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DNA Microarray-Based Global Transcriptional Profiling of Yersinia pestis in Multicellularity
Jingfu Qiu,Zhaobiao Guo,Haihong Liu,Dongsheng Zhou,Yanping Han,Ruifu Yang 한국미생물학회 2008 The journal of microbiology Vol.46 No.5
Yersinia pestis, the causative agent of plague, has a feature of forming multicellular aggregates at liquid-air interface around the wall of glass tube. In this study, we employed the whole-genome DNA microarray of Y. pestis to investigate the global transcriptional profile in multicellularity compared with that in its planktonic growth. A total of 177 genes were differentially expressed in Y. pestis during early stage of multicellular formation; Seventy genes of them were up-regulated while 107 down-regulated. In addition to a large number of genes encoding unknown functions, most of the induced genes encode cell envelope and transport/binding proteins. The up-regulation of amino acid biosynthesis, the differentially altered genes that are involved in virulence, and the cold shock protein genes were for the first time reported to be associated with the multicellular formation. Our results revealed the global gene expression of Y. pestis were changed in the formation of multicellularity, providing insights into the molecular mechanism of multicellular behaviour, which need investigating further.
Minimal Grasper: A Practical Robotic Grasper With Robust Performance for Pick-and-Place Tasks
JingFu Jin,Shang Li Yuen,Young Hoon Lee,ChangHyun Jun,Kim, Y. B.,Sungon Lee,Bum-Jae You,Doh, N. L. IEEE 2013 IEEE transactions on industrial electronics Vol.60 No.9
<P>In this paper, a flexible enveloping grasper is proposed for pick-and-place tasks with low manipulation and task planning complexity for practical applications. The proposed grasper has two main characteristics: self-adaptivity and flexibility. Self-adaptivity means that the proposed grasper can grip an object in a self-adaptive way such that various process complexities (e.g., sensing, force control, and sensor-motor coordination) are significantly reduced. By flexibility, we mean that, by using a flexible material, a stable grip can be implemented to cause increased friction between the grasper and the target object as a result of increased contact area. These two properties help the proposed grasper to minimize internal forces in a passive manner and to achieve successful force distribution with self-adaptivity when performing enveloping grasping. Three sets of experiments were performed with an average success rate of 93.2% in pick-and-place tasks.</P>
Wang Jingfu,Wang Jingyi,Chang Xin,Shang Jin,Wang Yuehui,Ma Qin,Shen Liangliang 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8
Streptococcus mutans is the primary causative agent of caries, which is one of the most common human diseases. Thus, rapid and early detection of cariogenic bacteria is critical for its prevention. This study investigated the combination of loop-mediated isothermal amplification (LAMP) and microfluid technology to quantitatively detect S. mutans. A low-cost, rapid microfluidic chip using LAMP technology was developed to amplify and detect bacteria at 2.2–2.2 × 106 colony-forming units (CFU)/ml and its detection limits were compared to those of standard polymerase chain reaction. A visualization system was established to quantitatively determine the experimental results, and a functional relationship between the bacterial concentration and quantitative results was established. The detection limit of S. mutans using this microfluidic chip was 2.2 CFU/ml, which was lower than that of the standard approach. After quantification, the experimental results showed a good linear relationship with the concentration of S. mutans, thereby confirming the effectiveness and accuracy of the custom-made integrated LAMP microfluidic system for the detection of S. mutans. The microfluidic system described herein may represent a promising simple detection method for the specific and rapid testing of individuals at risk of caries.
Yanan Li,Jingfu Wang,Yuting Wu,Biao Lei,Ruiping Zhi,Lili Shen 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1
A thermodynamic model of a water-lubricated single-screw air compressor was established to examine the impact of water injection parameters on performance. Heat transfer and leakage between humid air and water was considered by analyzing the impacts of rotation speed, discharge pressure, the rate of water injection, and diameter of droplets on the performance of a compressor. The discharge temperature could be reduced by increasing the rate of water injection, resulting in the compression procedure moving towards an isothermal state. The increase in the rate of water injection under rated conditions from 60 L/min to 80 L/min resulted in a reduction in the compressor discharge temperature, increased the volume efficiency, and increased adiabatic efficiency by 11.1 K, 1.5 %, and 2.8 %, respectively. Water injection atomization increased the area of transfer of heat between humid air and water and improved the performance of compressor.
Xiping Liu,Jingfu Jin,Mei Jia 한국전산응용수학회 2012 Journal of applied mathematics & informatics Vol.30 No.1
In this paper, we study a class of integral boundary value problems for fractional differential equations. By using some fixed point theorems, the results of existence of at least three positive solutions for the boundary value problems are obtained.
Camera relative pose estimation for visual servoing using quaternions
Fathian, Kaveh,Jin, Jingfu,Wee, Sung-Gil,Lee, Dong-Ha,Kim, Yoon-Gu,Gans, Nicholas R. Elsevier 2018 Robotics and autonomous systems Vol.107 No.-
<P><B>Abstract</B></P> <P>We present a novel approach to estimate the rotation and translation between two camera views from a minimum of five matched points in the images. Our approach simultaneously recovers the 3D structure of the points up to a common scale factor, and is immune to a variety of problems that plague existing methods that are based on the Euclidean homography or Essential matrix. Methods based on homography only function when feature points are coplanar in 3D space. Methods based on the Essential matrix often lose accuracy as the translation between two camera views goes to zero or when points are coplanar. By recovering the rotation and translation independently using quaternions, our algorithm eschews the shortcomings of these methods. Moreover, we do not impose any constraints on the 3D configuration of the points (such as coplanar or non-coplanar constraints). Our method is particularly well-suited for Position-Based Visual Servoing (PBVS) applications. Investigations using both simulations and experiments validate the new method. Comparisons between the proposed algorithm and the existing algorithms establish that our algorithm is robust to noise. A Matlab implementation of our algorithm is available online and free.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Quaternion formulation for the relative camera pose estimation problem. </LI> <LI> Estimating relative camera motion from captured images with high robustness to noise. </LI> <LI> Finding all solutions of a multi-variable polynomial system. </LI> <LI> Comparing state-of-the-art five point camera pose estimation algorithms. </LI> <LI> Vision based robot navigation via position based visual servoing. </LI> </UL> </P>
Liu, Xiping,Jin, Jingfu,Jia, Mei The Korean Society for Computational and Applied M 2012 Journal of applied mathematics & informatics Vol.30 No.1
In this paper, we study a class of integral boundary value problems for fractional differential equations. By using some fixed point theorems, the results of existence of at least three positive solutions for the boundary value problems are obtained.