreum의 고정화에 있어 중요한 것은 matrix의 선택이며, matrix로서 soudium alginate만을 사용하여 고정화 하는 것보다는 strontium chloride를 첨가하여 gel의 견고성을 높여주었을 때 세포의 bioluminescence 유지도가 증가하였다. 저장온도에 따른 세포의 bioluminescence 유지도와 활성도와 관련하여 -70℃, -20℃, 20℃에서 저장한 세포의 경우 저장 1일 후에 급격한 bioluminescence의 감소를 보였으며 낮은 세포 활성도를 보인 반면 4℃의 경우 bioluminescence의 유지도가 15일 이상 이어졌으며 높은 활성도를 나타내었다. 따라서 P phosphoreum의 bioluminescence 안정성에 있어 가장 좋은 결과를 나타낸 것은 2.5%(W/W) sodium alginated와 0.3M(W/V) strontium chloride로 고정화하여 4℃에 저장한 세포였다. The objective of this work was to unprove biolummescence stability of Photobacterium phosphoreum when stored at different temperature in view of developing contmuous on-line monitonng system for pollutants m environment. A long-term experiment was performed to determine how immobilization affects the mamtenance and stability of biolummescence from luminescent bacteria at appropnate temperature. The unmobhzed cells of P. phosphoreum were compared with free cells m terms of mamtenance of biolummescence at room temperature. It was found that the biolummescence of cells immobilized on strontium alginate showed higher biolummescence mtensity than both free and mixed cells with only algmate as a matrix. The effect of temperature on the biolummescence stability was investigated with free and immobilized cells stored at 20℃, 4℃, -20℃ and -70℃ for 20 days. Both free and immobilized cells stored at 4℃ emtted a stable biolummescence whde the biolummescence markedly decreased with those stored at 20℃, -20℃ and -70℃.

퍼지 데이터를 이용한 불량률(Ρ) 관리도의 설계

김계완,서현수,윤덕균 한국품질경영학회 2004 품질경영학회지 Vol.32 No.2

Using the p chart is not adequate in case that there are lots of data and it is difficult to divide into products conforming or nonconforming because of obscurity of binary classification. So we need to design a new control chart which represents obscure situation efficiently. This study deals with the method to performing arithmetic operation representing fuzzy data into fuzzy set by applying fuzzy set theory and designs a new control chart taking account of a concept of classification on the term set and membership function associated with term set.

AR기법을 이용한 SLAM 영상 개선

김현,송대건,황기환,연규헌,전계석 慶熙大學校 大學院 1998 高凰論集 Vol.22 No.-

In this paper, we have been studied the enhancement of SLAM image using average response methos. AR methos has been used to reduce the random noise in signal processing. In this the experiment, we have constructed the SLAM system of 10㎒ operating frequency and experienced for holed Al sample. In this experimental result, we have been found that SLAM using AR method was able to get the high-speed and enhanced image compared with SAM.

開發制限區域制度(Green Belt)의 問題點과 合理的인 利用方案에 관한 硏究

李啓鶴,金在國,金昌炫 호남대학교산업기술연구소 1994 산업기술연구논문집 Vol.1 No.-

1970年 이래 우리나라의 急速한 經濟成長은 都市化와 産業化를 수반하였고 結果的으로 都市로의 人口集中과 숱한 都市問題를 야기시켰으며, 이러한 問題를 合理的으로 대처하기 위하여 政府는 지난 20여년에 걸쳐 英國에서 처음으로 實施했던 Green Belt制度를 採擇했다. 우리나라의 Green Belt制度는 1971년 7월 30일을 기하여 서울을 비롯해서 全國 13個 主要都市에서 처음으로 實施하였다. 本 論文에서는 우리나라에서 처음 Green Belt設定으로 말미암아 거기에 수반된 숱한 問題點에 대하여 이를 各 側面에서 檢討·分析하고 나아가 管理面에서 發生한 여러 가지 問題를 解消하기 위한 合理的인 利用方案을 提示하였다. 따라서 「制限된 國土資源의 效率的인 利用」이라는 觀點에서 Green Belt의 合理的인 利用方案이 講究되어야 할 것이다. Rapid economic growth of Korea since 1970's has accompanied urbanization and industrialization, resulting over population, an urban problem. To cope with this problem effectively, government has being adopted Green Belt policy originated in Great Britain in the past 20years. Green Belt areas were for the first time established in 13 major cities including Seoul, on July 30 1971. This paper aimes to present rational plan, countermeasures and management problems, resulting from the establishment of Green Belt, of which several analysis were made in all it's aspects. Therefore, we should consider a counter plan and rational utilization method of Green Belt in a point of view of national land use.

필리핀 여행 후 발생한 쯔쯔가무시병 1예

김계형,김낙현,김문석,김충종,전재현,박완범,장원종,박상원,김익상,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Infectious diseases imported from other countries have increased as more and more Koreans are going abroad for various purposes. Tsutsugamushi disease from other endemic area such as Southeast Asia is important, because it can occur in any season and eschar may be absent. We report a case of imported tsutsugamushi disease acquired in the Philippines. A patient presented with fever, headache, and maculopapular skin rash. However, eschar was absent. Polymerase chain reaction (PCR) for 56-kDa gene of Orientia tsutsugamushi using buffy coat was positive. Serum indirect immunofluorescent antibody assay was initially negative but became positive with a titer of 1:320 at follow-up, Sequencing analysis revealed the strain to be 100% identical to the TW73R strain identified in Taiwan. After the patient received doxycycline, body temperature normalized in 12 hours. Tsutsugamushi disease is one of the differential diagnoses that should be included for patients with fever who have recently returned from Southeast Asian countries. PCR for O. tsutsugamushi using patient's buffy coat was useful for early diagnosis.

SMEDDS를 이용한 난용성 약물의 용출율 향상

김계현,이윤석,배준호,지상철,박은석 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

ABSTRACT-A self-microemulsifying drug delivery system (SMEDDS) was developed to enhance the solubility and dissolution rate of poorly water soluble drug, biphenyl dimethyl dicarboxylate, DDB. The system was optimized by evaluating the solubility of DDB and the microemulsion existence range after the preparation of microemulsions with varying compositions of triacetin and surfactant-cosurfactant mixtures (Labrasol as surfactant (S) and the combination of Transcutol, Cremophor RH 40 and Plurol oleique as cosurfactant (CoS)). SMEDDS in this study markedly improved the solubility of DDB in water up to 10 mg/ml and the size of the o/w microemulsion droplets measured by dynamic light scattering showed a narrow monodisperse size distribution with an average diameter less than 50nm. The microemulsion existing range is increased proportional to the ration of S/CoS, however, it decreased remarkably as the oil content was more than 20%. In vitro dissolution study of SMEDDS showed a significantly increased dissolution rate of DDB in water (>12 fold over DDB powder), and SMEDDS also had significantly greater permeability of DDB in Caco-2 cell compared to powders.